Some Plant Parasitic Nematodes of Fruit Trees in Northern Khorasan Province, Iran

پژوهش های حفاظت گیاهان ایران

Article 7, Volume 31, Issue 1 - Serial Number 35, March 2017, Pages 124-130   

Document Type: Research Article

DOI: 10.22067/jpp.v31i1.54324

Authors

N. Heidarzadeh^{* 1}; E. Mahdikhani Moghadam²; M. Pachenary²

¹Islamic azad university branch of Bojnourd

²Ferdowsi University of Mashhad

Abstract

Introduction: Nematodes (Phylum Nematoda) are considered as one of the most abundant and diverse animals on earth. They are found in terrestrial, freshwater, brackish, and marine environments and play important ecological roles in soil ecosystems. The order Tylenchida includes the largest and economically most important group of plant-parasitic nematodes so they have always received ample taxonomic attention. Many plant parasitic nematode species are important pests of fruit trees. They damage the plant by directly attacking roots and subsequently predisposing them to secondary infections by bacteria, fungi by causing replant and pre-plant problems of orchards and also by transmission of viruses. Plant parasitic nematodes feed on a plant root system, ability to take up water and minerals and to transport nutrients to the shoot. This restricts root growth reduce plant vitality and inhibits shoot growth, the combination of which results in decreased in quality and yield. The economically most important species belong to the genera Meloidogyne, Pratylenchus, criconemella, Logidorus, Xiphinema, Trichodorus and Paratrichodorus and are widely distributed in fruit orchards throughout the world. Nematode species are classically defined on the basis of these qualitative and quantitative characters. Although morphological information might help species diagnostics, these characters are homoplasious features in many cases and do not adequately consider the possibility of convergent evolution. As a result, new species descriptions are increasingly supported by molecular evidence. However, the study of morphology remains a critical necessity as morphology is the primary interface of an organism with its environment with key implications for development and ecology. Therefore, a more robust phylogeny based on a combination of morphological and molecular approaches is needed to clarify important relationships within Tylenchomorpha. The purpose of the present investigation was the identification of plant-parasitic nematodes of fruit trees based on morphological and morphometrical characters in Northern Khorasan province.
Materials and Methods: In order to investigate the biodiversity of plant parasitic nematodes of fruit cultivation in Northern Khorasan Province, 70 soil samples were collected during 2011-2012. Nematodes were extracted by centrifugal flotation technique and transferred to glycerin according to the modified De Grisse method (1969). The permanent slides were prepared from the extracted nematodes. The nematodes were identified by light microscopy, based on morphological and morphometrical characters. Measurements and drawings were performed using a drawing tube attached to an Olympus BH2 light microscope. The ratios and the morphometric symbols used in morphometric tables of each specimen. Nematodes were identified based on morphological and morphometrical characters using identification keys.
Results and Discussion: In this study, 17 species from 13 genera belonged to sub order Tylenchina were identified as follows: Aphelenchoides richardsoni, Aphelenchus avenae, Basiria graminophila, Boleodorus thylactus, Ditylenchus filimus, D. medicaginis, Filenchus cylindricaudatus, F. thornei, Geocenamus tenuidens, Helicotylenchus digonicus, H. pseudorobustus, Heterodera schachtii, Merlinius brevidens, Pratylenchus neglectus, P. thornei, Tylenchorhynchus latus, Zygotylenchus guevarai.
Among these species, Pratylenchus neglectus, Merlinius brevidens and Boleodorus thylactus were more frequent, respectively. Ditylenchus filimus is reported for the first time from Iran. D. filimus is characterized by low and striated head, stylet 7-9 µm, median bulb muscular, glandular bulb offset, posterior vulva( V=81-85), PUS=0.5-1.1, tail conical with very sharp and pointed terminus.
Conclusion: In this study, 17 species from 13 genera belong to suborder Tylenchina were identified. Among these species, Pratylenchus neglectus, Merlinius brevidens and Boleodorus thylactus had the most frequency respectively. Ditylenchus filimus is reported for the first time from Iran.

Keywords

Fruit trees; Northern Khorasan Province; Plant parasitic nematode

References

1- Agriculture Organization of North Khorasan. 2014. Statistics Plant Production: Statistics garden products: Statistics acreage production and performance of the horticultural. Available at http://boe.nkj.ir/?partid=8115&item= (visited 11 June 2015).

2- Allen M. W. 1955. A review of the nematode genus Tylenchorhynchus. University of California Publications Zoology ,61: 129-165.

3- Anderson R. V. 1983. An emended description of Ditylenchus valveus Thorne & Malek, 1968 and description of D. filimus n. sp. (Nematoda: Tylenchidae) from mushroom compost in Canada. Canadian Journal of Zoology, 61: 2319-2323.

4- Anderson R. V., and Hooper D. J. 1980. Diagnostic value of vagina structure in the taxonomy of Aphelenchus Bastian, 1865 (Nematoda: Aphelenchidae) with description of A.(Anaphelenchus) isomerus n.subgen.n.sp. Canadian Journal of Zoology, 58: 924-928.

5- Atighi M. R., Pourjam E., and Okhovat M. 2011. The Genus Filenchus in Northern Khorasan Province, Iran. Iranian Journal of Plant Protection Science, 42(2): 261-266.

6- Bahmani J., Khozeini F., Barooti S., Rezaee S., and Ghaderi R. 2013. Plant Parasitic Nematodes Associated with Walnut in The Sanandej Region of West Iran. Journal of Plant Protection Research, 53(4): 404-408.

7- Bello A., and Geraert E. 1972. Redescription of eight species belonging to the super family Tylenchoidea (Nematoda: Tylenchida). Nematologica, 18: 190-200.

8- Brzeski M. W. 1983. Three new species of Ditylenchus Filipjev, 1936 and comments on Basiroides longimatricalis Kazachenko, 1975 (Nematoda :Anguinidae). Nematologica, 29: 380-389.

9- Brzeski M. W. 1991. Review of the genus Ditylenchus Filipjev, 1936 (Nematoda: Anguinidae). Revue de Nematologie, 14(1): 9-59.

10- De Grisse A. T. 1969. Redescription ou modification de quelqutes techniques utilissisedans L. etude des Nematode phytoparasutaires. Meded Rijksfaculteit der Landbouwetenschappen Gent, 351-369.

11- Filipjev I. N., and Schuurmans Stekhoven J. H. 1941. A manual of agricultural helmintology. Brill, Leiden, 878 p.

12- Ganjipour M. 2012. Identification of plant parasitic nematodes from Rhizospher of Walnut Trees in North Khorasan Province, Iran. MSc Thesis. Islamic Azad University, Science and Research Branch, Tehran.

13- Golden A. M. 1956. Taxonomy of the spiral nematodes (Rotylenchus and Helicotylenchus) and the developmental stages and host-parasite relationships of R. buxophilus n. sp. attacking boxwood. Bulletin of the Maryland Agricultural Experiment Station, A. 85: 28 p.

14- Jahanshahi-Afshar F., Pourjam E., and Kheiri A. 2005. Tylenchids associated with Jiroft orchards and description of four species new for nematode fauna of Iran. Iranian Journal of Agricultural Science, 37(3): 529-542.

15- Jenkins W. R. 1964. A rapid centrifugal flotation technique for separating nematodes from soil. Plant Disease Reporter, 48 : 692 p.

16- Mokbel A. A., Ibrahim I. K. A., and EL-Saedy M. A. M. 2006. Plant parasitic nematodes associated with some fruit trees and vegetable crops in Northern Egypt. Journal of Phytopathology, 34(2): 43-51.

17- Perry V. G., Darling M. H., and Thorne G. 1959. Anatomy, taxonomy and control of certain spiral nematodes attacking blue grass in wisconsin. Bulletin of university of wisconsin research, 207: 1-24.

18- Rafiei S., Pourjam E., and Niknam Gh. 2010. Identification of Some Nematodes Associated with Fruit Trees in Moghan Region, Iran. Journal of Plant Protection, 24(1): 69-79.

19- Raski D. J., and Geraert E. 1986. Review of the genus Filenchus Andrassy, 1954 and description of six new species (Nemata: Tylenchidae). Journal of Nematologica, 32: 265-311.

20- Shahina F. 1996. A diagnostic compendium of the genus Aphelenchoides Fischer , 1894 (Nematoda: Aphelenchida) with some new record of the group from Pakistan. Pakistan Journal of Nematology, 14(1): 1-32.

21- Sher S. A., and Allen M. W. 1953. Revision of the genus Pratylenchus (Nematoda: Tylenchidae). University of California Publications Zoology, 57: 441- 470.

22- Siddiqi M. R. 1959. Basiria graminophila, n. g. n. sp. (Nematoda: Tylenchinae) found associated with grass roots in Aligarn, India. Nematologica, 4: 217-222.

23- Siddiqi M. R. 1970. On the plant-parasitic nematode genera Merlinius gen. n. and Tylenchorhynchus Cobb and the classification of the families Dolichodoridae n. rank. Proceedings of the Helminthological Society of Washington ,37: 68-77.

24- Siddiqi M. R. 1975. Zygotylenchus guevarai. C.I.H. Description of Plant Parasitic Nematodes, Set. 5, No. 65. Farnham Royal, UK: Common Wealth Agricultural Bureaux.

25- Steel A. E. 1986. Nematode parasites of sugar beet compendium of beet diseases and insects. APS press, American Phytopathological Society St. Paul. Minnesota: 33-35.

26- Thorne G. 1941. Some nematodes of the family Tylenchidae which do not possess a valvular median esophageal bulb. The Great Basin Naturalist, Brigham Young university, Provo, Utah. 2:37-85.

27- Thorne G., and Malek R. B. 1968. Nematodes of the Northern Great Plains, Part1. Tylenchida Nemata: Secernentea. Brookins, S. Dakota, Agric. Exp. State, Bull, 31:111 p.