Optimization of tissue culture condition in lentil (Lens culinaris Medik. cv. Gachsaran) to induce effective multiple shoot induction

پژوهشهای زراعی ایران

Volume 7, Issue 2 - Serial Number 14, 2009   

Document Type: Research Article

Abstract

Optimization of regeneration in lentil is necessary to improve this plant by novel methods. First stage of regeneration is efficient multiple shoot induction using proper explants. In this research, four explants of leaflet, stem, cotyledon without embryo axes and cotyledon with embryo axes were tested to determine the best explant. Probably effect of BAP in germination media of seedling tested on shoot induction of explants. There was comparison between effect of different level and combination of various cytokinins on multiple shoot induction. Effect of explant age and pH of media also tested on shoot induction. Results showed that, cotyledon with embryo axes is the best explant. Existence of BAP in germination media was very useful. There was highest shoot induction percentage (until to 96%) in MS media complemented with 7.5μM 2ip, 4μM Kin and 2 μM TDZ. Using this media produced more than 40 shoots per explant in some samples. Age of explant and pH of media was effective on multiple shoot induction. Results of primary research in gene transformation of lentil indicated efficient ability of this protocol to use in gene transformation.

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