Comparison of Effect of Sodium Silicate Particle Size in Nutritional Solution on Physiological Growth Trials of Maize Seedlings under Cadmium Stress

پژوهشهای زراعی ایران

Article 8, Volume 15, Issue 2 - Serial Number 46, July 2017, Pages 425-437   

Document Type: Research Article

DOI: 10.22067/gsc.v15i2.52432

Authors

B Saadatian^* ; M Kafi

Ferdowsi University of Mashhad

Abstract

Introduction Although silicon (Si) is the second most abundant element in the earth’s crust and its content in plants often reaches values of macronutrients, it is not listed among plant essential elements. However, the beneficial effects of Si in alleviation of various kinds of biotic stresses are well known. Concerning biotic stress, Si enhances, for instance, the resistance of plants to the pathogenic fungi, and it protects plants against and various kinds of insects. Silicon can also mitigate abiotic stresses in plants. Silicon can also reduce the negative effects of some toxic metals in plant species. Cadmium (Cd) is one of the most dangerous toxic metals for living organisms. It is a hazardous contaminant of food and through the food chains enters the human body as a cumulative poison. Contamination of agricultural soils by Cd represents a serious. Environmental problem in many countries and ranks high in food safety issues. Silicon was recently described as an effective substance for alleviation of Cd toxicity in some plants. The use of nano-compound material has given a lot of attention by the agricultural researchers, especially by those investigating seed characteristics, although their exact mechanisms of actions are not well understood. Nanomaterials, because of their tiny size, show unique characteristics. For example, they can change physico-chemical properties compared to bulk materials. They have greater surface area than bulk materials, and due to this larger surface area, their solubility and surface reactivity tend to be higher.

Materials and Methods This experiment was conducted in an environmentally controlled Research greenhouse in Department of Agronomy, Ferdowsi University of Mashhad, Iran. Corn (SC 704) seeds were germinated in a soil less growing system in cocopite. When the seedlings were at the two leaves stage of growth, they were transplanted hydroponic culture. Experiment was carried out as a factorial based using completely randomized design with four replications the. Treatments were included cadmium (CdSO4) stress levels (Zero, 50 and 100 μmol l-1) and sodium silicate treatments (control (Zero), Nano and micro particles with 2 mM concentration).

Results and Discussion The results showed that the effects of sodium silicate, cadmium and their interactions on membrane stability index, specific leaf area, stomatal conductance, Fv/Fm, height, stem diameter, leaf area, shoot dry weight, root dry weight and shoot to root weight ratio was significant. But interaction between cadmium and sodium silicate on relative water content and leaf chlorophyll index was not significant. By increasing of Cd concentration, membrane stability index, chlorophyll index, Fv/Fm, relative water content, specific leaf area, stomatal conductance, height, stem diameter, leaf area, shoot dry weight and root dry weight trials reduced compared to control significantly. In no cadmium condition, sodium silicate nano-particles application reduced height, stem diameter, leaf area and shoot dry weight 12.8, 9, 34.2 and 23.2% compared to control, respectively. In contrast, using of micro particles in non-stress condition, had a positive effect on above mentioned traits. But in 50 μmol l-1 Cd, nano-particles increased membrane stability index, specific leaf area, stomatal conductance, stem diameter and shoot dry weight trials, significantly. At highest concentration of cadmium, effect of micro particles on membrane stability index, stomatal conductance and shoot to root was higher than nano-particles. Also, using of nano particles had a positive effect on above mentioned traits in Cd stress condition. In general, application of nano particles in non-cadmium stress conditions had phytotoxicity effects on corn and only in cadmium stress condition, the effect of these particles showed their positive effect.

Conclusions In general, silicon nanoparticles were only beneficial effect of cadmium stress. Hence, the use of sodium silicate nanoparticles of was possible only in conditions of cadmium stress.

Keywords

Chlorophyll fluorescence; Correlation; Hydroponic culture; Nano-particles

References

1. Astolfi, S., Zuchi, S., and Passera, C. 2005. Effect of cadmium on H+ATPase activity of plasma membrane vesicles isolated from roots of different S-supplied maize (Zea mays L.) plants. Plant Science 169: 361-368.

2. Adtina, M. H., and Beasford, R. T. 1986. The effects of silicon on cucumber plants grown in recirculating nutrient solution. Journal Annual of Botany 58: 343-351.

3. Al-Aghabary, K., Zhu, Z., and Shi, Q. H. 2004. Influence of silicon supply on chlorophyll content, chlorophyll fluorescence, and antioxidative enzyme activities in tomato plants under salt stress. Journal of Plant Nutrient 27: 2101-2115.

4. Amooaghaie, R., Marefat, E., and Shabani, L. 2012. Interaction of salicylic acid and cadmium on growth, photosynthetic pigments and ion distribution in Arial parts of soybean plantlets. Journal of Plant Biology 14: 75-88. (in Persian with English abstract).

5. Balestrasse, K. B., Gardey, L., Gallego, S. M., and Tomaro, M. L. 2001. Response of antioxidant defence system in soybean nodules and roots subjected to cadmium stress. Australian Journal of Plant Physiology 28: 497-504.

6. Dabin, P., Marafanet, E., Miusny, J. M., and Myttenuere, C. 1978. Adsorption, Distribution and Binding of Cadmium and Zinc in irrigated rice plant. Plant and Soil 50: 32-36.

7. Epstein, E. 1999. Silicon, Annual Review. Plant Physiology and Plant Molecular Biology 50: 641-664.

8. Haghighi, M., and Pessarakli, M. 2013. Influence of silicon and nano-silicon on salinity tolerance of cherry tomatoes (Solanum lycopersicum L.) at early growth stage. Scientia Horticulture 161: 111-117.

9. Hoagland, D. R., and Arnon, D. I. 1950. The water-culture method for growing plants without soil. California Agricultural Experiment Station Circular 347: 1-32.

10. Joner, E. J., and Leyval, C. 2001. Time course of heavy metal uptake in maize and clover as affected by root density and different mycorrhizal inoculation regimes. Biology and Fertilizer and Soils 33: 351-357.

11. Liang, Y. C., Shen, Q. R. Shen, Z. G., and Ma, T. S. 1996. Effects of silicon on salinity tolerance of two barley cultivars. Journal of Plant Nutrition 19: 173-183.

12. Liang, Y. C., Wong, J. W. C., and Wei, L. 2005. Silicon-mediated enhancement of cadmium tolerance in maize (Zea mays L.) grown in cadmium contaminated soil. Chemosphere 58 (4): 475-483.

13. Liu, C. P., Li, F. B., Lou, C. L., Liu, X. M., Wang, S. H., Liu, T. X., and Li, X. D. 2009. Foliar application of two silica sols reduced cadmium accumulation in rice grains. Journal of Hazardous Materials 161(2-3): 1466-1472.

14. Lu, C. M., Zhang, C. Y, Wen, J. Q. Wu, G. R., and Tao, M. X. 2002. Research of the effect of nanometer materials on germination and growth enhancement of Glycine max and its mechanism. Soybean Science 21: 168-172 (in Chinese with English abstract).

15. Malčovska, M. S., Dučaiova, Z., Maslaňakova, I., and Bačkor, M. 2014. Effect of Silicon on Growth, Photosynthesis, Oxidative Status and Phenolic Compounds of Maize (Zea mays L.) Grown in Cadmium Excess. Water Air Soil Pollut. 225: 2056.

16. Maxwell, K., and Johnson, G. N. 2000. Chlorophyll fluorescence- a practical guide. Experimental of Botany 51: 659-668.

17. Mohsenzadeh, S., Malboobi, M. A., Razavi, K., and Farrahi-Aschtiani, S. 2006. Physiological and molecular responses of Aeluropuslagopoides (Poaceae) to water deficit. Environmental and Experimental Botany 56: 314-322.

18. Rasouli sedghiani, M., Ghara malki, T., Besharati, H., and Karimi, A. 2013. The effect of beneficial soil microorganisms on growth and Cd uptake by maize. Iranian journal of soil research 27 (2): 205-215. (in Persian with English abstract).

19. Ruffini, C. M., and Cremonini, R. 2009. Nanoparticles and higher plants. Caryologia 62 (2): 161-165.

20. Saadatian, B., and Kafi, M. 2015. Study of nutritional role of silicon nano-particles on physiological characteristics of minituber potato production. Journal of Plant Production Research 22: 173-189. (in Persian with English abstract).

21. Sairam, R. K. 1994. Effect of moisture stress on physiological activities of two con-trasting wheat genotypes. Indian Journal. Experimental of Biology 32: 594-597.

22. Sanita ta di toppi, L., and Gabbriella, R. 1999. Response to Cd in higher plant- Review. Environmental and Experimental Botany 45: 105-130.

23. Shekari; L., Kamelmanesh, M. M., and Sadeghi, F .2014. Effects of Irrigation Water Cadmium Concentration on Yield and Growth Traits of Green Pepper (Capsicum frutescens). Iranian journal of soil research 28: 636-646. (in Persian with English abstract).

24. Vaculik, M., Luxa, A., Luxova, M., Tanimoto, E., and Lichtscheid, I. 2009. Silicon mitigates cadmium inhibitory effects in young maize plants. Environmental and Experimental Botany 67: 52-58.

25. Vasssilve, A., Liadon, C. F., Matos, M. D. C., Ramalho, J. C., and Yordanov, I. 2002. Photosynthetic performance and content of some nutrients in cadmium -and copper- treated barley plants. Journal of Plant Nutrition 25: 2343-2360.

26. Wang, M., Zou, J., Duan, X., Jiang, W., and Liu, D. 2007. Cadmium accumulation and its effects on metal uptake in maize (Zea mays L.). Bioresource Technology 98: 82-88.

27. Wang, L., Zhou, Q., Ding, L., and Sun, Y. 2008. Effect of cadmium toxicity on nitrogen metabolism in leaves of Solanum Nigarum L. Journal of Hazard Material 154: 818-425.

28. Yanar, Y., Yanar, D., and Gebologlu, N. 2011. Control of powdery mildew (Leveillula taurica) on tomato by foliar sprays of liquid potassium silicate (K2SiO3). African Journal of Biotechnology 10: 3121-3123.

29. Zhang, G., Fukami, M., and Sekimoto, H. 2002. Influence of cadmium on mineral concentration and yield components in wheat genotypes differing in Cd tolerance at seedling stage. Field Crops Research 77: 93-98.

30. Zhu, Z. J., Wei, G. Q., Li, J., Qian, Q. Q., and Yu, J. Q. 2004. Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Science 167: 527-533.