Evaluation of the Effects of Different Basal Medium and Plant Growth Regulators on in Vitro Growth of Hazelnut

علوم باغبانی

Article 11, Volume 30, Issue 3, September 2016, Pages 417-422   

Document Type: Research Article

DOI: 10.22067/jhorts4.v30i3.37852

Authors

Parisa Daryani¹; Naser Zare^{* 1}; Esmaeil Chamani¹; Parisa Sheikhzade Mosaddegh²; Davoud Javadi Mojaddad³

¹University of Mohaghegh Ardabili

²Mohaghegh Ardabili University

³Gilan Agricultural and Natural Resources Research Center

Abstract

In this research, the effects of different basal medium and plant growth regulators on in vitro establishment and growth of hazelnut were investigated. For this, the spring apical and auxiliary buds of cv. Fertile were sterilized and cultured on NRM, MS and 1/2MS basal media containing 0.01 mg/l IBA and different levels of BAP. The results indicated that percentage of explant growth (shooting), number of leaves per explant and shoot length influenced significantly by basal media and concentration of plant growth regulator. Based on orthogonal contrasts analysis, although the highest percentage of shooting was obtained on MS medium, shoot length of explants cultured on NRM basal medium were significantly higher than those of MS and 1/2MS. The best growth response of explants in establishment stage (50% shooting, 5.33 leaves per shoot and 1.6 cm shoot length) were obtained with NRM medium supplemented with 0.01 mg/l IBA and 1 mg/l BAP. Shoots derived from establishment stage were cut to single-node explants and transferred on NRM medium supplemented with 0.05 mg/l IBA and different levels of BAP and TDZ. The highest percentage of explants growth with lowest callgenesis and explnt browning were obtained on NRM medium containing 0.05 mg/l IBA and 5 mg/l BAP.

Keywords

Apical and auxiliary buds; In vitro micropropagation; Corylus avellana; Plant tissue culture

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