Evaluation of the Tolerance of Promising Genotype of Bean (Phaseolus vulgaris Linnaeus) to Two-Spotted Mite (Tetranychus urticae Koch (Acari: Tetranychidae)) in Field Conditions

پژوهش‌های حبوبات ایران

Volume 15, Issue 2 - Serial Number 30, December 2024, Pages 301-315   

Document Type: Original Article

DOI: 10.22067/ijpr.2024.86974.1085

Authors

Sedighe Ashtari^{* 1}; Behrouz Asadi²; Hamid Reza Dorri²

¹Plant Protection Research Department, Markazi Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization, AREEO, Arak, Iran

²Crop and Horticultural Science Research Department, Markazi Agricultural and Natural Resources Research and Education Center Research and Education Center (AREEO), Arak, Iran

Abstract

Introduction
Legumes are one of the rich sources of plant protein after cereals and the second most important food source for humans. The most important biotic stresses that lead to reduced growth, performance, and sometimes the death of plants are pests, diseases, and weeds. The two-spotted spider mite, due to its extensive host range, rapid population growth, and ability to develop resistance to pesticides, is one of the key pests. Therefore, the use of tolerant varieties for damage control is considered as most reliable, healthy, and cost-effective method in an integrated management system. The feeding of this mite on bean (Phaseolus vulgaris Linnaeus) leaves leads to the formation of yellow spots on the leaf surface (Dorri et al., 2015). The activity of two-spotted spider mite Tetranychus urticae Koch, is mainly associated with webbing, which leads to the accumulation of dust, decreasing of the photosynthesis and increasing the pest damage (Hosseini, 2018). Therefore, due to the importance of this pest, this research was conducted to identify resistant genotypes of beans among promising bean genotypes.
 
Materials and Methods
For the experiments, Type 1 promising bean genotypes (KS-21216, KS-21181, KS-21538, KS-21565, KS-21563, KS-21602, KS-21500, KS-21601, KS-21600, and KS-21607), Type 2 genotypes (KS-21573, KS-21597 and KS-21606), and Type 3 genotypes (KS-21255 and KS-21574) along with a susceptible genotype Sadri were cultivated in a field at Khomein National Bean Research Station in a randomized complete block design with three replications during 2022-03. Sampling for damage assessment was carried out weekly from the third leaf stage until the end of the season. From each plot, 10 plants were selected randomly. Two leaves, one from the bottom and the other from the top of each plant, were selected and scored (on a scale of 1-6). To assess performance and its components, field cultivation was conducted under the specified conditions in two separate plots. One plot was treated with pesticide spraying, while the other remained untreated. Characteristics such as pod number per plant, seed number per pod, plant height, and weight of one hundred seeds were measured under both mite-infested and non-infested conditions. Based on the performance of the genotypes under T. urticae mite-infested and non-infested conditions, productivity indices, geometric mean productivity, stress tolerance, and stress sensitivity were calculated.
 
Results and Discussion
The yield and yield components of the genotypes under the presence and absence of T. urticae were evaluated. Genotype KS-21538 had the highest (2166.67 kg.ha^-1) and the sensitive check had the lowest performance (995.17 kg.ha^-1). The highest (16.24) and lowest (4.88) percentage of yield reduction were related to the sensitive check and genotype KS-21538, respectively. The highest and lowest number of pods per plant were observed in genotype KS-21538 and Sadri cultivar with 17.95 and 8.87 pods, respectively. The highest and lowest damage scores under unsprayed conditions were 3.06 and 4.56 in genotype KS-21538 and Sadri respectively. The results of calculating the indices showed that the highest efficiency index, average geometric efficiency index, and stress tolerance index were observed in genotypes KS-21538 and KS-21216, respectively, and the lowest observed in the Sadri and genotype KS-21607. The lowest stress sensitivity index was observed in genotypes KS-21538 and KS-21216, respectively, and the highest observed in Sadri and genotype KS-21607. Researchers examined how planting density and different bean varieties (Akhtar, Derakhshan, and 285) affect the population of two-spotted spider mites. They discovered that the pest density on the Akhtar variety is higher than on Derakhshan and 285. Due to the differences in the bean varieties studied in this research and the current study, the results do not show similarities. The results of this study also showed that pest density is significantly influenced by the bean variety (Karimi et al., 2019). In a study, it was revealed that the Derakhshan, Akhtar, and local Khomein (Sadri) varieties were sensitive to T. urticae, while Dorsa and Kousha varieties and lines 21191 and 31169 showed greater resistance to the two-spotted spider mite and also had acceptable yield compared to other varieties (Kazemi et al., 2019). In the present study, the Sadri variety was also identified as sensitive to damage by the two-spotted spider mite, which is consistent with these results.
 
Conclusions
According to the results genotypes KS-21538 and KS-21216 are recommended as the first and second priority for cultivation in the region due to the higher yield, lower percentage of yield reduction, and the lower damage scale.

Keywords

Damage scale; Indices; Population changes; Yield

References

Abolfathi, N., Kocheili, F., & Mohiseni, A. (2018). Investigating the most suitable sampling unit and space for the two-spotted tartan mite population Tetranychus urticae Koch in common bean fields Phaseolus vulgaris L. in the north of Lorestan province. Plant Protection, 34(2), 33-45.

Ahmadi, K., Ebadzadeh, H., Hatami, F., Abdshah, H., & Kazemian, A. (2022). Agricultural statistics, the first volume of crops. Ministry of Jihad Agriculture, Bureau of Statistics and Information Technology. 99 p.

Ahmadi, M., Fathipour, K., & Kamali, K. (2006). Growth parameters of Tetranychus urticae Koch on different bean cultivars. Iranian Journal of Entomology, 26(2), 10-1.

Alabi, O. Y., Odebiyi, J. A., & Tamo, M. (2004). Effect of host plant resistance in some cowpea (Vigna unguiculata L.) cultivars on growth and developmental parameters of the flower bud thrips, Megalurothrips sjostedti (Trybom). Crop Protection, 23, 83–88. https://doi.org/10.1016/S0261-2194(03)00171-6

Ashtari, S., Yousefi, M., & Dorri, H. R. (2019). Evaluation of the resistance of new bean genotypes to two-spotted mite in field and greenhouse conditions, Applied Researches in Plant Protection, 9(2), 15-30.

Ashtari, S., & Ghadiri, A. (2019). Chiti bean cultivars resistant to two-spotted mite. Beans, 2(4), 1-10.

Aydemir, M., & Torus, S. (1992). The effect of different bean varieties on the life duration and egg productivity of Tetranychus urticae. Proceeding of 2nd Turkish National Congress of Entomology. Jan 28-31, Izmir, Turkey. p. 145-155.

Baradaran, B., Arbabi, M., & Shaiei, R. (2004). Evaluation of the difference between different eggplant cultivars on populations of Tetranychus urticae in Varamin region. 16^th Iranian Congress of Plant Protection. 258 pp.

Dent, D. (2000). Host plant resistancein Dent, D. editors. Insect pest management CABI Publishing, Wallingford, Oxfordshire, United Kingdom, pp. 123-179. https://doi.org/10.1079/9780851993409.0123

Dewar, A. M., Haylock, L. A., Bean, K. M., Garner, B. H., & Boyce, R. (2000). The ecology and control of the two-spotted spider mite, Tetranychus urticae, in sugar beet. Proceedings of the Brighton Crop Protection Conference Pests and Diseases, 3, 913-918.

Dorri, H., Asadi, B., Ghadiri, A., Lak, M. H., Yousefi, M., Ghanbari, A. A. Baizaei, A., Kamel, M. Kushki, M. H., Asterki, H., Pourmatin, R., & Hatemabadi Farahani, M. (2015). Ghaffar, a new variety of chiti beans. Scientific Journal of Research Findings in Agricultural and Garden Plants, 5(2), 155-143.

Dorri, H. R., & Ardeh, M. J. (1999). Final report of a field evaluation of two-spotted spider mite resistance in bean genotypes. Center for Agricultural and Natural Resources Research, 36 p.

Fernandez, G. C. (1993). Effective selection criteria for assessing plant stress tolerance. pp. 257-270. In: C. G. Kuo (Ed.) Adaptation of Food Crops to Temperature and Water Stress. AVRDC, Shunhua, Taiwan. https://doi.org/10.22001/wvc.72511

Fischer, R. A., & Maurer, R. (1978). Drought resistance in spring wheat cultivars. I. Grain yield responses. Australian Journal of Agricultural Research, 29, 897-912. https://doi.org/10.1071/AR9780897

Flexner, J. L., Westigard, P. H., Hilton, R., & Croft, B. A. (1995). Experimental evaluation of resistance: management for two spotted spider mite on southern Oregon pear: 1987–1993. Journal of Economic Entomology, 88(6), 1517-1524. https://doi.org/10.1093/jee/88.6.1517

Hosseini, A. A. (2018). Implementation guide genotypes for the management of two-spotted mite. Plant Protection Organization, 11 pp.

Impe, G. V., & Hance, T. (1993). A technique for testing varietal susceptibility in the mite (Tetranychus urticae) application to bean, cucumber, tomato, strawberry. Agronomic, 13(8), 739-749. https://doi.10.1051/agro:19930806

Karimi, A., Yarahmadi, F., & Mohseni Amin, A. (2019). Effects of bean plant density and its different cultivars on population of two spotted spider mite, Tetranychus urticae and onion thrips, Thrips tabaci in field conditions, Plant Pest Research, 9(2), 39-48. https://doi.10.22124/iprj.2019.3622

Kazemi, A. A., Askarianzadeh, A., Saidizadeh, A., & Ghadiri, A. (2019). Evaluation of the resistance of bean cultivars to the two-spotted mite in field conditions. Iranian Legume Research, 11(2), 95-108. https://doi. 10.22067/ijpr.v11i2.76080

Kirisik, M., Erler, F., Boyaci, F., & Bayram, Y. (2021). Evaluation of resistance in 16 eggplant genotypes to the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Phytoparasitica, 49, 275–285. https://doi.10.1007/s12600-020-00856-x

Knapp, M., Mugada, D. A., Agong, S. G., & Knapp, M. (2003). Screening tomato (Lycopersicon esculentum) accessions for resistance to the two-spotted spider mite: Population growth studies. Insect Science and its Application, 23, 15-19. https://doi.org/10.1017/S1742758400012200

Lima, P. F., Colombo, C. A., Chiorato, A. F., Yamaguchi, L. F., Kato, M. J., & Carbonell, S. A. (2014). Occurrence of isoflavonoids in Brazilian common bean germplasm Phaseolus vulgaris L. Journal of Agricultural and Food Chemistry, 62, 969-970. https://doi.10.1021/jf5033312

Mohammadi, S., Seraj, A. A., Saeidi, Z., & Moharramipour, S. (2012). Evaluation of resistance and susceptibility of 14 red and white bean genotypes to two-spotted spider mite, Tetranychus urticae Koch. (Acari: Tetranychidae). Plant Protection Scientific Journal of Agriculture, 33(2), 11-24.

Mohiseni, A., Koushki, M. H., & Astraki, H. (2016). Investigating the Effect of Planting Arrangement, plant density on Population density and Severity of damage to Spider Mites, Final Project Report. Boroujerd Agricultural and Natural Resources Research and Education Campus, 28 p.

Pedigo, L. P. (2002). Entomology and Pest Management. Iowa University press, Iowa, USA. 646 p.

Rosielle, A. A., & Hamblin, J. (1981). Theoretical aspect of selection for yield in stress and non-stress environments. Crop Science, 21, 943-946.

Rui, S., Hua, W., Rui, G., Qin, L., Lei, P., Jianan, L., Zhihui, H., & Chanyou, C. (2016). The diversity of four anti-nutritional factors in common bean. Horticultural Plant Journal, 2(2), 97-104. https://doi.org/10.1016/j.hpj.2016.06.001

Saeidi, Z. (2020). Screening of 55 pinto bean genotypes for resistance to the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), Persian Journal Acarology, 9(3), 291-299.

Saeidi, Z., Babaei, G., & Salehi, F. (2020). Population density and yield loss caused by two spotted spider mite, Tetranychus urticae Koch on different pinto bean lines under field Conditions. Systematic and Applied Acarology, 25(12), 2212–2223.

Saeidi, Z., & Arbabi, M. (2014). Comparing the effects of several acaricides in controlling different densities of two-spotted mite populations in bean fields in Lordegan region. Journal of Pesticides in Plant Protection Science, 2(2), 82-73.

Saeidi, Z., & Salehi, F. (2014). Studying the resistance of selected genotypes from the local population of Chiti Lordegan bean to the two-spotted tartan mite. Plant Pests and Diseases, 73(1), 65-77.

Saeidi, Z., & Arbabi, M. (2007). Effectiveness of 12 pesticides against two infestation levels of bean fields by Tetranychus urticae Koch in Lordegan, Chaharmahal and Bakhtiari province. Journal of Pajouhesh and Sazandegi, 76, 25-31.

Sarfraz, M., Dosdall, L. M., & Keddie, B. A. (2006). Diamondback moth-host plant interactions: implications for pest management. Crop Protection, 25, 625-630. https://doi.10.1016/j.cropro.2005.09.011

Shaabani, J., Hossainzadeh, A., Zeinali, H., & Naghavi, M. R. (2021). A field study on common. 140, 464–476. https://doi.org/10.1111/pbr.12914

Shoorooei, M., Hoseinzadeh, A. H., Maali-Amiri, R., Allahyari, H., & Torkzadeh-Mahani, M. (2018). Antixenosis and antibiosis response of common bean (Phaseolus vulgaris) to two spotted spider mite (Tetranychus urticae). Experimental and Applied Acarology, 74, 365–381. https://doi. 10.1007/s10493-018-0240-4

Sirjani, M., & Arbabi, M. (2004). Investigating of difference of pollution of cultivars in introducing of cotton to to Tetranychus urticae. in Kashmar. 16^th Iranian Congress of Plant Protection. pp. 270.

Smith, C. (2005). Plant resistance to arthropods, molecular and conventional approaches. 423 pp. Springer Publisher.

Tahmasebi, Z. (2013). Determination of susceptible and susceptible cultivars to bean spider mites using resistance index. 6^th Conference on Agricultural Research Findings, 95 pp.

Taleei, A., Mohammadi, A., Maali Amiri, R., & Khanjani, M. (2020). Screening of common bean accessions to two- spotted spider mite (Tetranychus urticae Koch) under growth chamber condition. Journal of Biology, 10(7), 1-10. https://doi.10.4172/2322-0066.10.7.006

Yousefi, M., & Dorri, H. (2012). Evaluation of the mechanisms of resistance of 36 genotype of pinto beans spider mites in greenhouse and field and green house conditions. Final Project Report, Central Province Agricultural and Natural Resources Research Center, 53 p.