The Effect of Withholding Irrigation and Foliar Application of Zn and Mn on Yield and Eco-physiological Characteristics of Rapeseed (Brassica napus L.)

پژوهشهای زراعی ایران

Article 7, Volume 18, Issue 1 - Serial Number 57, April 2020, Pages 85-100   

Document Type: Research Article

DOI: 10.22067/gsc.v18i1.80050

Authors

G Khodabin¹; Z Tahmasebi-Sarvestani^{* 1}; A. H Shirani Rad²; S. A. M Modarres-Sanavy¹; E Bakhshande³

¹Tarbiat Modares University

²Seed and Plant Improvement Institute

³Sari Agricultural Sciences and Natural Resources University

Abstract

Introduction
Development of new canola (Brassica napus L.) varieties need effective tools to monitor characterizes association in yield and its components. Although, determination of the response of oil seeds cultivars to environmental variables is one of the principal of agriculture planning to achieve maximum qualitative and quantitative yield. Water deficit stress and sufficient nutrition are the most important factors limiting yield production by changing the physiological processes of the plant. Iran is considered as the arid and semi-arid with average rainfall of 250 mm. On the other hand, 33% of agriculture land is devoted to dry cultivation. Water deficit by affecting on vegetative and reproductive growth period and balance between them will change yield and product quality.
Materials and Methods
In order to study the responses of three winter canola cultivars to late season drought stress and foliar application of Mn and Zinc sulfate fertilizer on physiological, morphology characteristics and yield, a split factorial experiment was carried out based on randomized complete block design with three replications in 2017-2018 at the Karaj province. Irrigation in two levels, normal and water holding at flowering stage to next, foliar application in four levels, sprayed with water (control), foliar application with zinc sulfate, foliar application with Mn sulfate, foliar application composition with zinc sulfate and Mn sulfate (each of them was with concentration of four per thousand) both in main plot and three cultivars included Nima (control) and two new lines KS7 and R15 in sob plot. Foliar application was applied during the stem elongation stage. When the 50% of pod appeared total chlorophyll and leaves carbohydrate solution content was measured as index of drought stress damage. Eight traits were measured on 10 random plants per plot. The traits were plant height, number of pods /plant, number of seeds/pod, 1000 seed weight, seed yield, biomass yield, oil percentage and oil yield.
Results and Discussion
The results showed all characteristics significantly were influenced by water holding and foliar application. Interaction of water holding and foliar application was significant in attributes of plant height and oil content. Main effect of cultivar and interaction of water holding and cultivar were significant in all treatment except oil content. Due to water holding increasing in soluble carbohydrates and reducing the concentration of chlorophyll was occurred. The yield components of canola because of irrigation disruption decreased which leads to lower grain and rapeseed oil yield. The results showed that in normal irrigation, the highest seed yield (5168 kg.ha-1) oil yield (2244 kg.ha-1) and water use efficiency (0.99 %) were related to R15 line but in water holding condition the Nima cultivar obtained the highest achievements. In complete irrigation, foliar application of the two micro elements increased the traits studied. Most of the seed oil yield (1728 kg.ha-1), the weight of one thousand seeds (3.21 g), biological yield (15675 kg.ha-1) and water use efficiency were related to the main effect of zinc sulfate and Mn sulfate combination. Spraying plants with zinc sulfate and Mn sulfate improved plant height and increased oil content in drought stress condition in relation to spray by water (control). Among cultivars, Nima cv. had better outperformed relative to KS7 and R15 in normal irrigation. In water holding treatment, two cultivars R15 and Nima narrowly to each other had better outperformed relative to KS7. Non significant interaction effects of foliar application and cultivars for seed and oil yield indicated that all cultivars had similar trend. Drought not only causes dramatic loss of pigments but also leads to disorganization of thylakoid membrane, therefore reduction in chlorophyll contents is expected. The water holding condition in late season may influence plant growth that can be attributed to the lose yield components.
Conclusions
Correlation between drought situation and yield components in all cultivars, identify the most suitable indicators for monitoring drought tolerance cultivars. According to these results, R15 can be recommended for semiarid regions due to maximum seed and oil yield among and non stress condition. In water deficit condition R15 can be recommended for semiarid regions due to maximum seed and oil yield among and non stress condition.

Keywords

Micro Nutrition; Water deficit; Water use efficiency; Yield and component yield

References

1. Aein, A. 2011. Changes in the amount of proline, carbohydrate solution and potassium, zinc and calcium absorption in sesame genotypes. (Sesamum indicum L.) under drought stress. Crop production under environmental stress conditions 4 (3): 39-48. (in Persion).

2. Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts, polyphenoxidase in beta vulgaris. Plant Physiology 24: 1-15.

3. Balaji, S., Kalaivani, T., and Rajasekaran, C. 2014. Biosorption of Zinc and Nickel and Its Effect on Growth of Different Spirulina Strains. Clean – Soil, Air, Water 42 (4): 507-512.

4. Bameri, M., Abdolshahi, R., Mohammadi-Nejad, Gh., Yousefi, Kh., and Tabatabaie, S. M. 2012. Effect of different microelement treatment on wheat (triticum aestivum) growth and yield. International Research Journal of Applied and Basic Sciences 3 (1): 219-223.

5. Borg, M., and Berger, F. 2015. Chromatin remodelling during male gametophyte development. The Plant Journal 83 (1): 177-188.

6. Bybordi, A., and Mamedov, G. 2010. Evaluation of Application Methods Efficiency of Zinc and Iron for Canola (Brassica napus L.). Notualea Science Biological 2 (1): 94-103.

7. Cakmak, I., McLaughlin, M. J., and White, P. 2017. Zinc for better crop production and human health. Plant and Soil 411: 1-4.

8. Candan, N., Cakmak, I., and Ozturk, L. 2018. Zinc‐biofortified seeds improved seedling growth under zinc deficiency and drought stress in durum wheat. Journal of Plant Nutrition and Soil Science 181 (3): 388-395.

9. Din, J., Khan, S. U., Ali, I., and Gurmani, A. R. 2011. Physiological and agronomic response of canola varieties to drought stress. The Journal of Animal and Plant Sciences 21 (1): 78-82.

10. Dubois, M., Gilles, K. A., Hamilton, J. K., Reber, P. A., and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Annual Chemistry 28: 350-356.

11. El-Din, H., El-Beltagi, S., and Mohamed, A. A. 2010. Variations in fatty acid composition, glucosinolate profile and some phytochemical contents in selected oil seed rape (Brassica napus L.) cultivars. Grasas y Aceites 61 (2): 141-150.

12. Faize, M., Nicola´s, E., Faize, L., Dı´az-Vivancos, P., Burgos, L., and Herna´ndez, J. A. 2015. Cytosolic ascorbate peroxidase and Cu, Zn-superoxide dismutase improve seed germination, plant growth, nutrient uptake and drought tolerance in tobacco. Theor. Experimental Plant Physiology 27: 215-226.

13. Geng, Y. J., Chen, L., Yang, C., Jiao, D. Y., Zhang, Y. H., and Cai, Z. Q. 2017. Dry-season deficit irrigation increases agricultural water use efficiency at the expense of yield and agronomic nutrient use efficiency of Sacha Inchi plants in a tropical humid monsoon area. Industrial Crops & Products 109: 570-578.

14. Hanif, M. A., Nawaz, H., Ayub, M. A., Tabassum, N., Kanwal, N., Rashid, N., Saleem, M., and Ahmad, M. 2017. Evaluation of the effects of zinc on the chemical composition and biological activity of basil essential oil by using Raman spectroscopy. Industrial Crops Product 96: 91-101.

15. Hassan-Zade, M., Naderi Darbaghshahi, M. R., and Shirani Rad, A. H. 2005. Evaluation of drought stress effects on yield and yield components of autumn rapeseed varieties in Isfahan region. Iranian Journal of Research in Agriculture 2 (2): 51- 62. (in Persian).

16. Imran and Ali Khana, A. 2017. Canola Yield and Quality Enhanced with Sulphur Fertilization. Russian Agricultural Sciences 43 (2): 113-119.

17. Jamshidi, N., Shirani rad, A. H., Takht chin, F., Nazeri, P., and Ghafari, M. 2012. Evaluation of Rapeseed Genotypes under Drought Stress Condition. Journal of Crop Ecophysiology 6 (3): 323-339. (in Persian).

18. Kanwal, N., Hanif, M. A., Khan, M. M., Ansari, T.M., and Khalil-Ur-Rehman, R. 2016. Effect of micronutrients on vegetative growth and essential oil contents of Ocimum sanctum. Journal Essential Oil Bearing Plants 19: 980-988.

19. Keerthi, P., Pannu R. K., and Dhaka A. K. 2017. Effect of sowing dates and nitrogen levels on total dry matter and its partitioning at different growth stages and yield of Indian mustard (Brassica juncea L.). Agricultural Science Digest 37 (1): 27-31.

20. Khajepour, M. R. 2001. Industrial plants. Publications Unit, University Jihad of Isfahan., 571 pages. (in Persion).

21. Khan, M. A., Ashraf, M. Y., Mujtaba, S. M., Shirazi, M. U., Khan, M. A., Shereen, A., Mumtaz, S., Aqil Siddiqui M., and Murtaza Kaleri, G. 2010. Evaluation of high yielding canola type Brassica genotypes/mutants for drought tolerance using physiological indices as screening tool. Pakistan Journal of Botany 42 (6): 3807-3816.

22. Lalinia, A. A., Khamenh, M., Galostian, M., Majnoon Hoseini, N., and Esmailzade Bahabadi, S. 2012. Echophysiological impact of water stress on growth and development of mungbean. International Journal of Agronomy and Plant Production 3: 599-607.

23. Ma, D., Sun, D., Wang, C., Ding, H., Qin, H., Hou, J., Huang, X., Xie, Y., and Guo, T. 2017. Physiological responses and yield of wheat plants in zinc-mediated alleviation of drought stress. Frontiers in Plant Science 8. DOI: 10.3389/fpls.2017.00860.

24. Marreiro, D. D. N., Cruz, K. J. C., Morais, J. B. S., Beserra, J. B., Severo, J. S., and Oliveira, A. R. S. 2017. Zinc and oxidative stress: current mechanisms. Antioxidants 6. DOI: 10.3390/ antiox6020024.

25. Molazem, D., Azimi, J., Ghasemi, M., Hanifi, M., and Khatami, A. 2013. Correlation analysis in different planting dates and plant density of canola (Brassica napus L.) varieties in Astara Region. Life Science Journal 10 (1): 26-31.

26. Obaid, E. A., and Al-Hadethi M. A. E. 2013. Effect of Foliar Application with Manganese and Zinc on Pomegranate Growth, Yield and Fruit Quality. Journal of Horticultural Science and Ornamental Plants 5 (1): 41-45.

27. Pirdashti, H., Tahmasebi – Sarvestani, Z., and Bahmanyar, M. A. 2009. Comparison of physiological response among four contrast rice cultivars under drought stress conditions. World Academy of Science, Engineering and Technology 49: 52-53.

28. Qaswar, M., Hussain, S., and Rengel, Z. 2017. Zinc fertilisation increases grain zinc and reduces grain lead and cadmium concentrations more in zinc-biofortified than standard wheat cultivar. Science Total Environment 605: 454-460.

29. Rajabi, M., Fetri, M., Ghobadi, M. E., Faraji, M. H., and Asadian, Gh. 2013. Foliar application of Zn and Mn fertilizers on yield and yield components of safflower (Carthamus tinctorius L.) cultivars. International Journal of Agricultural Crop Scince 5: 718-822.

30. Rameeh, V. 2014. Evaluation of planting dates effects on growth, phenology and seed yield of spring rapeseed varieties. Oil Plant Production 79-89. (in Persion).

31. Rezaeieh, K. A. P., Gurbuz, B., and Eivazi, A. 2016. Effects of different zinc levels on vegetative growth and essential oil contents of some Iranian and Turkish cumin (Cumin cyminum L.) genotypes. Journal of Essential Oil Bearing Plants 19: 1181-1191.

32. Sabaghnia, N., Dehghani, H., Alizadeh, B., and Mohghaddam, M. 2010. Interrelationships between seed yield and 20 related traits of 49 canola (Brassica napus L.) genotypes in non-stressed and water-stressed environments. Spanish Journal of Agricultural Research 8 (2): 356-370.

33. Sadeghinejad, A. A., Modarres Sanavi, S. A. M., Tabatabaei, S. A., and Modares Vameghi, S. M. 2014. Effect of water deficit stress at various growth stages on yield, yield components and water use efficiency of five rapeseed (Brassica napus L.) cultivars. Water and Soil Science 24 (2): 53-64. (in Persian).

34. Sepehri, A., and Golparvar, A. R. 2011. The eEffect of drought stress on water relations, chlorophyll content and leaf area in canola cultivars (Brassica napus L.). Electronic Journal of Biology 7 (3): 49-53.

35. Sevanto, S. 2018. Drought impacts on phloem transport. Current Opinion in Plant Biology 43: 76-81.

36. Sieling, K., Böttcher, U., and Kage, H. 2017. Sowing date and N application effects on tape root and above-ground dry matter of winter oilseed rape in autumn. Europe Journal of Agriculture 83: 40-46.

37. Slafer, G. A., and Whitechurch, E. M. 2001. Manipulation wheat development to improve adaptation. In: Reynolds M. P., Ortiz- Monasterio J. I., and McNab A. (eds). Application physiology in wheat breeding. Mexico, D. F, CIMMYT. pp: 160-170.

38. Soleimani, R. 2005. The integrated effects of foliar and soil application of Zn, Fe and Mn on yield and yield compounds of wheat in Ilam. Final Report of Research Project. Soil and Water Research Institute, Iran. 1222 (in Persian).

39. Sperotto, R. A., Ricachenevsky, F. K., de Waldow, V., Müller, A. L. H., Dressler, V. L., and Fett, J. P. 2013. Rice grain Fe, Mn and Zn accumulation: How important are flag leaves and seed number? Plant, Soil and Environment 59: 262-266.

40. Valli, S. P., Sudhakar, C., Rani, J., and Rajeswari, R. R. 2016. Correlation and path coefficient analysis for the yield components of safflower germplasm (Carthamus tinctorius L.). Electronic Journal Plant Breeding 7: 420-426.

41. Xu, C., McDowell, N. G., Sevanto, S., and Fisher, R. A. 2013. Our limited ability to predict vegetation dynamics under water stress. New Phytology 200: 298-300.

42. Xu, W., Cui, K., Xu, A., Nie, L., Huang, J., and Peng, Sh. 2015. Drought stress condition increases root to shoot ratio via alteration. Acta Physiol Plant. 37: 9. https://doi.org/10.1007/s11738-014-1760-0

43. Yang, M., Shi, L., Xu, F. S., Lu, J. W., and Wang, Y. H. 2009. Effects of B, Mo, Zn and their interactions on seed yield of rapeseed (Brassica napus L.). Pedosphere 19 (1): 53-59.

44. Zahoor, R., Dong, H., Abid, M., Zhao, W., Wang, Y., and Zhou, Zh. 2017. Potassium fertilizer improves drought stress alleviation potential in cotton by enhancing photosynthesis and carbohydrate metabolism. Environmental and Experimental Botany 137: 73-83.