Recombinant Expression of Bornavirus P24 Protein for Enzyme-Linked Immunosorbent Assay Development

Iranian Journal of Veterinary Science and Technology

دوره 16، شماره 1 - شماره پیاپی 34، خرداد 2024، صفحه 27-32   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2024.84563.1304

نویسندگان

Seyedeh Narjes Sadat¹؛ Sahar Khalvand¹؛ Behzad Ramezani²؛ Mahdi Habibi-Anbouhi³؛ Fatemeh Kazemi-Lomedasht¹؛ Hajarsadat Ghaderi¹؛ Mahdi Behdani^{* 4}

¹Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran.

²Kawsar Biotechnology Company, Tehran, Iran.

³National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.

⁴Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran & Zoonoses Research Center, Pasteur Institute of Iran, Amol, Iran.

چکیده

Borna disease virus (BDV) is a neurotropic, enveloped and ribonucleic acid (RNA) virus. BDV induces persistent neurologic disease in a wide host range included several vertebrate species as well as human. The BDV genome encodes 6 proteins but p24 protein was identified at higher rates than other proteins at BDV-infected tissues. In this study, BDV-p24 protein was constructed and subcloned into expression plasmid pET22. Confirmation of recombinant protein expression was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blotting. P24 protein was injected into rabbits with the aim of polyclonal antibody production and immunization. Compared to other diagnostic methods, ELISA is a fast method with cost effective and high sensitivity as well as lower probability of contamination. ELISA method was performed to evaluate the infection in laboratory rabbits and retrospective infection was examined in 50 rabbits. The obtained results in this study indicated that the ELISA method based on p24 protein has a high potential to detect BDV infection.

کلیدواژه‌ها

Borna disease virus؛ ELISA؛ Polyclonal antibody؛ Borna-P24 protein؛ Diagnostic method

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