Preparation and characterization of some functional properties of Aroos bean (Phaseolus Vulgaris) protein isolate

مجله پژوهشهای علوم و صنایع غذایی ایران

Article 3, Volume 14, Issue 1 - Serial Number 49, March and April 2018, Pages 39-47   

Document Type: Research Article

DOI: 10.22067/ifstrj.v1395i0.57744

Authors

Sarvenaz Osia; Zeynab Raftani Amiri^*

Department of Food Science and Technology, Sari Agricultural Sciences and Natural Resources University, Iran.

Abstract

Introduction: The Phaseolus Vulgaris on a global scale of cultivation has been categorized in the first rank and considered as the seventh staple food in the world. Proteins are usually classified into 3 groups including flours with 50 to 65 percent protein, concentrate with 65 percent protein and isolates with over 90 percent protein. Protein concentrate is produced by defatted flour through removal of soluble sugars, soluble fiber and minor compounds by alcohol, water or diluted acid with pH around 4-4.8. Protein isolate is affected by further refining processes compared to protein concentrate. In addition to the importance of the nutritional properties, crop proteins have a key role as a functional agent in the formulation of food products. Solubility, water binding capacity, oil absorption capacity, emulsifying and foaming abilities are noticed as the main functional properties. One of the specific and unique varieties of beans is Phaseolus Vulgaris var (aroos bean). The aim of this study was to determine the chemical composition of flour and bean protein isolate. In addition, some of the functional properties such as emulsification properties, foaming and stability as well as the effect of pH and ionic strength on above-mentioned characteristics were investigated.

Materials and Methods: The seed of Phaseolus Vulgaris and refined soybean oil (0ila) were purchased from Sari local supermarket. Phaseolus Vulgaris protein isolate was extracted and obtained powder was kept in impervious polyethylene plastic bags against moisture and air and placed at -18 ° C until analysis. The efficiency was calculated based on the sediment. Also, chemical composition and purity of the isolates were determined. Some functional properties, including emulsifying properties and foaming capacity at pH 4, 7, 8 and 10 were tested; and impact of the ionic strength of NaCl in concentrations of 0, 0.5 and 1 molar were examined. The results of all treatments were expressed based on the average of triplicate. Means were subjected to analysis of variances (one way-ANOVA) using (SPSS Statistics version 16) software program. Differences among the mean values of the various treatments were determined by Duncan test and the significance was defined at p < 0.05. The graphs were drawn using Excel software.

Results and Discussion: Phaseolus Vulgaris had 9% protein isolate efficiency and purity of 89.06%. The amount of protein in Phaseolus Vulgaris flour was less than the amount of protein that has been reported for other crops. While in terms of purity, protein isolate was similar to legumes in other studies. Foaming ability and emulsifying properties of Phaseolus Vulgaris protein isolate at 7 = pH were 42% and 65%, respectively. The value of this parameter using exposure to alkaline region and keeping far from the isoelectric point at pH = 10 were risen to the highest amount of 70.23% and 77.33%, respectively. During 90 minutes, stability of emulsions and foams were gradually decreased. With increasing of NaCl concentrations, foaming capacity and emulsifying properties were significantly reduced. Considering the efficient functional of Phaseolus Vulgaris protein isolate, further investigation required in order to be used in the formulation of meat products, cakes, cookies, crackers, sauces and soups.

Keywords

Aroos bean; emulsifying capacity; foaming capacity; Protein isolate

References

Adebowale, K.O. and Lawal, O.S. 2004. Comparative study of the functional properties of bambarra groundnut (Voandzeia subterranean), jack bean (Canavalia ensiformis) and mucuna bean (Mucuna pruriens) flours. Food Research International, 37(4): 355-365.

Alehosseini, A., Tavakkolipoor, H., Tolooii, A. and Keyhani, V. 2011. Evaluationof of effect of the moisture on physical properties of Aroos bean. 1th National Conference on Food Science and Technology. Azad University of Ghochan.

Alibhai, Z., Mondor, M., Moresoli, Ch., Ippersiel, D., and Lamarche, F. 2005. Production of soy protein concentrates/isolates: traditional and membrane technologies. Desalination, 191(1-3): 351-358.

Alli, I., Gibbs, B.F., Okoniewska, M.K., Konishi, Y. and Dumas, F. 1993. Identification and characterization of phaseolin polypeptides in crystalline protein isolatedfrom white kidney beans (Phaseolus vulgaris). Journal of agricultural and Food Chemistry, 41:1830–1834.

Ameri shahrabi, A., Badii, F., Ehsani, M., Maftoonazad, N. and Sarmadizadeh, D. 2011. Functional and thermal properties of chickpea and soy-protein concentrates and isolates. Iranian Journal of Nutrition Sciences and Food Technology, 6 (3): 49-58.

Aremo, M. O., Olaofe, O. and Akintayo, E.T. 2007. Functional properties of some Nigerian varieties of legume seed flours and flour concentration effect on foaming and gelation properties. Journal of Food Technology, 5(2): 109-115.

Assadpour, A., Jafari, S.M., Sadeghi mahoonak, A. and Gorbani, M. 2011. Evaluation of emulsifying and foaming capacity of the legume flours and the influence of pH and ionic strength on these properties. Iranian Food Science and Technology Research Journal, 7(1): 80-91.

Bakhshi moghadam, F., Milani, E., Mortazavi, S.A. and Meshkani, S.M. 2013. Effect of extraction methods on functional properties of chickpea protein isolated. Journal of Food Science and Technology, 10(1): 11-20.

Basiri, L. Sadeghi mahoonak, A. Alami, M. and Ghorbani, M. 2011. Effect of heat on functional properties of soy bean flour. Journal of Food Science and Technology, 9(3): 119-127.

Bond, J.S. 1989. Commercially Available Proteases, Appendix II. In: Proteolytic Enzymes, A Practical Approach. R.J. Beynon and J.S. Bond, eds., IRL Press, Oxford, U.K.

Bora, P. S. 2002. Functional properties of native and succinylated lentil (Lens culinaris) globulins. Food Chemistry, 77: 171–176.

Boye, J., Zare, F. and Pletch, A. 2010. Pulse proteins: Processing, characterization, functional properties and applications in food and feed. J. Food Research International, 43: 414–431.

Chau, C. F. and Cheung, P. 1998. Functional properties of flours prepared from three Chinese indigenous legume seeds. Food Chemistry, 61(4): 429-433.

Feizi, S. Varidi, M. Zareh, F. Varidi. M.J. 2015. Effect of pH changes on functional properties of fenugreek protein isolate. Iranian Food Science and Technology Research Journal, 11(5): 521-534.

Fenema, O.R. 1996. Food Chemistry, 3rd ed, Marcel Dekker Incorporation, USA.

Gueguen, J. 1998. Overview on functional properties of grain legume components. Grain Legumes, 20(2): 13-14.

Horwitz, W. 2002. Official Methods of Analysis,17th ed., Association of Official Analytical Chemists, Inc., Gaithersburg, USA.

Iran international standard. 1987. Method of determination for total fat content cereals and cereal products, No.2862.

Iran international standard. 2000. Cereal and cereal products- pea flour-specifications and test methods, No.6950.

Kaur, M. and Singh, N. 2005. Studies on functional, thermal and pasting properties of flours from different chickpea (Cicer arietinum L.) cultivars. Journal food chemistry, 91, 403- 411.

Kaur, M., and Singh, N. 2007. Characterization of protein isolates from different Indian chickpea (Cicer arietinum L.) cultivars. Food Chemists, 102: 366–37.

Keyhani, V. and Meshkani, S.M. 2013. Effect of physical properties of edible film that based on Beans Bride protein isolate. 2nd National Conference on Food Science and Technology of Ghochan.

Khosravi, Y. Keramat,J. Hosseini, E. Keshavarz hedayati, A. and Mahmoodi, A. 2013. Evaluation on functional properties of Iranian native variety of legumes flour. Food Technology and Nutrition, 10(3): 73-80.

Kinsella, J.E. 1981. Functional properties of soy proteins. Journal of American Oil Society Chemists, 56: 242-258.

Lawal, O. S., Adebowale, K.O., Ogunsanwo, B.M. and Bankole, S. 2005. On the functional properties of globulin and albumin protein fractions and flours of African locust bean (Parkia biglobossa). Food Chemistry, 92(4): 681-691.

Lin, M. J. Y., Humbert, E. S. and Sosulski, F. W. 1974. Certain functional properties of sunflower meal products. Journal of Food Science, 39: 368–370.

Liu, K. 2004. Soybean as functional foods and ingredients. American Oil Chemist' Society press, U.S.A.

Liu, C., Wang, H., Cui, Z., He, X., Wang, X., Zeng, X., and Ma, H. 2008. Optimization of extraction and isolation for 11S and 7S globulins of soybean seed storage protein. Food Chemistry, 102: 1310–1316.

Mwasaru, A.M., Muhammad, K., Bakar, J. and Cheman, Y.B. 1999. Effect of isolation technique and conditions on the extractability, physiochemical and functional properties of pigeon pea (Cajanus cajan) and cow pea (Vigna unguiculata) protein isolates.II. Functional properties. Food Chem, 67: 445-52.

Nasri, N. and Tinay, A.H. 2007. Functional properties of fenugreek (Trigonella foenum graecum) protein concentrate. Food Chemistry, 103, 582-589.

Ogungbenle, H. N. 2008. Effects of Salt Concentrations on the Functional Properties of Some Legume Flours. Pakistan Journal of Nutrition, 7(3): 453-458.

Papalamprou, E.M., Doxastakis, G,I. and Kiosseoglou, V. 2009 . Chickpea protein isolates obtained by wet extraction as emulsifying agent. Journal of food science and Agriculture, 90: 304–313.

Paredes-Lopez, O., Ordorica-Falomir, C., and Olivares- azquez, M. R. 1991. Chickpea protein isolates: physicochemical, functional and nutritional characterization. Journal of Food Science, 56(3): 726–729.

Ravaghi, M. Mazaheri tehrani, M. Asoodeh, A. 2012. Evaluation of changes in chemical and functional properties during production of soy protein concentrate from industrial soy flour. Iranian Food Science and Technology Research Journal, 8(1): 16-25.

Sanchez-Vioque, R., Clemente, A., Vioque, J., Bautista, J. and Millan, F. 1999. Protein isolates from chickpea: chemical composition, functional properties and protein characterization. Food Chem., 64: 237-43.

Sathe, S.K. and Salunkhe, D.K. 1981. Functional properties of great northern bean proteins: emulsion, foaming, viscosity and gelation properties. J Food Science, 46: 71-5.

Vadivel, V. and Janardhanan, K. 2001. Nutritional and anti-nutritional attributes of the under-utilized legume, Cassia floribunda Cav. Food Chemistry, 73: 209-215.

Zhang, T., Jiang, B. and Wang, Z. 2006. Gelation properties of chickpea protein isolates. Food Hydrocoll, 2: 280-86.