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Mehdinejad, F., Zeynadini Riseh, A., Sedaghati, E., Alaei, H., Moradi, M. (2021). Evaluation of the Level of Defense Enzymes Induced by Antagonistic Fungi against Root Knot Nematode, Meloidogyne javanica in Pistachio Seedlings. , 35(1), 25-37. doi: 10.22067/jpp.2021.32842.0
F. Mehdinejad; A. Zeynadini Riseh; E. Sedaghati; H. Alaei; M. Moradi. "Evaluation of the Level of Defense Enzymes Induced by Antagonistic Fungi against Root Knot Nematode, Meloidogyne javanica in Pistachio Seedlings". , 35, 1, 2021, 25-37. doi: 10.22067/jpp.2021.32842.0
Mehdinejad, F., Zeynadini Riseh, A., Sedaghati, E., Alaei, H., Moradi, M. (2021). 'Evaluation of the Level of Defense Enzymes Induced by Antagonistic Fungi against Root Knot Nematode, Meloidogyne javanica in Pistachio Seedlings', , 35(1), pp. 25-37. doi: 10.22067/jpp.2021.32842.0
Mehdinejad, F., Zeynadini Riseh, A., Sedaghati, E., Alaei, H., Moradi, M. Evaluation of the Level of Defense Enzymes Induced by Antagonistic Fungi against Root Knot Nematode, Meloidogyne javanica in Pistachio Seedlings. , 2021; 35(1): 25-37. doi: 10.22067/jpp.2021.32842.0

Evaluation of the Level of Defense Enzymes Induced by Antagonistic Fungi against Root Knot Nematode, Meloidogyne javanica in Pistachio Seedlings

پژوهش های حفاظت گیاهان ایران
Article 3, Volume 35, Issue 1 - Serial Number 51, March 2021, Pages 25-37    PDF (930.26 K)
Document Type: Research Article
DOI: 10.22067/jpp.2021.32842.0
Authors
F. Mehdinejad1; A. Zeynadini Riseh* 1; E. Sedaghati1; H. Alaei1; M. Moradi2
1Faculty of Agriculture, Vali-e-Asr University of Rafsanjan
2Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rafsanjan
Abstract
Introduction: Pistachio is one of the most important export products of the country and root knot nematode is one of the threatening factors in its production. The most prevalent species in pistachio orchards are those of Meloidogyne javanica and M. incognita. Plants have a wide range of defense mechanisms effective against the invasion of various pathogens and pests. These mechanisms include pre-existing physical barriers, chemical barriers, and induced defense responses. Beneficial soil microorganisms such as Trichoderma spp. and arbuscular mycorrhizal fungi (AMF) can protect plants from infection by direct mechanisms such as production of toxins, enzymes, and other metabolites or by inducing systemic resistance. Induce resistance to the root knot nematode occurs following an increased accumulation of different antagonistic compounds such as peroxidase and polyphenol oxidase. Increased levels of these enzymes has been observed in different plants that are responsible for the induced protection against Meloidogyne.
Materials and Methods: In this study potential of antagonistic fungi, vesicular-arbuscular mycorrhiza (Funneliformis mosseae, Rhizophagus intraradices, Funneliformis caledonius) and Trichoderma (Tricoderma aureoviride,Tricoderma harzianum) and mixed of both fungi against Meloidogyne javanica under greenhouse conditions was investigated. In order to investigate the effect of antagonistic fungi on the induction of defense enzymes in pistachio seedlings of Badami Riz Zarand cultivar, the study was performed as a factorial experiment based on completely randomized design with twelve treatments in three replications in the greenhouse. Pistachio seedlings were inoculated at the stage of 8-10 leaf with 60 g of mycorrhizal mixture and three months later, these plants were sampled for examination. In order to ensure the purity of the fungi and the colonization of the roots, staining of the plant roots was performed and microscopic examination was performed to observe different organs of the fungus. After confirming the mycorrhiza treatment, Trichoderma treatment was applied. For Trichoderma treatment, 10 g of mixture of isolates T1, T2, T3, T4 and T5 per kg of soil was used. Pure nematode populations were prepared from a single egg mass on Early Urbana tomatoes. Pistachioseedlings were inoculated with 5000 second-stage juveniles of nematode 21 days after Trichoderma treatment.Nematode indices including the number of galls, egg masses and second juveniles per gram of root, the number of eggs in each egg mass and reproductive factor after 75 days for each treatment were measured.The second experiment was performed to investigate the effect of biological treatments on nematode indices in a completely randomized design with six treatments and three replications. Defense enzymes level of Peroxidase (POX), Polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) determined at four times of one and a half, three, five and a half and twelve days after nematode inoculation.
Results and Discussion: Disease indices showed a significant decrease in fungi treatments compared to the nematode control. Trichoderma treatment showed a significant difference in all indicators compared to the other treatments. Reproductive index in Trichoderma treatment and its combination with mycorrhizal treatment decreased by 72 and 33.3 and nematode population decreased by 73.4% and 36.2% compared to the control, respectively. Extracellular hydrolytic enzymes play important role in the infection process of Tichoderma species against plant-parasitic nematodes. In mycorrhiza treatment, although there was no significant difference in gall index and egg mass with the control, but the number of second juveniles and nematode reproductive factor showed a significant decrease compared to the control. This may be due to the small size of the egg mass and the effect on the number of eggs. Previous results on mycorrhizal species of Rizophagus irregularis in tomato plant showed that egg masses and number of eggs in treated roots have a significant reduction. The maximum enzymatic activity for POX, and PPO was obtained in different treatments on the twelfth day. The maximum amount for PAL activity in seedlings treated with mycorrhizal and Tricoderma fungi was observed after five and a half days. The level of different enzymes increases after treatment with mycorrhiza and Trichoderma in plants, each of which plays a role in limiting the development of nematodes.
Conclusion:Due to the high proliferation of nematodes in pistachio orchards and due to the environmental hazards of nematicides, the use of biocontrol agents is recommended. The studied fungal strains in the present study could be used as components in an integrated approach to manage M. incognita on pistachio plants. The use of plant growth promoting fungi such as mycorrhiza and Trichoderma while improving plant growth, can reduce the use of fertilizers and chemical pesticides and are effective for better plant growth and development and disease control. However, further studies on the use of combinations of these fungi with other bacterial antagonists and even animal manures as well as garden studies are necessary.
 
Keywords
Biological control; Mycorrhiza; Pistachio; Trichoderma
References
  1. Akbar N.S., and Nadeem S. 2014. Simulation of peristaltic flow of chyme in small intestine for couple stress fluid. Meccanica 49(2): 325-334.
  2. Aliramaji F., Pourjam E., and Karegar A. 2005. Some tylenchids associated with pistachio and almond trees in Iran. In: IV International symposium on pistachios and almonds, Acta Horticulturae 726: 659-666.
  3. AL-Shammari T.A., Bahkali A.H., Elgorban A.M., El-Kahky M.T., and Al-Sum B.A. 2013. The use of Trichoderma longibrachiatum and Mortierella alpina against root-knot nematode, Meloidogyne javanica on tomato. Journal of Pure and Applied Microbiology 7: 199-207.
  4. Anita B., and Samiyappan R. 2012. Induction of systemic resistance in rice by Pseudomonas fluorescens against rice root knot nematode Meloidogyne graminicola. Journal of Biopesticides5: 53-59.
  5. Anjos É.C.T.D., Cavalcante U.M.T., Gonçalves D.M.C., Pedrosa E.M.R., Santos V.F.D., and Maia L.C. 2010. Interactions between an arbuscular mycorrhizal fungus (Scutellospora heterogama) and the root-knot nematode (Meloidogyne incognita) on sweet passion fruit (Passiflora alata). Brazilian Archives of Biology and Technology 53(4): 801-809.
  6. Annapurna M., Bhagawati B., and Kurulkar U. 2018. Biochemical Mechanism of Native Fungal Bioagents in the Management of Root-Knot Nematode Meloidogyne incognita on Tomato. International Journal of Current Microbiology and Applied Science 7(11): 380-395.
  7. Barea J.M., Azcón R., and Azcón-Aguilar C. 2002. Mycorrhizosphere interactions to improve plant fitness and soil quality. Antonie van leeuwenhoek 81(1-4): 343-351.‏
  8. Barker K.R., and Koenning S.R. 1998. Developing sustanaible system for nematode management. Annual Review of Phytopathology 36(1): 165-205.‏
  9. Beckman T.G., and Pusey P.L. 2001. Field Testing peach rootstocks for resistance to Armillaria root rot. HortScience 36(1): 101-103.
  10. D'cunha G.B., Satyanarayan V., and Nair P.M. 1996. Purification of phenylalanine ammonia lyase from Rhodotorula glutinis. Phytochemistry 42(1): 17-20.‏
  11. Deepak S., Niranjan-Raj S., Shailasree S., Kini R.K., Boland W., Shetty H.S., and Mithöfer A. 2007. Induction of resistance against downy mildew pathogen in pearl millet by a synthetic jasmonate analogon. Physiological and Molecular Plant Pathology 71(1-3): 96-105.‏
  12. Dong H., Li W., Zhang D., and Tang W. 2003. Differential expression of induced resistance by an aqueous extract of killed Penicillium chrysogenum against Verticilliumwilt of cotton. Crop Protection 22(1): 129-134.
  13. Duncan L.W., and Ferris H. 1983. Validation of a model for prediction of host damage by twonematode species. Journal of Nematology 15: 227-234.
  14. Edreva A., Velikova V., Tsonev T., Dagnon S., Gürel A., Aktaş L., and Gesheva E. 2008. Stress-protective role of secondary metabolites: diversity of functions and mechanisms. General andAppliedPlantPhysiology 34(1-2): 67-78.‏
  15. Esfahani L., Jamali S., Saeedizadeh A., and Pedramfar H. 2017. Inducing systemic resistance of tomato by salicylic acid and two - biocontrol agents against root- knot nematode. Iranian Journal of Plant Protection Science 48(2): 283-294. (In Persian with English abstract)
  16. Farivar Mehin H. 1984. Study of the Root-knot Nematodes on Pistachio in Iran. In:Proceeding of the first International Congress of Nematology, 5-10 Aug, Guelph, Ontario, Canada. 26-27.
  17. Fatemy S. 2009.  Integrated management of pistachio nematodes. P. 243-252.In Ciancio A. Mukerji, K.G. (ed.) Integrated Management of Fruit Crops and Forest Nematodes. Springer the Netherlands.
  18. Hagn A., Pritsch K., Schloter M., and Munch J.C. 2003. Fungal diversity in agricultural soil under different farming management systems, with special reference to biocontrol strains of Trichoderma spp. Biology and Fertility of Soils 38(4): 236-244.‏
  19. He C.Y., Hsiang T., and Wolyn D.J. 2002. Induction of systemic disease resistance and pathogen defense responses in Asparagus officinalis inoculated with nonpathogenic strains of Fusarium oxysporum. Plant Pathology 51(2): 225-230.‏
  20. Hussey R.S., and Barker K.R. 1973. A Comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant disease Report57:1025-1028.
  21. Jebeli ameli F. 2006. Factors affecting export of pistachio saffron and dates in the non-oil commodities basket. Journal of Agriculture and Development, 14(54): 85-102. (In Persian)
  22. Jepson S.B. 1987. Identification of root-knot nematodes (Meloidogyne species). CAB international, Wallingford, UK.
  23. Khatamidoost Z., Jamali S., Moradi M., and Saberi-Riseh R. 2015. Effect of Iranian Strains of Pseudomonas spp. On the Control of Root-Knot Nematodes on Pistachios. Biocontrol Science Technology, 25(3): 291-301.
  24. Liu R., Dai M., Wu X., Li M., and Liu X. 2012. Suppression of the root-knot nematode [Meloidogyne incognita (Kofoid and White) Chitwood] on tomato by dual inoculation with arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria. Mycorrhiza 22(4): 289-296.‏
  25. Manandhar S. 2011. Study on the bioprotective effect of endomycorrhizae against M. graminicola in rice. Journal of Biological Pest Control 5(1): 28-35.
  26. Martínez-Medina A., Roldán, A., Albacete A., and Pascual J. A. 2011. The interaction with arbuscular mycorrhizal fungi or Trichoderma harzianum alters the shoot hormonal profile in melon plants. Phytochemistry 72(2-3): 223-229.
  27. Milavec M., Gruden K., Ravnikar M., and Kovač M. 2008. Peroxidases in the early responses of different potato cultivars to infection by Potato virus YNTN. Plant Pathology 57(5): 861-869.‏
  28. Nagesh M., and Reddy P.P. 2004. Biochemical changes in Glomus fasciculatum colonized roots of Lycopersicon esculentum in presence of Meloidogyne incognita. Indian Journal of Experimental Biology 42: 721-727.
  29. Nicoli M.C., Elizable B.E., Piotti A., and Lerici C.R. 1991. Effect of sugar and maillard reaction products on polyphenol oxidase and peroxidase activity in food. Journal of Food Biochemistry 15:169-184.
  30. Odeyemi I.S., Afolami1 S.O., and Sosanya O.S. 2010. Effect of Glomus mosseae (arbucular mycorrhizal fungus) on host parasite relationship of Meloidogyne incognita on four improved cowpea varieties. Journal of Plant Protection Research 50(3): 320-325.
  31. Phillips J.M., and Hayman D.S. 1970. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British mycological Society 55(1): 158-161.
  32. Pieterse J.N. 2001. Hybridity, so what? Department of Sociology of University of Illinois at Urbana-Champaign.
  33. Plewa M.J., Smith S.R., and Wanger E.D. 1991. Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutatation Research 247: 57–64.
  34. Reuveni R., Agapov V., and Reuveni M. 1994. Foliar spray of phosphates induces growth increase and systemic resistance to Puccinia sorghi in maize. Plant Pathology 43(2): 245–250.
  35. Sahebani N., and Hadavi N. 2008. Study on the Systemic Effect of Root-knot Nematode (Meloidogyne javanica) on Phenylalanine Ammonia Lyase Enzyme Activity in Tomato Root in the Interaction between Root-Knot Nematode and Tomato Fusarium Wilt (Fusarium oxysporum f. sp. lycopersici). Isfahan University of Technology-Journal of Crop Production and Processing, 12(43), 217-225.‏ (In Persian)
  36. Sahebani, N., and Hadavi, N. 2008. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Soil Biology and Biochemistry 40(8): 2016-2020.
  37. Schüßler A., and Walker C. 2010. The Glomeromycota: a species list with new families and new genera. The Royal Botanic Garden Kew, Botanische Staatssammlung Munich, and Oregon State University, 19.
  38. Sharma I.P., and Sharma A.K. 2017. Physiological and biochemical changes in tomato cultivar PT-3 with dual inoculation of mycorrhiza and PGPR against root-knot nematode. Symbiosis 71(3): 175-183.
  39. Sharon E., Bar-Eyal M., Chet I., Herrera-Estrella A., Kleifeld O., and Spiegel Y. 2001. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology 91(7): 687-693.
  40. Siddiqui Z.A., and Akhtar M.S. 2009. Effects of antagonistic fungi, plant growth-promoting rhizobacteria, and arbuscular mycorrhizal fungi alone and in combination on the reproduction of Meloidogyne incognita and growth of tomato. Journal of General Plant Pathology 75(2): 144-153.
  41. Sohrabi F., Sheikholeslami M., Heydari R., Rezaee S., and Sharifi R. 2020. Investigating the effect of Glomus mosseae, Bacillus subtilis and Trichoderma harzianum on plant growth and controlling Meloidogyne javanica in tomato. Indian Phytopathology1(3): 1-8.
  42. Taylor A., and Sasser J. 1978. Biology, identification and control of root-knot nematodes(Meloidogyne species). North Carolina State University Graphics, 111.
  43. Van Wees S.C., Van der Ent S., and Pieterse C.M. 2008. Plant Immune Responses Triggered by Beneficial Microbes. Curr. Opin. Plant Boil 11(4): 443-448.
  44. Vos C., Schouteden N., Van Tuinen D., Chatagnier O., Elsen A., De Waele D., and Gianinazzi-Pearson V. 2013. Mycorrhiza-induced resistance against the root–knot nematode Meloidogyne incognita involves priming of defense gene responses in tomato. Soil Biology and Biochemistry 60(1): 45-54.
  45. Whitehead A.G., and Hemming J.R., 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of applied Biology 55: 25-38.
  46. Zhang S.h., Gan Y., and Xu B. 2014. Efficacy of Trichoderma longibrachiatum in the control of Heterodera avenae. BioControl 59(3): 319-331.
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