Chemical Composition and Ruminal Dry Matter and Protein Degradation Parameters of Canola Meal Treated by Microwave Irradiation

پژوهشهای علوم دامی ایران

Article 3, Volume 12, Issue 3 - Serial Number 43, September 2021, Pages 291-305   

Document Type: Ruminant Nutrition

DOI: 10.22067/ijasr.v12i3.77304

Authors

Seyed Rohollah Ebrahimi Mahmood Abad^{* 1}; Ali Nikkhah²; Ali asgar Sadeghi³

¹Department of Animal Science, Faculty of Agriculture, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

²Department of Animal Science, Faculty of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Tehran, Iran

³Department of Animal Science, Faculty of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Tehran, Iran.

Abstract

Introduction: In growing ruminants or early lactation dairy cows, production may be limited by a dietary metabolizable protein. In these conditions microbial protein synthesis is not sufficient to meet the animal’s protein requirement. Canola meal (CM), which is an available and good source of protein in ruminant nutrition, especially in Iran. However the protein of CM is highly degradable by rumen microorganisms and on the other hand, anti-nutritional factors, such as phytic acid and glucosinolate in brassica-originated feed, are of concern relative to animal-originated feeds. Glucosinolates are a large group of sulphur-containing secondary plant metabolites and are known to reduce feed intake, induce iodine deficiency and depress fertility in ruminants. Several heat processing methods have been used to enhance nutritive value of oilseed meals, including extrusion, roasting, toasting and Jet-Sploding. Recently, treatment of oilseed meals with microwave irradiation was successful in reducing ruminal degradable protein, anti-nutritional factors and increasing digestible undegradable protein of them. Microwave irradiation is heating faster, processing in less time and higher energy efficiency compared to conventional methods. This research was carried out to evaluate the effects of microwave irradiation (800 W) for 2, 4 and 6 min on dry matter (DM), crude protein (CP) and true protein (TP) ruminal degradability, in vitro CP digestibility, antinutritional factors (glucosinolate and phytic acid), and chemical composition of CM.
 
Materials and Methods: The DM of CM was determined and then, sufficient water was added to sample to increase the moisture content of CM to 250 g/kg. Three samples (500 g each) were subjected to microwave irradiation at a power of 800 W for 2, 4 and 6 min. The samples were ground to pass a 2 mm screen for the ruminal in situ study. Degradation kinetics of DM, CP and TP were determined according to in situ procedure. Six grams of untreated or irradiated feed samples were incubated in the rumen of three ruminally fistulated Taleshi bulls for periods of 0, 2, 4, 8, 16, 24 and 48 h. The bulls were fed with a total mixed ration containing 700 g/kg of DM forage (700 g/kg alfalfa hay and 300 g/kg wheat straw on DM basis) and 300 g/kg of DM concentrate. The concentrate consisted of ground barley grain, canola meal, ground canola seed, cotton-seed meal, wheat bran, dicalcium phosphate and a vitamin+mineral premix (530, 130, 160, 40, 120, 10 and 10 g/kg DM, respectively). TP of samples were determined by Bradford^,s procedure. Digestibility of rumen undegraded CP was estimated using the three-step in vitro procedure. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to monitor protein subfractions and the fate of true proteins of untreated and irradiated feed samples in the rumen.
 
Results and Discussion: Microwave irradiation had no effect on improving chemical composition of CM but decreased the total glucosinolate and phytic acid of CM linearly and quadratically. Microwave irradiation for 2, 4 and 6 min decreased the phytic acid content of CM by 13, 30 and 51% respectively, compared to untreated CM. The total glucosinolate contents of CM microwave irradiated for 2, 4 and 6 min decreased by 38.4, 56.0 and 67.1% respectively, compared to untreated samples. Microwave irradiation decreased the washout fraction, degradation rate and effective degradability (ED) of DM, CP and TP and increased potentially degradable fraction of DM, CP and TP of CM. The washout fraction of CP decreased by 15.7 and 39.8% in samples irradiated for 2, 4 and 6 min, respectively. Irradiation for 2, 4 and 6 min decreased ED of CP at a ruminal outflow rate of 0.05 h^-1 by 13.6 and 22.7%, respectively. In vitro CP digestibility of CM increased by treating with microwave irradiation up to 4 min. CP digestibility of 2 and 4 min irradiated CM was increased by 6.9 and 10.5%, respectively. Electrophoresis results also indicated that major proteins of CM was Cruciferin (globulin 12S) and Napin (Albumin 2S). Electrophoresis results indicated that in untreated CM, four subunits of Cruciferin and in microwave irradiated CM, four subunits of cruciferin and two subunits of Napin consisted of by-pass proteins.
 
Conclusion: In this study, microwave irradiation, reduced ruminal degradability of CP and TP, increased in vitro CP digestibility and reduced anti-nutritional factors of CM. Subsequently, in vivo studies are required to investigate effect of feeding irradiated feedstuff on lactation performance of dairy cows.
 

 
 

Keywords

Anti-nutritional factors; Canola Meal; Degradability; Irradiation

References

-Ahmadi, K., H. R., Ebadzadah, H., Abshah, A. Kazemian, and M. Rafie. 2018. Statistics of Agriculture Agricultural year, 2016-2018. Ministry of Agriculture, Deputy of Planning and Economics, Information and Communication Technology Center. (In Persian).

2-Ahlin, K. A., M., Emmanuelson, and H. Wiktorsson. 1994. Rapeseed products from double cultivars as feed for dairy cow: effects of long term feeding on thyroid function, fertility and animal health. Acta Veterinaria Scandinaviva. 35: 37-53.

3-Alajaji, S. A., and T. A. El-Adawy. 2006. Nutritional composition of chickpea (Cicer arietinum L.) as affected by microwave cooking and other traditional cooking methods. Journal of Food Composition and Analysis, 19: 806–812.

4-Al-Kaiesy, M. T., H. A. Abdul-Kader, M. H. Mohammad, and A. H. Saeed. 2003. Effect of gamma irradiation on antinutritional factors in broad bean. Radiation. Physics and Chemistry, 67: 493-496.

5-AOAC. 1995. Official Methods of Analysis, 16^th Ed. Association of Official Analytical Chemists. Arlington, VA, USA.

6-Banik S., S., Bandyopadhyay, and S. Ganguly. 2003. Bio-effects of microwave. Bioresource Technology. 87: 155 –159.

7-Bhatty R. S., S. L., McKenzie and A.J. Finlayson. 1999. The proteins of rapeseed soluble in salt solutions. Canadian Journal of Biochemistry. 46: 1191-1197.

8-Bjarnason, J., and K. J. Carpenter. 1969. Mechanisms of heat damage in protein. I. Model with acetylated lysine units. British Journal of Nutrition. 23:858-868.

9-Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72: 248-255.

10-Calsamiglia, S., and M. D. Stern. 1995. A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants. Journal of Animal Science. 73: 1459-1465.

11-Chen, Q. C., and W. L. Betty. 2003. Separation of phytic acid and other related inositol phosphates by high-performance ion chromatography and its applications. Journal of Chromatography A, 1018: 41-52.

12-Clifford, A., and D. V. Smith. 1987. Rapid method for determinig total glucosinolates in rapeseed by enzimatically released glucose. Journal of the Science of Food and Agriculture, 38: 141-150.

13-Deacon, M.A., G., de Boer, and J. J. Kennelly, 1988. Influence of jet-sploding and extrusion on ruminal and intestinal disappearance of canola and soybeans. Journal of Dairy Science, 71: 745-753.

14-De Boland, A. R., G. B. Garner, and B. L. O Dell. 1975. Identification and properties of phytate in cereal grains and oilseed products. Journal of Agriculture and Food Chemistry, 23: 1186- 1189.

15-Ebrahimi Mahmoudabad, S. R., A., Nikkhah, and A. A. Sadeghi. 2016. Aninutritional factors and ruminal degradation of dry matter and crude protein of microwave and gamma irradiated native rapeseed. Iranian Journal of Animal Science Research. 8 (1): 72-85. (In Persian).

16-Ebrahimi, S. R., A., Nikkhah, and A. A.Sadeghi. 2010. Changes in nutritive value and digestion kinetics of canola seed due to microwave irradiation. Asian-Australasian Journal of Animal Science, 27: 347-354.

17-Fakhouri, M. O., and H. S. Ramaswamy. 1993. Temperature uniformity of microwave heated food as influenced byproduct type and composition. Food Research International, 26: 89-95.

18-Fathi Nasri, M. H., J., France, M. Danesh Mesgaran, and E. Kebreab. 2008. Effect of heat processing on ruminal degradability and intestinal disappearance of nitrogen and amino acids in Iranian whole soybean. Livestock Science, 113: 43 –51.

19-Fenwick G. R., E. A., Spinks A. P., Wilkinson, R. K., Heaney, and M. A. Legoy. 1986. Effect of processing on the antinutrient content of rapeseed. Journal of the Science of Food and Agriculture, 37: 735–741.

20-Finley, J. W. 1989. Effects of processing on proteins: an overview. In: Protein Quality and the Effects of Processing (Ed. R.D. Phillips and J.W. Finley) Marcel Dekker, New York, NY, USA, pp. 1-7.

21-Folawiyo, Y. L., and R. K. O. Apenten. 1997. The effect of heat and acid treatment on the structure of rapeseed albumin (napin). Food Chemistry, 58: 237-243.

22-Ghanbari, F., T., Ghorchi, P., Shawrang, H., Mansouri, and N. Torbatinejad. 2014. Effect of irradiation on runinal disappearance of dry matter and crude protein and in vitro digestibility of canola meal. Journal of Animal Science (pajouhesh an sazandegi), 26: 55-66. (In Persian).

23-Gharaghani, H., M., Zaghri, G., Shahhosseini, and H. Moravej. 2008. Effect of gamma irradiation on anti-nutritional factors and nutrition value of canola meal for broiler chickens. Asian-Australasian Journal of Animal Science, 21: 1479-1485.

24-Huang, S., M., Liang, G., Lardy, H. E., Huff, M. S., Kerley, and F. Hsieh. 1995. Extrusion process of rapeseed meal for reducing glucosinolates. Animal Feed Science and Technology, 56: 1-9.

25-Khorasani, G. R., P. H., Robinson, and J. J. Kennelly. 1989. Effects of chemical treatment on in vitro and in situ degradation of canola meal crude protein. Journal of  Dairy Science, 72, 2074-2080.

26-Jalilian, S., F., Fattahnia, P., Shawrang, and H. Mohammadzadah. 2015. Effect of different irradiation on runinal degradability and intestinal crude protein digestibility of sunflower meal. Journal of Animal Science Research, 25 (2). 69-80. (In Persian).

27-Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature, 227: 680-685.

28-Maheshwari, P. N., D. W., Stanley, and F. R. Van der Van. 1980. Microwave treatment of dehulled rapeseed meal to inactivate myrosinase and its effect on oil meal quality. Journal of American Oil Chemistry and Society. 57: 194–199.

29-Mandiki, S. N. M., G., Derycke, J. L., Bister, N., Mabon, J. P., Wathelet, M., Marlier, and R. Paquay, 2002. Chemical changes and influence of rapeseed antinutritional factor on gestating and lactating ewes. Part 1. Animal performances and plasma hormones and glucose. Animal Feed Science and Technology, 98: 25–35.

30-McNeill, L., K., Bernard, and M. G. MacLeod. 2004. Food intake, growth rate, food conversion and food choice in broilers fed on diets high in rapeseed meal and pea meal with observations of the resulting poultry meat. British Poultry Science, 45: 519–523.

31-Michalet-Doreau, B., and M.Y. Ould-Bah. 1992. In vitro and in sacco methods for the estimation of dietary nitrogen degradability in the rumen: a review. Animal Feed Science and Technology, 40: 57-86.

32-Moshtaghi Nia, S.A., and J. R. Ingalls. 1995. Influence of moist heat treatment on ruminal and intestinal disappearance of amino acids from canola meal. Journal of Dairy Sci. 78: 1552-1560.

33-Mubarak, A.E. 2005. Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chemistry. 89: 489-495.

34-Murray, R.K., D.K., Granner, P.A., Mayes, and V.W. Rodwell. 2003. Harper’s Biochemistry, 26^th Ed. McGraw-Hill, New York, NY, USA.

35-National Research Council, 2001. Nutrient Requirements of Dairy Cattle. 7^th Ed. National Academy of Sciences, Washington, DC. Ananymous.

36-Oerlemans, K., D. M., Barrett, C.B., Suades, R. Verkerk, and M. Dekker. 2006. Thermal degradation of glucosinolates in red cabbage. Food Chemistry, 95: 19–29.

37-Oliveira, M. E. C., and. A. S. Franca. 2002. Microwave heating of foodstuffs. Journal of Food Engineering, 53: 347-359.

38-Ørskov, E. R., and I. McDonald. 1979. The estimation of protein degradability in the rumen from incubation weighed according to rate of passage. Journal of Agriculture Science (Camb.), 92: 499-503.

39-Paya, H., A., Taghizadah, M., Janmohammadi, G. A., Moghaddam, and A. HosseinKhani. 2016. Effect of microwave irradiation on ruminal and intestinal disapearance of dry matter and crude protein of safflower seed by I situ and mobile nylon bag techniques. Journal of Animal Science Research, 26 (2): 121-130. (In Persian).

40-Peng Q., N.A., Khan, Z., Wanng, and D. Moist. 2014. Dry heating induced changes in protein molecular stracture, protein sub fractions and nutrient profiles in camelina seed. Journal of Dairy Science, 97: 446-457.

41-PHOTO-CAPT. 1999. V. 99. B.P. 66 TORCY. http://www.vilber.com

42-Piradl, A., R. Mirmohammadi, and H. Khalilvandi Behroozyar. 2017. Investigation of chemical changes, ruminal degradation and ruminal digestion of starch and crude protein of different variety of microwave irradiated barley grains. Journal of Ruminants Research, 5 (4): 119-144. (In Persian).

43-Sadeghi, A.A. and P. Shawrang, 2006. Effects of microwave irradiation on ruminal degradability and in vitro digestibility of canola meal. Animal Feed Science and Technology. 127: 45-54.

44-Sadeghi, A.A. and P. Shawrang. 2007. Effects of microwave irradiation on ruminal protein degradation and intestinal digestibility of cottonseed meal. Livestock Science. 106: 176–181.

     45- SAS. 1996. Statistical Analysis System. SAS Intit. Inc., Cary, NC, USA.

46-Shawrang, P., S., Jalilian, F., Fattahnia, A. A., Sadeghi, and A.A. Mehrabi. 2017. Effect of irradiation from gamma, electron beam, microwave and infrared sources on ruminal degradability and in vitro digestibility of soybean meal. Journal of Animal Science Research, 27 (4). 217-230. (In Persian).

47-Sheikhalipoor, A., A., Hosseeinkhani, A., Taghizadah, and H. Mohammadzadah. 2018. Effect of different heat processing methods on nutritive value of common vetch seed in ruminants. Iranian Journal of Animal Science, 49: 427-436. (In Persian)

48-Siddhuraju, P., H. P. S. Makkar, and K. Becker. 2002. The effect of ionising radiation on anti-nutritional factors and the nutritional value of plant materials with reference to human and animal food. Food Chemistry, 78: 187-205.

49-Standford, K., G. L., Wallins, W. G., Smart, and A.A. Mc Allister. 2000. Effect of feeding canola screenings on apparent digestibility, growth performance and carcass characteristics of feedlot lambs. Canadian Journal of Animal Science, 80: 355-365.

50-Stefanello, C., S. L., Vieira, G. O., Santiago, L., Kindlein, J. B. O., Sorbara, and A. J. Cowieson. 2015. Starch digestibility, energy utilization, and growth performance of broilers fed corn-soybean basal diets supplemented with enzymes. Poultry Science, 94: 2472-2479.

51-Tamminga, S., W. M., Van Straalen, A. P. J., Subnel, R. G. M., Meijer, A., Steg, C.J.G., Wever, and M. C. Blok. 1994. The Dutch protein evaluation system: the DVE/OEB-system. Livestock Production Science, 40: 139–155.

52-Utsumi, S., and J. E. Kinsella. 1985. Structure function relationship in food proteins: Subunit interactions in heat induced gelation of 7S, 11S and soy isolate proteins. Journal of Agriculture and Food Chemistry. 33: 297–303.

53-Vallejo, F., F. A., Tomas-Barbern, and C. Garcia-Viguera. 2002. Glucosinolate and vitamin C contents in edible parts of broccoli florets after domestic cooking. European Food Research and Technology. 215: 310-316.

54-Van Soest, P. 1989. A reanalysis the digestibility of bound N in distillers grains. Proc. Cornell Nutr. Conf. Syracuse, NY. 127-136.

55-Van Soest, P. J. 1994. Nutritional Ecology of the Ruminants. 2nd Edition. Cornell University Press. NY. USA.

56-Van Soest, P. J., J. B., Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74: 3583–3597.

57-Wagner, J. R., D. A., Sorgentini, and M. C. Anon. 2000. Relation between solubility and surface aromatic hydrophobicity as an indicator of modification during preparation processes of commercial and laboratory prepared soy protein isolates. Journal of Agriculture Food Chemistry, 48: 3159–3165.

58-Waldern, D. E. 1973. Rapeseed meal versus soybean meal as the only protein supplement for lactating cows fed corn silage roughage. Canadian Journalof Animal Science. 53: 107-112.

59-Wright, C.F., M. A. G., Von Keyserlingk, M. L., Swift, L. J., Fisher, J. A., Shelford, and N. E. Dinn. 2005. Heat and Lignosulfonate treated canola meal as a source of ruminal undegradable protein for lactating dairy cows. Journal of. Dairy Science. 88: 238-243.

60-Yan, X., N. A., Khan, F., Zhang, L., Yang, and P. Yu. 2014. Microwave irradiation induced changes in protein molecular structures of barley grains: relationship to changes in protein chemical profile, protein subfractions, and digestion in dairy cows. Journal of Agriculture and Food Chemistry, 62 (28): 6546-6555.

61-Zeb, A. 1998. Possibilities and limitations of feeding rapeseed meal to broiler chicks. Ph.D thesis., Georg-August University Gottingen, Germany.