Allelopathic Effects of Sorghum (Sorghum bicolor L.) and Russian Knapweed (Acroptilon repens L.) Aqueous Extract on Seed Germination Indices and Enzyme Activity of Some Field Crops and Weeds

پژوهش های حفاظت گیاهان ایران

Article 9, Volume 31, Issue 4 - Serial Number 38, December 2018, Pages 676-689   

Document Type: Research Article

DOI: 10.22067/jpp.v31i4.62227

Authors

azam hatami hampa¹; abdollah javanmard^{* 2}; mohammad taghi elebrahim³; omid sofalian³

¹matagheh university

²Maragheh university

³University of Mohaghegh Ardabili

Abstract

Introduction: The loss of crop yield due to weeds is enormous. Potential yield reductions caused by uncontrolled weed growth throughout a growing season have an estimated range of 45-95%, depending on ecological and climatic conditions. Overusing synthetic herbicides may affect the environmental, human health and food. Furthermore, increasing use of herbicides has resulted in a dramatic increment in the herbicide resistance among weeds, and over 307 weed resistant biotype belonging to 183 species (110 dicots and 73 monocots) have been identified worldwide. Cultivating crops with allelopathic potential can reduce the dependency on synthetic herbicides and increase crop yields. Sorghum (Sorghum bicolor L.) has been reported as one of the most allelopathic crops extensively used as cover and smother crops and also incorporated in the soil for weed suppression. Moreover, Russian knapweed (Acroptilon repens L.) has been shown to produce phytotoxic compounds and plant growth inhibitors, which may contribute to its competitive behavior. The phenomenon of allelopathy has been suggested to be one of the possible alternatives for achieving sustainable weed management.
Materials and Methods: In order to evaluate the allelopathic effects of sorghum (Sorghum bicolor L.) and Russian knapweed (Acroptilon repens L.) extract on seed germination indices and enzymatic activity of wheat (Triticum aestivum L.), sugar beet (Beta vulgaris L.), common lambsquarters (Chenopodium album L.) and redroot pigweed (Amaranthus retroflexus L.), an experiment was conducted as factorial experiment based on randomized completely design with three replications in Faculty of Agriculture, University of Maragheh in 2016. The investigated factors were four crops (wheat, sugar beet, common lambsquarters and redroot pigweed), shoot aqueous extract (sorghum and Russian Knapweed) and aqueous extract concentrations at 0 (control), 5, 10 and 20% (m/v). Sorghum and Russian knapweed crops were collected during July 2016. The plants were taken to the laboratory and kept fresh in a refrigerator. In addition, Kato-Noguchi et al., (17) method was followed for extraction. Samples of the above-ground tissues (stems and leaves) of sorghum and Russian knapweed were washed thoroughly with tap water and rinsed with distilled water. They were clipped and then passed through a 1 mm screen before storage in a refrigerator at 2 ℃. Each 50, 100 and 200 g samples was extracted by soaking it in 1 L of distilled water at 24 ℃ during 24 h in a shaker. All extracts were filtered through two layers of cheese cloth to remove fibers. Distilled water was also considered as the control treatment.
Results and Discussion: Results indicated that percentage and rate of germination, radicle and plumule length, radicle dry weight, plumule dry weight, seedling dry weight and vigor length index of all crops decreased significantly by increasing aqueous extract concentration of sorghum and knapweed. These results are in agreement with those previously reported that the degree of inhibition increased by increasing extract concentration. Using Russian knapweed aqueous extract, germination percentage was 12.62 percent greater than sorghum aqueous extract. Moreover, inhibitory effect of sorghum aqueous extract on germination rate loss was higher than that of aqueous extract of Russian knapweed. As to field crops, inhibitory effect of sorghum and Russian knapweed extract on sugar beet was higher than wheat. Moreover, the effect of sorghum and knapweed extract on radicle, plumule and seedling fresh weight loss of redroot pigweed and common lambsquarters (weeds) was greater as compared with that on field crops (wheat and sugar beet). Increasing concentration of sorghum extract decreased seed vigor indices of common lambsquarters and redroot pigweed by 83.36 and 87.15% relative to control, respectively. Activity of peroxidase and polyphenoloxidase enzymes was reduced and increased, respectively, as a result of an increment in extract concentration. Batish et al. (7) have demonstrated that parthenin (Parthenium hysterophorus) impairs mung bean growth by affecting respiration, protein content and activities of protease and peroxidase enzymes. The minimum value of plumule and seedling length was observed for redroot pigweed and common lambsquarters weeds with application of 20% aqueous extract of sorghum and Russian knapweed. The values of weed tolerance index (WTI) revealed that redroot pigweed and common lambsquarters were more sensitive to aqueous extract of sorghum and Russian knapweed. The WTI combines several individual measured parameters and ranges between 0 and 1. In this case, a low WTI indicates a high susceptibility to biotic or abiotic stress originated from crop extracts.
Conclusions: Our results showed that aqueous extracts of sorghum and Russian knapweed had an inhibitory effect on seed germination indices of wheat, sugar beet, common lambsquarters and redroot pigweed. It can be also concluded that sorghum and Russian knapweed possess weed-suppressing ability and seem to be useful for developing natural herbicides.

Keywords

Common lambsquarters; Polyphenoloxidase; Redroot Pigweed; Seed vigor; Weed tolerance index

References

1- Abdul-Baki A.A., and Anderson J.D. 1973. Vigor determination in soybean by multiple criteria. Crop Science, 13: 630-633.

2- Alford E.R., Perry L.G., Qin B.O., Vivanco J.M., and Paschke M.V. 2007. Aputative allolopathic agent of Russian knapweed occurs in invaded soild. Soil Biology and Biochemistry, 39: 1812-1815.

3- Algandaby M.M., and El-Darier S.M. 2016. Management of the noxious weed; Medicago polymorpha L. via allelopathy of some medicinal plants from Taif region, Saudi Arabia. Saudi Journal of Biological Sciences. Accepted 8 February 2016, Available online 12 February 2016.

4- Alsaadawi1 I.S., Al-Khateeb T.A., Hadwan H.A., and Lahmood N.R. 2015. A chemical basis for differential allelopathic potential of root exudates of Sorghum bicolor L. (Moench) cultivars on companion weeds. Journal of Allelochemical interactions, 1 (1): 49-55.

5- Amini S., Azizi M., and Joharchi M.R. 2014. Determination of allelopathic potential in some medicinal and wild plant species of Iran by dish pack method. Theoretical and Experimental Plant Physiology, 26 (2):189-199.

6- Azirak S., and Karaman S. 2008. Allelopathic effect of some essential oils and components on germination of weed species. Acta Agriculturae Scandinavica, 58: 88-92.

7- Batish D.R., Singh H.P., Kohli R.K., Saxena D.B., and Kaur S. 2002. Allelopathic effects of parthenin against two weedy species, Avena fatua and Bidens pilosa. Environmental and Experimental Botany, 47:149–155.

8- Chon S.U., Jang H.G., Kim D.K., Kim Y.M., Boo H.O., and Kim Y.J. 2005. Allelopathic potential in lettuce (Lactuca Sativa L.) plants. Scientia Horticulture, 106 (3): 309-317.

9- El-Khatib A.A., Hegazy A.K., and Gala H.K. 2004. Does allelopathy have a role in the ecology of Chenopodium murale? Annales Botanici Fennici, 41:37-45.

10- Farhoudi R., and Lee D. 2013 Allelopatic Effects of Barley Extract (Hordeum vulgare) on sucrose synthase activity, lipid peroxidation and antioxidant enzymatic activities of hordeum spontoneum and avena ludoviciana. Proceedings of the National Academy of Science, 83:447–452.

11- Fateh E., Sohrabi S.S., and Gerami F. 2012. Evaluation of the allelopathic effect of bindweed (Convolvulus arvensis L.) on germination and seedling growth of millet and basil. Advances in Environmental Biology, 6(3): 940-950.

12- Gharanjic A., Gholamalipour Alamdari E., Biabani A., and Haghighi Haghighi A. 2014. Evaluating the allelopathic potential of (Polygonum convolvulus L.) on wheat (Triticum aestivum L.). Applied Research of Plant Ecophysiology, 1 (1): 83-95. (In Persian with English abstract)

13- Han C.M., Pan K.W., Wu N., Wang J.C., and Li W. 2008. Allelopathic effect of ginger on seed germination and seedling growth of soybean and chive. Scientia Horticulturae, 116: 330-336.

14- Hartman H., Kester D., and Davis F. 1990. Plant propagation, principle and practices. Prentice Hall Imitational Edition, 647Pp.

15- Jabran k., Mahajan G., Sardana V., and Chauhan B.S. 2015. Allelopathy for weed control in agricultural systems. Crop Protection, 72: 57-65.

16- Kang G.Q., Wan F.H., Liu X., and Guo L. 2008 Influence of two allelochemicals from Ageratina adenophora Sprengel on ABA, IAA and ZR contents in roots of upland rice seedlings. Allelopathy Journal, 21: 253-262.

17- Kato-Noguchi H., and Macias F.A. 2008. Inhibition of germination and α-amylase induction by 6-methoxy-2-benzoxazolinone in twelve plant species. Biologia Plantarum, 52 (2): 351-354.

18- Khaliq A., Matloob A., Mahmood S., and Wahid A. 2013. Seed pre-treatments help improve maize performance under sorghum allopathic stress. Journal of Crop Improvement, 27(5): 586-605.

19- Macias F.A., Molinill R.M., Varela J.C.G., and Galindo J. 2007. Allelopathy a natural alternative for weed control. Pest Management Science. 63:327-348.

20- Mandel M.S.H., Masum S.M., Ali M.H., Haque M.N., and Mahto A.K. 2012. Influence of parthenium hysterophorus, chromolaena odorata and PRH on seed germination and seedling growth of maize, soybean and cotton. Bangladesh Journal of Weed Science, 3 (1&2): 83-90.

21- Mojab M., and Mahmoodi S. 2007. Allelopathic effects of shoot and root water extracts of Hoary cress (Cardaria draba) on germination characteristic and seedling growth of Sorghum (Sorghum bicolor L.). Electronic Journal of Crop Production, 1(4):65-78. (In Persian with English abstract).

22- Naderi R., Yazdani A., Emam Y., and Bijanzadeh E. 2015. Allelopathic effects of medicinal plant madder (Rubia tinctorum) on germination characteristics and seedling growth of sorghum (Sorghum bicolor), corn (Zea mays), field bindweed (Convolvolus arvense) and Johnsongrass (Sorghum halepense). Plant Protection Journal, 6 (4): 395-407.

23- Oracz K., Bailly C., Gniazdowska A., Côme D., Corbineau D., and Bogatek R. 2007 Induction of oxidative stress by sunflower phytotoxins in germinating mustard seeds. Journal of Chemistry Ecology, 33:251–264.

24- Peerzada A.M., Haider A., Singh H., and Chauhan B. 2016. Weed management in sorghum (Sorghum bicolor L. Moench) using crop competition. Crop Protection, 1-7.

25- Pirzad A., Ghasemian V., Darvishzahed R., Sedghi M., Hassani A., and Onofri A. 2010. Allelopathy of sage and white wormwood on purslane germination and seedling growth. Notulae Scientia Biologicae 2(3):91-95.

26- Porheidar Ghafarbi S., Eslami S.V., Hassannejad S., Alizade H., and Zamani G. 2012. Allelopathic effects of rye (Secale cereal L.) on corn (Zea maize L.) and some of its important weeds. Journal of sustainable Agriculture and Production science, 1: 149-163. (In Persian with English abstract).

27- Qian H.X.X., Chen W., Jiang H., Yuanxiang J.Y., Weiping L.W., and Fu Z .2009. Allelochemical stress causes oxidative damage and inhibition of photosynthesis in Chlorella vulgaris. Chemosphere, 75(3): 368-375.

28- Quintana N., Weir T.L., Du J., Brockling C.D., Rieder J.P., Stermitz F.R., Pasckke M.W., and Vivanco J.M., 2008. Phytotoxic polyacetylenes from roots of Russian Knapweed (Acroptilone repens L.). Phytochemistry, 69: 2572–2578.

29- Rasam G.H., Torabi B., Garossi F., and Badri A. 2015. Allolopathic effect of ziziphora (Ziziphora clinopodioidis Lam) on germination and seedling growth of wheat cultivars. Journal of Seed Science and Technology, 5 (14): 68-77. (In Persian with English abstract).

30- Rojas E.G., Silva M.P.O., Magenta M.A.G., and Thoma W. 2012. Investigation of phenolic compounds with allelopathic potential in leaves of a tree invader of restinga (Terminalia catappa L.). Unisanta Biology Science, 1(2): 60-64.

31- Roohi A., Tajbakhsh M., Saeidi M.R., and Nikzad P. 2009. Study the allelopathic effects of walnut (Juglans regia) water leaf extract on germination charactristics of wheat (Triticum astivum), onion (Allium cepa L.) and Lactuca (Lactuca sativa L.). Iranian Journal of Field Crops Research, 7(2): 457-464. (In Persian with English abstract).

32- Safari H., Tavili A., and Saberi M. 2010. Allelopathic effects of Thymus kotschyanus on seed germination and initial growth of Bromus tomentellus and Trifolium repens. Frontiers of agriculture in china, 4(4): 475–480.

33- Shang Z.H., and Xu S.G. 2012. Allelopathic testing of pedicularis kansuensis (Scrophulariaceae) on seed germination and seedling growth of two native grasses in the Tibetan plateau. International Journal of Experimental Botany, 81: 75-79.

34- Shehata H.F. 2014. Allelopathic potential of Portulaca oleracea L. seed extracts on germination and seedling growth of Cichorium endivia L., Lactua sativa L., Echinochloa crus-galli L., and Brassica tournefortii Gouan. Journal of Experimental Biology, 2(4): 388-396.

35- Sturm D.J., Kunz C., and Grehards R. 2016. Inhibitory effects of cover mulch on germination and growth of Stellaria media (L.) Vill. Chenopodium album L. and Matricaria chamomilla L. Crop Protection, 90: 121-130.

36- Tahamizarandi M.K., and Rezvanimoghadam P. 2011. Investigation of germination and seedling morphological characteristics of wild oat (Avena ludoviciana) under aqueous extract of the aerial parts of medicinal plants. Crop Protection, 25: 398-406.

37- Tigre R.C., Silva N.H., Santos M.G., Honda N.K., falcao E.P.S., and Pereira EC. 2012. Allelopathic and bioherbicidal potential of cladonia verticillaris on the germination and growth of lactuca sativa. Ecotoxicology and Environmental safety, 84: 125 – 132.

38- Yang C.M., Chang F., Li S.J., and Chou C.H. 2004. Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedlings: II. Stimulation of consumption-orientation. Botanical Bulletin of Academia Sinica, 45: 119-125.

39- Yang G., Wan F., Liu W., and Guo J. 2008. Influence of two allelochemicals from Ageratina adenophora (Spreng) on ABA, IAA and ZR contents in roots of upland rice seedlings. Allelopathy Journal, 21 (2): 253-262.

40- Wiese A.M., and Binning L.K., 1987. Calculating the threshold temperature of dormancy in seed of Osmorhiza claytonii L. American Journal of Botany, 78: 588-593.