Effects of trona (sodium sesquicarbonate) on physio-biochemical profiles and cardiovascular risk indices in Wistar rats

Iranian Journal of Veterinary Science and Technology

دوره 16، شماره 4 - شماره پیاپی 37، اسفند 2024، صفحه 38-48   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2024.85999.1335

نویسندگان

Emeka Hillary Ugwuanyi^{* 1}؛ Obinna Benedict Onyejekwe²؛ Moniloye Zakiru Akanfe³

¹Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka.

²Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka

³Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka.

چکیده

Purpose of the study: This investigation aimed to assess the impact of trona on the cardiovascular risk markers and physio-biochemical profiles of rats administered graded dosages over 28 days. Five groups (n = 5) of rats—A, B, C, D, and E—were randomly assigned, with E acting as the control. For 28 days, rats in groups A, B, C, and D were administered trona at doses of 50, 100, 200, and 400 mg/kg, correspondingly. All over the investigation, distilled water was provided to the animals in the control group. We measured body weights, oxidative stress, hematology, hepatorenal profiles, somatic organs, fasting blood glucose (FBG) levels, and cardiovascular risk indices (CVRI). Findings: Rats administered 400 mg/kg had higher FBG levels (p < 0.05) than the control group. Group D showed decreases (p < 0.05) in erythrocytic indices, total protein level, and heart-body index with concurrent increases (p < 0.05) in total leucocyte counts (TLC) and creatinine levels in comparison to the control group. Rats in groups B, C, and D showed higher levels of catalase activity (p < 0.05) compared to the control group. Rats given 100 mg/kg or above of trona showed increases (p < 0.05) in CVRI compared to controls. Conclusions: There were dose-dependent harmful effects on the erythrocytic indices, high exposure to T2DM (type-2 diabetes mellitus), and increased CVRI levels in rats dosed orally with ≥100 mg/kg trona for 28 days.

کلیدواژه‌ها

Cardiovascular indices؛ hepatorenal profiles؛ oxidative stress؛ rats؛ trona

مراجع

1.    Imafidon KE, Egberanmwen ID, Omoregie IP. Toxicological and biochemical investigation in rats administered “kaun” (trona) a natural food additive used in Nigeria. J Nutr Intermed Metab. 2016; 6, 22-25. 
2.    Ajibo TO, Komolafe YO, Yakubu MT, Ogunbode SM.  Effects of trona on the redox status of cellular system of male rats. Toxicol Ind Health. 2015; 31 (2), 179-189. doi: 10.1177/0748233712469654
3.    Lu WY, Zhang T, Zhang DY, Li CN.  A novel bioflocculant. Biochem Eng J. 2005; 27(1), 1–7.
4.    Copenhafer WC, Maleskas E, inventors GA, Wyoming LP. Method of reducing the formation of scale in the production of soda ash from trona and nahcolite ores. United States patent US 6022516A. 2000.
5.    Aribido SO, Ogunmodede BK, Lakpini CAM. Nutritional assessment of ‘Gwanwarasa’. Type of natural potash (kanwa). Niger J Chem Res. 2001; 6 (3), 27-30. 
6.    Witkowska D, Slowik J, Chilicka K. Heavy metals, and human health: possible exposure pathways and the competition for protein binding sites. Molecules. 2021; 7: 26(19), 6060. Doi: 10.3390/molecules26196060.
7.    Joint FAO/WHO. Food Standards Programme Codex Committee on contaminants in foods. In 5th session. 2014. Available at: ftp://ftp.fao.org/codex/meeting/CCCF/ccf5/cf05/NF.pdf. [Accessed 4 February 2023]. 
8.    Westman EC. Type 2 diabetes mellitus: a pathophysiologic perspective. Front Nutr. 2021; 8: 707371. Doi: 10.3389/fnut.2021.707371.
9.    Ajayi AF, Akhigbe RE. The antispermatogenic mechanism of trona is associated with lipid peroxidation but not testosterone suppression. J Hum Reprod Sci. 2017. 10: 124-7.
10.    He F, Zuo L. Redox roles of reactive oxygen species in cardiovascular diseases. Int J Mol Sci. 2015; 16: 27770-80. https: //doi.org/10.3390/ijms161126059.
11.    Plant J, Smitt D, Smitt B, Williams L. 2000. Environmental geochemistry at the global scale. J Geol Soc. 2000; 157(4): 837-49. 
12.    Satoh T, Nakagawa K, Sugihara F, Kuwahara R, Ashihara M, Yamane F, et al. Identification of an atypical monocyte and committed progenitor involved in fibrosis. Nature. 2017; 541 (7635): 96-101.
13.    Berrington JE, Barge D, Fenton AC, Cant AJ, Spickett GP. Lymphocyte subsets in term and significantly preterm UK infants in the first year of life were analyzed by single-platform flow cytometry. Clin Exp Immunol. 2005; 140: 289-92.
14.    Erlinger S, Arias IM, Dhumeaux D. 2014. Gastroenterology. 2014; 46:1625-38.
15.    Famurewa AC, Ekeleme-Egedigwe CA, Nwali SC, Agbo NN, Obi JN, Ezechukwu GC. Dietary Supplementation with Virgin Coconut Oil Improves Lipid Profile and Hepatic Antioxidant Status and Has Potential Benefits on Cardiovascular Risk Indices in Normal Rats. J Diet Suppl. 2018;15(3):330-342. Doi: 10.1080/19390211.2017.1346031. 
16.    Nandi A, Yan LJ, Jana CK, Das N. Role of catalase in oxidative stress- and age-associated degenerative disease. Oxid Med Cell Longev. 2019; 9613090. Doi: 10.1155/2019/9613090.
17.    Cardelle-Cobas A, Cristina Soria A, Corzo-Martínez M, Villamiel M. A comprehensive survey of garlic functionality. In: Pacurar M, Krejci G, editors. Garlic consumption and health. Spain (MAD), Nova Science. 2010.
18.    Gasevic D, Frohlich J, Mancini GB, Lear SA. Clinical usefulness of lipid ratios to identify men and women with metabolic syndrome: a cross-sectional study. Lipids Health Dis. 2014; 13: 159.
19.    Ingelsson E, Schaefer E, Contois JH, McNamara JR, Sullivan L, Keyes MJ, et al. Clinical utility of different lipid measures for prediction of coronary heart disease in men and women. JAMA. 2007; 298: 776-85.
20.    Rizzo M, Berneis K. Low-density lipoprotein size and cardiovascular risk assessment. QJM. 2006; 99: 1-14.
21.    Yusuff OT, Kolawole BA, Ikem RT, Soyoyo DO, Amjo OO. Cardiovascular risk indices and their impact on outcome in patients with hyperglycaemic emergencies in Nigerian Hospital. J Natl Medical Assoc. 2020; 112 (1): 28-35. Doi: 10.1016/j.jnma.2019.12.004.
22.    Zhang L, Yang H, Yang P. The Correlation between Type 2 Diabetes Mellitus and Cardiovascular Disease Risk Factors in the Elderly. Appl Bionics Biomech. 2022;2022:4154426. Doi: 10.1155/2022/4154426. 
23.    Association of Official Analytical Chemists (AOAC). In: Williams S, editor. Official methods of analysis of the Association of Official Analytical Chemists. 14th ed. USA Washington (DC): Arlington. 1984; 38-64.  
24.    Tietz NW. Clinical guide to laboratory tests, 3rd ed. United States (PHL): Saunders; 1995; 268-273. 
25.    Skoog DA, Holler FJ, Crouch SR. Principles of instrumental analysis, 6th ed. USA Belmont (CA): Thomson Brooks/Cole; 2007; 150.
26.    NIH Publication. Respect for life. National Institute of Environment. Health. Sci. NIEHS. http://www.niehs.gov/oc/factsheets/wri/studybgn. 1985; 85-23. [Accessed 2022 November 20].
27.    OECD. Test No. 423: Acute oral toxicity - acute toxic class method, Organization for Economic Cooperation and Development (OECD) guidelines for the testing of chemicals, section 4. France (PAR): OECD Publishing. 2002. Doi:10.1787/9789264071001-en. [Accessed 2022 April 12].
28.    Zarei L, Shahrooz R. Protective effects of Cornus mas fruit extract on methotrexate-induced alterations in mice testicular tissue: Evidence for histochemical and histomorphometric changes in an animal model study. Vet Res Forum. 2019; 10 (4): 307-313. Doi:10.30466/vrf.2019.69516.1955. 
29.    Thrall MA, Weiser MG. Hematology. In: Hendrix, C.M., ed. Laboratory Procedures for Veterinary Technicians, 4th ed. USA Mosby (MO). 2002; 29-74. 
30.    Higgins T, Beutler E, Doumas BT. Measurement of hemoglobin in blood. In: Burtis, C.A., Ashwood, E.R., Bruns, D.E., eds. Fundamentals of Clinical Chemistry, 6th ed. United States (MO): Elsevier. 2008; 524-525. 
31.    Hadwan MH. Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC Biochem. 2018; 19 (7): 1-8. Doi:10.1186/s12858-018-0097-5  
32.    Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351-58. Doi:10.1016/0003-2697(79)90738-3.
33.    Abbortt RD, Wilson PW, Kannel WB, Castelli WP. High-density lipoprotein cholesterol, total cholesterol screening, and myocardial infarction: The Framingham study. Arteriosclerosis. 1988; 8: 207-11.
34.    Alladi S, Khada A, Shanmugan M. Induction of hypercholesterolemia by simple soil protein with acetate generating amino acid. Nutr Rep Int. 1989; 40, 893-94.