Optimization of Seed Germination, Growth Index and Photosynthetic Pigments Content of Kelussia odoratissima Mozaff Seedlings under Laboratory Conditions

علوم باغبانی

Article 12, Volume 36, Issue 3 - Serial Number 55, November 2022, Pages 693-707   

Document Type: Research Article

DOI: 10.22067/jhs.2021.73715.1111

Authors

Kh. Ahmadi¹; H. Omidi^{* 1}; M. Amini¹; E. Soltani²

¹Department of Agronomy and Plant Breeding, College of Agriculture and Medicinal Plants Research Center, Shahed University, Tehran, Iran

²Department of Agronomy and Plant Breeding Sciences, College of AbuRaihan, University of Tehran, Tehran, Iran

Abstract

Introduction
 Kelussia odoratissima Mozaff is a native species of Iran which is a rare and endangered species. It grows as a wild in cold and mountainous bioclimatic and is used in traditional medicine to treat various diseases such as cardiovascular disease, gastric ulcer, respiratory and intestinal inflammation. The change of status from dormancy to germination can be eliminated by using some treatments in accordance with the natural conditions of the mother base habitat. However, some physiological needs of dormant seeds can be met by scratching (mechanical and chemical), washing in running water, dry storage, cold and humid conditions, light, smoke, and plant growth regulators. The aim of this study was to investigate different strategies including pretreatment, leaching and constant germination temperature on seed germination characteristics and Kelussia seedling growth.
Materials and Methods
 The experiment was conducted in Petri dishes at Seed Technology Laboratory of Agricultural Sciences Faculty of Shahed University. K. odoratissima Mozaff seeds were collected from their natural habitat in Fereydounshahr, Isfahan province in 2019.
This study was performed in the Crop Physiology and Seed Technology Laboratories of Shahed University, Faculty of Agricultural Sciences, from 23.09.2019 to 22.11.2019. The cultivation was in Petridish at constant germination temperatures after priming and leaching. The experiment was performed as a factorial experiment in a completely randomized design with three replications. Each replication included 36 Petridish and 20 Kelussia seeds were planted in each petri dish. Experimental factors include constant germination temperatures (1, 5, 10 and 15°C), duration of rinsing with running water at 15°C (24, 48 and 72 h) and hormone pretreatment with gibberellin (0, 250 and 500 ppm). Before applying the hormonal pretreatment and temperature, the seeds were washed in running water in such a way that seeds were placed in a strainer that was not immersed and water flowed on the seeds for the specified periods of time for this treatment. In this case, germination inhibitors were washed from the seed surface. According to the test period and laboratory conditions, the laboratory temperature could be controlled at 15 °C with a thermometer and cooling devices. Then, for hormonal pretreatment, the seeds were placed in containers containing gibberellin solution with concentrations of 0, 250 and 500 ppm and refrigerated at 4°C for 72 h. After washing the seeds, 20 seeds were placed in Petridish with a diameter of 10 cm and a height of 2 cm on Whatman filter paper No. 1 and at temperatures of 1, 5, 10 and 15°C with 16 h of light and 8 h of darkness passed. Due to the fact that germination in seeds grown at this temperature at 15°C was zero in all treatment compositions, it was excluded from statistical analysis. To analyze the data variance, the SAS 9.1 statistical software was used. The comparison of means of traits was performed using the Duncan test at 5% probability level.
Results and Discussion
 Germination traits, growth indices and physiological parameters of seedling photosynthetic pigments under the influence of leaching, temperature, gibberellin and the interactions of leaching in temperature, leaching in gibberellin, temperature in gibberellin and the combination of leaching treatment × temperature × gibberellin showed significant differences. The results showed that the optimum germination temperature was 1°C and about 54% of seeds were able to germinate at this temperature without using any pretreatment. However, pretreatment of seeds at a temperature of 1°C with gibberellin at 250 ppm and washing for 72 h increased the germination rate to 65%. It has also been shown that treatment with gibberellin at 250 ppm seedling length and gibberellin at 500 ppm improves seedling fresh and dry weight in three leaching treatments at 10°C. Chlorophyll and carotenoid content of seedlings was observed in the combination of 24 hours leaching treatment, temperature of 5°C and gibberellin priming of 500 ppm. Due to the wide variety of species of Apiaceae and also the variety of type and depth of sleep, various treatments to break dormancy and stimulate seed germination of plants of this genus have been proposed, the most important of which are wet and gibberellin. It should be noted that the germination ecology and appropriate treatments to break dormancy in different plant species, plants of the same family, same species and different ecotypes of the same species can be completely different.
Conclusion
 According to the results of this study, seed treatment with 72 hours of cold water washing, 1°C and gibberellin pretreatment with a concentration of 250 ppm was able to show the highest germination percentage to achieve High germination is recommended. In addition, at 5°C under gibberellin pretreatment and leaching showed a relatively high germination percentage. Accordingly, gibberellin hormonal pretreatment at low temperatures was effective in achieving more germination under priming conditions. Is. On the other hand, a concentration of 500 ppm gibberellin increased seedling weight and photosynthetic pigments. In general, a temperature of 1°C followed by a temperature of 5°C was effective in increasing the germination of celery seeds and was able to record better results. Also, the suitable seedling growth temperature for mountain celery is 10°C and the application of Gibberellin hormonal pretreatment improved the growth characteristics of Kelussia seedlings.

Keywords

Chlorophyll; Germination temperature; Gibberellin; Kelussia; Leaching

References

• Abbaspour, H., & Rezaei, H. (2015). Effects of gibberellic acid on Hill reaction, photosynthetic pigment and phenolic compounds in Moldavian dragonhead (Dracocephalum moldavica ) in different drought stress levels.  Journal of Plant Research (Iranian Journal of Biology) 27(5): 893-903. (In Persian with English abstract)
• Afzaligroh, S., Mahdinezhad, N., Azadghojehbiglo, H., & Salarnia, N. (2018). The effect of chilling and leaching in removing dormancy the seeds of Lovage (Levisticum officinale KOCH). Journal of Seed Research 8(1): 60-68. https://doi.org/20.1001.1.22520961.1397.8.26.6.8.
• Ahmadi, K., Omidi, H., Amini, Dehaghi, M., & Naghdi Badi, H.A. (2020). Review on the botanical, phytochemical and pharmacological characteristics of Kelussia odoratissima Journal of Medicinal Plants 18(72): 30-45. https://doi.org/10.29252/jmp.4.72.S12.30.
• Ahmadi, K., Omidi, H., Amini Dehaghi, M., & Soltani, E. (2021). Evaluation of dormancy breaking treatments on seed germination and soluble compounds of Kelussia odoratissima seedling. Plant Physiology Reports 26(3): 513-525. https://doi.org/10.1016/S0076-6879(84)05016-3.
• Chaparzadeh, N., Rahimpourshafaii, L., Dolati, M., & Barzegar, A. (2013). Age dependent changes of pigments in Rosa hybrid. Journal of Plant Research (Iranian Journal of Biology) 26(3): 281-289. (In Persian with English abstract)
• Costache, M.A., Campeanu, G. & Neata, G. (2012). Studies concerning the extraction of chlorophyll and total carotenoids from vegetables. Romanian Biotechnological Letters 17(5): 7702-7708.
• Durr, C., Dickie, J.B., Yang, X.Y., & Pritchard, H.W. (2015). Ranges of critical temperature and water potential values for the germination of species worldwide: contribution to a seed trait database. Agricultural and Forest Meteorology 200: 222-232.
• EL-Tayeb, M.A. (2005). Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation 3: 215-225. https://doi.org/10.1007/s10725-005-4928-1.
• Etemadi, N., Haghighi, M., Nikbakht, A., & Zamani, Z. (2010). Methods to promote germination of Kelussia odoratissima, an Iranian endemic medicinal plant. Herba Polonica 56: 21-28.
• Farooq, M., Basra, S.M.A., Ahmad, N., & Hafeez, K. (2005). Thermal hardening: a new seed vigor enhancement tool in rice. Journal of Integrative Plant Biology 47(2): 187-193. https://doi.org/1111/j.1744-7909.2005.00031.x.
• Finch Savage, W.E., & Leubner Metzger, G. (2006). Seed dormancy and the control of germination. New Phytology 171: 501–523.
• Galindez, G., Seal, C.E., Daws, M.I., Lindow, L., Ortega‐Baes, P., & Pritchard, H.W. (2017). Alternating temperature combined with darkness resets base temperature for germination (Tb) in photoblastic seeds of Lippia and Aloysia (Verbenaceae). Plant Biology 19(1): 41-45. https://doi.org/doi/abs/10.1111/plb.12449.
• Gandomkar, M. (1999). Phytochemical study of cereal escape oil. Ph.D., Faculty of Pharmacy, Tehran University of Medical Sciences. Tehran. Iran. (In Persian)
• Ganji Arjenaki, F., Amini Dehaghi, M., & Jabbari, R. (2011). Effects of priming on seed germination of marigold (Calendula officinalis). Advances in Environmental Biology 5: 276–280.
• Golmohamdzadeh, S., Zaefarian, F., & Rezvani, M. (2015). Effects of some chemical factors, prechilling treatments and interactions on the seed dormancy breaking of two Papaver species. Weed Biology and Management 15(1): 11-19. https://doi.org/doi/10.1111/wbm.12056.
• Gonzalez-Benito, M., Albert, E., Iriondo, M.J., Varela, J.M., & Perez-Garca, F. (2004). Seed germination of four thyme species after conservation at low temperatures at several moisture contents. pp. 247-254.
• Gupta, V. (2003). Seed germination and dormancy breaking techniques for indigenous medicinal and aromatic plants. Journal of Medicinal and Aromatic Plants Science 25: 402–407.
• International Seed Testing Association (ISTA), (2010). International rules for seed testing, Bassersdorf, Switzerland.
• Jamshidi-Kia, F., Lorigooini, Z., & Hossein Amini-Khoei, (2018). Medicinal plants: Past history and future perspective. Journal Herbmed Pharmacology 7(1): 1-7. https://doi.org/10.15171/jhp.2018.01.
• Karim, M.N., Sani, M.N.H., Uddain, J., Azad, M.O.K., Kabir, M.S., Rahman, M.S., Choi, KY., & Naznin, M.T. (2020). Stimulatory effect of seed priming as pretreatment factors on germination and yield performance of yard long bean (Vigna unguiculata). Horticulturae 6(4): 104.
• Mozaffarian, V. (2007). Umbelliferae: Flora of Iran fundamentals of analytical chemistry. Grupo Editorial Norma. Iran. 1072p. (In Persian)
• Nawaz, A., Amjad, M., Khan, S.M., Afzal, I., Ahmed, T., Iqbal, Q., & Iqbal, J. (2013). Tomato seed invigoration with cytokinins. Journal of Animal and Plant Sciences 23(1): 121-128.
• Nowruzian, A., Masoumian, M., Ebrahimi, M.A., & Bakhshi khaniki, G.R. (2017). Effect of breaking dormancy treatments on germination of Ferula assa-foetida Iranian Journal of Seed Research 3(2): 155-169. (In Persian with English abstract). https://doi.org/10.29252/yujs.3.2.155.
• Omidi, H., Naghdi Badi, H.A., & Jafarzadeh, L. (2015). Seeds of medicinal plants and crops. Shahed University. pp: 454. (In Persian)
• Paucar-Menacho, L.M., Martinez-Villaluenga, C., Duenas, M., Frias, J., & Penas, E. (2016). Optimization of germination time and temperature to maximize the content of bioactive compounds and the antioxidant activity of purple corn (Zea mays ) by response surface methodology. LWT-Food Science and Technology 76: 236-244.
• Payam Noor, V., & Kordalivand, A. (2018). The effect of different seed dormancy breaking treatments on germination and primary functions of Betula pendula. Plant Research 29: 309–318.
• Pirmoradi, M., Omidbaigi, R., Naghavi, M., Baghizadeh, A., & Yadollahi, A. (2013). Effect of elevation and different treatments on Asafetida (Ferula assa-foetida) seed germination. Iranian Journal of Horticultural Science 43(4): 461-471. (In Persian with English abstract). https://doi.org/10.22059/ijhs.2012.29380.
• Rostami, G., Moghaddam, M., Narimani, R., & Mehdizadeh, L. (2018). The effect of different priming treatments on germination, morphophysiological, and biochemical indices and salt tolerance of basil (Ocimum basilicum cv. Keshkeni Levelou). Environmental Stresses in Crop Sciences 11(4): 1107-1123. https://doi.org/10.22077/ESCS.2018.1072.1213.
• Saberi, M., & Karimian, V. (2018). Influence of chemical stimulants on improvement of growth, support and retrofit components of the medicinal plant Datura Stramonium under stress with allelopathic compounds Eucalyptus camaldulensis. Pasture 12: 401–410.
• Shaddad, M.A.K., Abd El- Samad, M., & Mostafa, D. (2013). Role of gibberellic acid (GA₃) in improving salt stress tolerance of two wheat cultivars. International Journal of Plant Physiology and Biochemistry 5(4): 50-57. https://doi.org/20.1001.1.24763780.1395.3.3.1.9.
• Sharifi, H. Nemati, A., & Gerdakaneh, M. (2017). Breaking seed dormancy and improve germination of four medicinal species of apiaceae under gibberellic acid and prechilling treatments. Iranian Journal of Seed Science and Research 4(3): 27-38. (In Persian with English abstract). https://doi.org/22124/JMS.2017.2505.
• Sharifi, H., Khajeh Hosseini, M., & Rashed Mohassel, M.H. (2015). Study of seed dormancy in seven medicinal species from Apiaceae. Iranian Journal Seed Research 2: 25-36. (In Persian with English abstract). https://doi.org/10.29252/yujs.2.1.25.
• Slabbert, M.M., Motsa, M. & Van Averbeke, W. (2014). Germination of selected African leafy vegetables in response to different dormancy pre-sowing treatments. In: XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014). 1102: 75-82. https://doi.org/17660/ActaHortic.2015.1102.8.
• Soltani, E., Mortazavian, S.M.M., Faghihi, S., & Akbari, G.A. (2019). Non-deep simple morphophysiological dormancy in seeds of Cuminum cyminum Journal of Applied Research on Medicinal and Aromatic Plants 15: 100222. https://doi.org/10.1016/j.jarmap.2019.100222.
• Vojodi Mehrabani, L., & Valizadeh Kamran, R. (2020). The effects of seed priming on some germination index, enzymatic activity and some physiological traits of Capparis spinose and Kelussia odoratissima. Iranian Journal of Seed Science and Research 7(2): 229-240. (In Persian with English abstract). https://doi.org/22124/JMS.2020.4574.
• Yousefi, F., Siahpoush, A.R, Bakhshandeh, A.A., & Mousavi, S.A. (2021). The effect of hormone seed priming using gibberellic acid on seed germination characteristics and seedling growth of coneflower (Echinacea purpurea). Iranian Journal of Seed Research 8(1): 173-188. (In Persian with English abstract). https://doi.org/52547/yujs.8.1.173.
• Zandalinas, S.I., Mittler, R., Balfagón, D., Arbona, V., & Gomez‐Cadenas, A. (2017). Plant adaptations to the combination of drought and high temperatures. Physiologia Plantarum 162(1): 2-12. https://doi.org/1111/ppl.12540.