The Effect of Potassium Foliar Application on Cucumber Plants of ‘Miran’ Cultivar under Drought Stress

علوم باغبانی

Article 2, Volume 36, Issue 3 - Serial Number 55, November 2022, Pages 563-576   

Document Type: Research Article

DOI: 10.22067/jhs.2021.67249.0

Authors

M. Sajedimehr^{* 1}; M. Haghighi²; M. Mehnatkesh²

¹Department of Horticulture . College of Agriculture, Isfahan University of Technology, Isfahan, Iran

²Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

Abstract

Introduction
Drought stress is one of the most important factors limiting plant growth and production and leads to a reduction of more than 50% in the average production of most crops worldwide. Drought stress due to increased soil osmotic potential, especially in greenhouses where fertilizer consumption is high, is one of the greenhouse crop problems.
Material and Methods
In the present study, two concentrations of polyethylene glycol at three levels of 0 (D1), -1.48 (D2), 4-91 (D3 ds/m to create different levels of drought stress due to osmotic changes in culture medium and application of KCl two levels (0 (K1) and 6 (K2) mmol / l) was used to reduce the possible effects. The experiment was performed as a factorial experiment in a completely randomized design with three replications. To apply drought stress, pot water was measured using a tensiometer, and when the drought reached below the field capacity (FC), irrigation with different concentrations of PEG and once a week spraying with KCl at the desired concentrations was done. Cucumber seeds were planted directly in 5 kg plastic pots containing a mixture of potting soil, including soil + sand + animal manure in the ratio of 1 + 2 + 0.5. The number of seedlings in each pot was 2 to 3 kg, which was reduced to one seedling seventeen days after sowing the seeds in the stage of three to four leaves. KCl spraying and spraying began in the three to the four-leaf stage of the seedlings and lasted for about a month. The plants were kept in the greenhouse during the experiment with an average temperature of 25 ° C and relative humidity of 70%. At the end of the experiment, dry weight, fresh weight, chlorophyll, chlorophyll fluorescence, flavonoids, carotenoids, proline, phenol, total protein, abscisic acid, superoxide, and ascorbate peroxidase, antioxidants, and catalase were measured.
Result
The results showed that the effect of foliar application of potassium in all traits except chlorophyll fluorescence and superoxide dismutase was significant (P <0.01). According to the obtained results, ccontrol treatment increased the amount of antioxidants and catalase, but the application of K2 on most of the measured parameters, including dry weight, fresh weight, chlorophyll, flavonoids, carotenoids, proline, phenol, total protein, abscisic acid, and superoxide disodium showed a positive effect. In D3 with the addition of K2 the highest amount of phenol and protein was observed. Also, the content of abscisic acid in all treatments increased with the addition of K2 and the highest amount was observed in D3 which can be concluded that the use of potassium at a concentration of 6 mM Acceptable cut. According to the results obtained in this study, it can be stated that the plant tries to maintain its osmotic pressure in the face of drought stress, and this is done by increasing osmolites such as proline and antioxidant enzymes that help maintain plant cell pressure and torsion. Potassium application can reduce the adverse effects of drought stress by improving the activity of antioxidant enzymes and preserving chlorophyll. Thus, the cell continues its vital activities and ultimately produces more acceptable performance under these conditions. In other words, increasing the antioxidant activity in drought conditions along with the application of potassium leads to a higher inhibitory capacity of reactive oxygen species and production stability in these conditions. Therefore, to compensate for at least some harmful effects of stress and help the plant to return to normal growth conditions after re-irrigation, foliar application of such elements can be effective in drought resistance of the plant and play a role. Based on the findings of this study, it seems that the application of potassium with a concentration of 6 mM is the most effective.

Keywords

Antioxidant enzymes; Foliar application; Osmotic pressure; Polyethylene glycol; Stress

References

• Ahmad, Z., Anjum, S., Waraich, E.A., Ayub, M.A., Ahmad, T., Tariq, R.M.S., Ahmad, R., & Iqbal, M.A. (2018). Growth, physiology, and biochemical activities of plant responses with foliar potassium application under drought stress–a review. Journal of Plant Nutrition 41(13): 1734-1743. https://doi.org/10.1080/01904167.2018.1459688.
• Anjum, S.A., Xie, X.Y., Wang, L.C., Saleem, M.F., Man, C., & Lei, W. (2011). Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research 6(9): 2026-2032. https://doi.org/10.5897/AJAR10.027.
• Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24: 1-15.
• Askari, E., & Ehsanzadeh, P. (2015). Drought stress mitigation by foliar application of salicylic acid and their interactive effects on physiological characteristics of fennel (Foeniculum vulgare ) genotypes. Acta Physiologiae Plantarum 37-4(2): 6. https://doi.org/10.1007/s11738-014-1762-y.
• Barker, A.V., & Pilbeam, D.J. (2007). Handbook of plant nutrition. CRS Press, Taylor and Francis Group. Boca Raton.
• Bates, L.S., Waldren R.P., & Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39(1): 205-207. https://doi.org/10.1007/BF00018060.
• Bilger, W., & Björkman, O. (1994). Relationships among violaxanthin deepoxidation, thylakoid membrane onformation, and nonphotochemical chlorophyll fluorescence quenching in leaves of cotton (Gossypium hirsutum ). Planta 193(2): 238-246. https://doi.org/10.1007/BF00192536.
• Bahrami-Rad, S., & Hajiboland, R. (2017). Effect of potassium application in drought-stressed tobacco (Nicotiana rustica ) plants. Comparison of root with foliar application. Annals of Agricultural Sciences 62(2): 121-130. https://doi.org/10.1016/j.aoas.2017.08.001.
• Boscaiu, M., Sánchez, M., Bautista, I., Donat, P., Lidón, A., Llinares, J., Llul C., Mayoral, O., & Vicentem O. (2010). Phenolic compounds as stress markers in plants from gypsum habitats. Horticulture 67(1): 44-49.
• Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2): 248-254. https://doi.org/10.1016/0003-2697(76)90527-3.
• Daneshian, J., Hrvan, A. M., & Jonoubi, P. (2002). The effect of drought stress and different amounts of potassium on quantitative and qualitative characteristics of soybean. Journal of Agriculture Science 8(1): 95-108.
• Dehqanzadeh, H., Khajehpour, M.R., Heidari Sharif Abad, H., & Soleimani, A.S. (2008). Effect of limited irrigation on the accumulation of proline, free soluble sugars and potassium in bread wheat cultivars. In 10^th Iranian Congress of Agronomy and Plant Breeding Sciences.
• Demiral, & Turkan, I. (2005). Comparative peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Btany 53: 247-257. https://doi.org/10.1016/j.envexpbot.2004.03.017
• Farooq, M., Wahid, A., Kobayashi, N., Fujita, D.B., & Basra, S.M.A. (2009). Plant drought stress: effects, mechanisms and management. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2666-8_12.
• Guo, Z., Ou, W., Lu, S., & Zhong, Q. (2006). Differential responses of Antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiology and Biochemistry 44: 828-836. https://doi.org/10.1016/j.plaphy.2006.10.024.
• HarmantoM., Salokhe M.S., and Babel H.J. (2005). Water requirement of drip irrigated tomatoes grown in greenhouse in tropical environment. Agricultural Water Management 71: 225-242. https://doi.org/10.1016/j.agwat.2004.09.003.
• Hamada, A.M., & EL-enany, A.E. (1994). Effect of NaCl salinity on growth, pigment and mineral element contents, and gas exchange of broad bean and pea plants. Biologia Plantarum 36: 75-81. https://doi.org/10.1007/BF02921273.
• Hasandokht, M. (2005). (Greenhouse Management) Greenhouse production technology. Knowledge Boundary Publications. Tehran. (In Persian)
• Hassani, A., Omid Beigi, R., & Heidari Sharifabad, H. (2003). The effect of different levels of soil moisture on growth, yield and accumulation compatibility metabolites in basil. Journal of Soil and Water Sciences 17(2): 210-219.
• Hura, T., Grzesiak, S., Hura, K., Thiemt, E., Tokarz, K., & Wędzony, M. (2007). Physiological and biochemical tools useful in drought-tolerance detection in genotypes of winter triticale: accumulation of ferulic acid correlates with drought tolerance. Annals of Botany 100(4): 767-775. https://doi.org/10.1093/aob/mcm162.
• Jaleel, C.A., Paramasivam, A., Wahid, M. Farooq, H.J., Al-Juburi, F., & Panneerselvam, R. (2009). Drought stress in plants: A review on morphological characteristics and pigment compositions. International Journal of Agriculture and Biology 11: 100–105. https://doi.org/ 08–305/IGC-DYT/2009/11–1–100–105.
• Kaya, C., Kirnak, H., & Higgs, D. (2001). Effects of supplementary potassium and phosphorus on physiological development and mineral nutrition of cucumber and pepper cultivars grown at high salinity (NaCl). Journal of Plant Nutrition 24(9): 1457-1471. https://doi.org/10.1081/PLN-100106995.
• Khosravi-Far, S., Yarnia, M., Khorshidi Benam, M.B., & Hossein-Zade Moghbeli, A.H. (2008). Effect of potassium on drought tolerance in potato variety Agria. In: Proc 10^th Agron and Plants Breed Cong, 358p. (In Persian)
• Lata, C., Jha, S., Dixit, V., Sreenivasulu, N., & Prasad, M. (2011). Differential antioxidative responses to dehydration-induced oxidative stress in core set of foxtail millet cultivars (Setaria italica ). Protoplasma 248(4): 817-828. https://doi.org/10.1007/s00709-010-0257-y.
• Lin, K.H., Chao, P.Y., Yang, C.M., Cheng, W.C., Lo, H.F., & Chang, T.R. (2006). The effects of flooding and drought stresses on the antioxidant constituents in sweet potato leaves. Botanical Studies 47(4): 417-426.
• Mao, X., Liu, M., Wang, X., Liu, C., Hou, Z., & Shi, J. (2003). Effects of deficit irrigation on yield and water use of greenhouse grown cucumber in the North China Plain. Agricultural Water Management 61: 219-228. https://doi.org/10.1016/S0378-3774(03)00022-2.
• McAdam, S.A., & Brodribb, T.J. (2016). Linking turgor with ABA biosynthesis: implications for stomatal responses to vapor pressure deficit across land plants. Plant Physiology 171(3): 2008-2016. https://doi.org/10.1104/pp.16.00380.
• Mohammadi, A., & Omid, M. (2010). Economical analysis and relation between energy inputs and yield of greenhouse cucumber production in Iran. Applied Energy 87(1): 191-196. https://doi.org/10.1016/j.apenergy.2009.07.021.
• Moradi, F., & Abdelbdghi, M.I. (2007). Responses of photosynthesis, cholorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Annals of Botany 99: 1161-1173. https://doi.org/10.1093/aob/mcm052.
• Moran, J.F., Becana, M., Ormaetxe, I.I., Frechilla, S.L., Klucasc, R.V., & Tejo, D.A. (1994). Drought induces oxidative stress in pea plants. Planta 194: 346-352. https://doi.org/10.1007/BF00197534.
• Movahhedi Dehnavi, M., Modarres Sanavi, A.M., Soroush-Zade, A., & Jalali, M. (2004). Changes of proline, total soluble sugars, chlorophyll (SPAD) content and chlorophyll fluorescence in safflower varieties under drought stress and foliar application of zinc and maganese. Biaban 9(1): 93-110.
• Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22: 867-880. https://doi.org/10.1093/oxfordjournals.pcp.a076232.
• Pennycooke, J.C., Cox, S., & Stushnoff, C. (2005). Relationship of cold acclimation, total phenolic content and antioxidant capacity with chilling tolerance in petunia (Petunia× hybrida). Journal of Experimental Botany 53: 225-232. https://doi.org/10.1016/j.envexpbot.2004.04.002.
• Rauf, M., Munir, M., Hassan, M., Ahmad, M., & Afzal, M. (2007). Performance of wheat genotypes under osmotic stress at germination and early seedling growth stage. African Journal of Biotechnology 6(8): 68-73.
• Rossi, L., Francini, A., Minnocci, A., & Sebastiani, L. (2015). Salt stress modifies apoplastic barriers in olive (Olea europaea ): a comparison between a salt-tolerant and a salt-sensitive cultivar. Scientia Horticulturae 192: 38-46. https://doi.org/10.1016/j.scienta.2015.05.023.
• Sánchez, F.J., Manzanares, M., de Andres, E.F., Tenorio, J.L., & Ayerbe, L. (1998). Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crops Research 59(3): 225-235. https://doi.org/10.1016/S0378-4290(98)00125-7.
• Sangtarash, M.H., Qaderi, M.M., Chinnappa, C.C., & Reid, D.M. (2009). Carotenoid differential sensitivity of canola (Brassica napus) seedlings to ultraviolet-B radiation, water stress and abscisic acid. Environmental and Experimental Botany 66(2): 212-219. https://doi.org/10.1016/j.envexpbot.2009.03.004.
• Shah, T., Khan, A.Z., Numan, M., Ahmad, W., Zahoor, M., Ullah, M., & Jalal, A., (2017). Nutrient uptake and yield of wheat varieties as influenced by foliar potassium under drought condition. Cercetari Agronomice in Moldova 50(2): 5-20. https://doi.org/10.1515/cerce-2017-0011.
• Shahzad, A.N., Fatima, N., Sarwar, S., Bashir, M., Rizwan, M.F., Qayyum, M.K., Qureshi, M., Javaid, H., & Ahmad, S. (2017). Foliar application of potassium sulfate partially alleviates pre-anthesis drought-onduced kernel abortion in maize. International Journal of Agriculture and Biology 19(3):495–501.
• Smirnoff, N. (1993). The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist 27-58-62. https://doi.org/10.1111/j.1469-8137.1993.tb03863.x.
• Soheili Movahed, S., Esmaeeli, A., Jabari, F., & Fooladi, A. (2017). Evaluation of yield and yield Components of some pinto bean (Phaseolus vulgaris) genotypes under late season water deficit conditions, Journal of Agroecology 9(2): 433-444. (In Persian with English abstract)
• Stepien, P., & Klobus, G. (2005). Antioxidant defense in the leaves of C3 and C4 plants under salinity stress. Plant Physiology 125: 31-40. https://doi.org/10.1111/j.1399-3054.2005.00534.x.
• Tabatabaei, S., & Ehsanzadeh, P. (2016). Photosynthetic pigments, ionic and antioxidative behaviour of hulled tetraploid wheat in response to NaCl. Photosynthetica 54: 340-350. https://doi.org/10.1007/s11099-016-0083-3.
• Thalooth, A.T., Tawfik, M.M., & Mohamed, H.M. (2006). A comparative study on the effect of foliar application of zinc, potassium and magnesium on growth, yield and some chemical constituents of mungbean plants grown under water stress conditions. World Journal of Agricultural Sciences 2(1): 37-46.
• Wang, X., Li, D., & Zahang, X. (1999). Relationship between irrigation amount and yield of cucumber in Solor greenhouse. China Vegetables Journal 1: 1-6.
• Wright, H., Delong, J., Lada, R. & Prange, R. 2009. The relationship between water status and chlorophyll a fluorscence in grapes (Vitis ). Postharvest Biology and Technology 51: 193–199. https://doi.org/10.1016/j.postharvbio.2008.07.004.
• Wu, R., & Garg, A. (2003). Engineering rice plants with trehalose-producing genes improves tolerance to drought, salt, and low temperature. ISB news report 3-7.
• Xiao, X., Xu, X., & Yang, F. (2008). Adaptive responses to progressive drought stress in two Populus cathayana Silva Fennica 42(5): 705-719.
• Zhang, J., & Kirkham, M.B. (1996). Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytologist 132: 361-373. https://doi.org/10.1111/j.1469-8137.1996.tb01856.x.
• Zhang1991, J., & Davies, W.J. (1991). Antitranspirant activity in xylem sap of maize plants. Journal of Experimental Botany 42(3): 317-321. https://doi.org/10.1093/jxb/42.3.317.
• Zhao, D. Y., Shen, L., Fan, B., Liu, K.L., Yu, M.M., Zheng, Y., Ding, Y., & Sheng, J.P. (2009). Physiological and genetic properties of tomato fruits from 2 cultivars differing in chilling tolerance at cold storage. Journal of Food Science 74(5): 348-352. https://doi.org/10.1111/j.1750-3841.2009.01156.x.