Effect of Different Concentrations of Salicylic Acid in Increasing Resistance to Heat Stress in Cucumber (Cucumis sativa var. Rashid)

علوم باغبانی

Article 11, Volume 38, Issue 1 - Serial Number 61, March 2024, Pages 165-177   

Document Type: Research Article

DOI: 10.22067/jhs.2023.82087.1263

Authors

L. Cheheltanan¹; S. Khosravi^{* 2}; S.H. Nemati³

¹Department of Horticultural Science and Landscape, Ferdowsi University of Mashhad, Mashhad, Iran

²Ferdowsi University of Mashhad

³Ferdowsi university of Mashhad

Abstract

Introduction
Grasses are narrow-leaved plants that are used as cover plants in landscape. These plants are one of the basic and necessary components of the green cover of most gardens, parks and as the background color of landscape. In Iran, due to the high costs of planting and management of grass, high water requirements, climatic incompatibility and damage to water and soil salinity, it is recommended to remove from the green space in some cities, especially in areas with low water and water and soil saline. If it is possible to benefit from the role and influence of these plants by observing the technical points and choosing the best species for each area. Salinity stress is the second limiting factor for the growth of plants in the world after drought, which affects the efficiency and performance of plants. Increase in salinity causes a decrease in the water potential in the soil. In this condition, the plant spends most of its energy to maintain the water potential, cell mass, and water absorption to have minimal growth. The aim of this research is the effect of external application of glycine betaine on the accumulation of osmolality compounds and the antioxidant system of sports grass under salt stress.
 
Materials and Methods
This research was done in factorial form in completely randomized design with 3 replications on Rashid variety cucumber. The factors included 4 levels of temperature (25, 30, 35 and 40 degrees Celsius) and 3 levels of salicylic acid (0, 0.5 and 1 mM). When the seedlings reached the two-leaf stage, they were sprayed with different concentrations of salicylic acid two times with an interval of five days. One week after the application of salicylic acid, temperature treatment was gradually applied. After applying each heat treatment, the corresponding pots were transferred to the greenhouse with a temperature of 25 degrees Celsius. Then, fresh and dry weight of root and shoot, leaf surface, chlorophyll index, electrolyte leakage, proline, polyphenol oxidase enzyme activity and superoxide dismutase enzyme activity were measured.
 
Results and Discussion  
The results showed that salinity stress decreased all the study morphological, physiological and biochemical parameters including plant height, shoot fresh and dry weight, number of tiller, leaf area, chlorophyll content, protein and total antioxidant capacity in the studied plants. It also increased peroxidase enzyme, H₂O₂ and proline in plants, but glycine betaine application significantly improved the morpho-physiological characteristics of plants compared to the control under salt stress conditions. Thus, the highest height, shoot fresh and dry weight, leaf area, number of tiller, chlorophyll content, and protein and antioxidant capacity were observed in plants sprayed with glycine betaine. Also, the highest content of glycine betaine and activity of catalase and peroxidase enzymes and the lowest content of glycine betaine and H₂O₂ were observed in in plants sprayed with glycine betaine and 10 mM glycine betaine was more effective than 5 mM. The occurrence of salinity in plants disrupts the absorption of ions and causes the reduction of nutrients and increases sodium ions. One of the effects of salinity in plants is the reduction of photosynthetic activity, which results in the reduction of chlorophyll, carbon dioxide absorption, photosynthetic capacity, plant height, shoot fresh and dry weight, number of tiller and leaf area. One of the most strategies to deal with stress is accumulation of osmolyte and increasing the antioxidant activity, which makes plants resistant to environmental stresses. Salinity, through the toxic effect of Na⁺ and Cl^- ions, affects the growth and performance of the plant by reducing the soil water potential, disrupting water absorption and imbalance of nutrients in the plant. The results obtained from comparing the average results of glycine betaine show that glycine betaine increased plant height, shoot fresh and dry weight, number of tiller, leaf area, chlorophyll content, total protein and antioxidant capacity, but on the other hand, it increased proline and H₂O₂ decreased, which is due to the accumulation of glycine betaine as a protector in plants under salt stress conditions. In stress conditions, glycine betaine can protect photosynthetic activities including photosynthetic enzymes, proteins and lipids in thylakoid membranes in the combination of photosystem II, and also the task of protecting cell membranes against osmotic stresses in the plant.
 
Conclusion
The results obtained from this research showed that salinity stress reduced all the morphological, physiological and biochemical characteristics in the sport grass plants, but glycine betaine application played a positive role in reducing salinity damage and maintaining plant quality. Glycine betaine is known as one of the effective molecules in stress signaling, so it can protect the plant cells against stress by reducing the destruction of the membrane and by increasing the salt tolerance mechanisms. Also, glycine betaine 10 mM is introduced as the best treatment to reduce salinity damage in sport grass during present study.

Keywords

Cucumber; Electrolyte leakage; Heat Stress; Proline; Superoxide dismutase

References

1. Amirinejad, A.A., Sayyari, M., Ghanbari, F., & kordi, S. (2017). Salycilic acid improves salinity alkalinity tolerance in pepper (Capsicum annuum). Advances in Horticultural Science, 31, 157-163. https://doi.org/10.13128/ahs-21954
2. Baninasab, B., & Ghobadi, C. (2011). Influence of Paclobutrazol and application methods on high-temperature stress injury in cucumber seedlings. Journal of Plant Growth Regulation, 30(2), 213-219. https://doi.org/10.1007/s00344-010-9188-2
3. Bates, L., Waldren, R., & Teare, I. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205-207. https://doi.org/10.1007/BF00018060
4. Bayat, H., Mardani, H., Arouie, H., & Salahvarzi, Y. (2011). Effects of salicylic acid on morphological and physiological characteristics of cucumber seedling (Cucumis sativus Super Dominus) under drought stress. Journal of Plant Production, 18, 63-76. (In Persian with English abstract). httpd://doi.org/20.1001.1.23222050.1390.18.3.5.8
5. Bingwei, Y., Shuangshuang, Y., Huoyan, Z., Riyue, D., Jianjun, L., Changming, C., & Bihao, C. (2018). Overexpression of CsCaM3 improves high temperature tolerance in cucumber. Frontiers in Plant Science, 9, 797. https://doi.org/10.3389/fpls.2018.00797
6. Chen, S., Zhao, C.B., Ren, R.M., & Jiang, J.H. (2023). Salicylic acid had the potential to enhance tolerance in horticultural crops against abiotic stress. Frontiers in Plant Science, 16, 443-457. https://doi.org/10.3389/fpls.2023.1141918
7. Ding, X., Jiang, Y., Hao, T., Jin, H., Zhang, H., He, L., Zhou, Q., Huang, D., Hui, D., & Yu, J. (2016). Effects of heat shock on photosynthetic properties, antioxidant enzyme activity, and downy mildew of cucumber (Cucumis sativus). PLoS One, 11, 4 e0152429. https://doi.org/10.1371/journal.pone.0152429
8. Giannopolitis, C.N., & Ries, S.K. (1997). Superoxid dismutase. I. occurrence in higher plants. Plant Physiology, 59, 309-314. https://doi.org/10.1104/pp.59.2.309
9. Hasanuzzaman, M.K., Nahar, M., Alam, R., Roychowdhury, & Fujita, M. (2013). Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plant. International Journal of Molecular Sciences, 14(5), 9643-9684. https://doi.org/10.3390/ijms14059643
10. Hongal, D., Raju, D., Kumar, S., Talukdar, A., Das, A., Kumari, K., Dash, P.K., Behera, T.K., Munshi, A.D., & Dey, S.S. )2023(. Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber. Frontiers in Plant Science, 20, 498-512. https://doi.org/10.3389/fpls.2023.1128928
11. Jahan, M.S., Wang, Y., Shu, Sh., Zhong, M., Chen, Z., Wu, J., Sun, J., & Guo, Sh. (2019). Exogenous salicylic acid increases the heat tolerance in Tomato (Solanum lycopersicum) by enhancing photosynthesis efficiency and improving antioxidant defense system through scavenging of reactive oxygen species. Scientia Horticulturae, 247, 421-429. https://doi.org/10.1016/j.scienta.2018.12.047
12. Karlidag, H., Yildirim, E., & Turan, M. (2009). Salicylic acid ameliorates the adverse effect of salt stress on strawberry. Scientia Agricola, 66, 180-187. https://doi.org/10.1590/S0103-90162009000200006
13. Khan, W., Prithiviraj, B., & Smith, D.L. (2003). Photosynthetic response of corn and soybean to foliar application of salicylates. Journal of Plant Physiology, 160, 485-492. https://doi.org/10.1078/0176-1617-00865
14. Kusvuran, S., & Yilmaz, U.D. (2023). Ameliorative role of salicylic acid in the growth, nutrient content, and antioxidative responses of salt-stressed lettuce. Acta Scientiarum Polonorum Hortorum Cultus, 22, 75-85.
15. Lutts, S., Kinet, J.M., & Bouharmon, J. (1996). NaCl-induced senescence in leave of rice (Oryza sativa) cultivars differing in salinity resistance. Annals of Botany, 78, 389-398. https://doi.org/10.1006/anbo.1996.0134
16. Orabi, S.A., Salman, S.R., & Shalaby, M.A. (2010). Increasing resistance to oxidative damage in cucumber (Cucumis sativus) plants by exogenous application of salicylic acid and paclobutrazol. World Journal of Agricultural Sciences, 25, 252-259.
17. Prasad, T.K. (1997). Role of catalase in inducing chilling tolerance in pre-emergance maize seedlings. Plant Physiology, 114, 1369-1376. https://doi.org/10.1006/anbo.1996.0134
18. Preet, T., Ghai, N., Jindal, S.K., & SANGHA, M. (2023). Salicylic acid and 24-Epibrassinolide induced thermotolerance in bell pepper through enhanced antioxidant enzyme system and heat shock proteins. Journal of Agricultural Science and Technology, 25, 171-183. https://doi.org/10.52547/jast.25.1.171
19. Rivero, R.M., Ruiz, J.M., Garcıa, P.C., Lopez-Lefebre, L.R., Sánchez, E., & Romero, L. (2001). Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. Plant Science, 160, 315-321. https://doi.org/10.1016/S0168-9452(00)00395-2
20. Samadi, S., Habibi, G., & Vaziri, A. (2019). Effects of exogenous salicylic acid on antioxidative responses, phenolic metabolism and photochemical activity of strawberry under salt stress. Iranian Journal of Plant Physiology, 9(2), 2685-2694. https://doi.org/10.30495/ijpp.2019.545950
21. Shakirova, F.M., Shakhabutdinova, A.R., Bezrukova, M.V., Fatkhutdinova, R.A., & Fatkhutdinova, D.R. (2003). Changes in the hormonal status of wheat seeding induced by salicylic acid and salinity. Plant Sciences, 164, 317-322. https://doi.org/10.1016/S0168-9452(02)00415-6
22. Shi, Q., Bao, Z., Zhu, Z., Ying, Q., & Qian, Q. (2006). Effects of different treatments of salicylic acid on heat tolerance, chlorophyll flurescence and antioxidant enzyme activity in seedlings of Cucumis sativa Plant Growth Regulation, 48, 127-135. https://doi.org/10.1007/s10725-005-5482-6
23. Smirnoff, N., Conklin, P.L., & Loewus, F.A. (2001). Biosynthesis of ascorbic acid in plants: a renaissance. Annual Review of Plant Physiology and Plant Molecular Biology, 52, 437-467. https://doi.org/10.1146/annurev.arplant.52.1.437
24. Soliva, R.C., Elez, P., Sebastián, M., & Martín, O. (2001). Evaluation of browning effect on avocado purée preserved by combined methods. Innovative Food Science & Emerging Technologies, 1, 261-268. https://doi.org/10.1016/S1466-8564(00)00033-3
25. Sousa, V.F.O., Santos, A.S., Sales, W.S., Silva, A.J., Gomes, F.A.L., Dias, T.J., Gonçalves-Neto, A.C., Faraz, A., Santos, J.P.O., Santos, G.L. & Cruz, J.M.F.L. (2022). Exogenous application of salicylic acid induces salinity tolerance in eggplant seedlings. Brazilian Journal of Biology 24: 84-105 https://doi.org/10.1590/1519-6984.257739
26. Taher, I.E., & Ami, S.N. (2022). Inducing systemic acquired resistance (SAR) against root-knot nematode Meloidogyne javanica and evaluation of biochemical changes in cucumber root. Helminthologia 59 (4): 404-413. https://doi.org/10.2478/helm-2022-0042
27. Taheri, M. & Haghighi, M. (2018). Benzyl adenine is more effective than potassium silicate on decreasing the detrimental effects of heat stress in pepper (Capsicum annum PS301). Iran Agricultural Research 37: 89-98. https://doi.org/10.22099/iar.2018.4890
28. Yildirim, E., Turan, M. & Guvenc, I. (2008). Effect of Foliar Salicylic Acid Applications on Growth, Chlorophyll, and Mineral Content of Cucumber Grown Under Salt Stress, Journal of plant nutrition 31 (3): 593-612. http://dx.doi.org/10.1080/01904160801895118
29. Yordanova, R. & Popova, L. (2007). Effect of exogenous treat with salicylic acid on photosynthetic activity and antioxidant capacity of chilled wheat plants. Plant physiology 33: 155-170. http://obzor.bio21.bas.bg/ipp/gapbfiles/v-33/07_3-4_155-170