The Effect of Plant Density and Cultivar on Morphological Characteristics, Yield and Quality Traits of Basil (Ocimum basilicum L.) in Hydroponic System

علوم باغبانی

Article 3, Volume 32, Issue 2, September 2018, Pages 263-272   

Document Type: Research Article

DOI: 10.22067/jhorts4.v32i2.60911

Authors

Farzaneh Badakhshan^{* 1}; Farideh Sedighi Dehkordi²; Seyyed Mohammad Hassan Mortazavi²

¹Shahid Chamran university of Ahvaz

²sahid chamran university of Ahvaz

Abstract

Introduction: Basil (Ocimum basilicum L.), is considered as one of the main edible crops of Lamiaceae family. In addition of consuming as a leafy vegetable, basil is known as a medicinal plant due to its aromatic and phytochemical compositions. The common edible basil has two main varieties i.e. Green and Opal. Although the production of this plant is highly happened in open farms during hot seasons, recent changes in agricultural policies in terms of year-round production, using less water and increasing the yield and quality, have encouraged the production of basil under controlled environments such as hydroponic systems. The main criteria to proceed a successful hydroponic culture are selecting proper cultivar, planting density and nutrition management. Optimum plant density alleviates the competition between plants and as a consequence, sufficient light and nutrient can lead to higher quantity and quality. This study aimed to discover the impact of plant density on the quality and yield properties of two main varieties of basil under hydroponics system.
Material and Methods: The experiment was done at Shahid Chamran University of Ahvaz during 2015-2016 growing season. A pot experiment was conducted based of Split-plot in time design with three replications. Cultivar (Green and Opal) and planting density (150, 200 and 250 plants per m2) were considered as the main and sub plot respectively. The plants were cut twice and different morphological and biochemical properties including number of leaves, leaf area, leafy parts yield, chlorophyll, carotenoids, soluble solid content, titrable acidity, total phenolic content, antioxidant capacity and essential oil content were analyzed.
Result and Discussion:The results showed that among applied treatments, the highest number of leaves (30.61), leaf area (26140 mm2) and stem fresh weight (3.28 g) were recorded for the var. Green with 150 p/m2 planting density at second cut. In contrast, this treatment had the lowest number of leaves (12.33) and leaf area (10810 mm2) at first cut. While maximum leaf fresh weight (5.84 g) was obtained for second cut of 150 p/m2 planting density, minimum leaf and stem fresh weight (3.09 and 2.01 g respectively) were found for second cut of 200 p/m2 planting density. Total fresh yield as an important criteria for a leafy vegetable was affected by both variety and planting density factors. The highest amount of yield (1427 g/m2) was recorded at second cut when plants were at 250 p/m2. In contrast, the plants of 150 p/m2 had the minimum yield at first cut (1020 g/m2). The chlorophyll content was higher in Opal variety surprisingly and the plants of 250 p/m2 and 150 p/m2 had around 2.09 mg/g chlorophyll at second cut. Similar findings were obtained for total phenolic content of leaves that was around 104.3 mg/Kg fresh weight for these treatments. Lowest levels of chlorophyll (1.29 mg/g FW) and total phenolic content (6.158 mg/kg) were seen in the leaves of var. Green when they were planted at 150 plant/m2 density. The data for total carotenoids content showed that the level of this pigments were affected by both parameters of variety and planting density. The leaves of var. Opal of 250 p/m2 density had the highest (6.252 mg/g fresh weight) carotenoids content. The highest (2.021 mmol Fe II/g FW) and lowest (0.69 mmol Fe II/g) amount of antioxidant capacity was recorded in Opal and Green varieties respectively, when they were at 150 plant/m2 density. The taste related parameters including total soluble solids, titrable acidity and essential oil content were not affected by planting density and variety. However, the level of acidity was increased by increasing plant density. On the other hand, a significant difference was seen in terms of TSS, acidity and essential oil between first and second cuts.
Conclusion: Overall and based on obtained data, it can be said that the var. Opal at 150 and 250 plants/m2 density showed the best results in terms of evaluated morphological and qualitative traits and can be recommended for hydroponics basil cultivation. Basil is harvest in 2-3 cuts and this experiment showed that for almost all vegetative parameters, the second cut had better results. The improved yield and quality at second cut could be attributed to the better establishment of roots and providing higher levels of nutrients.

Keywords

antioxidant capacity; Essential oil; Leaf area

References

1- Arnon A.N.1967.Method of extraction of chlorophyll in the plants, Agron Journal, 23:112-121.

2- Bekhradi F., Delshad M., Kashi A., Babalar M., and Ilkhani S. 2014. Effect of plant density in some basil cultivars on yield and radiation use efficiency, Journal of Biodiversity and Environmental Sciences (JBES), 5(1): 91-96.

3- Benzie I.F., and Strain J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay, Analytical biochemistry, 239(1): 70-76.

4- Biglari F., AlKarkhi A.F., and Easa A.M. 2008. Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran, Food chemistry, 107(4): 1636-1641.

5- Cao G,and Prior R.L. 1998. Comparison of different analytical methods for assessing total antioxidant capacity of human serum, Clinical Chemistry, 44:1309–1315.

6- Darzi M.T., khoda bandeh N., Haj syed hadi M. and Yasa N. 2001. The effects of planting date and plant density on seed yield and quality and quantity of active ingredient Fennel Medicinal Herb,National Conference of Iranian Medicinal Plants / Forests and Rangelands Research Institute, 151-152. (in Persian)

7- El-Gendy S.A., Hosni A.M., Ahmed S.S., Ömer E.A. and Reham M.S. 2001. Variation in herbage yield and oil composition of sweet Basil (Ocimum basilicum L.) var. ‘Grande Verde’ grown organically in a newly reclaimed land in Egypt, Journal of Agricultural Science, 9: 915 - 33.

8- Fernandes P.C., Facanali R., Teixeira J.P.F., Furlani P.R. and Marques M.O.M. 2004. Culture of basil substrata and hydroponic system under protected environmental, Horticultral Brasilaria, 22 .260-264.

9- Ganjali H.R., Ayeneh Band A., Heidari Sharif Abad H. and MoussaviNik M. 2010. Effect of sowing date, plant density and nitrogen fertilizer on yield, yield component and various traits of Calendula officinalis, American-Eurasian Journal of Agricultural and Environmental Sciences, 9(2): 149-155.

10- Ghanbari A.A., and Taheri Mazandarani M. 2003. Effects of sowing date and plant density on yield of pinto bean, Seed and Plant Journal, 19(4): 483-496.

11- Guo C., Yang J., Wei J., Li Y., Xu J. and Jiang Y. 2003. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay, Nutrition Research, 23(12): 1719-1726.

12- Haj syed hadi M., khoda bandeh N., Darzi M.T. and Yasa N. 2001. The effects of planting date and plant density on the amount of oil and Chamazulene in Chamomile Medicinal Herbs, National Conference of Iranian Medicinal Plants / Forests and Rangelands Research Institute. 119. (in Persian)

13- Javanmardi J., Stushnoff C., Locke E. and Vivanco J.M. 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions, Food Chemistry, 83(4): 547-550.

14- Kiferle C., Lucchesini M., Mensuali-Sodi A., Maggini R., Raffaelli A. and Pardossi A. 2011. Rosmarinic acid content in basil plants grown in vitro and in hydroponics, Central European Journal of Biology, 6: 946-957.

15- Letchamo W., Xu H.L. and Gosselin A. 1995. Photosynthetic potential of Thymus vulgaris selections under two light regimes and three soil water levels, Scientia Horticulture, 62: 89-101.

16- Liu Y., Perera C.O. and Suresh V. 2007. Comparison of three chosen vegetables with others from South East Asia for their lutein and zeaxanthin content, Food chemistry, 101(4): 1533-1539.

17- Maboko M.M. and Du Plooy Ch. 2013. High plant density planting of basil (Ocimum basilicum) during Summer/Fall growth season improves yield in hydroponic system, Acta Agriculture Scandinavica, Section B- Soil and Plant Science, 63(8): 748-752.

18- Majnoon hosseini N., Mohammadi H., Poustini K. and Zeinaly khanghah H. 2003. Effect of Plant Density on Agronomic Characteristics, Chlorophyll Content and Stem Remobilization Percentage in Chickpea Cultivars (Cicer arietinum L.). Iranian, Journal of Agricultural Science, 34(4): 1011-1019. (in Persian with English abstract)

19- Malakouti M.J. and Tehrani M.M. 2001. Effects of Micronutrients on Yield and Quality of Agricultural Products 'Micro Nutrients with Macro Effects'. Second edn. Tarbiat Modares University, Press, 299 p.

20- Moosavi S.G.R. 2012. Effect of sowing date and plant density on yield and yield components of roselle, Journal of Medicinal Plants Research, 6(9): 1627-1632.

21- Morteza E., Akbari G. A., Sanavi S. M. M. and Farahani H. A. 2009. Effects of sowing date and planting density on quantity and quality features in valerian (Valeriana officinalis L.), Journal of Ecology and the Natural Environment, 1(9): 201-205.

22- Naghdi badi H.A., Yazdani D., Nazari F. and Sajaid M.A. 2002. Seasonal changes, performance and composition of essential oil of thyme (Thymus vulgaris L.) The planting densities, Journal of Medicinal Plants, 5:51-56. (in Persian)

23- Omidbaigi R. 2000. Processing of medicinal plants. Mashhad. Astan Quds Razavi Publishers, Published company, (3): 99-106.

24- Pearson L. and Drost D. 2006. Basil in the Garden. Extention Utah State University, HG/Garden/ 200-601.

25- Raimondi G., Orsini F., Maggio A., De Pascale S., and Barbieri G. 2006. Yield and Quality of Hydroponically Grown Sweet Basil Cultivars, Acta Horticulturae, 723, 357-360.

26- Ramezanian A., Tavallali V. and SadeghiGhotbabadi F. 2001. Greenhouse -Scientific and practical methods of greenhouse construction and plant care, Takhtejamshid publishing, 120. (in Persian)

27- Sadeghi S., Rahnavard A., and Ashrafi, Z. 2009. The effect of plant density and sowing data on yield of basil (Ocimum basilicum L.) in Iran, Journal Science Techno Agriculture Natural Research, 5: 413–422.

28- Saglam C., Atakisi I., Turhan H., Kaba S., Arslanoglu F. and Onemli F. 2004. Effect of propagation method, plant density, and age on lemon balm (Melissa officinalis) herb and oil yield, New Zealand Journal of Crop and Horticultural Science, 32(4): 419-423.

29- Sahlin E., Savage G.P., and Lister C. E. 2004. Investigation of the antioxidant properties of tomatoes after processing, Journal of Food Composition and Analysis, 17(5): 635-647.

30- Saifi S., Neamati H., shour M. and Abedi B. 2012. The effect of plant density and pruning on yield and plant growth are two varieties of sweet pepper greenhouse, Science and Technology Journal of Greenhouse Cultures, 3(11):77-82. (in Persian)

31- Schoenstein G. 1996. Hydro-organics: growing basil during the off-season. Small Farm Today, I3(1): 9-42.

32- Seyedi A., Ebadi A., Babalar M. and Saeedi B. 2010. The effect of plant density on yield and fruit quality of strawberries Selva in soilless culture vertical, Journal of Horticultural Science, 24(1):1-6 .(in Persian)

33- Sgherri C. Cecconami S., Pinzino C., Navari-Izzo F. and Izzo R. 2010. Levels of antioxidants and nutraceuticals in basil grown in hydroponics and soil, Food Chemistry, 123, 416–422.

34- Shapiro C. A., Schepers J. S., Francis D. D. and Shanahan J. F. 2005. Using a chlorophyll meter to improve N management, Lincoln, NE: University of Nebraska-Lincoln Extension Bulletin G, 1632.

35- Smith C.A., Svoboda K.P. and Noon M.M. 1997. Controlling the Growth and Quality of Hydroponically-Grown Basil (Ocimum Basilicum L.) (Refereed), Acta Horticulturea, International symposium, Growing media and plant nutrition in horticulture, 450:479-486.

36- Srmadnya qh., and kochaki A. 1989. Crop Physiology (Translation), Mashhad Publishers Jihad Collegiate. 468. (in Persian)

37- Toor RK. and Savage GP. 2005. Antioxidant activities in different fractions of tomato, Food Research International, 38: 487-494.

38- Tüzel I.H., Tüzel Y., Gül A., Meriç M.K., Yavuz O. and Eltez R.Z. 2001. Comparison of open and closed systems on yield, water and nutrient consumption and their environmental impact, Acta Horticulturae, 554:221-228.

39- Zehtab-Salmasi S., Heidari F. and Alyari H. 2008. Effects of microelements and plant density on biomass and essential oil production of peppermint (Mentha piperita L.), Plant Sciences Research, 1(1): 24-26.

40- Žnidarčič D., Ban D. and Šircelj H. 2011. Carotenoid and chlorophyll composition of commonly consumed leafy vegetables in Mediterranean countries, Food Chemistry, 129(3): 1164-1168.