Functional cloning of resistant genes against late blight in Solanum demissum genotype

پژوهشهای زراعی ایران

Volume 7, Issue 2 - Serial Number 14, 2009   

Document Type: Research Article

Abstract

Demissum differentials are important for pathogen isolates identification. Recent studies show Ma.R9 contains R2 and R3 or homologues of the genes. This genotype has a high resistance against different isolates in the world. Candidate gene approach applied to isolate and cloning of these homologues in this genotype. Phylogenetic clades drawn and expression assay was done by coinfiltration in N. benthamiana. Result revealed this genotype contain R3a and also functional R2 like gene. Alignment of R2 gene against R2-like in amino acids level showed completely conserved residues in LRR region. Presence of more than one gene could be the reason of resistance against different isolates. These results confirm high efficiency of candidate cloning approach and effector genomics as new approaches.

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