Effect of Putrescine and Mycorrhiza on Growth, Photosynthesis and Vase Life of Gerbera (Gerbera jamesonii) ‘Dune’ Flowers in Hydroponic Conditions

علوم باغبانی

Article 10, Volume 35, Issue 1 - Serial Number 49, February 2021, Pages 117-133   

Document Type: Research Article

DOI: 10.22067/jhs.2021.61915.0

Authors

Soheila Rakbar¹; Zohreh Jabbarzadeh^{* 1}; Mohsen Barin²

¹Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran

²Department of Soil Scinces, Faculty of Agriculture, Urmia University, Urmia, Iran

Abstract

Introduction: Gerbera is one of the most important cut flowers in the world and belongs to the Asteraceae family. Due to its diverse and adaptable species for growth with a wide range of climatic conditions, this flower has become a profitable cut flower for growers. Polyamines in plant tissues act as a potent factor in preventing the production of ethylene. Polyamines and ethylene have antagonistic effects (anti-aging and aging effects), so that the balance of these two hormonal groups in plants is very important for plant tissues. The balance between the two opposing regulators leads to a delay or acceleration in the aging process. Mycorrhizal fungi can be useful in hydroponic greenhouse systems, which increase the amount of CO₂ in greenhouses by increasing photosynthesis in plants, as well as CO₂ emissions in the control environment, which both optimally manage the environment and increase the yield and quality of plant products. Due to the economic importance of cut flowers, it seems necessary to provide treatments (such as the use of putrescine and mycorrhiza) to increase the quality and longevity of this plant.
Materials and Methods: This study was conducted in the research and production greenhouses of Urmia University and the research laboratory of the Department of Horticultural Sciences of the Faculty of Agriculture in 2019-2020. This study was performed as a factorial experiment in a completely randomized design with three replications, each replication consistedd of three pots and each pot contained a plant. The factors of this experiment were Mycorrhizal fungi inoculation (with and without inoculation) during the transplanting process to the culture medium near the roots, and putrescine at four concentrations of 0 (control), 1, 2, and 4 mM, were applied two weeks after transplantation, every 15 day-interval for three months. In order to investigate the effects of putrescine and mycorrhizal fungi on some morphological and physiological characteristics of plants, two weeks after the end of treatments, leaf sampling was performed to measure physiological characteristics. Effects of putrescine and mycorrhizal fungi were assayed in some morphological characteristics including leaf number, leaf length and leaf area, fresh and dry weight of leaves and some physiological parameters including chlorophyll index, chlorophyll content (a, b and total) and soluble sugar as well as vase life and petal’s anthocyanin during postharvest time. The SAS software version 9.1 was used to analyze the variance and compare the mean of the studied traits. Comparison of means was performed using the Tukey’s range test method at a probability level of 1 and 5%. Excel (2016) software was also used to draw the graph.
Results and Discussion: According to the comparison of means, putrescine, along with mycorrhizal fungi, increased the number of leaves, leaf area, and the fresh and dry weight of the leaves as well as chlorophyll index, chlorophyll a, b and total and carotenoid content of leaves. In this study, inoculation with mycorrhiza reduced leaf length but increased leaf area resulted in that mycorrhiza could increase leaf blade because of increasing cytokinin in plant. Putrescine with mycorrhizal fungi, increased leaf growth, photosynthesis of plant and carbohydrates production. In the literatures, it is reported that, the vase life of cut flowers is a very important point in choosing them as great cut flowers. The results showed, putrescine and mycorrhiza had increased the vase life of gerbera flowers, therefore increased the quality of this plant. Putrescine and mycorrhiza also increased the amounts of anthocyanins in the petals, and by the sixth day, the highest levels of anthocyanins were observed in the petals. Probably, the reason for increasing the anthocyanins on the sixth day is the presence of carbohydrates stored in the flower, which due to the reduced respiration and carbohydrate consumption in this process.
Conclusion: Based on the results of the present study, it can be concluded that putrescine, with mycorrhizae, improved growth characteristics as well as increasing the postharvest life and the quality of cut flowers of gerbera. It is also observed that among the different concentrations of putrescine, the concentration of 2 mM had the greatest effect on the growth and physiological parameters as well as vase life of gerbera.
 

Keywords

Anthocyanin; Chlorophyll; Fresh and dry weight of leaf; Gerbera; Vase life

References

1-       Abbasi N.A., Ali I., Hafiz I.A., and Khan A.S. 2017. Application of polyamines in horticulture: A review. International Journal of Biosciences 10(5): 319-342.

2-       Abdel-Salam E., Alatar A., and El-Sheikh M.A. 2018. Inoculation with arbuscular mycorrhizal fungi alleviates harmful effect of drought stress on damask rose. Saudi Journal of Biological Sciences 25: 1772-1780.

3-       Asghari M.R. 2015. Classic plant growth hormones and regulators. University of Urmia, 352 p. (In Persian).

4-       Asnaashari M., and Zakai Khosroshahi M. 2008. Polyamines and Horticultural Sciences. Publications of Bu Ali Sina University, 163 p. (In Persian).

5-       Badawy E.M., Kandil M.M., Mahgoub M., Shanan N., and Hegazi N. 2015. Chemical constituents of Celosia argentea var. cristata L. plants as affected by foliar application of putrescine and alph-tocopherol. International Journal of Chemical Technology Reaserch 8(12): 464-470.

6-       Barman J., Samanta A., Saha S., and Datta S. 2016. Mycorrhiza. General Article 21: 1093-1104.

7-       Berberich T., Kusano T., Takahashi Y., and Tateda C. 2008. Polyamines: essential factors for growth and survival. Planta 228: 367-381.

8-       Bhat R.A., Dervash M.A., Mehmood M.A., Skinder B.M., Rashid A., Ahmad J.I., Singh B.D.V., and Lone L. 2017. Mycorrhizae: A sustainable industry for plant and soil environment. Springer International Publishing AG, Chapter 25.

9-       Chinnusamy V., Gong Z., and Zhu J.K. 2008. Abscisic acid-mediated epigenetic processes in plant development and stress responses. Journal of Integrative Plant Biology 50: 1187–1195.

10-   Danaee E., and Abdossi V. 2018. Effect of different concentrations and application methods of polyamines (Putrescine, Spermine, Spermidine) on some morphological, physiological, and enzymatic characteristics and vase life of rosa hybrida cv. ‘Dolce Vita’ cut flower. Journal of Ornamental Plants 8(3): 171-182.

11-   Dastyaran M., and Hosseini Farahi M. 2014. Effect of humic acid and putrescine on vegetative and flowering characteristics of roses in soilless system. Science and Technology of Greenhouse Cultures 20: 243-252. (In Persian with English Abstract)

12-   Figueroa I., Colinas M.T., Mejia J., and Remirez Cien F. 2005. Post-harvest physiological changes in roses of different vase life. Cienciae Investigación Agraria 32: 167-176.

13-   Ghaderi Kh., and Nazarideljo M.J. 2017. Morphophysiological changes and quality of Gerbera jamesonii cut flowers under the influence of inoculation of mycorrhizal bed with mycorrhizal fungi in soilless system. Science and Technology of Greenhouse Cultures 8(4):27-39. (In Persian with English Abstract)

14-   Habba E.E., Abdel Aziz N.G., Sarhan A.M.Z., Arafa A.M.S., Youssefi N.M. 2016. Effect of putrescine and growing media on vegetative growth and chemical constituents of Populus euramericana plants. Journal of Innovations in Pharmaceuticals and Biological Sciences 3(1): 61-73.

15-   Haghighi M., Nikbakhat A., and Pessarakli M. 2015. Effects of humic acid on remediation of the nutritional deficiency of Gerbera in hydroponic culture. Journal of Plant Nutrition 39: 702-713.

16-   Hosseini Farahi M., and Aboutalebi Jahroomi A. 2018. Effect of pre-harvest foliar application of polyamines and calcium sulfate on vegetative characteristics and mineral nutrient uptake in Rosa hybrida. Journal of Ornamental Plants 8(4): 241-253.

17-   Irigoyen J.J., Emerich D.W., and Sanchez- Diaz M. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Plant Physiology 84: 55-60.

18-   Kafi M., Daneshvar N., Nikbakht A., Hakimi M., Rajali F., and Daneshkhah M. 2013. Effect of humic acid and mycorrhizal fungus on some characteristics of Lolium Combination of White Speed Green. Science and Technology of Greenhouse Cultures 4(13): 49-58. (In Persian with English Abstract)

19-   Kahrobaiyan M., Nemati S.H., Rahemi M., Kholdebarin B., and Tehranifar A. 2019. Morphological responses of ornamental sunflower to putrescine treatment under drought conditions. Applied Ecology and Environmental Research 17(3): 6117-6127.

20-   Kamiab F., and Zamanibahramabadi E. 2016. The effect of different polyamines on some physiological traits as ACC oxidase and superoxide dismutase enzymes activity in Chrysanthemum morifolium cv. ‘Bright Golden Ann’. Journal of Ornamental Plants 6(2): 83-92.

21-   Kandil M.M., Soad M.M., Ibrahaim S.H., El-Hanafy S.H., and El-Sabwah M.M. 2015. Effect of putrescine and uniconazole on some flowering characteristics and some chemical constituents of  Saliva splendens F. plant.  Journal of Chemical Technology and Biotechnolog 8(9): 178-186.

22-   Khalaj M.A., Kiani Sh., Khoshgoftarmanesh A.H., and Amoaghaie R. 2017. Growth, quality, and physiological characteristics of gerbera (Gerbera jamesonii L.) cut flowers in response to different NO3¯:NH4⁺ ratios. Horticulture, Environment and Biotechnology 58(4): 313-323.

23-   Khoshkhui M., Sheibani B., Rouhani E., and Tafzoli A.A.  2010. Principles of Gardening. Shiraz University Press, 19th Edition, 596 p. (In Persian).

24-   Krizek D.T., Kramer G.F., Upadhyaya A., and Mirecki R.M. 1993. UV B response of cucumber seedlings grown under metal halide and high pressure sodium/deluxe lamps. Physiologia Plantarum 88: 350-358.

25-   Kusano T., and Suzuki H. 2015. Polyamines. Springer, 336p.

26-   Liang F., An J., Gao J.Q., Zhang X.Y., and Yu F.H. 2018. Effects of arbuscular mycorrhizal fungi and soil nutrient addition on the growth of Phragmites australis under different drying-rewetting cycles. PLoS ONE 13(1):1-10.

27-   Lichtenthaler H.K. 1987. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology 148: 350-382.

28-   Mahgoub M., Abdel Aziz N.G., and Mazhar A. 2011. Response of Dahlia pinnata L. plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. American-Eurasian Journal of Agriculture and Environmental Science 10(5): 769-775.

29-   Manoharan P.T., Pandi M., Shanmugaiah V., Gomathinayagam S., and Balasubramanian N. 2008. Effect of vesicular arbuscular mycorrhizal fungus on the physiological and biochemical changes of five different tree seedlings grown under nursery conditions. African Journal of Biotechnology 7: 3431-3436.

30-   Meir D., Pivonia S., Levita R., Dori I., Ganot L., Meir S., Salim S., Resnick N., Wininger S., Shlomo E., and Koltai H. 2010. Application of mycorrhiza to ornamental horticultural crops: lisianthus (Eustoma grandiflorum) as a test case. Spanish Journal of Agricultural Research 8(1): 1-10.

31-   Mishra V., Ellouze W., and Howard R.J. 2018. Utility of arbuscular mycorrhizal fungi for improved production and disease mitigation in organic and hydroponic greenhouse crops. Journal of Horticulture 5 (3):1-10.

32-   Najm A.A., Haj Seyed Hadi M.R., Fazeli F., Darzi M.T., and Rahi A. 2012. Effect of integrated management of nitrogen fertilizer and Cattle manure on the leaf chlorophyll, yield and tuber glycoalkaloids of Agria potato. Communications in Soil Science and Plant Analysis 43: 912-923.

33-   Ramtin A., Jahangiri M., Bagheri M.H., and Ferdowsizadeh M. 2018. Production and cultivation of ornamental plants. Educational Research and Planning Organization, Pp. 75-76.

34-   Rezvanypour S., Hatamzadeh A., Elahinia S.A., and Asghari H.R. 2015. Exogenous polyamines improve mycorrhizal development and growth and flowering of Freesia hybrida. Journal of Horticultural Research 23(2): 17-25.

35-   Rymbai H., Jha A.K., Talang H.D., Assumi S.R., Deshmukh N.A., and Roy A.R. 2017. Evaluation of new gerbera (Gerbera jamesonii Bolus) genotypes under fan and pad polyhouse. Journal of Ornamental Horticulture 20(3&4): 126-131.

36-   Savvas D., and Ntatsi G. 2015. Biostimulant activity of silicon in horticulture. Scientia Horticulturae 196: 66-81.

37-   Setia N., and Setia R.C. 2018. Polyamines: An overview and prospects in crop improvement. Punjab Agricultural University 21: 376-393.

38-   Soleimany-Fard E., Hemmati Kh., and Khalighi A. 2014. Impact of pre- and post-harvest putrescine applications on water relations and vase life of cut alstroemeria flowers. Advances in Environmental Biology 8(12): 158-165.

39-   Taranet P., Harper S., Kirchhof G., Fujinuma R., and Menzies N. 2017. Growth and yield response of glasshouse and field-grown sweetpotato to nitrogen supply. Nutrient Cycling in Agroecosystems 108: 21-309.

40-   Tatte S., Singh A.,  and Ahlawat T.R. 2015. Effect of polyamines on postharvest quality and vaselife of rose var. samurai. The Bioscan 10(2): 675-678.

41-   Tognon G.B., Sanmartín C., Alcolea V., Cuquel F.L., and Goicoechea N. 2015. Mycorrhizal inoculation and/or selenium application affect post-harvest performance of snapdragon flowers. Plant Growth Regulation 78: 389–400.

42-   Vieira M.R.S., Moura F.D.,  Simões N.A., Souza A.V.,. Santos C.M.G., Paes R.A., and Leal H.Y. 2017. Application of polyamine and boron improves quality of potted gerbera cv. ‘‘Kosak’’. Journal of Applied Horticulture 19(1): 1-5.

43-   Vukajovic D., Veghel H., and Durovic S. 2017. Economic justification for floriculture development in Serbia. Economics of Agriculture 64(2):687-699.

44-   Wu Q.S., Srivastava A.K., Cao M.Q., and Wang J. 2015. Mycorrhizal function on soil aggregate stability in root zone and root-free hyphae zone of trifoliate orange. Archives of Agronomy and Soil Science 61(6): 813–825.

45-   Zeraat Kish Y., and Mirzaee N. 2016. A study of the factors affecting the insurance acceptance by ornamental plants producers: A case study of kohgiluyeh and boyer ahmad province. Journal of Ornamental Plants 6(2): 67-72.