Investigation Effect of Drought Stress Ievel of PEG 6000 on Seed Germination Principle and its Relation With Drought Tolerance Index in Promising Lines and Cultivares of Bread Wheat (Triticum. aestivum L.)

پژوهشهای زراعی ایران

Article 4, Volume 12, Issue 4 - Serial Number 36, January 2015, Pages 582-596   

Document Type: Research Article

DOI: 10.22067/gsc.v12i4.45141

Authors

H Abdi^{* 1}; M. R Bihamta²; E Aziz Ov³; R Choukan⁴

¹Genetics Sciences Academy of Azerbaijan Republic

²Tehran University

³University of Bako

⁴Seed and Plant Improvement Research Institute, Karaj

Abstract

In order to investigate the effects of drought stress levels on seed germination and its related traits with drought stress indices, an experiment was conducted in two sparate phases with 40 wheat genotypes under field and laboratory condition for germination stages with 4 level of the polyethylene glycol (PEG) concentrations. The experimental design was factorial based on a randomized complete block design with three replications. The measured traits were radicle length, stem length, radicle length to shoot length ratio, germination rate and germination percentage. The experiment in the filed stage was conducted with 3 replications under both normal and drought stress condition. Result of the laboratory experiment indicated that effects of stress on traits were significant. Also different between genotypes were significant. The highest germination rate and percentage was measured under normal condition with 4.22 days and 79.9% respectively. Moderate level of stress (-3 bar) of drought stress had no significant effect on radical, coleoptile, stem length and dry weight, but reduced the germination rate significantly. In high level of drought stress (-6 bars) all traits were decreased significantly expect radicle length and radicle length to shoot length ratio. In control condition, positive significant correlation was observed between all traits measured in (-6 bars) under laboratory condition, with grain yield and drought tolerance indices. But in slight (-3 bars) and severe stress dry weight and radicle length had positive significant correlation with grain yield. Grain yield of genotypes showed significant differences under field study in both normal and stress condition. Results of correlation between grain yield and drought stress indices showed that stress tolerance index (STI), mean productivity (MP) and geometric mean productivity (GMP) were suitable for the genotypes identification with highest seed yield in both normal and stress conditions according to correlation coefficients between seed and its related traits and grain yield such as correlation coefficients between drought stress indices and grain yield in both normal and stress conditions, revealed that the genotypes number 39 (WS-82-9), 38 (DN-11), 22 (mbul/Alamo/M-73-18) and 25 (Alvd/Aldan/las/3--) were determined as the most tolerant and number 5 (Gasspard) and 40 (Mahdavi) were detected as the most susceptible cultivars for drought stress.

Keywords

PEG 6000; Drought Tolerance Index; Stress; wheat; Seed Germination Principle

References

-اسکوئی، ب. 1389. اثر تنش خشکی بر بر‌خی از لاین‌های گندم در مرحله رشد رویشی، یازدهمین کنگره علوم زراعت و اصلاح نباتات ایران. دانشگاه شهید بهشتی ص123.

2- اصغری، ع. 1377. طبقه بندی ارقام گندم دیم از نظر مقاومت به خشکی. چکیده مقالات پنجمین کنگره زراعت و اصلاح نباتات ایران. مؤسسه تحقیقات اصلاح و تهیه نهال و بذر کرج. ص 254-253.

3- روستائی، م. صادق زاده اهری،د و ب. ارشدی. 1382. بررسی ارتباط صفات مؤثر بر عملکرد دانه گندم با استفاده از تجزیه به عامل‌ها در شرایط دیم. مجله دانش کشاورزی جلد یک صفحه 13.

4- کوچکی ع. راشد محصل م. و صدرآبادی، م. 1367. مبانی فیزیولوژی رشد و نمو گیاهان زراعی انتشارات آستان رضوی. 404 صفحه.

5- گراوندی، م.، فرشادفر، ع.، وکهریزی، د. 1389. ارزیابی تحمل به خشکی در ژنوتیپ‌های پیشرفته گندم در شرایط آزمایشگاه و مزرعه. فصلنامه به نژادی نهال و بذر.

6- ملکوتی، م.، ج و نبی غیبی م. 1379. تعین حد بحرانی عناصر غذایی موثر در خاک، گیاه و میوه در راستای افزایش عملکرد کمی وکیفی محصولات استراتژیک کشور. نشر آموزش کشاورزی چاپ 2. ص 34.

7- Ashraf, M., and P. J. C. Harris. 2005. Abiotic stresses: Plant resistance through breeding and molecular approaches. The Haworth Press, New York, pp, 725.

8- Azizinia., S. M. R. Ghannadha, A. A. Zali, B. Yazdi-Samadi, and A. Ahmadi. 2005. An evaluation of quantitative traits related to drought resistance in synthetic wheat genotypes in stress and non-stress conditions. Iranian Journal of Agricultural Sciences, 36: 281-293 .

9- Baalbaki, R. Z, Zuray k, R. A. Bleik, M. M, and Talhouk, S. N. 1999. Germination and seedling development of drought tolerant and susceptible wheat moisture stress. Seed Science and Technology. 27: 291-302.

10- Balouchi, H. R. 2010. Screening wheat parents of mapping population for heat and drought tolerance, Selection of wheat genetic variation. International Journal of Biological and Life Sciences. 6: 56-66.

11- Bayoumi, T. Y. H., Eid, M. H. and Metwali, E. M. 2008. Application of physiological and biochemical indices as a screening technique for drought to landrace in wheat genotypes. African journal of Biotechnol. 7(14), 2341-2352.

12- Belcher, E. W. and L. Miller. 1974. Influence of substrate moisture level on the germination of sweet gum and pin seed. Proceeding of the Association of official Seed Analysis, 65: 88-89.

13- Cattivelli, L., F. Rizza, F. W. Badeck, E, Mazzuotelli, A. Tondelli and C. Mare. 2008. Drought tolerance improvement in crop plants: An integrated view from breeding to gemomics. Fiehd Crop Reserch. 105: 1-14.

14- Dhanda, S. S., Sethi, G. S., and Behl, R. K. 2004. Indices of drought tolerance in wheat genotypes at early stages of plant growth. Journal of Agronomy and Crop Science volume 19, Issue 1. Pages 6-12.

15- Fernandez, G. C. J. 1992. Effective selection criteria for assessing plant stress tolerance. In: Proceeding of the Sympozium. Taiwan, 13-16 Aug. 1992. By C. G. Kno. AVRDC.

16- Fischer, R. A., and Maurer, R. 1978. "Drought resistance in spring wheat cultivars, I. Grain yield responses Tour. Agricience. Reserch: 29: 897-912.

17- Golabadi, M., Arzani, A., and Mirmohamadi maibody, S. A. M. 2006. Assessment of drought tolerance in segregation population in durum wheat. African Journal of Agricultural Research 1: 162-17.

18- Hussain, S. S. 2006. Molecular breedhng for abiotic steress tolerance: droght perspective. Procpakistan Academic Science, 43(3),189-210.

19- Metwali, M. R. Ehab; Manal, H. Eid. and Tarek,Y. Bayoumi. 2011. Agronomical traits and biochemical genetics markers associated with salt tolerance in wheat cultivars (Triticum aestivumL.). Australian journal of Basic and Applied Sciences, Volume 5 lssue5, pages 174.

20- Michel, E. B, and Kaufman. M. K.1974. The osmotic potential of polyethylen glycol 6000. Plant. Physiology. 51: 914-916.

21- Nourmand. F., Rostami. M. A., and Ghannadha, M. R. 2001. Evaluation of drought resistance indices in bread wheat (triticum aestivum L). Iranian Journal Agricalture Science. 32: 4.7, 5-805.

22- Ouk, M., Basnayake, J., Tsubo, M., Fukai, S., Fischer, K. S., Cooper. M., and Nesbitt, H. 2006. Use of drought response index for identification of drought tolerant genotypes in rainfed lowland rice. Field Crop Research 99: 48-58.

23- Pan, X. Y., Y. F. Wang, G. X. Wang, Q. D. Cao and J. Wang. 2002. Relationship between growth redundancy and size inequality in spring wheat populations mulched with clear plastic film. Acta Phytoecol. Sinica. 26: 177-184.

24- Rauf, M., Munir, M. U-Hassan, M. Ahmed, and Afzai, M. 2007. Performance of wheat genotypes under osmotic stress at germination and early seedlig growth stage. African journal of Biotechology. 8, 971- 975.

25- Saeidi, M., Ahmadi, A., Postini, K., and Jahansooz, M. R. 2007. Evaluation of germination traits of different genotypes of wheat in osmotic stress situation and their correlations with speed of emergence and drought tolerance in Farm situation. Journal of Science and Technology of Agriculture and Natural Resources 11: 281-293 (in Farsi).

26- Seefeldet, S. S., Kidwell, K. K. and Waller, J. E. 2002. Base growth temperature, germination rates and growth response of contemporary spring wheat (Triticum aestivum L.) cultivars from the USA Pacific North West. Field Crops Reserch. 75: 45-52.

27- Sio-Se Mardeh, A., Ahmadi, A., Poustini, K. and Mohammadi, V. 2006. Evaluation of drought resistance indices under various environmental conditions. Field Crops research, 98: 222-229.

28- Soltani, A., Zeinali, E., Galeshi, S., and Latifi, N. 2001. Genetic variance for and interrelationship among seed vigor traits in wheat from the Caspian Sea Coast of Iran Seed Science andTechnology. 29: 653-662.

29- Zarei, L., Farshadfar, E., Haghparast, R., Rajabi, R., and Mohammadi SarabBadieh, M. 2007. Evaluation of some indirect traits and indexes to identify drought tolerance in bread wheat (Triticum aestivum L.). Asian Journal of Plant Science 6: 1204-1210.