Identification of Bacterial Proteins in the Gut of Sunn pest, Eurygaster integriceps

Journal of Cell and Molecular Research

مقاله 6، دوره 12، شماره 2 - شماره پیاپی 24، بهار و تابستان 2021، صفحه 97-103  

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.2021.64305.0

نویسندگان

Mohammad Saadati ¹؛ Mahmoud Toorchi²

¹Faculty of Agriculture, University of Birjand, Birjand, Iran

²Faculty of Agriculture, University of Tabriz, Tabriz, Iran

چکیده

     Proteomics is a powerful tool to identify effective proteins in the biochemical reactions of the insect body. Many proteins were reported in the gut lumen and tissues which are essential to complete the physiological role of the alimentary canal of Sunn pest. The gut microbiome of insects has a key role in the digestive process. In this study, for the first time, gut proteins of adult Sunn pest were extracted. These proteins were visualized and identified with two-dimensional polyacrylamide gel electrophoresis and mass spectrometry, respectively. Newly identified proteins include pyruvate dehydrogenase, oxidoreductase FAD-binding protein, hypothetical proteins, glycerol-3 phosphate dehydrogenase, conserved hypothetical proteins, ABC excinucleases, ABC-type transport systems and molecular chaperones. The accumulation of these metabolism proteins in the gut of Sunn pest indicates the importance of symbiotic proteins in the improvement of digestive activities and insect-bacteria interactions. Results suggest that the identified bacterial proteins can be considered as effective proteins in the process of nutrition and provide more gut-derived targets for enzyme engineering and development of biopesticides.

کلیدواژه‌ها

Proteomics؛ Digestive system؛ Scutelleridae؛ Biochemistry؛ Bacteria؛ Two dimensional gel electrophoresis

مراجع

Babbitt P.C., Hasson M.S., Wedekind J. E., Palmer D. R. J., Barrett W. C., Reed G. H. and et al. (1996) The enolase superfamily: A general strategy for enzyme catalyzed abstraction of the α-protons of carboxylic acids. Biochemistry 35:16489-16501.

Barbehenn R. V. (2002) Gut-based antioxidant enzymes in a polyphagous and a graminivorous  grasshopper. Journal of Chemical Ecology 28: 1329-1347.

Baumann P. (2005) Biology bacteriocyte-associated endosymbionts of plant sap-sucking insects. Annual Review of Microbiology 59: 155-189.

Boyd D. W. (2003) Digestive enzymes and stylet morphology of Deraecoris nigritulus (Uhler) (Hemiptera: Miridae) reflect adaptions for predatory habits. Annual Entomology Society America 96: 667-671.

Chandra S. B. C., Vik J. L. and Kapatral V. (2006) Comparative insect mithocondrial genomes: Differences despite conserved genome synthenty. African Journal of Biotechnology 5: 1308-1318.

Chen S., Dong Z., Ren X, Zhao D., Zhang Y., Tang M. and et al. (2019) Proteomic Identification of Immune-Related Silkworm Proteins Involved in the Response to Bacterial Infection, Journal of Insect Science 19: 38-51.

Critchley B. R. (1998) Literature review of sunn pest Eurygaster integriceps Put. (Hemiptera, Scutelleridae). Crop Protection 17: 271-287.

Demandel S., Lin B., Shi M., Li Y., Xu X. and Jin F. (2020) iTRAQ-Based comparative proteomic analysis of larval midgut from the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) challenged with the entomopathogenic bacteria Serratia marcescens. Frontiers in Physiology 11:442-454.

Dillon R. J., Dillon V. M. (2004) The gut bacteria of insects: nonpathogenic interactions. Annual Review Entomology 49: 71-92.

Engel P., Moran N. A. (2013) The gut microbiota of insects – diversity in structure and function. Microbiology Review 37: 699-735.

Fransichetii I. B., Lopes A. H, Dias F. A, Pham V.M. and Ribeiro J. M. (2007) An insight into the sialotranscriptome of the seed-feeding bug, Oncupeltus fasciatus. Insect Biochemistry and Molecular Biology 37: 903-910.

Habibi J., Courdon T. A, Backus E. A., Brandt S. L, Wagner R M., Wright M. K., et al. (2008). Morphology and histology of the alimentary canal of Lygus hesperus (Heteroptera:  Cimicomorpha: Miridae). Annual Entomology Society of America 101: 159-171.

Hou Y., Zou Y., Wang F., Gong J., Zhong X., Xia .Q. and Zhao P. (2010) Comparative analysis of proteome maps of silkworm hemolymph during different developmental stages. Proteome Science 8: 45- 55.

Javaheri M., Scheafer C. W., Lattin J.D. (2009) Shield bugs (Scutelleridae). In: Scheafer C, Panizzi W. (Eds). Heteroptera of economic importance. CRC Press, Washington, USA pp 457-503.

Jouanian L., Bonade-Bottino M., Giard C., Morrot G. and Giband M. (1998) Transgenic plants for insect resistance. Plant Science 131: 1-11.

Kunieda T., Fujiyuki T., Kucharski R., Foret S., Ament S. A., Toth A.L., et al. (2006) Carbohydrate metabolism genes and pathways in insects: insights from the honey bee genome. Insect of Molecular Biology 15: 563-576.

Liu J., Zheng,S., Liu L., Li L. and Feng Q. (2009) Protein profiles of the midgut of Spodoptera litura at the sixth instars feeding stage by shutgun ESI-MS approach. Journal of Proteome Research 2010: 2117-2142.

Mahroof R., Zhu K. H., Neven L., Subramanum B. and Bai J. (2005) Experssion patterns of three heat shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae). Comparative Biochemistry and Physiology 141: 247-256.

Pauchet Y., Muck A., Heckel D.G. and Preiss S. (2008) Mapping the larval midgut lumen proteome of Helicoverpa armigera, a generalist herbivourous insect. Journal of Proteome Research, 7:1629-1639.

Prado S. S., Rubinoff D. and Almeida R. P. P. (2006) Vertical transmission of a pentatomid caeca-associated symbiont. Annual Entomolgical Society of America 99: 577–585

Saadati M. and Toorchi M. (2017) The study of plant protein accumulation in gut of insect using proteomics technique: Wheat–sunn pest interaction. Journal of the Saudi Society of Agricultural Sciences 16: 205-209

Saadati Bezdi M., Farshbaf Pourabad R., Toorchi M., Zarghami N. and Komatsu S. (2012a) Protein patterns in salivary gland of sunn pest, Eurygaster integriceps (put.) (Hem: Scutelleridae). Turkish Journal of Entomology 36: 71-80.

Saadati Bezdi M., Toorchi M., Farshbaf Pourabad R. and Zarghami N. (2012b) Protein map of gut in adult sunn pest, Eurygaster integriceps (Put.) (Hem: Scutelleridae): Two-dimensional electrophoresis technique. Munis Entomology and Zoology 7: 229-237.

Saadati Bezdi M., Toorchi M., Farshbaf Pourabad R., Zarghami N., Nouri M. Z. and Komatsu S. (2012c) Proteome analysis of gut and salivary gland proteins of fifth-instar nymphs and adults of the sunn pest. Archives of Insect Biochemistry and Physiology 81: 105-119.

 Saxena K. N. (1963) Mode of ingestion in a heteropterous insect Dysdercus Koenigii (F.)(Pyrrohocoridae). Journal of Insect Physiology 9: 47-71.

Tunaz H. and Stanley D.W. (2004) Phospholipase A2 in salivary glands isolated from tobacco hornworms, Manduca sexta. Comparative Biochemistry and Physiology, 139: 27-33.

Wilkins M., Pasquali C., Appel R., Ou K., Golaz O., Sanchez J., et al. (1996) From proteins to proteomes: large scale protein identification by 2-D electrophoresis and amino acids analysis. Bio/techniques 14: 61–65.