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Shahzeidi, Z., Hesami Tackallou, S., Amjad, L., Zali, H., Iranbakhsh, A. (2022). The Effect of Ultraviolet (UV) Radiation on Photosynthetic Pigments and Biochemical Parameters of Portulaca oleracea. , 36(1), 319-328. doi: 10.22067/jhs.2021.71685.1077
Z. Shahzeidi; S. Hesami Tackallou; L. Amjad; H. Zali; A. Iranbakhsh. "The Effect of Ultraviolet (UV) Radiation on Photosynthetic Pigments and Biochemical Parameters of Portulaca oleracea". , 36, 1, 2022, 319-328. doi: 10.22067/jhs.2021.71685.1077
Shahzeidi, Z., Hesami Tackallou, S., Amjad, L., Zali, H., Iranbakhsh, A. (2022). 'The Effect of Ultraviolet (UV) Radiation on Photosynthetic Pigments and Biochemical Parameters of Portulaca oleracea', , 36(1), pp. 319-328. doi: 10.22067/jhs.2021.71685.1077
Shahzeidi, Z., Hesami Tackallou, S., Amjad, L., Zali, H., Iranbakhsh, A. The Effect of Ultraviolet (UV) Radiation on Photosynthetic Pigments and Biochemical Parameters of Portulaca oleracea. , 2022; 36(1): 319-328. doi: 10.22067/jhs.2021.71685.1077

The Effect of Ultraviolet (UV) Radiation on Photosynthetic Pigments and Biochemical Parameters of Portulaca oleracea

علوم باغبانی
Article 22, Volume 36, Issue 1 - Serial Number 53, March 2022, Pages 319-328    PDF (894.89 K)
Document Type: Research Article
DOI: 10.22067/jhs.2021.71685.1077
Authors
Z. Shahzeidi1; S. Hesami Tackallou* 1; L. Amjad2; H. Zali3; A. Iranbakhsh1
1Biology, Faculty of Basic Sciences, Islamic Azad University, Sciences and Research Branch, Tehran, Iran
2Biology, IbnSina Faculty, Islamic Azad University Falaverjan Branch, Isfahan, Iran
3Faculty of Medical Tissue Engineering, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Introduction
 UV-C (254-280 nm) and 280-320 nm) UV-B, UV-A (320-390nm) wavelengths are irradiated with three ultraviolet strips and have detrimental effects on the growth of a number of plants. Ultraviolet light is an important non-living factor that can stimulate the production of secondary metabolites, including antioxidant compounds in plants. Ozone depletion and its consequences, including direct UV radiation on the planet and its effects on crops and medicinal plants, are among the topics that have received very little study. Ultraviolet light in nature occurs only at low intensities, but if the inhibitory effect of the ozone layer in the stratosphere is significantly the result of nitrogen and hydrocarbon oxides the weaker the halogen, the higher its amount.
Materials and Methods
 Portulaca oleracea seeds were prepared by Pakan Isfahan Company. The aim of this study was the effect of ultraviolet rays at different levels (UV-C: 0, 100, 200, 300, 400, 500, 600, and 700 nm) on the activity of photosynthetic pigments and biochemical traits of portulaca oleracea in factorial in a completely randomized design with three replications. After transferring the seeds of portulaca oleracea, the healthy and uniform seeds of this plant were sterilized in 15% sodium hypochlorite solution for 154 minutes and then washed thoroughly with distilled water and placed in a petri dish for germination. Moisture was supplied through filter paper soaked in distilled water. The seeds were planted in pots filled with cocopeat and perlite evenly and watered for 20 days with a half-strength Hoagland solution. Plants were grown for 20 days at a temperature of 30 ± 2 ° C and a light period of 8.16 (light / dark, respectively). Plants for one week, every other day, and for 3 minutes each time by two fluorescent lamps with a wavelength of 260 nm exposed to ultraviolet C (at a distance of 30 cm from the UV light source with an intensity of 27 (w / m2) were located. The traits studied in this study included chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, phenol, flavonoids, and antioxidant activity. In this study, the effect of ultraviolet light on the activity of photosynthetic pigments and biochemical traits of portulaca oleracea was investigated factorially in a completely randomized design with three replications.
Results and Discussion
 The results of the mean comparison showed that the UV treatment of chlorophyll a, b, total chlorophyll, carotenoid of portulaca oleracea was reduced compared to the control; However, UV treatment of portulaca oleracea significantly increased phenol, flavonoids, and antioxidants compared to the control. The effect of different doses of ultraviolet rays on phenol and portulaca oleracea antioxidants showed that the UV-C highest and lowest were 700 and 100 nm, respectively. Decreases in carotenoid content can result in either inhibition of pigment synthesis or their breakdown and degradation. The results of this report indicate significant changes in phenols and flavonoids as compounds it absorbed ultraviolet rays compared to control cells.
Conclusion
 It can be said that excessive exposure to radiation may affect chlorophyll levels by inhibiting chlorophyll biosynthesis or accelerating its degradation. Oxygen is an electron receptor in the electron transport system that produces energy from adenosine triphosphate (ATP) in the body. Under certain conditions, oxygen can be converted to a single electron, creating free radicals. When oxygen is converted to a single electron, it is called active oxygen (ROS). These free radicals cause oxidative stress in plants which oxidative stress leads to damage to macromolecules such as DNA, proteins and so on. Environmental stresses, including UV radiation, produce active oxygen species (ROS) such as hydrogen peroxide (H2O2), superoxide (O2-), and hydroxyl radicals (OH), which cause oxidative stress and cause damage to cells, such as DNA. And cause the destruction of these compounds. The plant contains compounds that act as active antioxidants and sweep away active oxygen. In the present study, the observed increase in phenols, flavonoids and antioxidants indicates an increase in the production of free radicals under ultraviolet radiation and shows that the production of these radicals is more than the plant's defense capacity and has caused damage to plant biological membranes. In summary, the application of controlled ultraviolet light stress can provide a new alternative strategy to increase the productivity of the portulaca oleracea plant. Modulating UV-C light in agricultural systems is a promising tool to increase crop production.
 
Keywords
Antioxidant; Extract; Flavonoid; Phenol
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