Chlorophyll Fluorescence as an Index for Freezing Injury Evaluation in Sugar beet (Beta vulgaris)

پژوهشهای زراعی ایران

Article 7, Volume 15, Issue 3 - Serial Number 47, October 2017, Pages 615-626   

Document Type: Research Article

DOI: 10.22067/gsc.v15i3.51305

Authors

A Nezami¹; H. R Khazaei¹; M Dashti^{* 2}; E Eyshi Rezaei³; A. H Saeednejad⁴

¹Ferdowsi University of Mashhad

²Khorasan-e-Razavi Agricultural and Natural Resources Research and Education Center

³University of Bonn

⁴Payame Noor University (PNU),Tehran

Abstract

Introduction
A sustainable cropping system can be designed by choosing crops that can use the solar radiation and water more efficiently. Sugar beet (Beta vulgaris L.) is a highly productive crop which is widely under cultivation in different regions in Iran. Sugar beet production in Iran, scarcely limited by the solar radiation, but heavily relies on limited available water in such arid and semi-arid regions which makes the spring cultivation of it very expensive. Autumn cultivation of sugar beet is considered as an alternative to avoid water stress in Khorasan province, which is the main producing land of this crop in Iran. Variation of chlorophyll florescence parameter is an important criterion as well as suitable and nondestructive method which has been employed to determine the differences between plant species on resistance to environmental stresses such as freezing stress, and has been widely used as an important index to quantify the response of cold resistant varieties of corn and rice and heat resistance of sunflower cultivars.
Materials and Methods
In order to study the possibility of using the chlorophyll fluorescence parameters for evaluation freezing tolerance of sugar beet varieties, an experiment was performed by using a factorial based on randomized complete block design with three replications at agricultural faculty of Ferdowsi University of Mashhad. Seven sugar beet varieties (Jolge, Giada,Monatunno,SBSI1, Palma, Suprema,andPP8), exposed to ten freezing temperature levels (Zero, -2, -4, -6, -8, -10, -12, -14, -16 and -18), and their yield of quantum efficiency (ΔF/Fms) were measured in four levels of recovery periods (2, 12, 24 and 72 hours). Chlorophyll fluorescence was measured on control plants along with plants which were under 2, 12, 24, 72 hr. recovery after freezing. Measurements were carried out by using a portable fluorometer in the marginal region of the youngest fully expanded leaves.
Results and Discussion
Photochemical efficiency of photosystem II had strong regression with plant survival percentage (R2=0.90**). Monatunno variety had the highest and SBSI1 had the lowest photochemical efficiency of photosystem II. There were no differences between sugar beet varieties on photochemical efficiency of photosystem II until -14oC, but lower temperatures severely declined that parameter. There was a reduction on efficiency of photosystem II in the first 24 hr. duration of recovery, but after 72 hr. increased up to the before freezing conditions. Monatunno cultivar had the suitable recovery where exposed to the -16oC, but the efficiency of photosystem II on SBSI1 cultivar was decreased dramatically at the same temperature. Fluctuation trends of ΔF/Fmsbetween freezing treatments and recovery periods showed sensible decrease trend until -14oC, but negative trend started at -16oC and reached to lowest values at -18oC in recovery period. Hasselt (1996) showed significant decrease in (ΔF/Fms) between wheat varieties started at temperatures lower than -8oC. Fluctuation trend of ΔF/Fms parameter of Monatunna and Sbsi1 varieties as resistant and sensitive varieties with respectively highest and lowest survival percentage under severe freezing treatments (-14, -16 and -18oC) indicated clear difference between Monatunna and Sbsi1 at -16oC.
Conclusions
Yield of quantum efficiency of sugar beet varieties showed highest differences in recovery period, this parameter quantity in Monatunna as a resistant genotype recovered near to before stress conditions during recovery period (except -18oC freezing treatment) but Sbsi1 as a sensitive variety did not show recovery especially in severe freezing levels. Based on this study, we can determine Monatunna variety as high resistant to freezing stress with quick recovery period compared to other study varieties in early growth period which is almost the most important part of growth cycle of sugar beet varieties.

Keywords

Efficiency of photosystem II; Recovery period; Sugar beet; Survival

References

1. Baker, N. R., and Rosenquist, E. 2004. Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. Journal of Experimental Botany 55: 1607-1621.

2. Clement, J. M. A. M., and Van Hasselt, P. R. 1996. Chlorophyll fluorescence as a parameter for frost hardiness in winter wheat: a comparison with other hardiness parameters. Phyton (Horn, Austria) 36 (1): 29-56.

3. Dai, F., Zhou, M., and Zhang, G. 2007. The change of chlorophyll fluorescence parameters in winter barley during recovery after freezing shock and as affected by cold acclimation and irradiance. Plant Physiology and Biochemistry 45: 915-921.

4. Dashti, M., Kafi, M., Tavakkoli, H., Mirza, M., and Nezami, A. 2015. Effects of freezing stress on Morpho-physiological indices and chlorophyll fluorescence of Salvia leriifolia Benth. Seedlings. Iranian Journal of Plant Research 28 (5): 962-973.

5. Dobrowski, S. Z., Pushnik, P. J., Tejada, J. C., and Ustin, S. L. 2005. Simple reflectance indices track heat and water stress-induced changes in steady-state chlorophyll fluorescence at the canopy scale. Remote Sensing Environment 97: 403-414.

6. Gray, G. R., Chauvin, L. P., Sarhan, F., and Huner, N. P. A. 1997. Cold acclimation and freezing tolerance: a complex interaction of light and temperature. Plant Physiology 114: 467-474.

7. Jalilian, A., Mazaheri, D., Tavakkol Afshari, R., Abdollahian-Noghabi, H., Rahimian, H., and Ahmadi, A. 2002. Effect of freezing damage at seedling stage in different sugar beet cultivars. Iranian Journal of Crop Science 10: 400-415. (in Persian with English abstract).

8. Majdi, M., Karimzadeh, G., and Mahfoozi, S. 2007. Effects of low temperature and exogenous calcium on the quantum efficiency of photosystem II (Fv/Fm) and relative content of chlorophyll in cold susceptible and tolerant wheat cultivars. Pajouhesh Sazandegi 77: 175-181. (in Persian with English abstract).

9. Nasiri Mahallati, M., Koocheki, A., Rezvani Moghadam, P., and Beheshti, A. 2007. Agroecology. Ferdowsi University of Mashhad press. (in Persian with English abstract).

10. Neuner, G., and Larcher, W. 1990. Determination of differences in chilling susceptibility of two soybean varieties by means of in vivo chlorophyll fluorescence measurement. Crop Science 167: 73-80.

11. Nezami, A., Borzooei, A., Jahani, M., Azizi, M., and Sharif, A. 2007. Electrolyte leakage as an indicator of freezing injury in colza (Brassica napus L.). Iranian Field Crop Research 1: 167-175. (in Persian with English abstract).

12. Oliveira, J. G., Alves, P. C. A., and Vitoria, A. P. 2009. Alterations in chlorophyll a fluorescence, pigment concentrations and lipid peroxidation to chilling temperature in coffee seedlings. Environmental and Experimental Botany 67: 71-76.

13. Petite, A. M., Rueda, A. M., and Lacuesta, M. 2005. Effect of cold storage treatments and transplanting stress on gas exchange, chlorophyll fluorescence and survival under water limiting conditions of Pinus radiata stock-types. European Journal of Forest Research 124: 73-82.

14. Rinaldi, M., and Vonella, A. V. 2006. The response of autumn and spring sown sugar beet (Beta vulgaris) to irrigation in Southern Italy: Water and radiation use efficiency. Field Crop Research 95: 103-114.

15. Scott, R. K., English, S. D., Wood, D. W., and Unsworth, M. H. 1973. The yield of sugar beet in relation to weather and length of growing season. The Journal of Agricultural Science 81: 339-347.

16. Strauss, A. J., Kruger, G. H. J., Strasser, R. J., and Heerden, P. D. R. 2006. Ranking of dark chilling tolerance in soybean genotypes probed by the chlorophyll a fluorescence transient O-J-I-P. Environment and Experimental Botany 56: 147-157.