Investigation of Forage Yield and Nutrients Uptake in Intercropping of Barley (Hordeum vulgare L.) and Grass Pea (Lathyrus sativus L.) Affected by Symbiosis with Glomus intraradices Fungus

بوم شناسی کشاورزی

Article 7, Volume 12, Issue 4 - Serial Number 46, December 2021, Pages 663-683   

Document Type: Scientific - Research

DOI: 10.22067/agry.2020.37589

Authors

Mohammad Haghaninia¹; Abdollah Javanmard^{* 2}; Sara Mollaaliabasiyan³

¹Agrotchnology- Crop ecology, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Iran

²Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Iran.

³Department of Soil Science and Engineering, Faculty of Agriculture, University of Maragheh, Iran.

Abstract

Introduction
Intercropping systems are one of the sustainable agricultural systems that defined as growing two or more plants simultaneously, lead to the use of more resources efficiently of nutrient water and land, improving plant productionIntercropping, as a new green revolution, is a sustainable strategy for the development of food production due to the lesser reliance of chemical fertilizer inputs compared with monocultures. In intercropping systems, plant nutrient uptake could improve the physical, chemical, and biological soil properties and higher nutrient mobilization in the rhizosphere. Arbuscular mycorrhizal fungi (AM) are preferred biofertilizers over other myriads of microorganisms that inhabit the interface between plant and soil. They are ubiquitous soil inhabitants and form the largest group which is predominantly associated with crops. Arbuscular mycorrhizal fungi can considerably improve plant growth, nutrients uptake, and transport, especially phosphorus, water status, and chlorophyll content. Previously studies demonstrated that higher productivity with AM fungi's application was attributed to higher nutrients availability such as P, K, Ca, Mg, etc. Thus, an experiment was conducted to evaluate the effects of barley's different intercropping patterns with grass pea and symbiosis with AM fungus on the forage yield and nutrients absorption, including N, P, K, Fe, Zn, Mn, Ca, and Mg.
 
Materials and Methods
In order to investigate the forage nutrients content and in intercropping of barley (Hordeum vulgare L.) with grass pea (Lathyrus sativus L.) under application of arbuscular mycorrhizal fungi, a field experiment was performed as a factorial layout based on randomized complete block design (RCBD) with ten treatments and three replications at the faculty of Agriculture, University of Maragheh, Iran, during 2017. The first factor included different planting patterns (monoculture of barley, monoculture of grass pea, 75% grass pea+ 25% barley, 50% grass pea+ 50% barley, 25% grass pea+ 75% barley), and the second factor was inoculated and non-inoculated with Glomus intraradices fungus. All data were statistically analyzed using analysis of variance (ANOVA) using MSTAT-C statistical software. The Duncan's multiple range test was used to compare means at a 5% probability level.
 
Results and Discussion
This study demonstrated that the macro and micronutrient content were significantly affected by different cropping patterns with the application of AM fungi. The greatest barley forage yield belonged to inoculated barley monoculture. Furthermore, the results demonstrated that the inoculated barley forage yield in monoculture was 47.48% more than the non-inoculated. The highest grass pea forage yield was achieved in monocultures and followed by a ratio of 75% grass pea+ 25% barley symbiosis with mycorrhizae fungus. Also, the highest nutrient content was achieved in grass pea monoculture with the application of AM fungi. Between different intercropping patterns, the highest nutrients content was obtained in the 75% grass pea+ 25% barley with the application of AM fungus. The higher nutrients uptake was attributed to increasing the absorption surface and improving nutrients availability with the application of AM fungi. Also, Varma et al. (2018) reported that the application of AM fungi in intercropping systems increased the transfer of the nutrients, especially nitrogen, to component plants that resulted in higher nutrients uptake in plants. These authors also reported that the higher nitrogen content with the application of AM fungi attributed to the higher activity of nitrate reductase and dikinase glucan enzymes that resulted in higher nitrogen availability for plants.
 
Conclusion
According to the results of this research, intercropping treatments of barley/grass pea with Glomus intraradices considerably influenced by the absorption of nutrients and forage yield. The content of concentration nitrogen, phosphorus, potassium iron, zinc, manganese, magnesium, and calcium were highest at all intercropping patterns coincided with the application of mycorrhiza fungi than the non-inoculated monoculture of barley. Also, between different intercropping patterns, the highest nutrients content was obtained in 75% grass pea+ 25% barley pattern accompanied by application of mycorrhiza that suggested as a better pattern to achievement high-quality forage for farmers. Therefore, intercropping of barley/grass pea with the application of mycorrhizae can improve forage quality of barley and grass pea from the point of view concentration of nutrients and were influential in production forage with high quantity.

Keywords

Bio-fertilizer; Nitrogen; Planting patterns; phosphorus; Sustainable agriculture

References

Agegnehu, G., Ghizaw, A., and Sinebo, W., 2006. Yield performance and land use efficiency of barley and faba bean mixed cropping in Ettiopian highlands. European Journal of Agronomy 25(3): 202-207.

Azimzadeh, Y., Shariatmadari, H., and Shirvani, M., 2015. Effects of green manure and mixed cultur of maize (Zea Mays L.) and canola (Brassica napus L.) on bioavailability and uptake of iron in greenhouse. Iranian Journal of Soil Research 29(2): 117-129. (In Persian with English Summary)

Boomsma, C.R., and Vyn, T.J., 2008. Maize drought tolerance: potential improvements through arbuscular mycorrhizal symbiosis. Field Crops Research 108: 14–31.

Bremner, J.M., and Mulvaney, C.S., 1982. Nitrogen-Total, (Eds.), Methods of Soil Analysis. Agronomy Monograph 9, Part 2, American Society of Agronomy 595-624.

Bukvićl, G., Antunović, M., Popović, S., and Rastija, M., 2003. Effect of P and Zn fertilisation on biomass yield and its uptake by maize lines (Zea mays L.). Plant, Soil Environment 49(11): 150-505.

Chen, Y., Shi, J., Tin, G., Zheng, S., and Lin, Q., 2004. Fe deficiency induces Cu uptake and accumulation in Commelia communis. Plant Science 166: 1371-1377.

Druege, U., Baltruschat, H., and Franke, P., 2007. Piriformospora indica promotes adventitious root formation in cuttings. Scientia Horticulturae 112: 422-426.

Ebrahimian, E., Bybordi, A., Seyyedi, S., and MohmmadiKia, R., 2015. Effects of the nitrogen and zinc fertilizers and salinity irrigation on yield, quality traits and nutrient uptake of canola (Brassica napus L. cv. Okapi). Journal of Agroecology7(1): 120-126. (In Persian with English Summary)

Eskandari, H., and Ghanbari, A., 2011. Evaluation of competition and complementarity of corn (Zea mays L.) and cowpea (Vigna sinensis L.) intercropping for nutrient consumption. Journal of AgrculturalScience and Sustainable Production21(2): 67-75. (In Persian with English Summary)

Estrada-Luna, A., and Davies, A., 2003. Arbuscular mycorrhizal fungi influence water relations, gas exchange, abscisic acid and growth of micropropagated chile ancho pepper (Capsicum annuum) plantlets during acclimatization and post-acclimatization. Journal of Plant Physiology 160: 1073-1083.

Ghasemi-Fasaei, R., and Mansoorpoor, Y., 2015. Metal micronutrients relationships in crop, soil, and common weeds of two maize (Zea mays L.) fields. Archives of Agronomy and Soil Science 61(12): 1733-1741.‏

Giles, C.D., Brown, L.K., Adu, M.O., Mezeli, M.M., Sandral, G.A., Simpson, R.J., Wendler, R., Shand, C.A., Menezes-Blackburn, D., Darch, T., Stutter, M.I., Lumsdon, D.G., Zhang, H., Blackwell, M.S.A., Wearing, C., Cooper, P., Haygarth, P.M., and George, T.S., 2017. Response-based selection of barley cultivars and legume species for complementarity: root morphology and exudation in relation to nutrient source. Plant Science 255: 12-28.

Guo, X., Xiong, X., Shen, H., Qiu, W., Ji, C., Zhang, Z.H., and Zuo, Y., 2014. Dynamics in the rhizosphere and iron-uptake gene expression in peanut induced by intercropping with maize: role in improving iron nutrition in peanut. Plant Physiology and Biochemistry 76: 36-43.

Hamzei, J., and Sadeghi Meabadi, F., 2013. Effects of mycorrhizal symbiosis on physiological indices and yield of grain sorghum under different irrigation intervals. Journal of Crops Improvement 15(4): 151-163.

Hauggaard-Nielsen, H., Ambus, P., and Jensen, E.S., 2003. The comparison of nitrogen use and leaching in sole cropped versus intercropped pea and barley. Nutrient Cycling in Agroecosystems 65(3): 289-300.

Inal, A., Gunes, A., Zhang, F., and Cakmak, I., 2007. Peanut-maize intercropping induced changes in rhizosphere and nutrient concentrations in shoots. Plant Physiology and Biochemistry 45(5): 350-356.

Kizhaeral, S.S., Virgine Tenshia, J.S., Jayalakshmi, K., and Ramachandran, V., 2011. Antioxidant enzyme activities in arbuscular mycorrhizal (Glomus intraradices) fungus inoculated and non-inoculated maize plants under zinc deficiency. Indian Journal of Microbiology 51(1): 37-43.

Lehmann, A., Veresoglou, S.D., Leifheit, E.F., and Rillig, M.C., 2014. Arbuscular mycorrhizal influence on zinc nutrition in crop plants–A meta-analysis. Soil Biology and Biochemistry 69: 123-131.

Li, W., Li, L., Sun, j., Guo, T., Zhang, F., Bao, X., Peng, A., and Tang, C., 2005. Effects of intercropping and nitrogen application on nitrate present in the profile of an orthicanthrosol in Northwest China. Agriculture, Ecosystems and Environment 105: 483-491.

Lindsay, W.L., and Norvell, W.A., 1978. Development of a DTPA soil test for Zn, Fe, Mn and Cu. Soil Science. Society of American Journal 42: 421-428.

Mahanta, D., Rai, R.K., Mishra, S.D., Raja, A., Purakayastha, T.J., and Varghese, E., 2014. Influence of phosphorus and biofertilizers on soybean and wheat root growth and properties. Field Crops Research 166: 1-9.

Marschner, H., 1995. Mineral Nutrition of Higher Plants. Second Ed. Academic Press, USA.

Meena, V.S., Maurya, B.R., and Verma, J.P., 2014. Does a rhizospheric microorganism enhance K⁺ availability in agricultural soils? Microbiological Research 169: 337-347.

Mench, M., and Martin, E., 1991. Mobilization of cadmium and other metals from two soils by root exudates of Zea mays L., Nicotiana tabacum L. and Nicotiana rustica L. Plant Soil 132(2): 187-196.

Nabati-nasaz, M., gholipouri, A., and Mostafavi Rad, M., 2015. Evaluation of forage yield and important agronomic indices of corn affected by intercropping systems with peanut and nitrogen rates. Journal of Agroecology 8(1): 70-81. (In Persian with English Summary)

Najafi, N., and Mostafae, M., 2015. Improvement of corn plant nutrition by farmyard manure application and intercropping with bean and bitter vetch in a calcareous soil. Journal of Soil Management and Sustainable Production 5(1): 1-22. (In Persian with English Summary)

Nelson, D.W., and Sommers, L.E., 1982. Total carbon, organic carbon and organic matter. p. 539-579. In A.L. Page (Ed.). Method of Soil Analysis, Chemical and Microbiological Methods, Part 2. ASA and SSSA, Madison, WI.

Nyoki, D., and Ndakidemi, P.A., 2018. Rhizobiuminoculation reduces P and K fertilization requirement in cornsoybean intercropping. Journal Rhizosphere 5: 51-56.

Olsen, S.R., and Sommers, L.E., 1982. In: A.L. Phosphorus, R.H. Miller and D.R. Keeney (Eds.). Methods of Soil Analysis. Part II: Chemical and Microbiological Properties. Madison, Wisconsin: American Society of Agronomy 403-427.

Pakgohar, N., and Ghanbari, A., 2013. Evaluation of competition and nutrient consumption of nutrifid millet and green pea in intercropping. Journal of Crops Improvement 15(4): 137-150. (In Persian with English Summary)

Phillips, J.M., and Hayman, D.S., 1970. Improved procedures clearing roots and staining parasitic and vesicular mycorrhizal fungi for rapid assessment of infection. Transaction of the British Mycological Society Journal 55: 158-161.

Rezvani moghaddam, P., Ehyaee, H.R., and Amiri, M.B., 2017. Application of spent mushroom compost and mycorrhiza on yield and yield components of garlic (Allium sativum L.) in the low input cropping system. Journal of Agroecology 9(2): 490-504. (In Persian with English Summary)

Sharda waman, M.K., and Bernard Felinov, R., 2009. Studies on effects of arbuscular mycorrhizal (AM) fungi on mineral nutrition of (Carica papaya L.). Notulae Botanicae Horti Agrobotanici Cluj 37: 183-186.

Soleimanpoor, L., Naderi, R., and Najafi-Ghiri, M., 2017. Evaluation of metal micronutrients uptake in cereal-legume intercropping. Journal of Crops Improvement 18(4): 1017-1031. (In Persian with English Summary)

Subramanian, K.S., Tenshia, J., Jayalakshmi, V., and Ramachandran, V., 2011. Antioxidant enzyme activities in arbuscular mycorrhizal (Glomus intraradices) fungus inoculated and non-inoculated maize plants under zinc deficiency. Indian Journal of Microbiology 51(1): 37-43.

Smith, S.E., and Read, D., 2008. Mycorrhizas in agriculture, horticulture and forestry. Mycorrhizal Symbiosis (Third Edition). p. 611-636.

Stoltz, E., and Nadeau, E., 2014. Effects of intercropping on yield, weed incidence, forage quality and soil residual N in organically grown forage maize (Zea mays L.) and faba bean (Vicia faba L.). Field Crops Research 169: 21-29.

Tosti, G., and Guiducci, M., 2010. Durum wheat- faba bean temporary intercropping: effects on nitrogen supply and wheat quality. European Journal of Agronomy 33: 157-165.

Tong-jian, X., Qing-song, Y., Wei, R., Guo-hua, X.U., and Qi-rong, S.H. 2010. Effect of inoculation with arbuscular mycorrhizal fungus on nitrogen and phosphorus utilization in upland rice-mungbean intercropping system. Journal Published by Elsevier Ltd 9: 528 -545.

Valentine, A.J., Mortimer, P.E., Lintnaar, A., and Borgo, R., 2006. Drought responses of arbuscular mycorrhuza grapevines. Symbiosis 3: 127-133.

Varma, A., Prasad, R., and Tuteja, N., 2018. Mycorrhiza-Nutrient Uptake, Biocontrol, Ecorestoration. Springer.

Verzeaux, J., Hirel, B., Dubois, F., Lea, P.J., and Tetu, T., 2017. Agricultural practices to improve nitrogen use efficiency through the use of arbuscular mycorrhizae: basic and agronomic aspects. Plant Science 264: 48-56.

Walter, H.R., and Lanyon, L.E., 1982. Magnesium, Calcium, Strontium and Barium. Methods of Soil Analysis. Chemical and microbiological properties, USA. 262-267.

Wang, Y.L., Almvik, M., Clarke, N., Eich-Greatorex, S., Ogaard, A.F., Krogstad, T., Lambers, H., and Clarke, J.L., 2015. Contrasting responses of root morphology and rootexuded organic acids to low phosphorus availability in three important food crops with divergent root traits. AoB PLANTS 7: 1-11.

Weisany, W., Raei, Y., Zehtab-Salmasi, S., and Sohrabi, Y., 2016. Effect of arbuscular mycorrhiza fungi on yield and yield components of common bean (Phaseolus vulgaris L.) and dill (Anethum graveolens L.) in mono and intercropping system. Journal of Agricultural Science and Sustainable Production 26(3): 1-19.

Westerm, R.L., 1990. Soil Testing and Plant Analysis. SSSA. Madison Wisconisn, USA.

Yousfi, S., Rabhi, M., Abdelly, C., and Gharsalli, M., 2009. Iron deficiency tolerance traits in wild (Hordeum maritimum L.) and cultivated barley (Hordeum vulgare L.). Comptes Rendus Biologies 332(6): 523-533. (In Persian with English Summary)

Zhang, F.S., and Li, L., 2003. Using competitive and facilitative interaction in intercropping systems enhances crop productivity and nutrient- use efficiency. Plant and Soil 248: 305-312.