Effect of Melilotus officinalis, Oliveria decumbens Vent and Aloe vera L on production performance, biochemistry characteristics and meat microbial count in Japanese quail

پژوهشهای علوم دامی ایران

Article 6, Volume 12, Issue 2 - Serial Number 42, June 2020, Pages 211-222   

Document Type: Poultry Nutrition

DOI: 10.22067/ijasr.v12i2.78238

Authors

Hassan Habibi^{* 1}; Najme Ghahtan²

¹Department of Animal Sciences, Faculty of Agricultural and Natural Resources, Persian Gulf University, Bushehr, Iran.

²Department of Horticultural Sciences , Faculty of Agriculture and Natural Resources, Persian Gulf University Bushehr, Iran

Abstract

Introduction[1] Development of poultry production causes increasing demand for feed ingredients and also ways of manipulating poultry diets in order to increase feed conversion ratios for maximum production. In this regard, use of feed additives and growth promoters has been increased. Antibiotic growth promoters have been successfully used at sub therapeutic doses in poultry production to promote growth and protect health of the birds. The use of antibiotics as growth promoters has been prohibited by many countries as they have different side effects on both bird and human health. There has been an increasing trend towards using natural feed additives to improve the performance, increase the dietary protein, energy utilization and to maintain health of birds. Herbs, botanicals, essential oils, oleoresins and algae are alternatives for antibiotics and which have not been found to causes antibiotic-like side effects on consumers. The beneficial effects of herbal plants additives in poultry (broilers, layers, broiler breeders, layer breeders and quail) may arise from their having positive effect on feed intake, digestive secretions, immune stimulation, antibacterial, antibacterial, coccidiostatic, antiviral or anti-inflammatory activity. In plant tissues, pH values are dependent on the presence of poly-carboxylic acids, phosphate salts, fiber and proteins. In this study, we partly replaced quail meal with very low amounts of Melilotus officinalis, Oliveria decumbens vent and Aloe Vera L meal to evaluate the medicinal plants as a Quail meal supplement and to assess the effects of this dietary change on growth performance, Biochemical factors, Qualitative agent and microbial load in Japanese quail meat.
Materials and methods A total of 280 Japanese Quail hatchlings (one-day-old at test inception) were used in the study. Treatment protocol used four replicates each of eight different tests either of two levels (0.5 or 2% of total food mass) of tested medicinal plant material and one control group that received a diet free of medical plant supplements. Each treatment had four replicates, thus there was a total of 28 groups of 10 birds. The hatchling birds were maintained and adapted for 15 days under lab conditions: 16-h lighting, 8 h darkness, at temperature of 25 °C ± 2. Feed (mash form) and water were provided ad libitum throughout the whole trial. Body weight gain per hen were evaluated at 27 and 42 d. Feed intake and feed conversion ratio were determined and calculated during each phase of whole period. At the end of the experimental period, 4 Quail from each replicate of different groups were randomly selected and sacrificed to calculate the carcass. Serum triglycerides (TG), cholesterol (Cho), High Density Lipoprotein (HDL) and Low Density Lipoprotein (LDL) cholesterol levels were determined. The meat samples were dried for 24h in an oven at 70 ˚C and the meat moisture was calculated. The meat samples were placed after centrifugation for 24h in an oven at 70 ˚C and the meat water holding capacity was calculated. The ash content was determined by charring followed by ashing the samples at 720°C to a white ash. The pH of the meat samples was determined by homogenizing 10 g of meat with 50 mL of distilled water. The homogenates were filtered, and the pH of each sample was measured with a pH meter at room temperature. The samples were analyzed for the total bacterial and coliforms load using plate count agar by PCA and Mac Conkey technique. The subjective evaluations of product quality were carried out by an experienced panel composed of 7 people.
Results and Discussion The results showed that the addition of dietary supplements of M. officinalis, O. decumbens vent and A. Vera L at the level of 0.5 or 2% ratio had no significant effect on LW 27d, LW 42d and feed conversion ratio of quail. However, the highest Carcass weight was recorded in group fed with A. Vera L-2% and the least Carcass weight was recorded in group fed with M. officinalis-2%. Qualitative factors, carcass components, microbial community, biochemical factors and meat pH significantly affected by levels of herbal plants powder (p≤0.05). M. officinalis-0.5% reduced cholesterol and triglyceride. We also recorded evidence that O.decumbens-2% Vent powders reduced the total bacterial and coliforms.  It was concluded that addition of 2% of A. vera to the diet can improve performance and addition of 2% of M. officinalis, O. decumbens a to the diet can improve meat shelf life in Japanese quails.
 

 

Keywords

Biochemical factors; Cholesterol; Japanese quail; Microbial count

References

1- Abbaspour, B., and S. D. Sharifi. 2015. A Study on the Antioxidants and Hypolipidemic Effects of Gracilariopsis Persica Seaweed in Japanese Quai. Research on Animal Production, 6(12): 12-19. (In Persian)

2- Amiri, H., H. Lari Yazdi, B. Dosti. and F. Samsamnia. 2011. Essential oil composition and anatomical study of Oliveria decumbens Vent. Iranian Journal of Medicinal and Aromatic Plants, 26(4): 513-520. (In Persian)

3- Bertram, H. C., A.K. Whittaker, W.R. Shorthose, H.J. Andersen, and A.H Karlsson. 2004. Water characteristics in cooked beef as influenced by ageing and high pressure treatment-an NMR micro imaging study. Meat Science, 66: 301-306.

4- Bouton, P.E., P.V.Harris. and S.Shorthose. 1971. Effect of Ultimate pH upon the Water-Holding Capacity and Tenderness of Mutton. Journal Of Food Science, 36: 435–39.

5- Darabighane, B., F.M. Aghjeh Gheshlagh, B. Navidshad, A. Mahdavi, A. Zarei, and S. Nahashon. 2017. Effects of Peppermint (Mentha Piperita) and Aloe Vera (Aloe Barbadensi) on Ileum Microflora Population and Growth Performance of Broiler Chickens in Comparison with Antibiotic Growth Promoter. Iranian Journal of Applied Animal Science, 7 (1): 101–8.

6- Donhof Ivan, E. 2000. Aloe vera, the wole leaf advantage. Journal of General Practice, 15: 112-124.

7- Habibi, H., N. Ghahtan and MA. kohanmoo. 2017. Effect of Dietary Supplementation of Three Powdered Melilotus officinalis, Oliveria decumbens Vent, Aloe vera L on Quantitative, Qualitative and Biochemical Properties of Japanese Quails’ Eggs. Livestock Research, 6 (12): 27-36. (In Persian).

8- Hajipour dehbalaei, Sh., M. Afsharmanesh, and M. Sami. 2016. Effect of essential oils of thyme, oregano and their combination on quality of quail meat in comparison with virginiamycin. Journal of Food Hygiene, 5(20): 45-54. (In Persian)

9- Irshad, S., M. Butt, and H. Younus. 2011. In-Vitro Antibacterial Activity of Aloe Barbadensis Miller (Aloe Vera). International Research Journal of Pharmaceutical, 1 (2): 59–64.

10- Jafarzadeh, A., H. Darmani Kuhi, N. Ghavi Hossein-Zadeh. and M. Roostaei-Ali Mehr. 2014. Effect of dietary Aloe vera gel powder supplementation on performance, lymphoid organ weights and immune response of Japanese quails. Animal Production Research, 3(1): 31-41. (In Persian).

11- Jebelli Javan, A., M. Saberi, A. Javaheri Vayeghan, S. Ghaffari Khaligh, H. Rezaian and N. Nejabat. 2013. The effect of dietary Aloe vera gel extract supplementation on lipid peroxidation of broiler breast fillets during frozen storage. Journal of Veterinary Research, 68 (3): 233-240.

12- Klaver, F.A.M. and R.V.D. Meer. 1993. The Assumed Assimilation of Cholesterol by Lactobacilli and Bifidobacterium Bifidum Is Due to Their Bile Salt-Deconjugating Activity. Applied And Environmental Microbiolog, 59 (4): 1120–24.

13- Khosravinezhad. M., E. Talebi, Shivakumar, Z. Nemati. and I. Nasrollahi. 2017. Essential Oil Composition and Antimicrobial , Antioxidant Activities of Oliveria Decumbens Vent. International Journal of Herbal Medicine, 5 (2): 102–6.

14- Lin, D. and M.T. Morrissey. 2014. Iced Storage Characteristics of Northern Squawfish (Ptychocheilus Oregonensis). Journal of Aquatic Food Product Technology, 8: 37–41.

15- Luna, A., M. C Labaque, J. A. Zygadlo. and R. H. Marin. 2010. Effects of thymol and carvacrol feed supplementation on lipid oxidation in broiler meat. Poultry Science, 89: 366-370.

16- Martino, E., I. Ramaiola, M. Urbano, F. Bracco. and S. Collina. 2006. Microwave-Assisted Extraction of Coumarin and Related Compounds from Melilotus Officinalis (L.) Pallas as an Alternative to Soxhlet and Ultrasound-Assisted Extraction. Journal of Chromatography, 1125: 147–51.

17- Mahboubi, M., M. M. Feizabadi, G. Haghi. and H. Hosseini. 2008. Antimicrobial activity and chemical composition of essential oil from Oliveria decumbens Vent. Iranian Journal of Medicinal and Aromatic Plants, 24(1): 56-65. (In Persian)

18- Plesca-Manea, L., A. E. Parvu, M. Parvu, M. Taamas, R. Buia, and M. Puia. 2002. Effects of Melilotus Officinalis on Acute Inflammation. Phytotherapy Research, 16: 316–19.

19- Quijano-Celis, C. E., A. P. Jorge. and M. Gustavo. 2010. Chemical Composition of the Leaves Essential Oil of Melilotus Officinalis (L.) Pallas from Colombia. Journal of Essential Oil Bearing Plants, 13 (3): 313–15.

20- Rouzmehr, F., Y. Chashnidel, M. Rezaei, M. Mohiti Asli, and M. Mottaghi Talab. 2017. The Effect of Thyme and Cinnamon Microencapsulated Essential Oils on Performance, Some Blood Parameters and Carcass Characteristic in Boiler Chicks. Research on Animal Production, 8(17): 34-42. (In Persian)

21- Sahu, P. K., D. Dayal Giri, R. Singh, P. Pandey, S. Gupta, A. K. Shrivastava, A. Kumar, and K. D. Pandey. 2013. Therapeutic and Medicinal Uses of Aloe Vera : A Review. Pharmacology & Pharmacy, 4: 599–610.

22- Sedaghat, A., M. A. Karimi Torshizi, and Sh, Rahimi. 2016. Effect of different levels of camphor on performance and serum concentration of thyroid hormones and testosterone in Japanese quail. Animal Production, 18(2): 273-286. (In Persian)

23- Shaeri, M., A. Mohti, Z. Ansari Pirsaraei, and M. Taghizadeh. 2012. The Effect of Anethum graveolensEssential Oil on Some Blood Parameters, Egg Yolk Cholesterol Concentration, Hatchability and Chick Quality in Broiler Breeder Hens. Research on Animal Production, 3 (6): 15-24. (In Persian)

24- Suganya, T., S, Senthilkumar, K. Deepa, J. Muralidharan, G. Gomathi, and S. Gobiraju. 2016. Herbal feed additives in poultry. International Journal of Science, Environment and Technology, 5 (3): 1137–45.

25- Talazadeh, F., M. Mayahi, S. M. Jalali, and V. Asgari. 2016. The effect of thyme extract (Thymus vulgaris) on feed intake, weight gain, feed conversion ratio and blood serum lipids profile of broiler chickens. Iranian Veterinary Journal, 12(3): 51-57. (In Persian)

26- Thiruppathi, S., V. Ramasubramanian, T. Sivakumar, and V. Thirumalai arasu. 2010. Antimicrobial Activity of Aloe Vera (L.) Burm. F. against Pathogenic Microorganisms. Journal Of Biosciences Research, 1 (4): 251–58.

27- Turner, T. T, and J. J. Lysiak. 2008. Oxidative stress: A common factor in testicular dysfunction. Journal of Andrology, 29: 488-498.

28- Van Riper, C. L, and D.L. Larson. 2009. Role of Invasive Melilotus Officinalis in Two Native Plant Communities Communities. Plant Ecology, 200: 129–139.

29- Windisch, W., K. Schedle, C. Plitzner, and A. Kroismayr. 2014. Use of Phytogenic Products as Feed Additives for Swine and Poultry. Journal of Animal Science, 86: 140–48.

30- Yazadani, S., Z. Ansari Pirsaraei, H. Deldar, S. A. Jafarpour, and S. Beheshti Moghadam. 2015. Effect of different levels of Aloe vera on performance, carcass characteristics, meat quality and blood paramerers of broiler chicks. Animal Science Journal (Pajouhesh & Sazandegi), 110: 67-80.

31- Yazadani, S., Z. Ansari Pirsaraei, H. Deldar, and S. A. Jafarpour. 2015. Effect of different levels of Aloe Vera gel and neomycin on performance, some carcass characteristics, and physical and chemical meat quality of broiler chicks. Poultry Science Researche. 2 (1): 9-20.

32- Yingyuad, S., S. Ruamsin, D. Reekprkhon, S. Reekprkhon, S. Pongamphai, and U. Siripatrawan. 2006. Effect of Chitosan Coating and Vacuum Packaging on the Quality of Refrigerated and Science. Packaging Technology and Science, 19: 149–57.