The Effect of Foliar Application of Salicylic Acid, Spermidine and Sodium Nitroprusside on some Growth and Flowering Characteristics, Photosynthetic Pigments and Vase Life of Lisianthus ‘Mariachi Blue’

علوم باغبانی

Article 14, Volume 36, Issue 4 - Serial Number 56, January 2023, Pages 917-936   

Document Type: Research Article

DOI: 10.22067/jhs.2022.74334.1118

Authors

M. Piri; Z. Jabbarzadeh^*

Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran

Abstract

Introduction
 Lisianthus (Eustoma grandiflorum) from Gentianaceae family is from wild flowers of north and west America. Lisianthus, a relatively new floral crop to the international market, quickly ranked in the top ten cut flowers worldwide due to its rose-like and blue flowers. It is also widely used as a flowering potted and bedding plant. Lisianthus ‘Mariachi Blue’ is cultivated as a cut flower. Salicylates have very beneficial effects on plant growth and development. The effect of phenolic compounds in many biochemical and physiological processes including photosynthesis, ion adsorption, membrane permeability, enzyme activity, flowering, stimulation of plant resistance systems, heat production and plant development has been proven. The most famous member of this group is salicylic acid, which as a simple phenolic compound, is naturally produced by plants. Salicylic acid (SA) is considered to be plant signal molecule that plays a key role in plant growth, development, and defense responses. Polyamines (PAs) are ubiquitous and biogenic amines that have been implicated in cellular functions in living organisms. In plants they have been implicated in a wide range of biological processes including cell division, cell elongation, senescence, embryogenesis, root formation, floral initiation and development, fruit development and ripening, pollen tube growth and plant responses to biotic and abiotic stress. Sodium nitroprusside is a nitric oxide releasing agent. Nitric oxide is a gaseous free radical that can disperse very rapidly through cell membranes due to its gaseous nature and medium shelf life, without a carrier. Nitric oxide (NO) is an unstable environmentally-friendly gas radical that is used to protect the postharvest longevity of different horticultural crops. In addition to controlling harvested crop senescence, NO is involved in many plant processes, e.g., germination, growth and development, photosynthesis, pigment synthesis, defensive system, and many others. In the present study, we investigated the effects of foliar application of salicylic acid, spermidine and sodium nitroprusside on some morpho-physiological characteristics and vase life of lisianthus flowers ‘Mariachi Blue’.
Materials and Methods
 This study was conducted based on a completely randomized design with 10 treatments, 4 replications which each replication containing 2 pots. The treatments were included spermidine at concentrations of 0.5, 1 and 2 mM, salicylic acid at concentrations of 0.5, 1 and 1.5 mM, sodium nitroprusside at concentrations of 50, 100 and 200 μM and control (without any application of growth regulators) as foliar application at intervals of 15 days for 2 months. Plant characteristics including leaf area, stem length, fresh and dry weight of leaves and flower, number of buds, flowers’ length and diameter, photosynthetic pigments and vase life were assayed. To perform analysis of variance and compare the mean of the studied traits, SAS software version 9.1 was used. The means were compared using the Tukey multi-domain method at a probability level of 1%. Also, Excel (2016) software was used to draw the chart.
 
Results and Discussion
 The results obtained from analysis of variance in this study showed that the effect of growth regulators used in the study was significant at the level of 1% probability on all morphological traits measured, photosynthetic pigments and vase life of lisianthus flowers. Mean comparison graphs showed that salicylic acid, spermidine and sodium nitroprusside had a positive effect on some morphological traits, photosynthetic pigments and vase life compared to control. It can be concluded that, salicylic acid caused to increase all parameters except the flowers’ fresh weight compared to control. Spermidine increase stem length, leaf fresh weight, flowers’ fresh and dry weight, length, and diameter, chlorophyll index, chlorophyll b, and carotenoid and vase life of flowers. Also, sodium nitroprusside had beneficial effects on all parameters in this research except leaf area, leaf dry weight, chlorophyll a and vase life of flowers. Salicylic acid plays an important role in regulating some physiological processes of plants such as growth and development, ion uptake and transport, stomatal conductivity, and membrane permeability, which is effective in plant photosynthesis and with increasing photosynthesis, plant growth rate increases. Polyamines such as spermidine are involved in a wide range of developmental stages including cell division, embryogenesis, root growth, and flowering. Sodium nitroprusside is involved in the most important plant processes such as photosynthesis, respiration, growth and cell division. Probably, these growth regulators, due to their effect on plant growth, flowering, as well as photosynthetic pigments, have caused the increment of plant biomass and vase life.
Conclusion
 In the present study, the effect of salicylic acid, spermidine and sodium nitroprusside on some growth and flowering characteristics, photosynthetic pigment and vase life of Eustoma grandiflorum ‘Mariachi Blue’ was assayed. According to the results of the present study, it can be concluded that these growth regulators improved growth indices, flowering parameters, photosynthetic pigment and vase life of flowers. According to the results, the appropriate concentrations for salicylic acid were 1 mM, for spermidine, 1 mM and for sodium nitroprusside were also 50 and 100 μM.
 

Keywords

Chlorophyll content; Flower diameter; Growth regulator; Nitric oxide; Polyamine

References

1. Abbasi, N.A., Ali, I., Hafiz, I.A., & Khan, A.S. (2017). Application of polyamines in horticulture: A review. International Journal of Biosciences 10(5): 319-342. https://doi.org/10.12692/ijb/10.5.319-342.
2. Afshari, M., Shekari, F., Azimkhani, R., Habibi, H., & Fotokian, M.H. (2013). Effects of foliar application of salicylic acid on growth and physiological attributes of cowpea under water stress conditions. Iran Agricultural Research 32(1): 55-70.
3. Aelaei, M., Mirzaei Mashoud, M., & Mortazavi, S.N. (2017). Effect of postharvest salicylic acid treatment on physico-chemical attributes and vase-life of rose (Rosa hybrida Hater Class) cut flowers. Plant Production Technology 17(1): 47-33. (In Persian with English abstract)
4. Alcazar, R., & Tiburcio, A.F. (2018). Polyamines. Springer. 509 p.
5. Amorim, T.L., Medeiros, D.C., Oliveira, A.S., Paes, A.R., Júnior, W.S., & Moreira, D.A. (2017). Gibberellin and polyamines in plant growth, development and postharvest senescence of ornamental plants – a review. Amazonian Journal of Plant 1: 1-13. https://doi.org/10.26545/B00000X.
6. Arun, M., Naing, A.H., Jeon, S.M., Ai, T.N., Aye, T., & Kim, C.K. (2017). Sodium nitroprusside stimulates growth and shoot regeneration in chrysanthemum. Horticulture, Environment and Biotechnology 58(1): 78-84. https://doi.org/10.1007/s13580-017-0070-z.
7. Asghari, M.R. (2015). Classic plant growth hormones and regulators. University of Urmia, 352 p. (In Persian)
8. Asna Ashari, M., & Zokai Khosroshahi, M. (2008). Polyamines and horticultural sciences. Bu Ali Sina University Press. 163 p. (In Persian)
9. Azooz, M.M., & Youssef, M.M. (2010). Evaluation of heat shock and salicylic acid treatments as inducers of drought stress tolerance in Hassawi wheat. American Journal of Plant Physiology 5(2): 56-70. https://doi.org/10.3923/ajpp.2010.56.70.
10. Chen, D., Shao, Q., Yin, L., Younis, A., & Zheng, B. (2019). Polyamine function in plants: metabolism, regulation on development, and roles in abiotic stress responses. Frontiers in Plant Science 9: 1945. https://doi.org/10.3389/fpls.2018.01945.
11. Cho, M.S., Celikel, F.G., Dodge, L., & Reid, M.S. (2001). Sucrose enhances the postharvest quality of cut flower of Eustoma grandiflorum (Raf.) shinn. Acta Horticulturae 543: 304-315. https://doi.org/10.17660/ActaHortic.2001.543.37.
12. Dastyaran, M., & Hosseini Farahi, M. (2014). Effect of humic acid and putrescine on growth and flowering characteristics of roses in soilless system. Journal of Science and Technology of Greenhouse Cultures 20: 243-252. (In Persian)
13. Deng, Y., Wang, C., Huo, J., Hu, W., & Liao, W. (2019). The involvement of NO in ABA-delayed the senescence of cut roses by maintaining water content and antioxidant enzymes activity. Scientia Horticulturae 247: 35-41. https://doi.org/10.1016/j.scienta.2018.12.006.
14. Edrisi, B. (2006). Lisianthus. Publications of the Research Institute of Flowers and Ornamental Plants. 51 p. (In Persian)
15. Ershad Langroudi, M., Hashemabadi, D., Kalate Jari, S., & Asadpour, L. (2020). Effects of pre- and postharvest applications of acid salicylic on the vase life of cut Alstroemeria flowers (Alstroemeria hybrida). Journal of Horticulture and Postharvest Research 3(1): 115-124. https://doi.org/10.22077/jhpr.2019.2409.1053.
16. Eslami, M., Nasibi, F., Manouchehri Kalantari, K., Khezri, M., & Oloumi, H. (2019). Effect of exogenous application of L-arginine and sodium nitroprusside on fruit abscission and physiological disorders of pistachio (Pistacia vera) Scions. International Journal of Horticultural Science and Technology 6(1): 51-62. https://doi.org/10.22059/ijhst.2019.270762.265.
17. Fan, H.F., Du, C.X., Ding, L., & Xu, Y.L. (2014). Exogenous nitric oxide promotes waterlogging tolerance as related to the activities of antioxidant enzymes in cucumber seedlings. Russian Journal of Plant Physiology 61(3): 366-373. https://doi.org/10.1134/S1021443714030042.
18. Gad, M.M.., Abdul-Hafeez, E.Y., & Ibrahim, O.H.M. (2016). Foliar application of salicylic acid and gibberellic acid enhances growth and flowering of Ixora coccinea plants. International Journal of Plant Production 7(1): 85-91. https://doi.org/10.21608/jpp.2016.43477.
19. Ghilavizadeh, A., Hadidi Masouleh, E., Zakerin, H.R., Valadabadi, S.A.R., Sayfzadeh, S., & Yousefi, M. (2019). Influence of salicylic acid on growth, yield and macro-elements absorption of Fennel (Foeniculum vulgare ) under water stress. Journal of Medicinal Plants and By-products 1: 67-75.
20. Gupta, D.K., Palma, J.M., & Corpas, F.J. (2019). Nitric oxide and hydrogen peroxide signaling in higher plants. Springer, 275p.
21. Hadi, H., Najafabadi, A., & Amirnia, R. (2014). Comparison of different treatment methods of salicylic acid on some physiological traits of white bean under salinity stress. Cercetari Agronomics in Moldova 47(3): 97-105. https://doi.org/10.2478/cerce-2014-0030.
22. Hohn, D., Peil, R.M.N., Marchi, P.M., Grolli, P.R., Perin, L., & Rosa, D.S.B. (2019). Growth and quality of lisianthus [Eustoma grandiflorum (Shinn.)] cultivated in rice husk substrates in troughs with leaching recirculation. Revista Colombiana De Ciencias Hortícolas 13(3): 458-465. https://doi.org/10.17584/rcch.2019vl3i3.9891.
23. Hosseini Farahi, M., & Aboutalebi Jahroomi, A. (2018). Effect of pre-harvest foliar application of polyamines and calcium sulfate on vegetative characteristics and mineral nutrient uptake in Rosa hybrida. Journal of Ornamental Plants 8(4): 241-253.
24. Hosseini, H., & Rezainejad, A. (2016). The effect of foliar application of sodium nitroprusside on drought stress tolerance in marigold. Iranian Journal of Horticultural Science and Technology 17(3): 298-285. (In Persian with English abstract)
25. Jabbarzadeh, M., Tehraniifar, A., Amiri, J., & Abedi, B. (2015). Investigation of the protective role of nitric oxide in reducing damage caused by salinity stress in marigold (Calendula officinalis cv. Gitan Orange). Agricultural Sciences and Industries 30(2): 191-185. (In Persian with English abstract)
26. Jabbar, A., Tehranifar, A., Shour, M., & Nemati, H. (2018). Effect of putrescine and different media on vegetative growth, floret and some biochemical parameters of gladiolus under soilless conditions. International Journal of Current Microbiology and Applied Sciences 7: 71-80.
27. Jalili Marandi, R., Hassani, A., Abdollahi, A., & Hanafi, S. (2011). Improvement of the vase life of cut gladiolus flowers by essential oils, salicylic acid and silver thiosulfate. Journal of Medicinal Plants Research 5(20): 5039-5043.
28. Janda, T., Szalai, G., & Pál, P. (2020). Salicylic acid signaling in plants. International Journal of Molecular Sciences 21(2655): 1-6. https://doi.org/10.3390/ijms21072655.
29. Kafi, M., Daneshvar, N., Nikbakht, A., Hakimi, M., Rajali, F., & Daneshkhah, M. (2013). Effect of humic acid and mycorrhizal fungus on some characteristics of Lolium combination of white speed green. Journal of Science and Technology of Greenhouse Cultures 4(13): 49-58. (In Persian with English abstract)
30. Kahrobaiyan, M., Nemati, S.H., Rahemi, M., Kholdebarin, B., & Teranifar, A. (2019). Morphological responses of ornamental sunflower to putrescine treatment under drought conditions. Applied Ecology and Environmental Research 17(3): 6117-6127. https://doi.org/10.15666/aeer/1703_61176127.
31. Khan, N.A., Syeed, S., Masood, A., Nazar, R., & Iqbal, N. (2010). Application of salicylic acid increases contents of nutrients and antioxidative metabolism in mungbean and alleviates adverse effects of salinity stress. International Journal of Plant Biology 1: 1-8. https://doi.org/10.4081/pb.2010.e1.
32. Khazaei, Z., & Estaji, A. (2019). Effects of foliar application of salicylic acid on some of morphological parameters pepper seedlings under drought stress conditions. 2nd international & 6th national confrence on organic and conventional agriculture, Ardabil, https://civilica.com/doc/932166.
33. Khosh-khoui, M., Sheibani, B., Rouhani, E., & Tafazzoli, A.A. (2010). Principles of horticulture. Shiraz University Press, 19^th Edition, 596 p. (In Persian)
34. Kusano, T., & Suzuki, H. (2015). Polyamines. Springer, 336p.
35. Lichtenthaler, H.K., & Buschmann, C. (2001). Extraction of photosynthetic tissues: chlorophylls and carotenoids. Current Protocols in Food Analytical Chemistry2.1-F4.2.6. https://doi.org/10.1002/0471142913.faf0402s01.
36. Loutfy, N., El-Tayeb, M.A., Hassanen, A.M., Moustafa, M.F., Sakuma, Y., & Inouhe, M. (2012). Changes in the water status and osmotic solute contents in response to drought and salicylic acid treatments in four different cultivars of wheat (Triticum aestivum). Journal of Plant Research 125(1): 173-184. https://doi.org/10.1007/s10265-011-0419-9.
37. Miri, S.M., Savari, A., Behzad, K., & Mohajer Iravani, B. (2016). Promotion of callus initiation, shoot regeneration and proliferation in Lisianthus. Iranian Journal of Plant Physiology 6(4): 1855-1860. https://doi.org/10.30495/ijpp.2016.532656.
38. Mohammad Saeed, A.A.J., Abdulhadi, M.D., & Salih, S.M. (2019). Response of gerbera (Gerbera jamesonii) cv. 'Great Smoky Mountains' to foliar application of putrescine, spermidine and salicylic acid. International Conference on Agricultural Sciences 388: 1-11. https://doi.org/10.1088/1755-1315/388/1/012067.
39. Nabigol, A., Ghodsi, Sh., & Hadavi, E. (2016). The effect of putrescine and salicylic acid on growth and flowering characteristics of Dahlia. 1^st International and 2nd National Ornamental Plants Congress, 23-25 August, Ferdowsi University, Mashhad, Iran, Pp. 1-5.
40. Nahar, K., Hasanuzzaman, M., Alam, M.M., Rahman, A., Suzuki, T., & Fujita, M. (2016). Polyamine and nitric oxide crosstalk: antagonistic effects on cadmium toxicity in mung bean plants through upregulating the metal detoxification, antioxidant defense and methylglyoxal detoxification systems. Ecotoxicology and Environmental Safety 126: 245-255. https://doi.org/10.1016/j.ecoenv.2015.12.026.
41. Najm, A.A., Haj Seyed Hadi, M.R., Fazeli, F., Darzi, M.T., & Rahi, A. (2012). Effect of integrated management of nitrogen fertilizer and Cattle manure on the leaf chlorophyll, yield and tuber glycoalkaloids of Agria potato. Communications in Soil Science and Plant Analysis 43: 912-923. https://doi.org/10.1080/00103624.2012.653027.
42. Poor, P., Gémes, K., Horváth, F., Szepesi, A., Simon, M.L., & Tari, I. (2011). Salicylic acid treatment via the rooting medium interferes with stomatal response, CO₂ fixation rate and carbohydrate metabolism in tomato, and decreases harmful effects of subsequent salt stress. Plant Biology 13(1): 105-114. https://doi.org/10.1111/j.1438-8677.2010.00344.x.
43. Salachna, P., & Zawadzińska, A. (2018). Effect of nitric oxide on growth, flowering and bulb yield of Eucomis autumnalis. In VII International Conference on Managing Quality in Chains (MQUIC2017) and II International Symposium on Ornamentals 1201: 635-640. https://doi.org/10.17660/ActaHortic.2018.1201.85.
44. Setia, N., & Setia, R.C. (2018). Polyamines: An overview and prospects in crop improvement. Punjab Agricultural University 21: 376-393.
45. Seyed Hajizadeh, H., & Aliloo, A.A. (2013). The effectiveness of pre-harvest salicylic acid application on physiological traits in lilium (Lilium longiflorum) cut flower. International Journal of Scientific Research in Environmental Sciences 1(12): 344-350. https://doi.org/10.12983/IJSRES-2013-P344-350.
46. Seyf, M., Khalighi, A., Mostofi, Y., & Naderi, R. (2012). Effect of sodium nitroprusside on vase life and postharvest quality of a cut rose cultivar (Rosa hybrida Utopia). Journal of Agricultural Science 4(12): 174-181. https://doi.org/10.5539/jas.v4n12p174.
47. Shabanian, S., Esfahani, M.N., Karamian, R., & Tran, L.S.P. (2018). Physiological and biochemical modifications by postharvest treatment with sodium nitroprusside extend vase life of cut flowers of two gerbera cultivars. Postharvest Biology and Technology 137: 1-8. https://doi.org/10.1016/j.postharvbio.2017.11.009.
48. Shahmoradi, H., & Naderi, D. (2018). Improving effects of salicylic acid on morphological, physiological and biochemical responses of salt-imposed Winter Jasmine. International Journal of Horticultural Science and Technology 5(2): 219-230. https://doi.org/10.22059/ijhst.2018.259507.246.
49. Shimizu, H., & Ichimura, K. (2010). Postharvest physiology and technology of cut eustoma flowers. Journal of Japanese Society of Horticultural Science 79(3): 227-238. https://doi.org/10.2503/jjshs1.79.227.
50. Vazirimehr, M.R., & Rigi, Kh. (2014). Effect of salicylic acid in agriculture. International Journal of Plant, Animal and Environmental Sciences 4(2): 291-295.
51. Vieira, M.R.S., Moura, F.D., Simões, N.A., Souza, A.V., Santos, C.M.G., Paes, R.A., & Leal, H.Y. (2017). Application of polyamine and boron improves quality of potted gerbera cv. Kosak. Journal of Applied Horticulture 19(1): 1-5. https://doi.org/10.37855/jah.2017.v19i01.15.
52. Wang, M., Li, B., Zhu, Y.C., Niu, L.J., Jin, X., Xu, Q.Q., & Liao, W.B. (2015). Effect of exogenous nitric oxide on vegetative and reproductive growth of oriental lily ‘Siberia’. Horticulture, Environment and Biotechnology 56(5): 677-686. https://doi.org/10.1007/s13580-015-0051-z.
53. Wu, Q.S., Zou, Y.N., Liu M., & Cheng, K. (2012). Effects of exogenous putrescine on mycorrhiza, root system architecture, and physiological traits of Glomus mosseae colonized trifoliate orange seedlings. Notulae Botanicae Horti Agrobotanici ClujNapoca 40(2): 80-85. https://doi.org/10.15835/nbha4027926.
54. Yousefi, F., Jabbarzadeh, Z., Amiri, J., & Rasouli-Sadaghiani, M.H. (2019). Response of roses (Rosa hybrida ‘Herbert Stevens’) to foliar application of polyamines on root development, flowering, photosynthetic pigments, antioxidant enzymes activity and NPK. Scientific Reports 9(16025): 1-11. https://doi.org/10.1038/s41598-019-52547-1.
55. Zarei, M., Sinaki, J.M., Rahbari, A., & Abbaspour, H. (2013). Effects of planting date and salicylic acid on physiological traits of forage maize hybrids. Iranian Journal of Plant Physiology 3(2): 687-693.
56. Zeb, A., Fazal Ullah, E., Syeda Leeda Gul, E., Maaz Khan, E., Zainub, B., Noman Khan, M., & Amin, N. (2017). Influence of salicylic acid on growth and flowering of Zinnia cultivars. Science International (Lahore) 29(6): 1329-1335.