Effect of Some Inorganic and Organic Fertilizers’ Application and Drought Stress on Superoxide Dismutase and Peroxidase Activities and Total Soluble Protein of Bidane-Ghermez Grapevines

علوم باغبانی

Article 3, Volume 37, Issue 2 - Serial Number 58, August 2023, Pages 325-336   

Document Type: Research Article

DOI: 10.22067/jhs.2023.73313.1102

Authors

A. Fatemi^{* 1}; A. Safari¹; M. Saeidi²; Z. Kolahchi³

¹Department of Soil Science, Razi University, Kermanshah, Iran

²Department of Agronomy, Razi University, Kermanshah, Iran

³Department of Soil Science, Bu-Ali Sina University, Hamadan, Iran

Abstract

Introduction
 Drought is the most important limiting factor for the yield and quality of products in the world. Fertilizer application can mitigate drought stress in plants. Most farmers prefer to use inorganic (chemical) fertilizers because of their fast impact on plant growth. The reasonable cost of inorganic fertilizers and their availability are the other reasons. However, the long-term effects of organic fertilizers on plant growth and soil fertility has been proved. The grapevines are one of the most important garden products in the world as well as in Iran. It has been reported that the application of biochar has significant effects on soil fertility as well as plant growth. Biochars have properties such as highly porous structure, high specific surface area, higher water holding capacity of the soil, the long-term stability in the soil. In addition, our previous study indicated that it could decrease the hazard of sodium in soil compared with compost. In Iran, the cultivation area of grapevines was the second grade after the pistachio cultivation area in 2019. According to statistical data on agriculture in 2019, the cultivation area of irrigated and rain-fed grapevines in Kermanshah province was 7710 and 1515 ha, respectively. According to this report, the production of irrigated and rain-fed grapevines in Kermanshah province in 2019 was 82718 and 1763 tons, respectively. The effect of application of some inorganic and organic fertilizers under drought stress on yield, water use efficiency, and some biochemical characteristics of the grapevine leaves cultivar Bidane-Ghermez has been previously studied. In this research, the effect of drought stress and the application of potassium sulfate, compost, and biochar on antioxidant activities of the grapevine leaves cultivar Bidane-Ghermez was investigated.
 
Materials and Methods
A greenhouse study with a factorial experiment based on a block of randomized completely design was conducted with fertilizer treatments including control, potassium sulfate (1250 mg kg^-1), compost (5% w/w), and biochar (10% w/w) without and with drought stress conditions (80 and 40% FC, respectively) during 2017-2018. For this research, the biochar was produced from apple tree pruning under low oxygen conditions by slow pyrolysis at 400 ^◦C. The compost was purchased from the municipal compost factory of Kermanshah province. To evaluate drought stress and inorganic and organic fertilizers' application on antioxidant enzymes activities, the superoxide dismutase and peroxidase activities and total soluble protein of grapevine leaves were determined. The data were analyzed by ANOVA and the mean of studied characteristics was compared by Duncan’s test at 5% probability level using SAS software.
Results and Discussion
the results indicated that the interaction effect of fertilizers treatments and drought stress on the superoxide dismutase and peroxidase activities as well as total soluble protein was significant (P<0.01). Under drought stress conditions, among fertilizers’ treatments, the activity of superoxide dismutase for potassium sulfate was significantly lower than compost and biochar treatments (P<0.01). The results also showed that the peroxidase activity for compost was significantly higher than potassium sulfate and biochar treatments (P<0.01). Besides, the highest total soluble protein amount was found for biochar treatment (P<0.01).
 
Conclusion
 according to the results of this study, it can be concluded that the increase of total soluble protein of grapevine leaves under drought stress conditions by biochar application contributed to the increase of superoxide dismutase and peroxidase activities also increasing the bioavailability of macro-and micronutrients. Biochar application increased significantly the potassium concentration of the leaves of the grapevines. Potassium regulates water movement at the plant and also provides a higher relative water content of grapevine leaves. Moreover, potassium decreases the non-stomatal effects are also attributed to stomatal closure during drought stress conditions. Furthermore, potassium contributes to protein synthesis. Biochar application also increased the phosphorus, copper, and magnesium of the leaves of the grapevine. Higher concentration of phosphorus under drought stress conditions caused the grapevine leaves to suffer less from phosphorus deficiency. Copper exists in superoxide dismutase and is considered a key player in superoxide detoxification. Under drought stress conditions, higher manganese concentration by biochar application resulted in more protection of the cellular membrane against Reactive Oxygen Species (ROS). For fertilizer recommendation in order to reduce drought stress effects on some antioxidant enzymes activities of Bidane-Ghermez grapevine leaves, the application of biochar is recommended first then potassium sulfate and compost. 

Keywords

Biochar; Compost; Macronutrients; Micronutrients; Potassium sulfate

References

1. Abedi, T., & Pakniyat, H. (2010). Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding, 46(1), 27-34. http://dx.doi.org/10.17221/17267/12009-CJGPB
2. Amini, Z., Hadad, R., & Moradi, F. (2009). The effect of water deficit stress on antioxidant enzymes during generative growth stages in barley (Hordeum vulgare L.). Isfahan University of Technology-Journal of Crop Production and Processing, 12(46), 65-74. (In Persian)
3. Beers, R.F., & Sizer, I.W. (1952). A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biology Chemistry, 195(1), 133-140. http://dx.doi.org/110.1016/S0021-9258(1019)50881-X
4. Biederman, L.A., & Harpole, W.S. (2013). Biochar and its effects on plant productivity and nutrient cycling: a meta‐ GCB Bioenergy, 5(2), 202-214. http://dx.doi.org/210.1111/gcbb.12037
5. Blokhina, O., Virolainen, E., & Fagerstedt, K.V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany, 91(2), 179-194. http://dx.doi.org/110.1093/aob/mcf1118
6. Candan, N., & Tarhan, L. (2003). The correlation between antioxidant enzyme activities and lipid peroxidation levels in Mentha pulegium organs grown in Ca²⁺, Mg²⁺, Cu²⁺, Zn²⁺ and Mn²⁺ stress conditions. Plant Science, 165(4), 769-776. http://dx.doi.org/710.1016/S0168-9452(1003)00269-00263
7. Castrillo, M., & Trujillo, I. (1994). Ribulose-1, 5-bisphosphate carboxylase activity and chlorophyll and protein contents in two cultivars of French bean plants under water stress and rewatering. Photosynthetica, 30, 175-181.
8. Conesa, M., De La Rosa, J., Domingo, R., Banon, S., & Pérez-Pastor, A. (2016). Changes induced by water stress on water relations, stomatal behaviour and morphology of table grapes (cv. Crimson Seedless) grown in pots. Scientia Horticulturae, 202, 9-16. http://dx.doi.org/10.1016/j.scienta.2016.1002.1002
9. Cramer, G.R., Van Sluyter, S.C., Hopper, D.W., Pascovici, D., Keighley, T., & Haynes, P.A. (2013). Proteomic analysis indicates massive changes in metabolism prior to the inhibition of growth and photosynthesis of grapevine (Vitis vinifera ) in response to water deficit. BMC Plant Biology, 13(1), 1-22. https://doi.org/10.1186/1471-2229-1113-1149
10. Davoudi, M.H., Shahbazi, K., Feizollahzadeh Ardebil, M. & Rezaie, H. (2015). Methods of organic fertilizers’ analysis. Soil and Water Research Institute, Technical publication No. 531. (In Persian).
11. Gambetta, G.A., Herrera, J.C., Dayer, S., Feng, Q., Hochberg, U., & Castellarin, S.D. (2020). The physiology of drought stress in grapevine: towards an integrative definition of drought tolerance. Journal of Experimental Botany, 71(16), 4658-4676. http://dx.doi.org/4610.1093/jxb/eraa4313
12. Haider, G., Koyro, H.-W., Azam, F., Steffens, D., Müller, C., & Kammann, C. (2015). Biochar but not humic acid product amendment affected maize yields via improving plant-soil moisture relations. Plant and Soil, 395(1), 141-157. http://dx.doi.org/110.1007/s11104-11014-12294-11103
13. Hammerschmidt, R., Nuckles, E. & Kuć, J. (1982). Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiological Plant Pathology, 20(1), 73-82. http://dx.doi.org/10.1016/0048-4059(1082)90025-X
14. Joseph, S., Graber, E., Chia, C., Munroe, P., Donne, S., Thomas, T., Nielsen, S., Marjo, C., Rutlidge, H., & Pan, G.-X. (2013). Shifting paradigms: development of high-efficiency biochar fertilizers based on nano-structures and soluble components. Carbon Management, 4(3), 323-343. http://dx.doi.org/310.4155/cmt.4113.4123
15. Khoshgoftarmanesh, A.H. (2014). Principles of Plant Nutrition, Isfahan University of Technology press.
16. Liu, J., Schulz, H., Brandl, S., Miehtke, H., Huwe, B., & Glaser, B. (2012). Short‐term effect of biochar and compost on soil fertility and water status of a Dystric Cambisol in NE Germany under field conditions. Journal of Plant Nutrition and Soil Science, 175(5), 698-707. http://dx.doi.org/610.1002/jpln.201100172
17. Malusà, E., Laurenti, E., Ghibaudi, E., & Rolle, L. (2002). Influence of organic and conventional management on yield and composition of grape cv.'Grignolino'. XXVI International Horticultural Congress: Viticulture-Living with Limitations 640.
18. Mohammadi Torkashvand, A., Tofighi Alikhani, T., Kaviani, B., & Ghasemnejad, M. (2018). Impact of potassium on the yield of lily (Lilium longifolium× Asiatic Termoli) and antioxidant enzymes activity under drought stress. Journal of Plant Process and Function, 7(25), 29-38.
19. Pan, Y., Wu, L.J. & Yu, Z.L. (2006). Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant Growth Regulation, 49(2), 157-165. http://dx.doi.org/110.1007/s10725-10006-19101-y
20. Ramzani, P.M.A., Shan, L., Anjum, S., Ronggui, H., Iqbal, M., Virk, Z.A., & Kausar, S. (2017). Improved quinoa growth, physiological response, and seed nutritional quality in three soils having different stresses by the application of acidified biochar and compost. Plant Physiology and Biochemistry, 116, 127-138. http://dx.doi.org/110.1016/j.plaphy.2017.1005.1003
21. Reddy, A.R., Chaitanya, K.V., & Vivekanandan, M. (2004). Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology, 161(11), 1189-1202. http://dx.doi.org/1110.1016/j.jplph.2004.1101.1013
22. Safari, A. (2018). Comparison of compost, biochar and potassium application on grapes properties under drought stress. M.Sc. Razi University.
23. Safari, A., Fatemi, A., Saiedi, M., & Kolahchi, Z. (2020). Effect of fertilizer management systems and water stress conditions on nutritional status of grapes (a greenhouse study). Journal of Soil Management and Sustainable Production, 10(1), 119-136 (In Persian with English abstract).
24. Safari, A., Fatemi, A., Saiedi, M., & Kolahchi, Z. (2022). Effect of drought stress on water use efficiency of grapevines (Vitis vinifera L.) cultivar ‘Bidaneh-Ghermez’ under different fertilizer treatments. Journal of Plant Research (Iranian Journal of Biology), 35(4), 836-847. (In Persian with English abstract).
25. Safari, A., Fatemi, A., Saiedi, M., & Kolahchi, Z. (2021). Some biochemical characteristic changes of grapevine ‘Bidane-Ghermez’Influenced by drought stress using organic and inorganic fertilizers. Journal of Horticultural Science, 35(2), 267-281. (In Persian with English abstract)
26. Siedi, A., Seifi, E., & Rasoli, V. (2022). Evaluation of some physiological and biochemical characteristics of several promising grapevine cultivars under low irrigation conditions. Research in Pomology, 6(2).
27. Siosemardeh, A., Ahmadi, A., Poustini, K., & Ebrahimzadeh, H. (2003). Stomatal and nonstomatal limitations to photosynthesis and their relationship with drought resistance in wheat cultivars. Iranian Journal of Agricultural Science, 34(4), 93-106. (In Persian)
28. Siosemardeh, A., Gholami, S., Bahramnejad, B., Kanouni, H., & Sadeghi, F. (2014). Effect of drought stress on compatible osmolytes content, enzyme activity and grain yield in chickpea (Cicer arietinum) genotypes. Iranian Journal of Crop Sciences, 16(2), 109-124. (In Persian)
29. Sorori, S., Asgharzade, A., Marjani, A., & Samadi, M. (2022). Evaluation of drought stress tolerance among some of grape cultivars using physiological and biochemical studies. Journal of Horticultural Science, 36(2), 373-388. (In Persian with English abstract)
30. Sparks, D.L., Page, A.L., Helmke, P.A., & Loeppert, R.H. (2020). Methods of soil analysis, part 3: Chemical methods, John Wiley & Sons.
31. Talaei, A., Ghaderi, N., Ebadi, A., & Lesani, H. (2011). Biochemical responses of grape cvs Sahani and Bidane-Sefid, subjected to progressive drought. Iranian Journal of Horticultural Science, 42(3), 301-308. (In Persian)
32. Usmani, M.M., Nawaz, F., Majeed, S., Shehzad, M.A., Ahmad, K.S., Akhtar, G., Aqib, M., & Shabbir, R.N. (2020). Sulfate-mediated drought tolerance in maize involves regulation at physiological and biochemical levels. Scientific Reports, 10(1), 1-13. http://dx.doi.org/10.1038/s41598-41020-58169-41592
33. Van den Berg, L. & Zeng, Y. (2006). Response of South African indigenous grass species to drought stress induced by polyethylene glycol (PEG) 6000. South African Journal of Botany, 72(2), 284-286. http://dx.doi.org/210.1016/j.sajb.2005.1007.1006
34. Xin, X., Zhang, J., Zhu, A., & Zhang, C. (2016). Effects of long-term (23 years) mineral fertilizer and compost application on physical properties of fluvo-aquic soil in the North China Plain. Soil and Tillage Research, 156, 166-172. http://dx.doi.org/110.1016/j.still.2015.1010.1012