Impact of Different Tree Compositions and Soil Properties on Soil Microbial Community

آب و خاک

Article 6, Volume 31, Issue 5 - Serial Number 55, November 2018, Pages 1409-1422   

Document Type: Research Article

DOI: 10.22067/jsw.v31i5.62819

Authors

samaneh Tajik¹; shamsollah Ayoubi^{* 1}; jahangir khajehali²; shaban shataee³

¹Isfahan University of Technology

²Isfahan University of technology

³Gorgan university of agricultural sciences and natural resources

Abstract

Introduction: Soil microorganisms are the essential part of forest ecosystems which play a key role on soil nutrient changes. The biological activity in soil is largely concentrated in topsoil. Despite the small volume of microorganisms in soil, they have a key role on nitrogen, sulphur and phosphorous cycles and the decomposition of organic residues. Soil microorganisms have been identified as the sensitive indicators for soil quality. The composition of microorganisms and their fractional activities in soils significantly affect biochemical cycles, carbon sequestration and soil fertility. As soil microbial communities respond differently respected to environmental conditions, it seems that variation in forest ecosystem could significantly affect microbial community. Plants are one of the important variables for assessing soil microbial communities which their effect is related to root secretions and litter decomposition. The phospholipid fatty acid (PLFA) analysis is one of the methods that can overcome the problem of selective growth of microorganisms on culture media which is a major defect in the identification of microbial diversity. The objective of this study was to investigate the effects of different tree compositions and soil properties on soil microbial community using PLFA analysis approach.
Materials and Methods: This study was conducted in ShastKalate forest, an experimental forest station of Gorgan University, located at eastern Caspian region, North of Iran (36° 43′ 27″ N ,54°24′ 57″ E). Eleven different tree compositions were selected and the surface soils collected from 0-10 cm depth of 33 plots. Soil samples were air dried and passed through a 2mm sieve. Then one portion of the sieved samples was used for physical and chemical analyses. The other portion was rewetted to 65% of field capacity and incubated at 37 °C for 3 days to analyses PLFA. Soil particle size distribution (clay, silt and sand) was determined using the hydrometer method. Soil pH in 1/ 2.5 soil to water suspension and electrical conductivity (EC) in the same extract were measured.. Calcium carbonate equivalent (CCE),soil organic carbon (OC) and total nitrogen (TN) was determined, too. Biological analyzes including soil microbial respiration determination and PLFA analysis were carried out. The PLFA detection and quantification were performed with a Hewlett-Packard 5890 Series II gas chromatograph (GC) equipped with an HP Ultra 2 capillary column and a flame ionization detector. The normalized data were employed for Pearson's correlation analysis and ANOVA to determine the effects of soil properties and different tree compositions on soil microbial community.
Results and Discussion: Gram+ and Gram- bacteria were the most microorganisms and protozoa were the least microorganisms in soil samples. The results of the correlation between soil properties and microorganisms showed that OC and TN had significant positive effects on microorganism’s communities. EC was significantly correlated with Arbuscular Mycorrhizal Fungi (AMF), actionbacterial, protozoa and total PLFA. In addition, soil microorganisms and total PLFA were significantly correlated with soil respiration. However, there was no significant correlation between TN and OC with protozoa. The correlations between pH, EC, CCE and sand with protozoa were significantly negative, but in the case of silt, this correlation was significantly positive. Different studies showed that soil organic matter is the main nutrient source for soil microorganisms and soil microorganisms are also the essential part of C and N cycles. The effects of tree compositions on 16:0 10-methyl, 18:2 w6c, 20:2 w6c, 20:3 w6c and 20:4 w6c were significant(p

Keywords

Hornbeam; Maple; Microbial community; Parrotia; Phospholipid fatty acids

References

1. Aislabie J., Deslippe J.R., and Dymond J.R. 2013. Soil microbes and their contribution to soil services. Ecosystem Services in New Zealand - Conditions and Trends, 143–161.

2. Ayres E., Steltzer H., Berg S., Wallenstein M.D., Simmons B.L., and Wall D.H. 2009. Tree species traits influence soil physical, chemical, and biological properties in high elevation forests. PLoS ONE, 4:e5964.

3. Bach E.M., Baer S.G., Meyer C.K., and Six J. 2010. Soil texture affects soil microbial and structural recovery during grassland restoration. Soil Biology and Biochemistry, 42:2182–2191.

4. Bremner J.M., and Mulvaney C.S. 1982. Nitrogen—total. American Society of Agronomy, Soil Science Society of America.

5. Buyer J.S., Roberts D.P., and Russek-Cohen E. 1999. Microbial community structure and function in the spermosphere as affected by soil and seed type. Canadian Journal of Microbiology, 45:138–144.

6. Cusack D.F., Silver W.L., Torn M.S., Burton S.D., and Firestone M.K. 2011. Changes in microbial community characteristics and soil organic matter with nitrogen additions in two tropical forests. Ecology, 92:621–632.

7. Eisenhauer A.N., Beßler H., Engels C., Gleixner G., Habekost M., Milcu A., Partsch S., Sabais A.C.W., Scherber C., Steinbeiss S., Weigelt A., Weisser W.W., and Scheu S. 2010. Plant diversity effects on soil microorganisms support the singular hypothesis. Ecology, 91:485–496.

8. Fang S., Liu D., Tian Y., Deng S., and Shang X. 2013. Tree species composition influences enzyme activities and microbial biomass in the rhizosphere: A rhizobox approach. PLoS ONE, 8:e61461.

9. Gee G.W., and Bauder J.W. 1979. Particle size analysis by hydrometer: A simplified method for routine textural analysis and a sensitivity test of measurement parameters. Soil Science Society of America Journal, 43:1004–1007.

10. Greaves J.E. 1922. Influence of salts on bacterial activities of soil. Botanical Gazette. 73:161–180.

11. Hackl E., Pfeffer M., Donat C., Bachmann G., and Zechmeister-Boltenstern S. 2005. Composition of the microbial communities in the mineral soil under different types of natural forest. Soil Biology and Biochemistry, 37:661–671.

12. Hesse P.R. 1971. A Text Book of Soil Chemistry Analysis. John Murray Ltd. London, 412:120–309.

13. Hoorman J.J. 2011. The role of soil protozoa and nematodes. Fact Sheet: Agriculture and Natural Resources.(Smith KL), The Ohio State University Extension, Colombus, Ohio, 1–5.

14. Jenkinson D.S., and Powlson D.S. 1976. The effects of biocidal treatments on metabolism in soil—V: a method for measuring soil biomass. Soil Biology and Biochemistry, 8:209–213.

15. Jones E.B.G., and Jennings D.H. 1965. The effect of cations on the growth of fungi. New Phytologist, 64:86–100.

16. Koorem K., Gazol A., Opik M., Moora M., Saks U., Uibopuu A., Sober V., and Zobel M. 2014. Soil nutrient content influences the abundance of soil microbes but not plant biomass at the small-scale. PLoS ONE, 9:e91998.

17. Liu D., Liu Y., Fang S., and Tian Y. 2015. Tree species composition influenced microbial diversity and nitrogen availability in rhizosphere soil. Plant Soil and Environment, 61:438–443.

18. Lundquist E.J., Scow K.M., Jackson L.E., Uesugi S.L., and Johnson C.R. 1999. Rapid response of soil microbial communities from conventional, low input, and organic farming systems to a wet/dry cycle. Soil Biology and Biochemistry, 31:1661–1675.

19. Marschner P., Yang C., Lieberei R., and Crowley D.E. 2001. Soil and plant specic effects on bacterial community composition in the rhizosphere. Soil Biology and Biochemistry, 33:1437–1445.

20. Merbach W., Mirus E., Knof G., Remus R., Ruppel S., Russow R., Gransee A., and Schulze J. 1999. Release of carbon and nitrogen compounds by plant roots and their possible ecological importance. Journal of Plant Nutrition and Soil Science, 162:373–383.

21. Miethling R., Wieland G., Backhaus H., and Tebbe C.C. 2000. Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33. Microbial Ecology, 40:43–56.

22. Nguyen C. 2003. Rhizodeposition of organic C by plants: mechanisms and controls. In Agronomie, 23: 375–396.

23. Nielsen M.N., Winding A., Binnerup S., Hansen B.M., Hendriksen N.B.and Kroer N. 2002. Microorganisms as indicators of soil health, Neri. National Environmental Research Institute, Denmark.

24. Rath K.M., Maheshwari A., Bengtson P. and Rousk J. 2016. Comparative toxicity of salts to microbial processes in soil. Applied and Environmental Microbiology. 82:2012–2020.

25. Ronn R., Vestergard M., and Ekelund F. 2012. Interactions between bacteria, protozoa and nematodes in soil. Acta Protozoologica, 51:223–235.

26. Rutherford P.M., and Juma N.G. 1992. Influence of soil texture on protozoa-induced mineralization of bacterial carbon and nitrogen. Canadian Journal of Soil Science. 72:183–200.

27. Schutter M.E., and Dick R.P. 2000. Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities. Soil Science Society of America Journal, 64:1659–1668.

28. Shataee Jouibary S. 2003. Survey possibility forest type map using satellite data the case study nowshahr Khairoud kenar. Forestry Ph.D. Thesis. Natural Resource Faculty of Tehran University, 155p.

29. Shi D., and Wang D. 2005. Effects of various salt-alkaline mixed stresses on Aneurolepidium chinense (Trin.) Kitag. Plant and Soil, 27(1):15–26.

30. Six J., Elliot E.T., and Paustian K. 2000. Soil microaggregate turnover and microaggregate formation: a mechanism for C organic under no-tillage agriculture. Soil Biology and Biochemistry, 32:2099–2103.

31. Stephan A., Meyer A.H., and Schmid B. 2000. Plant diversity affects culturable soil bacteria in experimental grassland communities. Journal of Ecology, 88:988–998.

32. Urbanova M., Snajdr J., Baldrian P., Urbanova M., Snajdr J., and Baldrian P. 2015. Composition of fungal and bacterial communities in forest litter and soil is largely determined by dominant trees. Soil Biology and Biochemistry, 84:53–64.

33. Whitman W.B., Coleman D.C., and Wiebe W.J. 1998. Prokaryotes: the unseen majority. Proceedings of the National Academy of Sciences, 95:6578–6583.

34. Williams M.A., Myrold D.D., and Bottomley P.J. 2006. Carbon flow from 13C-labeled straw and root residues into the phospholipid fatty acids of a soil microbial community under field conditions. Soil Biology and Biochemistry, 38:759–768.

35. Yan N., Marschner P., Cao W., Zuo C., and Qin W. 2015. Influence of salinity and water content on soil microorganisms. International Soil and Water Conservation Research, 3:316–323.

36. Zelles L. 1999. Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of microbial communities in soil: A review. Biology and Fertility of Soils, 111–129.