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Sadeghi Nichkoohi, M., Moradi, Z., Mehrvar, M., Babaeizad, V. (2023). Molecular Detection of Three Viruses Infecting Mallow Plants in Golestan Province. , 37(4), 399-410. doi: 10.22067/jpp.2023.83549.1157
Mohadeseh Sadeghi Nichkoohi; Zohreh Moradi; Mohsen Mehrvar; Valiollah Babaeizad. "Molecular Detection of Three Viruses Infecting Mallow Plants in Golestan Province". , 37, 4, 2023, 399-410. doi: 10.22067/jpp.2023.83549.1157
Sadeghi Nichkoohi, M., Moradi, Z., Mehrvar, M., Babaeizad, V. (2023). 'Molecular Detection of Three Viruses Infecting Mallow Plants in Golestan Province', , 37(4), pp. 399-410. doi: 10.22067/jpp.2023.83549.1157
Sadeghi Nichkoohi, M., Moradi, Z., Mehrvar, M., Babaeizad, V. Molecular Detection of Three Viruses Infecting Mallow Plants in Golestan Province. , 2023; 37(4): 399-410. doi: 10.22067/jpp.2023.83549.1157

Molecular Detection of Three Viruses Infecting Mallow Plants in Golestan Province

پژوهش های حفاظت گیاهان ایران
Article 4, Volume 37, Issue 4 - Serial Number 62, October 2024, Pages 399-410    PDF (1.25 M)
Document Type: Research Article
DOI: 10.22067/jpp.2023.83549.1157
Authors
Mohadeseh Sadeghi Nichkoohi1; Zohreh Moradi* 1; Mohsen Mehrvar2; Valiollah Babaeizad1
1Department of Plant Pathology, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract
Introduction
The genus Malva (family Malvaceae) includes species of herbaceous plants which are often pluriannual and very common in borders and boundaries of cultivated fields, making them proper reservoirs for plant pathogens. They are most commonly used as ornamental plants, although they may be used as a food resource and remedy for various diseases. Common mallow (Malva sylvestris) is the most important Malva species which is used as garden flower as well as widely recognized for food and medicinal purposes. ­Malva sylvestris are reported to be affected by several viruses from different genera including malva vein clearing virus (MVCV, Potyvirus), alfalfa mosaic virus (AMV, Alfamovirus), malva-associated soymovirus 1 (MaSV1, Soymovirus) cucumber mosaic virus (CMV, Cucumovirus), and cotton leaf curl Gezira virus (CLCuGeV, Begomovirus). In this study, we attempted to identify important viruses infecting mallow plants and compare mallow isolates with other sequences in the GenBank.
Materials and Methods
In 2022-2023, twenty samples of mallow plants with viral-like symptoms on the leaves were collected from the urban landscape of Golestan province. Total RNA was extracted by DENAzist Total RNA Isolation Kit from fresh, infected mallow leaves. All samples were analyzed by RT-PCR with specific primer pairs for the detection of CMV, AMV, and MVCV. The amplified fragments in expected size of coat protein (CP) gene of each virus were visualized under UV light in agarose gel after electrophoresis, then amplified fragment of one isolate of each virus was bi-directionally sequenced. Obtained sequences were compared to data available in GenBank. The phylogenetic trees of viruses were constructed based on the nucleotide sequences of the coat protein gene using the neighbor-joining method by MEGA11.
Results and Discussion
All three viruses were detected in some symptomatic samples collected from Golestan province. The most typical symptoms in positive samples were mosaic, vein clearing, and leaf malformation. Of a total of 20 sampled plants, MVCV, CMV and AMV were detected by RT-PCR in nine, five and two samples, respectively. An amplicon of each virus was selected and sequenced in both directions. BLASTn analysis of the sequenced data confirmed that the PCR-amplified fragments belonged to MVCV, AMV, and CMV. Phylogenetic analysis based on the nucleotide sequence of CP gene of MVCV (n=21) including Iranian and GenBank isolates showed that all isolates are divided into six groups: G1 to G6. All Iranian isolates along with the isolate from the Netherlands were placed in group G5. The phylogenetic tree placed the CMV sequences (n=51) into two distinct phylogroups I and II; the obtained isolate CMV-IR-Ma clustered together with isolates from Iran, Netherlands, South Korea, Japan, China, Hungary, Australia, France and the USA into group II. According to the results of this study, AMV isolates (n=17) can be divided into two groups, I and II. Group I which includes isolates from Canada, China, Italy, Spain, Argentina, Australia and the USA, was divided into six subgroups. The obtained isolate AMV-IR-Ma was clustered in subgroup IB with a Chinese isolate (HZ) and forms a common branch with isolates (Ir-VM, Ir-WS, and Ir-WS2) from Iran. This is the first report of mallow (Malva sylvestris) infection with CMV and AMV in Iran using RT-PCR. In addition, mixed infection with two (MVCV+CMV and MVCV+AMV) or all three viruses (MVCV+CMV+AMV) was also confirmed in some samples. The phylogenetic trees showed that most of the viral isolates were not grouped according to their geographic locations. This suggests the dissemination and spread of these viruses through infected seeds. In Iran, M. sylvestris is a common weed found in fields, waste grounds, roadside verges and gardens. Considering the potential non-persistent aphid transmission as well as mechanical transmission, virus-infected mallows can act as a natural reservoir, thereby posing a threat to other ornamental plants and crops. Since the studied viruses were not detected in the other symptomatic plants, the observed symptoms can be caused by physiological disorders such as nutrient deficiencies, the presence of different viral strains, or other unknown and undetected viral species.
Conclusions
This study provides new knowledge on the diversity and molecular characteristics of viruses in mallow plants (M. sylvestris) affected by the viral disease. The information obtained from this study can be helpful in improving management strategies for disease caused by these viruses in Iran. Albeit M. sylvestris is a host of these viruses, but more comprehensive research on other viral species that may infect this plant need to be conducted. Weed management could be an effective way to eliminate inoculum sources of these viruses.
 






 



 
Keywords
Viral diseases; Mallow; Sequencing; Detection
References
  1. Al‐Rubaye, A.F., Kaizal, A.F., & Hameed, I.H. (2017). Phytochemical screening of methanolic leaves extract of Malva sylvestris. International Journal of Pharmacognosy and Phytochemical Research, 9(4), 537–552. https://doi.org/10.25258/phyto.v9i4.8127
  2. Balasubramaniam, M., Ibrahim, A., Kim, B.S., & Loesch-Fries, L.S. (2006). Arabidopsis thaliana is an asymptomatic host of Alfalfa mosaic virus. Virus Research, 121(2), 215–219. https://doi.org/10.1016/j.virusres.2006.04.005
  3. Bananej, K., Shahid, M.S. & Shafiq, M. (2021). Evidence that leaf curl disease of Malva sylvestris in Iran is associated with cotton leaf curl Gezira virus and associated betasatellite. Journal of Plant Pathology, 103(2), 671–672. https://doi.org/10.1007/s42161-021-00758-9
  4. Bergua, M., Luis-Arteaga, M., & Escriu, F. (2014). Genetic diversity, reassortment, and recombination in alfalfa mosaic virus population in Spain. Phytopathology, 104(11), 1241-1250. doi: https://doi.org/10.1094/PHYTO-11-13-0309-R
  5. Bujarski, J., Figlerowicz, M., Gallitelli, D., Roossinck, M.J., & Scott, S.W. (2012). Family Bromoviridae. In: King, A.M.Q., Adams, M.J., Carstens, E.B., Lefkowitz, E.J. (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Academic Press, New York, pp 965–976.
  6. Bol, J.F. (1999). Alfalfa mosaic virus and ilarviruses: involvement of coat protein in multiple steps of the replication cycle. Journal of General Virology, 80 (Pt 5), 1089-1102. http://dx.doi.org/10.1099/0022-1317-80-5-1089
  7. Dabiri, S., Moradi, Z., Mehrvar, M. & Zakiaghl, M. (2020). Analysis of the complete genome sequence of cucumber mosaic virus from Vinca minor and Wisteria sinensis in Iran. Journal of Plant Pathology, 102, 1263–1268. https://doi.org/10.1007/s42161-020-00650-y
  8. Duarte, L.M.L., Rivas, E.B., Alexandre, M.A.V., Harakava, R. & Veauvy, M.C.D. (2013). Genealogy of Cucumber mosaic virus isolated from ornamental species. American Journal of Plant Sciences, 4, 1081-1087. https://doi.org/10.4236/ajps.2013.45134
  9. Gasparetto, J.C., Martins, C.A., Hayashi, S.S., Otuky, M.F., & Pontarolo, R. (2012). Ethnobotanical and scientific aspects of Malva sylvestris : A millennial herbal medicine. The Journal of Pharmacy and Pharmacology, 64(2), 172–189. https://doi.org/10.1111/j.2042-7158.2011.01383.x
  10. Edwardson, J.R., & Christie, R.G. (1997). Alfamovirus Genus. Alfalfa mosaic virus species. Pages 63-94 in: viruses infecting peppers and other solanaceous crops. Monogr. 18-I. University of Florida Press, Gainesville.
  11. Gao, S.Q., Liang, J., Guo, K., Zhou, X., & Su, X. (2020). First report of alfalfa mosaic virus in Cayratia japonica, Justicia procumbens and Veronica persica in China. Journal of Plant Pathology, 102(1), 267. https://doi.org/10.1007/s42161-019-00408-1
  12. García-Arenal, F., & Palukaitis, P. (2008). Cucumber mosaic virus. In: García-Arenal F, Palukaitis P, Mahy BWJ, Van Regenmortel MHV, editors. Encyclopedia of Virology. 3. Oxford: Academic Press; pp. 614–619.
  13. Heydarirad, G., Rezaeizadeh, H., Choopani, R., Mosavat, S. H., & Ameri, A. (2017). Efficacy of a traditional Persian medicine preparation for radiation‐induced xerostomia: A randomized, open‐label, active‐controlled trial. Journal of Integrative Medicine, 15, 201–208. https://doi.org/10.1016/S2095-4964(17)60333-9
  14. He, B., Fajolu, L., Wen, R-H., & Hajimorad, M.R. (2010). Seed transmissibility of Alfalfa mosaic virus in soybean. Plant Health Progress, 11, 41. https://doi.org/10.1094/PHP-2010-1227-01-BR
  15. Hiruki, C., & Hampton, R.O. (1990). Diseases caused by viruses and viruses infectious to alfalfa. Pages 51-58 in: Compendium of alfalfa diseases. D. L. Stuteville and D. C. Erwin, eds. American Phytopathological Society, St. Paul, MN.
  16. Horváth, J., Mamula, D., Besada, W.H., & Juretic, N. (1979). Some properties of Malva Vein Clearing Virus isolated in Hungary and Yugoslavia. Journal of Phytopathology, 95, 51–58. https://doi.org/10.1111/j.1439-0434.1979.tb01577.x
  17. Hu, Y., Shi, H.W., Jing, C.C., Li, K., Sun, X.C., Wu, G.T., Zhou, C.Y., & Qing, L. (2016). First report of cucumber mosaic virus infecting apple in China. Journal of Plant Pathology, 98, 181–181. https://doi.org/4454/JPP.V98I1.065
  18. Hull, R. (2014). Plant virology (5th ed.). New York: Academic Press.1098 p.
  19. Jacquemond, M. (2012). Cucumber mosaic virus, pp. 439-504. In: Loebenstein G. and Lecoq H. (Eds.). Advances in Virus Research. Academic Press. https://doi.org/10.1016/B978-0-12-394314-9.00013-0
  20. Jasper, E.M.J., & Bos, L. (1980). Alfalfa mosaic virus. CMI/AAB Descriptions of Plant Viruses No. 229. Association of Applied Biologists, Warwick
  21. Kadare´, G., & Haenni, A-L. (1997). Virus-encoded RNA heli Journal of Virology, 71, 2583–2590. https://doi.org/10.1128/jvi.71.4.2583-2590.1997
  22. King, A.M., Lefkowitz, E., Adams, M.J., & Carstens, E.B. (2012). Virus Taxonomy, Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Amsterdam. pp 1272.
  23. Lunello, P., Tourino, A., Nunez, Y., Ponz, F., & Sanchez, F. (2009). Genomic heterogeneity and host recovery of isolates of malva vein clearing virus. Virus Research, 140, 91-97. https://doi.org/10.1016/j.virusres.2008.11.006
  24. Maachi, A., Hernando, Y., Aranda, M.A., & Donaire, L. (2022). Complete genome sequence of malva-associated soymovirus 1: a novel virus infecting common mallow. Virus Genes, 58(4), 372-375. https://doi.org/10.1007/s11262-022-01900-0
  25. Massumi, H., Maddahian, M., Heydarnejad, J., Hosseini Pour, A., & Farahmand, A. (2012). Incidence of viruses infecting alfalfa in the southeast and central regions of Iran. Journal of Agricultural Science and Technology, 14 (5), 1141-1148.
  26. Moradi, Z., & Mehrvar, M. (2021). Whole-genome characterization of alfalfa mosaic virus obtained from metagenomic analysis of Vinca minor and Wisteria sinensis in Iran: with Implications for the genetic structure of the virus. Plant Pathology Journal, 37(6), 619-631. https://doi.org/10.5423/PPJ.OA.10.2021.0151
  27. Moradi, Z., & Mehrvar, M. (2023). Metagenomic analysis of Malva sylvestris from Iran displays a malva vein clearing virus genome. Journal of Agricultural Science and Technology, 25(1), 213–223. https://doi.org/52547/jast.25.1.213
  28. Ohshima, K., Matsumoto, K., Yasaka, R., Nishiyama, M., Soejima, K., Korkmaz, S., Simon, Ho SY, Gibbs, A., & Takeshita, M. (2016). Temporal analysis of reassortment and molecular evolution of cucumber mosaic virus: Extra clues from its segmented genome. Virology, 487, 188–197. https://doi.org/10.1016/j.virol.2015.09.024
  29. O’Reilly, E.K., & Kao, C.C. (1998). Analysis of RNA dependent RNA polymerase structure and function as guided by known polymerase structures and computer predictions of secondary structure. Virology, 252(2), 287–303. https://doi.org/10.1006/viro.1998.9463
  30. Ozdag, Y., & G. Sertkaya. (2017). Investigation on viruses causing yellowing disease in pepper in Hatay-Turkey. Journal of Agricultural Faculty of Mustafa Kemal University, 22(1), 16-22.
  31. Palukaitis, P., & García-Arenal, F. (2003). Cucumoviruses. Advances in Virus Research, 62, 241–323. https://doi.org/10.1016/S0065-3527(03)62005-1
  32. Parrella, G., Troiano, E., Faure, C., Marais, A., & Candresse, T. (2021). First report of alfalfa mosaic virus in chayote in Italy. Plant Disease, 105(3), 698. https://doi.org/10.1094/PDIS-10-20-2117-PDN
  33. Salamon, P., Sos-Hegedus, A., Gyula, P., & Szittya, G. (2018). First report of the infection of alfalfa mosaic virus in Salvia sclarea in Hungary. Journal of Plant Pathology, 100(3), 607. https://doi.org/10.1007/s42161-018-0117-8
  34. Smit, C.H., & Jaspars, E.M. (1982). Evidence that RNA4 of alfalfa mosaic virus does not replicate autonomously. Virology, 117(1), 271-274. https://doi.org/10.1016/0042-6822(82)90528-1
  35. Soards, A.J., Murphy, A.M., Palukaitis, P., & Carr, J.P. (2002) Virulence and differential local and systemic spread of cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Molecular Plant-Microbe Interactions, 15(7), 647–653. https://doi.org/10.1094/MPMI.2002.15.7.647
  36. Van Dun, C.M.P., Bol, J.F., & Van Vloten-Doting, L. (1987). Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants. Virology, 159(2), 299–305. https://doi.org/10.1016/0042-6822(87)90467-3
  37. Xu, H., & Nie, J. (2006). Identification, characterization, and molecular detection of Alfalfa mosaic virus in potato. Phytopathology, 96(11), 1237-1242. http://dx.doi.org/10.1094/PHYTO-96-1237
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