Effect of Cytokine Treatment on Spring Scion Stimulation of Taracco Scion on Citromelo Rootstock

علوم باغبانی

Volume 38, Issue 4 - Serial Number 64, December 2024, Pages 775-763   

Document Type: Research Article

DOI: 10.22067/jhs.2024.87914.1343

Authors

S.M Gholami; M. Hadadinejad^* ; H. Moradi; H. Sadeghi

Department of Horticulture, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran

Abstract

Introduction
Bud dormancy is one of the important issues in planting and cultivation of fruit trees that needs to be addressed in many trees such as orange (Citrus sinensis). Bud dormancy involves cessation of horizontal and vertical growth, lack of budbreak, and reduction in plant activity during cold weather. One of the commercial orange cultivars is Tarocco blood orange which compared to older blood cultivars, is larger in size and with lower alternate bearing has higher marketability. The nursery trees of this cultivar are grafted on sour orange, citrange, and vigorous rootstock of citrumelo (a hybrid between trifoliate orange and grapefruit) (Talon et al., 2020). One of the major problems of nurserymen in spring grafting of Tarocco cultivar on vigorous citrumelo rootstock is the failure of about 50 percent of buds to break compared to other cultivars on the same rootstock and other similar rootstocks. This unwanted dormancy leads to a one-year delay in the nursery tree production process and unnecessary occupation of nursery space. Given the commercial importance of blood orange and the adverse effects of bud dormancy on yield and fruit lifetime, solutions are used to control and overcome this problem. One of the effective solutions is the application of cytokinins which can stimulate the growth of graft buds (Yadav & Saini, 2018).
 
Materials and Methods
This research was conducted in a citrus nursery at the University of Agricultural Sciences and Natural Resources of Sari, in late May 2022. Citrumelo seedlings were grown in 5.3 liter pots containing a loamy-silt soil mix in the nursery location. Tarocco cultivar buds were prepared from a seven-year-old mother orchard and T-budding was performed in June (during rootstock bark slipping). All hormone treatments were applied after graft union and before bending the branch using a soft brush on the graft buds. The applied treatments included hormone treatment (control, 5000 mg.l^-1 benzyladenine, 1000 mg.l^-1 kinetin, and 50 mg.l^-1 thidiazuron) and treatment time (13, 15, and 17 days after grafting). After two months, some traits related to budbreak and growth of the grafted buds were evaluated.
 
Results and Discussion
The results showed that thidiazuron and benzyladenine treatments had better effects compared to kinetin treatment on spring budbreak and initial growth of Tarocco grafted buds. In a way that 50 mg.l^-1 thidiazuron treatment had the highest number of sprouted buds (67.91), largest leaf area (118.04 cm²), highest number of leaves (16.50), especially when applied 13 days after grafting. Also, in leaf size related traits, leaf area indices, graft growth rate as well as chlorophyll and carotenoid content of Tarocco graft leaves were significantly affected by different hormonal treatments and application times, with 50 mg.L^-1 thidiazuron being more effective than other treatments. Cytokinins can promote division and expansion of leaf cells and thereby result in increased cell numbers and improvement of different leaf parameters. Also, cytokinins regulate important physiological processes like photosynthesis. Application of these materials provides cell division especially in areas like buds and growth points and also possibly more buds may form on the spring graft by using these treatments during the grafting process which can lead to increased bud break and faster plant growth (Cook & Bahar, 2017). Increasing cytokinin levels can stimulate the photosynthesis process which results in increased food production, leaf growth and ultimately increased leaf area. On the other hand, cytokinins affect plant metabolism and can regulate production and accumulation of different growth factors. This may lead to a better balance in nutrient distribution and metabolic activities which in turn aids leaf area increase (Hodchek et al., 2023). Finally, according to the obtained results, it can be recommended to nurserymen of this cultivar to use 50 mg.L^-1 thidiazuron 13 days after grafting as a practical and effective strategy for increasing spring budbreak, growth and development of Tarocco buds grafted on citrumelo.
 
Conclusions
In general, based on the results, the application of 50 mg.l^-1 of thidiazuron 13 days after grafting can be considered a practical and effective strategy to enhance bud awakening, as well as the growth and development of Tarocco spring shoots on citronmelo. This practice is recommended for producers of seedlings of this cultivar.

Keywords

Benzyl adenine; Blood orange; Bud dormancy; Thidiazoron

References

• Albrigo, L.G., Stelinski, L.L., & Timmer, L.W. (2019). Citrus, 2nd Edition. CABI, 314 p.
• Arnon, D.T. (1949). Copper enzymes in isolation chloroplast phenoloxidase in Beta vulgaris. Plant Physiology, 24, 1-15. https://doi.org/10.1104/pp.24.1.1
• Bidabadi, S.S., Afazel, M., & Sabbatini, P. (2018). Iranian grapevine rootstocks and hormonal effects on graft union, growth and antioxidant responses of Asgari seedless grape. Horticultural Plant Journal, 4(1), 16-23.‏ (In Persian)
• Bozsó, Z., & Barna, B. (2021). Diverse effect of two cytokinins, kinetin and benzyladenine, on plant development, biotic stress tolerance, and gene expression. Life, 11(12), 1404. https://doi.org/10.3390/life11121404
• Brenner, W.G., Ramireddy, E., Heyl, A., & Schmülling, T. (2012). Gene regulation by cytokinin in Arabidopsis. Frontiers in Plant Science, 3, 8. https://doi.org/10.3389%2Ffpls.2012.00008
• Chamani, E., Ghasemnejad, M., & Geraylo, S. (2013). An Introduction to the Physiology of Flowering Plants. Mohaghegh Ardabili University Press, Ardabil, Iran. 26 pp. (In Persian)
• Di Benedetto, A., Galmarini, C., & Tognetti, J. (2015). Effects of combined or single exogenous auxin and/or cytokinin applications on growth and leaf area development in Epipremnum aureum. The Journal of Horticultural Science and Biotechnology, 90(6), 643-654. https://doi.org/10.1080/14620316.2015.11668727
• Dobránszki, J., & Mendler-Drienyovszki, N. (2014.) Cytokinin-induced changes in the chlorophyll content and fluorescence of in vitro apple leaves. Journal of Plant Physiology, 171(16), 1472-1478. https://doi.org/10.1016/j.jplph.2014.06.015
• Ghanem, M.E., Albacete, A., Smigocki, A.C., Frébort, I., Pospísilová, H., Martínez-Andújar, C., Acosta, M., Sánchez-Bravo, J., Lutts, S., Dodd, I.C., & Pérez-Alfocea, F. (2011). Root-synthesized cytokinins improve shoot growth and fruit yield in salinized tomato (Solanum lycopersicum) plants. Journal of Experimental Botany, 62(1), 125-140. https://doi.org/10.1093/jxb/erq266
• Giron, D., Kaiser, W., Imbault, N., & Casas, J. (2007). Cytokinin-mediated leaf manipulation by a leafminer caterpillar. Biology Letters, 3(3), 340-343. https://doi.org/10.1098%2Frsbl.2007.0051
• Glanz-Idan, N., Lach, M., Tarkowski, P., Vrobel, O., & Wolf, S. (2022). Delayed leaf senescence by upregulation of cytokinin biosynthesis specifically in tomato roots. Frontiers in Plant Science, 13, 922106. https://doi.org/10.3389/fpls.2022.922106
• Gonzalez, N., Vanhaeren, H., & Inze, D. (2012). Leaf size control: Complex coordination of cell division and expansion. Trends in Plant Sciences, 17, 332-340. https://doi.org/10.1016/j.tplants.2012.02.003
• Gujjar, R.S., Banyen, P., Chuekong, W., Worakan, P., Roytrakul, S., & Supaibulwatana, K. (2020). A synthetic cytokinin improves photosynthesis in rice under drought stress by modulating the abundance of proteins related to stomatal conductance, chlorophyll contents, and rubisco activity. Plants, 9(9), 1106. https://doi.org/10.3390/plants9091106
• Hayat, F., Li, J., Liu, W., Li, C., Song, W., Iqbal, S., Khan, U., Javed, H.U., Altaf, M.A., Tu, P., Chen, J., & Liu, J. (2022). Influence of citrus rootstocks on scion growth, hormone levels, and metabolites profile of ‘Shatangju’mandarin (Citrus reticulata Blanco). Horticulturae, 8(7), 608. https://doi.org/10.3390/horticulturae8070608
• Hollobone, J. (2020). Propagation Techniques for Flowers, Vegetables, and Trees: Growing Plants from Seeds, Cuttings, Grafts, Division, and Bulbs. Company, Incorporated, 192 p.
• Holst, K., Schmulling, T., & Werner, T. (2011). Enhanced cytokinin degradation in leaf primordia of transgenic Arabidopsisplants reduces leaf size and shoot organ primordia formation. Journal of Plant Physiology, 168, 1328-1334. https://doi.org/10.1016/j.jplph.2011.03.003
• Hudeček, M., Nožková, V., Plíhalová, L., & Plíhal, O. (2023). Plant hormone cytokinin at the crossroads of stress priming and control of photosynthesis. Frontiers in Plant Science, 13, 1103088. https://doi.org/10.3389%2Ffpls.2022.1103088
• Jamshidi, A., Ebrahimi, M.A., Rajabian, T., Bakhshi khaniki, Gh.R., & Mozaffari, S. (2018). Study the effects of auxins and cytokinins on growth, pigments and protein contents of Chlorella sorokiniana. Journal of Plant Research, 31(2), 303-315. (In Persian)
• Jogaiah, S., & Porika, H. (2023). Scion preconditioning and cytokinin treatment improved graft compatibility in Red Globe grape grafted on Dogridge rootstock. Indian Journal of Horticulture, 80(2), 165-170.‏
• Kalate Jari, S., Khalighi, A., Moradi, F., & Fattahi Moghaddam, M.R. (2009). The effects of cytokinins, sucrose and 8-hydroxyquinoline sulfate on the longevity and postharvest quality of cut rose flowers var. Red Gant. Iranian Journal of Horticultural Science (IJHS), 39(1), 125-135. (In Persian)
• Kim, D.H., & Sivanessan, I. (2016). Influence of benzyladenine and thidiazuron on shoot regeneration from leaf and shoot tip explants of Sedum sarmentosum Brazilian Archives of Biology and Technology, 59, 16150717. https://doi.org/10.1590/1678-4324-2016150717
• Lorteau, M.A., Ferguson, B.J., & Guinel, F.C. (2001). Effects of cytokinin on ethylene production and nodulation in pea (Pisum sativum) cv. Sparkle. Physiologia Plantarum, 112(3), 421-428. https://doi.org/10.1034/j.1399-3054.2001.1120316.x
• Mangena, P. (2022). Evolving role of synthetic cytokinin 6-benzyl adenine for drought stress tolerance in soybean (Glycine max Merr.). Frontiers in Sustainable Food Systems, 6, 992581. https://doi.org/10.3389/fsufs.2022.992581
• Martin, F.J. (2020). Plant Propagation Book: The Process of Creating New Plants. Independently Published, 84 p.
• Maxwell, B.B., & Kieber, J.J. (2005). Cytokinin signal transduction. In Plant hormones: biosynthesis, signal transduction, action. Dordrecht: Springer Netherlands.‏ 329-357.
• Mehmood, M., Pérez-Llorca, M., Casadesús, A., Farrakh, S., & Munné-Bosch, S. (2021). Leaf size modulation by cytokinins in sesame plants. Plant Physiology and Biochemistry, 167, 763-770. https://doi.org/10.1016/j.plaphy.2021.09.013
• Morris, J.W., Doumas, P., Morris, R.O., & Zaerr, J.B. (1990). Cytokinins in vegetative and reproductive buds of Pseudotsuga menziesii. Plant Physiology, 93(1), 67-71. https://doi.org/10.1104/pp.93.1.67
• Niedz, R.P., & Bowman, K.D. (2023). Improving citrus bud grafting efficiency. Scientific Reports, 13(1), 17807. https://doi.org/10.1038/s41598-023-44832-x
• Nielsen, J. M., Brandt, K., & Hansen, J. (1993). Long-term effects of Thidiazuron are intermediate between benzyladenine, kinetin or isopentenyladenine in Miscanthus sinensis. Plant Cell, Tissue and Organ Culture, 35, 173-179. https://doi.org/10.1007/BF00032967
• Osugi, A., & Sakakibara, H. (2015). Q&A: How do plants respond to cytokinins and what is their importance?. BMC Biology, 13(1), 1-10. https://doi.org/10.1186/s12915-015-0214-5
• Peyvandi, M., Kazemi, L., & Majd, A. (2016). Effect of different cytokinins on micropropagation of Lavandula vera. Journal of Plant Research, 28(2), 257-263. (In Persian). https://doi.org/28204
• Saboora, A., & Shokri, M. (2014). Effect of plant growth regulators on in vitro germination and micropropagation of brazmbl (Perovskia abrotanoides), a medicinal plant. Iranian Journal of Plant Biology, 5(18), 95-114. (In Persian).
• Sadeghi, F., Sohrabi, Y., & Sio-Se Mardeh, A. (2022). Effect of plant growth regulators on soluble carbohydrates, photosynthetic pigments and chlorophyll fluorescence of Sirvan and Homa wheat cultivars in rainfed and irrigation conditions. Journal of Crop Production and Processing, 12(3), 81-100. (In Persian). http://dx.doi.org/10.47176/jcpp.12.3.36862
• Salek Mearaji, H., Tavakoli , A., & Sepahvand, N.A. (2021). Evaluating the effect of cytokinin foliar application on morphological traits and yield of quinoa (Chenopodium quinoa) under optimal irrigation and drought stress conditions. Journal of Crop Ecophysiology, 14(56), 479-478. (In Persian). https://doi.org/10.30495/jcep.2021.679976
• Shoja, H.M., & Shishavan, H.K. (2021). Effects of different hormonal treatments on growth parameters and secondary metabolite production in organ culture of Hyssopus officinalis BioTechnologia, 102(1), 33-41. https://doi.org/10.5114%2Fbta.2021.103760
• Skalák, J., Vercruyssen, L., Claeys, H., Hradilová, J., Černý, M., Novák, O., Plačková, L., Saiz-Fernández, I., Skaláková, P., Coppens, F., Dhondt, S., Koukalová, Š., Zouhar, J., Inzé, D., & Brzobohatý, B. (2019). Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis. Plant Journal, 97(5), 805-824. https://doi.org/10.1111/tpj.14285
• Sosnowski, J., Truba, M., & Vasileva, V. (2023). The impact of auxin and cytokinin on the growth and development of selected crops. Agriculture, 13(3), 724. https://doi.org/10.3390/agriculture13030724
• Tabeta, H., Gunji, S., Kawade, K., & Ferjani, A. (2023). Leaf-size control beyond transcription factors: Compensatory mechanisms. Frontiers in Plant Science, 13, 1024945. https://doi.org/10.3389/fpls.2022.1024945
• Talon, M., Caruso, M., & Gmitter, J.F.G. (2020). The Genus Citrus. Woodhead Publishing, Elsevier Inc. All rights reserved. 538 p.
• Tan, M., Li, G., Chen, X., Xing, L., Ma, J., Zhang, D., Ge, H., Han, M., Sha, G., & An, N. (2019). Role of cytokinin, strigolactone, and auxin export on outgrowth of axillary buds in apple. Frontiers in Plant Science, 10, 616. https://doi.org/10.3389/fpls.2019.00616
• Tanavardi, S., Tafazoli, E.A., Panahi, B., & Samiei, Kh. (2013). Investigating the effect of using different concentrations of kinetin and bending the stem on stimulating the bud growth of Miyagawa mandarin scion. 6th National Conference on Watershed Management and Soil and Water Resources Management, Kerman, Iran. (In Persian)
• Wu, W., Du, K., Kang, X., & Wei, H. (2021). The diverse roles of cytokinins in regulating leaf development. Horticulture Research, 8(1), 1-13. https://doi.org/10.1038/s41438-021-00558-3
• Xiong, D., Wang, D., Liu, X., Peng, S., Huang, J., & Li, Y. (2016). Leaf density explains variation in leaf mass per area in rice between cultivars and nitrogen treatments. Annals of Botany, 117(6), 963-971.‏ https://doi.org/10.1093/aob/mcw022
• Yadav, R.K., & Saini, P.K. (2018). Plant hormones: Their nature occurrence and functions: A chapter. European Journal of Biotechnology and Bioscience, 6(6), 13-17.
• Zavaleta-Mancera, H.A., López-Delgado, H., Loza-Tavera, H., Mora-Herrera, M., Trevilla-García, C., Vargas-Suárez, M., & Ougham, H. (2007). Cytokinin promotes catalase and ascorbate peroxidase activities and preserves the chloroplast integrity during dark-senescence. Journal of Plant Physiology, 164(12), 1572-1582. https://doi.org/10.1016/j.jplph.2007.02.003
• Zubo, Y.O., Yamburenko, M.V., Selivankina, S.Y., Shakirova, F.M., Avalbaev, A.M., Kudryakova, N.V., Zubkova, N.K., Liere, K., Kulaeva, O.N., Kusnetsov, V.V., & Börner, T. (2008). Cytokinin stimulates chloroplast transcription in detached barley leaves. Plant Physiology, 148(2), 1082-1093. https://doi.org/ 10.1104/pp.108.122275