Evaluation of Vermicompost and Nitrogen Biofertilizer Effects on Flowering Shoot Yield, Essential Oil and Mineral Uptake (N, P and K) in Summer Savory (Satureja hortensis L.)

بوم شناسی کشاورزی

Article 7, Volume 9, Issue 4 - Serial Number 34, December 2017, Pages 1149-1167   

Document Type: Scientific - Research

DOI: 10.22067/jag.v9i4.54711

Authors

Mohammad Reza Haj Seyed Hadi^* ; Mohammad Taghi Darzi

Department of Agronomy, Faculty of Agriculture, Roudehen Branch, Islamic Azad University, Roudehen, Iran

Abstract

Introduction
Summer Savory (Satureia hortensis L.) is the representative plants of Lamiaceae family, which has high significant nutritional and taste values. Its pharmacopoeial raw material is herb (Satureiae herba), which contains many different biologically active compounds beside essential oil and minerals like calcium, potassium, magnesium, iron, and zinc. Sustainable agricultural systems has became an important issue throughout the world. It is obvious that intensive cultivation has led to a rapid decline in organic matter and nutrient levels as well as affecting the physical soil properties . The biofertilizers practice (such as vermicompost and biological nitrogen fixing bacteria) has been recognized for a long time as an effective means of improving the structure and fertility of the soil, increasing the microbial diversity, activity and population, improving the water storage capacity of soils and crop yield.
Materials and Methods
This investigation was conducted at agricultural research fields of RAN Company at Firouzkuh, Iran in 2015. Factorial experiment based on randomized complete blocks design with two factors and three replications were chosen as an experimental design. The factors were biological nitrogen fertilizer at three levels of Control, Nitroxine and Supernitroplus, and vermicompost at four levels 0, 5, 10 and 15 ton per hectare. Measured traits consisted of fresh and dry plant weight, flowering shoot yield, essential oil content, nitrogen, phosphorus and potassium percentage in aboveground shoots. All data were subjected to statistical analysis (one-way ANOVA) using SAS software. Duncan’s multiple range test (DMRT) at 5% probability level were performed to calculate means of comparison. Data were transformed when necessary before analysis to satisfy the assumptions of normality.
Results and Discussion
Results showed that the highest fresh and dry plant weight (41.10 and 12.93g/plant, respectively) and essential oil content (2.01%) were obtained by using 10 ton per hectare of vermicompost. Applying 15 ton per hectare of vermicompost caused maximum flowering shoot yield (2237.82kg/ha), nitrogen (2.21%) and phosphorus (0.52%) content in the aboveground shoot. Mean comparison indicated that seed inoculation with Supernitroplus had significant effects on all measured traits except for nitrogen content in aboveground shoot. The maximum fresh and dry plant weight (40.21 and 14.42g/plant), flowering shoot yield (2406.21kg/ha), essential oil (2.05%), phosphorus (0.49%) and potassium (1.34%) were obtained by applying Supernitroplus. The maximum nitrogen content (2.23%) was obtained by using Nitroxine. Results of interaction effects showed that using supernitroplus and 15 ton per hectare of vermicompost caused maximum potassium content (1.50%) in Savory shoots.
Several types of studies have shown a beneficial effect on crop plants by inoculation of seeds with Azospirillum and Azotobacter strains. Inoculation of plants with Azospirillum and Azotobacter can results in significant changes in various plant growth parameters. Positive effects of inoculation have been demonstrated on including increase in root length and nutrition (NO3- , NH4+, P042- , K +, Rb+ and Fe++) uptake. Nitrogen and phosphorus are the two major plant nutrients and combined inoculation of nitrogen fixers and PSM may benefit the plants better than either group of organisms alone. Interaction studies have been done both in vitro and in vivo. Nitrogen fixers and PSM when inoculated together colonized the rhizosphere and enhanced the growth of crops by providing it with nitrogen and phosphate, respectively. Vermicompost contains most of the plant nutrients such as nitrate, phosphates, exchangeable calcium and soluble potassium, and microelements which result in improved plant growth and development and is responsible for increased qualitative and quantitative yield of many crops.
Conclusion
The results of current experiment showed that vermicompost and nitrogen fixing bacteria have stimulatory effects on the yield, essential oil and mineral elements (N, P and K) uptake by summer Savory and have thus considerable potential for providing nutritional elements in Savory production, especially for the sustainable production systems.

Keywords

Biofertilizer; Nitroxine; Summer Savory; Supernitroplus

References

Abdou, M.A.H., El Sayed, A.A., Badran, F.S., and El Deen, R.M.S. 2004. Effect of planting density and chemical and biofertilization on vegetative growth, yield and chemical composition of fennel (Foeniculum vulgare Miller): I - Effect of planting density and some chemical (Nofatrein) and biochemical (Biogen) fertilizers. Annals of Agricultural Science 42(4): 1907-1922.

Abo-Baker A.A., and Gehan, G. M. 2011. Effect of bio-and chemical fertilizers on growth, sepals yield and chemical composition of hibiscus sabdariffa at new reclaimed soil of south valley area. Asian Journal of Crop Science 3: 16-25.

Acimovic, M.G., Dolijanović, K.Z., Oljača, S.I., Kovačević, D.D., and Oljačam, M.V. 2015. Effect of organic and mineral fertilizers on essential oil content in caraway, anise and coriander fruits. Acta Scientiarum Polonorum Hortorum Cultus 14(1): 95-103.

Ahmadpoor Sefidkoohi, A., Ghajar Sepanlou, M., and Bahmanyar, M.A. 2012. The effects of long -term application of organic and inorganic fertilizer on amount of nitrogen, phosphor and potassium and growth characteristics of wheat. Journal of Sustainable Agricultural and Production Science 22(4): 71-86. (In Persian with English Summary)

Albaenell, E., Plaixats, J., and Cabrero, T. 1988. Chemical changes during vermicomposting (Eisenia foetida) of sheep manure mixed with cotton industrial wastes. Biology and Fertility of Soils 6: 266-269.

Alikhani, H.A., and Mohamadi, L. 2008. Comparison between physic-chemical characteristics of vermicompost and cold compost and their effects on growth indices in tomato. Iranian Journal of Agricultural Science 39(1): 201-207. (In Persian)

Alizadeh Sahzabi, A., Sharifi Ashorabadi, E., Shiranirad, A.H., Bigdeli, M., and Abaszadeh, B. 2007. The effects of different methods and levels of using nitrogen on some quality and quantity characteristics of Satureja hortensis L. Iranian Journal of Medicinal and Aromatic Plants 23(3): 417-431. (In Persian with English Summary)

Amirabadi, M., Seifi, M., Rejali, F., and Ardakani, M.R. 2012. Study the concentration of macro elements in corn (Zea mays L.) affected by Mycorhyza and Azotobacter chroococcum inoculation and various amounts of nitrogen. Journal of Agroeciology 4(1): 33-40. (In Persian)

Amjazi, H., and Hamidpour, M. 2012. Effects of phosphorus, vermicompost and natural zeolite on quantitative and qualitative characteristics of Zinnia elegans. Journal of Science and Technology of Greenhouse Culture 3(10): 79-86. (In Persian)

Andre, S., Neyra, M., and Duponnois, R. 2003. Arbuscular mycorrhizal sysmbiosis changes the colonization pattern of Acacia tortilis spp. Raddiana rhizosphere by two stains of rhizobia. Microbial Ecology 45: 137-144.

Ansari, S., Sarikhani, M.R., and Najafi, N. 2015. Inoculation effect of common biofertilizers on growth and uptake of some elements by bean (Phaseolus vulgaris L.) in presence of soil indigenous microflora. Journal of Sustainable Agricultural and Production Science 25(1): 85-98. (In Persian with English Summary)

Ansarifar, M., Noormohamadi, G., Haj Seyed Hadi, M.R., and Riazi, G., 2012. Effect of organic nutrients on flower yield and oil content of chamomile (Matricaria chamomilla L.). Journal of Medicinal Plants and By-products 2: 177-181.

Anwar, M., Patra, D.D., Chand, S., Alpesh, K., Naqvi, A.A., and Khanuja, S.P.S. 2005. Effect of organic manures and inorganic fertilizer on growth, herb and oil yield, nutrient accumulation, and oil quality of French basil. Communications in Soil Science and Plant Analysis 36(13-14): 1737-1746.

Arancon, N., Edwards, C.A., Bierman, P., Welch, C., and Metzger, J.D. 2004. Influences of vermicomposts on field strawberries: 1. Effects on growth and yields. Bioresource Technology 93: 145-153.

Arancon, N., Edwards, C.A., Bierman, P., Metzger, J.D., and Lucht, C. 2005. Effects of verrnicornposts produced from cattle manure, food waste and paper waste on the growth and yield of peppers in the field. Peclobiologia 49(4): 297‌-306.

Atiyeh, R.M., Arancon, N., Edwards, C.A., and Metzger, J.D. 2002. The influence of earthwormprocessed pig manure on the growth and productivity of marigolds. Bioresource Technology 81: 103-108.

Azzaz, N.N., Hassan, E., and Hamad, E.H. 2009. The chemical constituent and vegetative and yielding characteristics of fennel plants treated with organic and bio- fertilizer instead of mineral fertilizer. Australian Journal of Basic and Appllied Sciences 3(2): 579-587.

Badran, F.S., and Safwat, M.S. 2004. Response of fennel plants to organic manure and bio-fertilizers in replacement of chemical fertilization. Egyptian Journal of Agricultural Research 82(2): 247-256.

Bashan, Y., and Holguin, G. 1997. Azospirillum-plant relationships: environmental and physiological advances (1990-1996). Canadian Journal of Microbiology 43: 103-121.

Cacciari, I., Lippi, D., Pietrosanti, T., and Pietrosanti, W. 1989. Phytohormone-like substances produced by single and mixed diazotrophic cultures of Azospirillum and Arthrobacter. Plant and Soil 115: 11-153.

Candellas, L.P., Olivares, F.L., Okorokova, A.L., and Facanha, A.R. 2002. Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence, and plasma H-ATPase activity in maize roots. Plant Physiology 130: 1951-1957.

Chaoui, H.I., Zibilske, L.M., and Ohno, T. 2003. Effects of earthworm casts and compost on soil microbial activity and plant nutrient availability. Soil Biology and Biochemistry 35: 295-302.

Darzi, M.T., Ghalavand, A., Sefidkon, F., and Rejali, F. 2008. Effects of vermicompost and biophosphate fertilizer on quantity and quality of essential oil of fennel. Iranian Journal of Medicinal and Aromatic Plants 4(24): 396-413. (In Persian with English Summary)

Darzi, M.T., Ghalavand, A., and Rejali, F. 2009. The effects of biofertilizers application on N, P, K assimilation and seed yield in fennel (Foeniculum vulgare Mill.). Iranian Journal of Medicinal and Aromatic Plants 25(1): 1-19. (In Persian with English Summary)

Darzi, M.T., Haj seyed Hadi, M.R., and Rejali, F., 2012. Effects of the application or vermicomposting and nitrogen fixing bacteria on quantity and quality of the essential oil in dill. Journal of Medicinal Plants Research 6(21): 3793- 3799.

Darzi, M.T., Hadj Seyed Hadi, M.R., and Rejali, F. 2013. Effects of vermicompost and phosphatic biofertilizer application on quantity and quality of essential oil in anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants 29(3): 584-594. (In Persian with English Summary)

Dehdashtizadeh, B., Arouee, H., Azizi, M., and Davarinejad, G.H. 2010. Influence of different levels of vermicompost and phosphorus element on growing and absorbing of NPK in pepper transplant. In The 5th Congress on New Ideas in Agriculture, Khurasgan, Isfahan, Iran, 16-17 February 2010. (In Persian with English Summary)

Dzida, K., and Jarosz, Z. 2006. Influence of nitrogen-potassium fertilization on the yield and on the nutrients content in Satureja hortensis L. Acta Agrophysica 7: 879-884.

Ebadi, M.D., Rahmati, M., Azizi, M., and Hassanzadeh Khayyat, M. 2011. Effects of different drying methods (natural method, oven and microwave) on drying time, essential oil content and composition of savory (Satureja hortensis L.). Iranian Journal of Medicinal and Aromatic Plants 26(4): 477-489. (In Persian with English Summary)

Emami, A. 1996. Methods of Plant Analysis (Vol. I). Publication No. 982, Soil and Water Research Institute, Agricultural Research and Education Organization, Ministry of Agriculture, Tehran, Iran. (In Persian)

Eskandari, M., and Astaraei, A.R., 2007. Effects of various organic matters on growth characteristics and biomass production and seed yield of chickpea. Iranian Journal of Field Crops Research 5(1): 19-27. (In Persian)

Eslami Khalili, F., Pirdashti, H., Bahmanyar, M.A., and Taghavi Ghsemkheili, F. 2014. Effect of organic and chemical fertilizer on soil properties and nutrient concentration in pot marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants 30(3): 476-485. (In Persian with English Summary)

Fallahi, G., Koocheki, A.R., and Rezvani Moghaddam, P. 2009. Effects of organic fertilizers on quantitative indices, essential oil and chamazulene content in chamomile. Water, Soil and Plant in Agriculture 8: 157-168. (In Persian)

Faraji Mehmani, A., Esmaeeilpour, B., Sefidkon, F., Abaszadeh, B., Khavazi, K., and Ghanbari, A.R. 2015. Effects of biofertilizers inoculation on growth characteristics and yield of savory (Satureja hortensis L.). Journal of Agroecology 6(4): 870-879. (In Persian)

Gharib, F.A., Moussa, L.A., and Massoud, O.N. 2008. Effect of compost and bio-fertilizers on growth, yield and essential oil of Sweet Marjoram (Majorana hortensis) plant. International Journal of Agriculture and Biology 10(4): 381-387.

Ghazi Manas, M., Banj Shafiee, S., Haj Seyed Hadi, M.R., and Darzi, M.T. 2013. Effects of vermicompost and nitrogen on quantitative and qualitative yeild of chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants 29(2): 269-280. (In Persian with English Summary)

Gholami Sharafkhane, A., Jahan, M., Banayan Aval, M., Koocheki, A., and Rezvani Moghaddam, P. 2015. Effects of organic and chemical fertilizers on some agroecological characteristics, yield and essential oil of savoy (Satureja hortensis L.) in Mashad condition. Journal of Agroecology 7(2): 179-189. (In Persian)

Godara, A.S., Gupta, U.S., Lal, G., and Singh, R. 2014. Influence of organic and inorganic source of fertilizers on growth, yield and economics of coriander (Coriandrum sativum L.). International Journal of Seed Spices 4(2): 77-80.

Hadian, J., Asgari Lajayer, H., Motesharezadeh, B., and Ghorbanpour, M. 2015. Evaluation of essential oils content and yield of Satureja hortensis in response to different copper and zinc treatments. Iranian Journal of Plant Biology 7(24): 53-66. (In Persian with English Summary)

Haj Seyed Hadi, M.R., Darzi, M.T., Ghandehari, Z., and Riazi, G. 2011. Effects of vermicompost and amino acids on the flower yield and essential oil production from Matricaria chamomile L. Journal of Medicinal Plants Research 5(23): 5611-5617.

Hasanabadi, T., Ardakani, M.R., Rejali, F., and Paknejad, F. 2012. Effect of nitrogen fixation and solubilizing phosphate inoculation on yield and nitrogen uptake indices of barley (Hordeum vulgare L.) under different levels of nitrogen. Journal of Agronomy and Plant Breeding 8(3): 161-174. (In Persian)

Hasanzadeh Aval, T., Koocheki, A., Khozaei, H.R., and Nassiri Mahallati, M. 2012. Effects of density on physiological indices of summer savoy (Satureja hortensis L.) and Iranian clover in mixed cropping. Iranian Journal of Field Crop Research 10(1): 75-83. (In Persian)

Heyderianpour, M.B., Sameni, A.M., Sheikhi, J., Karimian, N., and Zarei, M. 2014. Effect of vermicompost and nitrogen on growth, concentration, and uptake of nutrients in sunflower. Journal of Water and Soil Science 18(67): 217-227. (In Persian)

Hossaini, S.M., Aghaalikhani, M., Sefidkon, F., and Ghalavand, A. 2015. Vegetative and essential oil yields of savory (Satureja sahendica Bornm.) affected by vermicompost and redroot pigweed (Amaranthus retroflexux L.) competition. Iranian Journal of Medicinal and Aromatic Plants 31(2): 342-356. (In Persian with English Summary)

Ishizuka, J., 1992. Trends in biological nitrogen fixation research and application. Plant and Soil 11:197-209.

Jahanshahi, S., Zadehbagheri, M., and Aboutalebi, A. 2012. Effect of vermicompost, azotobacter and barvar II on some quantitative and qualitative traits of coriander (Coriandrum sativum L.) medicinal plant. Journal of Crop Production Research 4(4): 391-401. (In Persian with English Summary)

Kader, M.A., Mian, M.H., and Hoque, M.S. 2002. Effects of azotobacter inoculant on the yield nitrogen uptake by wheat. Journal of Biological Science 2(4): 250-261.

Kapoor, R., Giri, B., and Mukerji, K.G. 2004. Improved growth and essential oil yield and quality in Foeniculum vulgare Mill on mycorrhizal inoculation supplemented with P-fertilizer. Bioresource Technology 93: 307-311.

Kartikeyan, B.C., Abdul Jaleel, G.M., Lakshmanan, A., and Deiveekasundaram, M. 2008. Studies on rhizosphere microbial diversity of some commercially important medicinal plants. Colloids and Surfaces B: Bionterfaces 62: 143-145.

Khalesro, S., Ghalavand, A., Sefidkon, F., and Ashgharzadeh, A. 2012. The effect of biological and organic inputs on quantity and quality of essential oil and some elements content of anise (Pimpinella anisum L.). Iranian Journal of Medicinal and Aromatic Plants 27(4): 551-560. (In Persian with English Summary)

Khan, M.S., and Zaidi, A. 2007. Synergistic effects of the inoculation with plant growth promoting rhizobacteria and an Arbuscular mycorrhizal fungus on the performance of wheat. Agricalture and Forestry 31(6): 355-362.

Khorramdel, S., Koocheki, A., Nassiri Mahallati, M., and Ghorbani, R. 2008. Application effects of biofertilizers on the growth indices of black cumin (Nigella sativa L.). Iranian Field Crop Research 6(2): 285-293. (In Persian)

Koocheki, A., Tabrizi, L., and Ghorbani, R. 2008. Evaluation of biofertilizers effects on growth characteristics, yield and qualitative characteristics of hyssop (Hyssopus officinalis L.). Iranian Journal of Field Crop Research 6: 127-137. (In Persian)

Kumari, A. 2014. Effect of vermicompost and FYM on pharmacological activities of Cannabis sativa. International Journal of Agriculture Innovations and Research 3(2): 422-428.

Leake, G., Gaspar, F., and Santos, R. 2003. The effect of water on the solubility of essential oils in dense CO2. Journal of Essential oil Research 15(3): 172-177.

Leithy, S., El-Meseiry, T.A., Abdallah, E.F. 2006. Effect of biofertilizer, cell stabilizer and irrigation regime on rosemary herbage oil yield and quality. Journal of Applied Sciences Research 2(10): 773-779.

Mafakheri, S., Omidbaigi, R., Sefidkon, F., and Rejali, F. 2012. Effect of vermicompost, biophosphate and azotobacter on quantity and quality of essential oil of Dracocephalum moldavica L. Iranian Journal of Medicinal and Aromatic Plants 27(4): 596-605. (In Persian with English Summary)

Mahfouz, S.A., and Sharaf-Eldin, M.A. 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International Agrophysics 21(4): 361-366.

Makkizadeh Tafti, M., Chaichi, M., Nasrollahzadeh, S., and Khavazi, K. 2012. Effect of different types of nitrogen fertilizers on quantitative and qualitative characteristics of Satureja hortensis L. Iranian Journal of Medicinal and Aromatic Plants 28(2): 330-341. (In Persian with English Summary)

Mohammadpour Vashvaei, M., Ghanbari, A., and Fakheri, B.A. 2015. Effect of combined feeding system on N, P and K concentration, biochemical characteristics and calyxes yield of roselle (Hibiscus sabdariffa L.). Iranian Journal of Filed Crop Science 46(3): 518-497. (In Persian)

Mohanty, S.S., Patil, C.P., Swamy, G.S.K., and Patil, P.B. 2006. Effect of vesicular-arbuscular mycorrhiza fungi and vermicompost on drought tolerance in papaya. Mycorrhiza News 16(3): 12-13.

Mohtadi, M., Mirhadi, M.J., Chraty, A., and Bahadori, M. 2015. Evaluation of the effects of bio fertilizers containing non symbiotic nitrogen fixing and phosphate solubilizing bacteria on quantitative and qualitative traits of wheat. Iranian Journal of Field Crop Research 13(4): 700-714.

Mumivand, H., Nooshkam, A., Moseni, A., and Babalar, M. 2013. Influence of nitrogen and calcium carbonate application rates on nitrate accumulation and yield of summer savory (Satureja hortensis L.). Electronic Journal of Crop Production 6(2): 109-124. (In Persian with English Summary)

Naiji, M., and Soori, M.K. 2015. Evaluation of growth and yield of savory (Satureja hortensis) under organic and biological fertilizers toward organic production. Journal of the Plant Production 38(3): 93-103. (In Persian)

Ordookhani, K., Sharafzadeh, S.H., and Zare, M. 2011. Influence of PGPR on growth, essential oil and nutrients uptake of Sweet basil. Advanced in Environmental Biology 5(4): 672–677.

Orhan, E., Esitken, A., Ercisli, S., Turan, M., and Sahin, F. 2006. Effects of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contentsin organically growing raspberry. Scientia Horticulturae 111: 38-43.

Ratti, N., Kumar, S., Verma, H.N., and Gautam, S.P. 2001. Improvement in bioavailability of tricalcium phosphate to Cymbopogon martinii var. motia by rhizobacteria, AMF and Azospirillum inoculation. Microbiological Research 156: 145-149.

Rawia, A., Eid, S., Abo-sedera, A., and Attia, M. 2006. Influence of nitrogen fixing bacteria incorporation with organic and/or inorganic nitrogen fertilizers on growth, flower yield and chemical composition of Celosia argentea. World Journal of Agricultural Sciences 2: 450-458.

Reddy, P.S., Rao, T.V.S.S., Venkataramana, P., and Suryanarayana, N. 2003. Response of mulberry varieties to vesicular arbusclar mycorrhizal and Azotobacter biofertilizers inoculation. Indian Journal of Plant Physiology 8(2): 171–174.

Rezvani Moghadam, P., Amin Ghafoori, A., Bakhshaei, S., and Jafari, L. 2013. Study the effects of biological and organic fertilizers on quantitative traits and essential oil of savoy (Satureja hortensis L.). Journal of Agroecology 5(2): 105-112. (In Persian)

Rezvani Moghadam, P., Bakhshaei, S., Amin Ghafoori, A., and Jafari, L. 2014. Study the effects of fertilizers management on production of savoy (Satureja hortensis L.) in Mashad condition. Iranian Journal of Field Crop Research 12(1): 27-33. (In Persian)

Saeid Nejad, A.H., and Rezvani Moghaddam, P. 2011. Evaluation of compost, vermicompost and cattle manure application on yield, yield components and essential oil percent in cumin (Cuminum cyminum). Journal of Horticultural Science 24(2): 142-148. (In Persian)

Sainz, M.J., Taboada-Castro, M.T., and Vilarino, A. 1998. Growth, mineral nutrition and mycorrhizal colonization of red clover and cucumber plants grown in a soil amended with composted urban wastes. Plant and Soil 205: 85-92.

Sajadi Nik, R., Yadavi, A., Balouchi, H.R., and Farajee, H. 2011. Effect of chemical (urea), organic (vermicompost) and biological (nitroxin) fertilizers on quantity and quality yield of sesame (Sesamum indicum L.). Journal of Agricultural Science and Sustainable Production 21(2): 87-101. (In Persian with English Summary)

Salehi, A., Ghalavand, A., Sefidkon, F., and Asgharzade, A. 2011. The effect of zeolite, PGPR and vermicompost application on N, P, K concentration, essential oil content and yield in organic cultivation of German chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants 27(2): 188-201. (In Persian with English Summary)

Sharma, A.K. 2002. Biofertilizers for Sustainable Agriculture. Agrobios, India, 407 p

Sheikhi, J., and Ronaghi, A. 2013. Effect of salinity and vermicompost application on nutrients concentration and yield of spinach cv. Virofly in a calcareous soil. Journal of Science and Technology of Greenhouse Culture 4(13): 81-93. (In Persian)

Shivaputra, S.S., Patil, C.P., Swarny, G.S.K., and Patil, P.B. 2004. Effect of vesicular-arbuscular mycorrhiza fungi and vermicompost on drought tolerance in papaya. Mycorrhizal News 16(3): 12-13.

Sumana, D.A., and Bagyaraj, D.J. 2002. Interaction between VAM fungus and nitrogen fixing bacteria and their influence on growth and nutrition of neem (Azadirachta indica and A. Juss). Indian Journal of Microbiology 42(4): 295–298.

Tehlan, S.K., Thakral, K.K., and Nandal, J.K. 2004. Effect of Azotobacter on plant growth and seed yield of fennel (Foeniculum vulgare Mill.). Haryana Journal of Horticultural Science 33(3/4): 287-288.

Tilak, K.V.B.R., Ranganayaki, N., Pal, K.K., Saxena, A.K., Shekhar Nautiyal, C., Mittal, S., Tripathi, A.K., and Johri, B.N. 2005. Diversity of plant growth and soil health supporting bacteria. Current Science 89: 136-150.

Vande Broek, A. 1999. Auxins upregulate expression of the indole -3- pyruvate de- carboxylase gene in Azospirillum brasilense. Journal of Bacteriology 181: 1338-1342.

Wu, S. C., Caob, Z.H., Lib, Z.G., Cheunga, K.C., and Wong, M.H. 2005. Effect of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth; a greenhouse trial. Geoderma 125: 155-166.

Zaller, J.G. 2007. Vermicompost as a substitute for peat in potting media: Effects on germination, biomass allocation, yields and fruit quality of three tomato varieties. Scientica Horticultural 112: 191-199.

Zare, S., Sirousmehr, A., Ghanbari, A., and Tabatabaei, J. 2013. Essential oil change and some quantitative characteristics of savoy (Satureja Hortensis L.) under various amounts of nitrogen and compost. Iranian Journal of Field Crop Research 11(1): 191-199. (In Persian)