Effect of Different Fertilizers and Planting Density on Morphological Traits and Photosynthetic Pigments Content in Isabgol (Plantago ovata Forsk)

پژوهشهای زراعی ایران

Volume 19, Issue 4 - Serial Number 64, January 2022, Pages 327-341   

Document Type: Research Article

DOI: 10.22067/jcesc.2021.37183.0

Authors

M Khavari¹; M. A Behdani^{* 1}; H. R Fallahi²

¹Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Birjand, Birjand, Iran

²Plant and Environmental Stresses Research Group, Faculty of Agriculture, University of Birjand, Birjand, Iran

Abstract

Introduction
Medicinal plants in the world are very important and currently the demand for medicinal herbs is on the rise due to higher requirement of products need in the pharmaceutical, health and food industry. Therefore, the assessment of various systems of plant nutrition is one of the important needs of agricultural planning in order to achieve high yield with high quality, especially in medicinal plants. Manure increase soil fertility and improves the growth and yield. This experiment was conducted to investigate the effect of planting density and single or combined application of cow manure (CM) and chemical fertilizers (CF) on growth, yield and pigment content of Isabgol (Plantago ovata Forsk).
Materials and Methods
In order to evaluate the effects of different levels of fertilizer on isabgol (Plantago ovata Forsk) an experiment was arranged as factorial based on a randomized complete block design with three replications at Sarayan Faculty of Agriculture, University of Birjand, during 2017. Experimental factors were included: Fertilization treatments consisted of single (40 ton.ha^-1 CM and 40 kg.ha^-1 CF from the source of NPK) and combined (40 ton.ha^-1 CM + 20 kg.ha^-1 CF and 20 ton. CM + 40 kg.ha^-1 CF) application of organic and chemical fertilizers along with a control treatment (no-fertilizer) which were evaluated at three levels of density (20, 40 and 60 plants.m²). Measurements of chlorophyll a, b, carotenoid and SPAD: Firstly, 0.1g samples were taken from the collected leaves. Subsequently each sample was extracted by 80% acetone and put in the centrifuges with rotation speed of 6000 per minute for 10 minute. Absorbance using a spectrophotometer at wavelengths of 470, 647, 663 nm was measured. Also, chlorophyll index was measured by manual chlorophyll meter. Measurements of Yield and yield components: In the sampling stage (full flowering stage), 3 plants of cultivation lines were harvested with respect to the elimination of marginal effects. After measuring plant height, the plants were cut from above the soil, immediately weighed (fresh weight) and then the samples were placed in separate bags and after drying in an oven at 72^°C for 48 hours, the leaf weight, total dry weight and grain yield of each sample were measured. Finally, Statistical analyses were carried out by ANOVA, with  least significant difference test (LSD) at 5% probability level for subsequent pairwise comparisons.
Results and Discussion
 The results indicated the significant effect of treatments on plant dry weight, biological yield, seed yield and chlorophyll b content. The highest biological and seed yields (2080 and 806.6 kg.ha^-1, respectively), were obtained from planting density of 60 plants m², while the highest leaf chlorophyll content was gained from 20 plants m² treatment. The effect of fertilizer resources on all traits (except plant dry weight) and interaction effect of experimental factors on all studied indices were no-significant. The plant showed an appropriate adaptation to low nutritional and its maximum yield was obtained when at least 60 plants.m² was used.
Conclusion
Based on the obtained results, it could be concluded that isabgol is a low-input medicinal plants in terms of nutrient requirements. Therefore, it seems that the application of fertilizer resources in crops which are located prior to isabgol in a rotation and availability of remaining nutrients can provide the nutritional requirements of this plant. Moreover, consider to open canopy of the plant even when the plant density was 60 plant.m^-2, it seems that more densities must be considered in future studies.

Keywords

: Carotenoids; Chlorophyll; Manure (Cow manure); SPAD

References

1. Adediran, J. A., Taiwo, L. B., Akande, M. O., Sobulo, R. A., and Idowu O. J. 2004. Application of organic and inorganic fertilizer for sustainable maize and cowpea yields in Nigeria. Journal of Plant Nutrition 27: 1163-1181.
2. Ahmadian, A., Ghanbari, A., and Galavi, M. 2006. Effect of animal manure on quantitative and qualitative yield and chemical composition of essential oil in cumin (Cuminum cyminum). Iranian Journal of Field Crops Research 4: 1-10. (In Persian with English abstract).
3. Aminpour, R., and Mousavi, S. F. 1996. Effects of irrigation frequency on development stages, yield and yield components of cumin. Agriculture and Natural Resources 1 (1): 1-7.
4. Arancon, N. Q., Edwards, C. A., Bierman, P., Welch, C., and Metzeger J. D. 2004. Influences of vermicomposts on field emergence, development and grain yield of winter wheat (Triticum aestivum) and winter barley (Hordeum vulgare L.). Seed Science and Technology 21: 159-178. (In Persian with English abstract).
5. Arnon, A. N. 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal 23: 112-121.
6. Ashraf, M. Y., Azmi, A. R., Khan, A. H., and Ala, S. A. 1994. Effect of water stress on total phenols, peroxide activity and chlorophyll content in wheat. Acta Physiologiae Plantarum 16 (3): 185-197.
7. Ashraf, M., Ali, Q., and Rha, E. S. 2006 Effect of varying nitrogen regimes on growth, seed yield, and nutrient accumulation in isabgol. Journal of Plant Nutrition 29 (3): 535-542.
8. Berimavandi, A. R., Hashemabadi, D., Ghaziani, M. V. F., and Kaviani, B. 2011. Effects of plant density and sowing date on the growth, flowering and quantity of essential oil of Calendula officinalis Journal of Medicinal Plant Research 5: 5110-5115. (in Persian).
9. Chatterjee, S. K. 2002. Cultivation of medicinal and aromatic plants in India, a commercial approach. Acta Horticulture 576: 191-202.
10. Daman, M. J. 1999. Principles of Food Chemistry (Chapter 3) Maryland Publishers.
11. Darzi, M. T., Ghalavand, A., Rajali, F., and Sefidkon, F. 2006. Investigation on biological fertilizers effects on yield and yield components of Foeniculum vulgare Iranian Journal of Medicinal and Aromatic Plants 22 (4): 292-276. (in Persian with English abstract).
12. Dorry, M. A., Hosseini, S. A., and Babakhanloo, P. 2005. Effect of planting date and seed rate on seed production of Plantago ovata in Marahtepeh. Final report of the research project. Agricultural and Natural Resources Research Center of Golestan Province. (in Persian).
13. Dorry, M. A. 2009. Correlation of yield components with quantitative and qualitative seed oil production of Plantago ovata under dry conditions of Maraveh Tappeh. Research and construction in Natural Resources 79: 173-178. (in Persian).
14. Ehteramian, K. 2003. Effect of different levels of nitrogen fertilizer and planting date on yield and yield components of cumin in Kooshkak Region of Fars Province. M. Sc. thesis on Desert Areas Management, Faculty of Agriculture, Shiraz University. (in Persian)
15. Eskandari, H., Zahtab salmasi, S., Ghasemi Golzan, K., and Ghariha, M. H. 1999. Effect of water restriction on grain yield and oil yield of sesame. Journal of Food Agriculture and the Environment 7 (2): 339-342.
16. Fallahi, H. R., Taherpour Kalantari, R., Asadian, A. H., Aghhavani-Shajari, M., and Ramazani, H. R. 2018. Effect of different soil fertilizing agents on growth and yield of isabgol and black seed as two medicinal plants. Iranian Journal of Field Crop Science. (in Persian with English abstract).
17. Ghasemi Siani, E., Fallah, S., and Tadayyon, A. 2011. Study on yield and seed quality of Plantago ovata Forssk under different nitrogen treatments and deficit irrigation. Iranian Journal of Medicinal and Aromatic Plants 27 (3): 517-528. (in Persian with English abstract).
18. Gill, M. S., and Narang, R. S. 1993. Yield analysis in Gobbi Sarson (Brassica napus) to plant density and nitrogen. Indian Journal of Agronomy 38:257-265.
19. Hamzei, J. M., Seyedi, M., and Babaei, M. 2015. Effect of density and nitrogen on seed quantity and quality of winter rapeseed in Hamedan conditions. Journal of Crop Production 8 (1): 143-159.
20. Heidari, F., Zehtab-Salmasi, S., Javanshir, A., Aliari, H., and Dadpour, M. 2008. Effect of plant density on yield and essential oil production of peppermint (Mentha piperita). Journal of Agricultural Science and Technology 45 (2): 501-510. (in Persian).
21. Javadi, H., Rashedmohassel, M. V. and Nasrabadi, A. S. 2008. Effect of plant density on agronomic traits, chlorophyll content and shoot transfer percentage in four cultivars of Sorghum bicolor. Iranian Journal of Crop Research 5 (2): 271-280. (in Persian with English abstract).
22. Jones, C., Olson-Rutz, K., and Pariera Dinkins, C. 2011. Nutrient uptake timing by crop to assist with fertilizing decisions. Available online at: http://landresources.montana.edu/soilfertility/
23. Karimzadeh, G., and Omidbaigi, R. 2004. Growth and seed characteristics of isabgol (Plantago ovata Forsk) as influenced by some environmental factors. Journal of Agricultural Science and Technology 6: 103-110.
24. Khajepour, M. R. 2005. Industrial plants. Agricultural Jihad Publishing House of Isfahan. 581 pages. (in Persian).
25. Khandan, A., Astaraeii, A., Nassiiri Mahalati, M., and Fotovvat, A. 2005. Effects of organic and inorganic fertilizers on yield and yield components of Plantago ovata Iranian Journal of Field Crop Research 3 (2): 243-253. (in Persian with English abstract).
26. Khavari, M., Behdani, M. A., and Fallahi, H. R. 2019. Influence of planting density, single and combined application of cow manure and chemical fertilizer on mucilage yields in Plantago ovata. Journal of Agroecology 11 (3): 1139-1150.
27. Koocheki, A., Bkhshaey, S., Tabraey, A., and Jasfary, B. 2015. Evaluation of the effect of plant density and culture patterns on quantitative and qualitative characteristics of Lallemantia royleana Benth. Journal of Agroecology 6 (2): 229-237. (in Persian with English abstract).
28. Latmahalleh, D. A., Niyaki, S. A. N., and Vishekaei, M. N. S. 2011. Effects of plant density and planting pattern on yield and yield components of Echium amoenum in North of Iran. Journal of Medicinal Plant Research 5: 932-937.
29. Lotfi, A., Vahabi Sedehi, A., Ghanbari, A., and Heydari, M. 2009. The effect of deficit irrigation and manure on quantity and quality traits of plantago ovata in Sistan region. Iranian Journal of Medicinal and Aromatic Plants 24 (4): 506-518. (in Persian with English abstract).
30. Mandal, K., Saravanan, R., and Maiti, S. 2008. Effect of different levels of N, P and K on downy mildew (Peronospora plantaginis) and seed yield of isabgol (Plantago ovata). Crop Protection 27 (6): 988-995.
31. Maurya, R. P., Bailey, J. A., Jeff, S. T., and Chandler, A. 2013. Impact of plant spacing and picking interval on the growth, fruit quality and yield of okra (Abelmoschus esculentus). American Journal of Agriculture and Forestry 1 (4): 48-54.
32. McNeil, DL. 1991. Growth of Plantago ovata in north Western Australia in response to sowing date; sowing rate and mothod of sowing. Tropical Agriculture 63 (3): 289-295.
33. Moradi, R., Rezvani Moghaddam, P., Nassiri Mahallati, M., and Lakzian, A. 2009. Effects of biological and organic fertilizers on yield, yield components and essential oil of Foeniculum vulgare. Iranian Journal of Field Crops Research 7 (2): 625-635. (in Persian with English abstract).
34. Moraditochaee, M., Motamed, M. K., Azarpour, E., and Khosravi Danesh, R. 2012. Effects of nitrogen fertilizer and plant density management in corn farming. Journal of Agriculture and Biological Science 7: 133-137.
35. Mosavi, S.G.R., Segatoleslami, M. J., and Pooyan, M. 2012. Effect of planting date and plant density on yield and seed yield components of Plantago ovata Iranian Journal of Medicinal and Aromatic Plants 27 (4): 681-699. (in Persian with English abstract).
36. Najafi, F., and Rezvani Moghaddam, P. 2001. Effect of irrigation intervals and plant density on the quality and quantity of medicinal plant of Plantago ovata. M.Sc Thesis of Agriculture, Faculty of Agriculture, Ferdowsi University of Mashhad. (in Persian).
37. Najafi, F., and Rezvani Moghaddam, P. 2002. Effect of different irrigation and density regimes on yield and crop characteristics of Plantago ovata Journal of Agricultural Sciences and Technology 16 (1): 59-65.
38. Omrani, B. 2015. The response of production and shelf-life of purslane plant to nitrogen and phosphorus supply from different fertilizer sources. M.Sc. thesis of Agroecology. Faculty of Agriculture. Shahrekord University. P: 141. (in Persian).
39. Pouryousef, M., Mazaheri, D., Chaiechi, M. R., Rahimi, A., and Tavakoli, A. 2011. Effect of different soil fertilizing treatments on some of agro morphological traits and mucilage of Isabgol (Plantago ovata Forsk.). Electronic Journal of Crop Production 3 (2): 193-213. (in Persian with English abstract).
40. Rahimi, A., Jahansoz, M. R., and Rahimian Mashhadi, H. 2014. Effect of drought stress and plant density on quantity and quality charactristics of isabgol (Plantago ovata) and French psyllium. Journal of Crop Production and Processing 4 (12): 143-156. (in Persian with English abstract).
41. Rahmati, M., Azizi, M., Hassanzadeh, M., and Neamati, H. 2009. The effect of different levels of plant density and nitrogen on morphological traits, yield, essential oil content and percentage of chamomile (Matricaria recutita). Journal of Horticulture Science and Technology 23 (1): 27-35. (in Persian with English abstract).
42. Ramash, M. N., Farooqi, A. A., and Subbaiah, T. 1984. Influence of sowing date and nutrient on growth and yield of Isabgul (Plantago ovata). Crop Research 2 (2): 169-174.
43. Rao, P. S., Mishra, B., Gupta, S. R., and Rathore, A. 2008. Reproductive stage tolerance to salinity and alkality stresses in rice genotypes. Plant Breeding 127: 256-261.
44. Russelle, M. P., Wilhelm, W. W., Olson, R. A., and Power, J. F. 1984. Growth analysis based on degree days. Crop Science 24: 28- 32. (in Persian with English abstract).
45. Sabagh Nekonam, M., and Razmjoo, K. H. 2007. Effect of plant density on yield, yield components and effective medicine ingredients of blond psyllium (Plantago ovata Forsk) Accession. International Journal of Agriculture and Biology 9 (4): 606-609. (in Persian with English abstract).
46. Shah, Z., and Ahmad, M. I. 2006. Effect of integrated use of farm yard manure and urea on yield and nitrogen uptake of wheat. Journal of Agricultural and Biological Science 1 (1): 60-65.
47. Sharifi Ashoorabadi, A. 1998. Survey of soil fertility in ecosystems. Ph.D Thesis in Agriculture, Islamic Azad University, Science and Research Branch. (in Persian).
48. Singer, J. W., Kohler, K. A., Liebman, M., Richard, T. L., Cambardella, C. A., and Buhler, D. D. 2004. Tillage and compost effect on yield of corn, soybean, and wheat and fertility. Agronomy Journal 96: 531-537.
49. Soltaninezhad, F. 2013. Effect of solitary and integrated application of urea fertilizer and cattle manure on cadmium concentration and yield of purslane (Portulaca oleraceae) medicinal plant. M.Sc. Thesis of Agroecology. Faculty of Agriculture. Shahrekord University. 107p.
50. Tabrizi, L. 2004. Effects of water stress and manure on quantitative and qualitative criteria of Plantago ovata and Plantago psyllium. MSc Thesis, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. (in Persian with English abstract).
51. Vaishya, R. D., and Fayaz Qazi, M. 1992. Chlorophyll content in chickpea as influenced by seed rate and weed management practices. Chickpea Newsletters 26: 26-27.
52. Varvel, G. E., Schepersand, J. S., and Francis, D. D. 1997. Ability for in season correction of nitrogen deficiency in corn using chlorophyll meters. Soil Sciences Society of America Journal 61: 1233-1239.
53. Yadave, R. D., Keswa, G. L., and Yadva, S. S. 2002. Effect of integrated use of FYM, and urea and sulphur on growth and yield of isabgol (Plantago ovata). Journal of Medicinal and Aromatic Plant science 25: 668-671.
54. Zahoor, A., Ghafor, A., and Muhammad, A. 2004. Plantago ovata- A crop of arid and dry climates with immense herbal and pharmaceutical importance. Introduction of Medicinal Herbs and Spices as Crops Ministry of Food. Agriculture and Livestock, Pakistan.
55. Zotarelli, L., Scholberg, J. M., Dukes, M. D., and Muoz Carpena, R. 2008. Fertilizer residence time affects nitrogen uptake efficiency and growth of sweet corn. Journal of Environmental Quality 37: 1271-1278.