Evaluation of probiotic and antifungal properties of predominant LAB isolated from quinoa sourdough

مجله پژوهشهای علوم و صنایع غذایی ایران

Article 17, Volume 17, Issue 4 - Serial Number 70, September and October 2021, Pages 647-657   

Document Type: Research Article

DOI: 10.22067/ifstrj.v18i1.83240

Authors

Elham Rouhi¹; Alireza Sadeghi^{* 1}; Seyed Mahdi Jafari¹; Mohammad Abdolhoseini²; Elham Assadpour³

¹Department of Food Science & Technology, Gorgan University of Agricultural Sciences & Natural Resources.

²Department of Water Engineering, Gorgan University of Agricultural Sciences & Natural Resources.

³Department of Food Science & Technology, Baharan University, Gorgan, Iran.

Abstract

< p >Introduction: Evaluation of probiotic and antifungal properties of lactic acid bacteria (LAB) isolated from fermented substrates has great importance in order to provide microbial cultures for fermentation industries. Among the fermented foods, dairy products play the main role as carriers of probiotics. Meanwhile, non-dairy fermented foods have been rarely studied in order to isolation and characterization of their probiotic microorganisms. Sourdough as a mixture of flour and water is a proper fermented ecosystem to isolate probiotic and antifungal LAB. Besides their desired health, probiotics must become active in the consumer''s gastrointestinal tract without any adverse effect. These bacteria can be used as starter, adjunct or preservative cultures to produce different fermented foods. Furthermore, antimicrobial metabolites of the LAB have also numerous potential applications as bio-preservatives in food and/or medical technologies. 
< p > 
< p >Materials and methods: In the present study, after continuous back-slopping process, predominant LAB was isolated from fermented quinoa. Then the LAB isolate was identified using PCR amplification of its partial 16S rDNA gene. Subsequently, probiotic properties of the LAB including its resistance to low pH and bile salt, antibacterial effects, aggregation potentials, antibiotic susceptibility and haemolytic activity were investigated. Antifungal effect of the LAB on Aspergillus niger was also determined using overlay bioassay. Finally, the one way analysis of variance (ANOVA) with the least significant difference (LSD) post hock (at p < /em> 
< p >Results and discussion: Sequencing results of the PCR products led to the identification of Enterococcus hirae as predominant LAB isolated from quinoa sourdough. Sourdough fermentation depends on the several technological and environmental factors and therefore, different types of these complex stressful ecosystems have their specific microflora with unique properties. The LAB isolate had proper survival after continuous pH and bile treatments. Resistance to low pH and bile salt is not sufficient to predict the survival of the probiotics in the actual conditions of the gastrointestinal tract. However, these properties are necessary for assessment of viability and activity in this situation. Furthermore, the highest antibacterial activity of the LAB was observed against Bacillus cereus among the studied food borne indicator bacteria. The effect of crude cell free supernatant (CFS) obtained from LAB isolate on indicator bacteria was significantly (p < /em>

Keywords

Quinoa Sourdough; Predominant LAB Isolate; Back-Slopping Process; Probiotic; Protective Culture

References

Abnous, K., Brooks, S.P., Kwan, J., Matias, F., Green-Johnson, J., Selinger, L.B., Thomas, M., & Kalmokoff, M. 2009. Diets enriched in oat bran or wheat bran temporally and differentially alter the composition of the fecal community of rats. The Journal of Nutrition, 139(11), 2024-2031.

Abriouel, H., Muñoz, M.D.C.C., Lerma, L.L., Montoro, B.P., Bockelmann, W., Pichner, R., Kabisch, J., Cho, G.S., Charles Franz, M.A.P.Gálvez, A., & Benomar N. 2015. New insights in antibiotic resistance of Lactobacillus species from fermented foods. Food Research International, 78, 465-481.

Abushelaibi, A., Al-Mahadin, S., El-Tarabily, K., Shah, N.P., & Ayyash, M. 2017. Characterization of potential probiotic lactic acid bacteria isolated from camel milk. LWT-Food Science and Technology, 79, 316-325.

Adisa, A.M., & Ifesan, B.O.T. 2016. Probiotic potential of lactic acid bacteria (lab) isolate from wholegrain millet sourdoughs. Annals. Food Science and Technology, 17(2), 458-468.

Angmo, K., Kumari, A., & Bhalla, T.C. 2016. Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT-Food Science and Technology, 66, 428-435.

AOAC. 2005. Official methods of analysis (18 ed). Gaithersburg, MD: AOAC International.

Axel, C., Brosnan, B., Zannini, E., Peyer, L.C., Furey, A., Coffey, A., & Arendt, E.K. 2016. Antifungal activities of three different Lactobacillus species and their production of antifungal carboxylic acids in wheat sourdough. Applied Microbiology and Biotechnology, 100(4), 1701-1711.

Axel, C., Röcker, B., Brosnan, B., Zannini, E., Furey, A., Coffey, A., & Arendt, E.K. 2015. Application of Lactobacillus amylovorus DSM19280 in gluten-free sourdough bread to improve the microbial shelf life. Food Microbiology, 47, 36-44.

Axelsson, L.T., Chung, T.C., Dobrogosz, W.J., & Lindgren, S.E. 1989. Production of a broad spectrum antimicrobial substance by Lactobacillus reuteri. Microbial Ecology in Health and Disease, 2, 131–136.

Bernardeau, M, Vernoux, J.P, Henri Dubernet, S., & Guegen, M. 2008. Safety assessment of dairy microorganisms: The Lactobacillus genus. International Journal of Food Microbiology, 126, 278–285.

Campana, R., van Hemert, S., & Baffone, W. 2017. Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion. Gut Pathogens, 9(1), 12.

Casarotti, S.N., Carneiro, B.M., & Penna, A.L.B. 2014. Evaluation of the effect of supplementing fermented milk with quinoa flour on probiotic activity. Journal of Dairy Science, 97(10), 6027-6035.

Collado, M.C., Meriluoto, J., & Salminen, S. 2007. In vitro analysis of probiotic strain combinations to inhibit pathogen adhesion to human intestinal mucus. Food Research International, 40(5), 629-636.‏

Delgado, S., O''sullivan, E., Fitzgerald, G., & Mayo, B. 2007. Subtractive screening for probiotic properties of Lactobacillus species from the human gastrointestinal tract in the search for new probiotics. Journal of Food Science, 72(8), M310-M315.

Demirbaş, F., İspirli, H., Kurnaz, A.A., Yilmaz, M.T., & Dertli, E. 2017. Antimicrobial and functional properties of lactic acid bacteria isolated from sourdoughs. LWT-Food Science and Technology, 79, 361-366.

García-Cayuela, T., Korany, A.M., Bustos, I., de Cadiñanos, L.P.G., Requena, T., Peláez, C., & Martínez-Cuesta, M.C. 2014. Adhesion abilities of dairy Lactobacillus plantarum strains showing an aggregation phenotype. Food Research International, 57, 44-50.

Han, Q., Kong, B., Chen, Q., Sun, F., & Zhang, H. 2017. In vitro comparison of probiotic properties of lactic acid bacteria isolated from Harbin dry sausages and selected probiotics. Journal of Functional Foods, 32, 391-400.

Kunchala, R., Banerjee, R., Mazumdar, S.D., Durgalla, P., Srinivas, V., & Gopalakrishnan, S. 2016. Characterization of potential probiotic bacteria isolated from sorghum and pearl millet of the semi-arid tropics. African Journal of Biotechnology, 15(16), 613-621.

Magnusson, J., Ström, K., Roos, S., Sjögren, J., & Schnürer, J. 2003. Broad and complex antifungal activity among environmental isolates of lactic acid bacteria. FEMS Microbiology Letters, 219(1), 129-135.‏

Manini, F., Casiraghi, M.C., Poutanen, K., Brasca, M., Erba, D., & Plumed-Ferrer, C. 2016. Characterization of lactic acid bacteria isolated from wheat bran sourdough. LWT-Food Science and Technology, 66, 275-283.‏

Matejčeková, Z., Liptáková, D., & Valík, Ľ. 2017. Functional probiotic products based on fermented buckwheat with Lactobacillus rhamnosus. LWT-Food Science and Technology, 81, 35-41.‏

Ogunsakin, A.O., Vanajakshi, V., Anu-Appaiah, K.A., Vijayendra, S.V.N., Walde, S.G., Banwo, K., Sanni, A.I., & Prabhasankar, P. (2017). Evaluation of functionally important lactic acid bacteria and yeasts from Nigerian sorghum as starter cultures for gluten-free sourdough preparation. LWT-Food Science and Technology, 82, 326-334.‏

Oliveira, P.M., Brosnan, B., Furey, A., Coffey, A., Zannini, E., & Arendt, E. K. 2015. Lactic acid bacteria bioprotection applied to the malting process. Part I: strain characterization and identification of antifungal compounds. Food Control, 51, 433-443.

Rajoka, M.S.R., Mehwish, H.M., Siddiq, M., Haobin, Z., Zhu, J., Yan, L., Shao, D., Xu, X., & Shi, J. 2017. Identification, characterization, and probiotic potential of Lactobacillus rhamnosus isolated from human milk. LWT-Food Science and Technology, 84, 271-280.

Reller, L.B., Weinstein, M., Jorgensen, J.H., & Ferraro, M.J. 2009. Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clinical Infectious Diseases, 49(11), 1749-1755.

Rizzello, C.G., Lorusso, A., Montemurro, M., & Gobbetti, M. 2016. Use of sourdough made with quinoa (Chenopodium quinoa) flour and autochthonous selected lactic acid bacteria for enhancing the nutritional, textural and sensory features of white bread. Food Microbiology, 56, 1-13.

Rojo-Bezares, B., Sáenz, Y., Poeta, P., Zarazaga, M., Ruiz-Larrea, F., & Torres, C. 2006. Assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine. International Journal of Food Microbiology, 111(3), 234-240.

Ruiz Rodríguez, L., Vera Pingitore, E., Rollan, G., Cocconcelli, P. S., Fontana, C., Saavedra, L., G. Vignolo  &  Hebert, E. M. 2016. Biodiversity and technological, functional potential of lactic acid bacteria isolated from spontaneously fermented quinoa sourdoughs. Journal of Applied Microbiology, 120(5), 1289-1301.

Russo, P., Arena, M.P., Fiocco, D., Capozzi, V., Drider, D., & Spano, G. 2017. Lactobacillus plantarum with broad antifungal activity: A promising approach to increase safety and shelf-life of cereal-based products. International Journal of Food Microbiology, 247, 48–54.

Sadeghi, A., Ebrahimi, M., Raeisi, M., & Nematollahi, Z. 2019. Biological control of foodborne pathogens and aflatoxins by selected probiotic LAB isolated from rice bran sourdough. Biological Control, 130, 70-79.‏

Saxelin, M., Tynkkynen, S., Mattila-Sandholm, T., & de Vos, W.M. 2005. Probiotic and other functional microbes: from markets to mechanisms. Current Opinion in Biotechnology, 16(2), 204-211.

Sharma, P., Tomar, S.K., Sangwan, V., Goswami, P., & Singh, R. 2016. Antibiotic resistance of Lactobacillus sp. isolated from commercial probiotic preparations. Journal of Food Safety, 36(1), 38-51.

Shahrestani, F. F., Ebrahimi, M. T., Bayat, M., Hashemi, J., & Razavilar, V. 2019. Reduction of aflatoxin M1 by three acid-and bile-resistant antifungal probiotics vs. natamycin in milk. Biomedical Research, 30(1), 122-126.

Zhang, Y., Zhang, L., Du, M., Yi, H., Guo, C., Tuo, Y., Han, X., Li, J., Zhang, L., & Yang, L. 2011. Antimicrobial activity against Shigella sonnei and probiotic properties of wild lactobacilli from fermented food. Microbiological Research, 167(1), 27-31