Investigating the Effect of Chicken Manure and Potassium Sulfate on the Physiological Properties of Fenugreek )Trigonella foenum-graecum L.( Medicinal Plant

بوم شناسی کشاورزی

Article 1, Volume 16, Issue 4 - Serial Number 62, December 2025, Pages 587-604   

Document Type: Research Article

DOI: 10.22067/agry.2024.85755.1175

Authors

Mohammad hossein Aminifard^{* 1}; Sajjad Nadafa²; Hassan Hassan²; Mehdi Jahani³

¹Department of Horticultural Sciences and Regional Plant Research Center, Faculty of Agriculture, Birjand University, Birjand, Iran.

²Department of Horticultural Sciences, Faculty of Agriculture, Birjand University, Birjand, Iran,

³Department of Plant Pathology, Faculty of Agriculture, University of Birjand, Birjand, Iran

Abstract

Introduction
Fenugreek (Trigonella foenum-graecum L.), is an annual plant found in many countries in Africa, Asia, and Europe, and India is the largest producer in the world. There are two types of fenugreek, one botanically known as Trigonella foenum-gracum and the other Trigonella corniculata (Champa or Kasturi Methi) of the Leguminosae family. The integrated management system is a system in which reducing the use of chemical fertilizers and the combined use of chemical fertilizers and organic materials are considered. Among organic fertilizers, chicken manure is very important due to its high nitrogen concentration, high carbon quality, low carbon-nitrogen ratio, and providing large amounts of important nutrients required for plant growth. The appropriate amount of potassium sulfate fertilizer in the soil facilitates the osmotic adjustment in the soil, as a result of which the osmotic pressure remains in the leaves, and in this way, the plant's ability to cope with drought stress increases. Therefore, considering the mentioned cases and the lack of scientific information in the field of combining fertilizers, in this research, an attempt was made to find a suitable fertilizer combination consisting of chicken manure and potassium sulfate for the fenugreek plant.
Materials and Methods
To investigate the effect of chicken manure and potassium sulfate on the physiological characteristics of the fenugreek medicinal plant, factorial research was conducted based on randomized complete block design with three replications in the crop year 2021-2022 in Qochan city in the north of Razavi Khorasan province. Experimental treatments included chicken manure at three levels (0, 1000, and 2000 kg. h^-1) and potassium sulfate at four levels (0, 1.5, 3, and 5 per thousand). The chicken manure concentrate was given to the ground according to the investigated area before planting. The planting depth of fenugreek seeds was considered to be 1.5 cm. To prevent the binding of seeds, the seeds were covered with fine sand mixed with animal manure. Then, at the 4-6 leaf stage, thinning was done to reach the appropriate density. The first irrigation after planting and subsequent irrigations were done once every six days until the end of the growing season. Also, Soluptas fertilizer was applied as a foliar spray after planting, from the six-leaf stage to the flowering stage, three times with an interval of 14 days. The investigated traits of this experiment included morphological and physiological traits, which were sampled after the plants were fruited and the pods turned yellow by removing the marginal effect.
Results and Discussion
The results showed that the highest level of secondary and main stem height, leaf dry weight and other growth traits were obtained with the highest level of chicken manure (2000 kg/ha), which of course was not significantly different from the level of 1000 kg/ha of chicken manure. The use of chicken manure, especially in arid and semi-arid areas, which mainly have low organic matter levels, in addition to increasing soil organic matter and availability of nutrients, can improve the physical, chemical, and biological characteristics of these soils. Also, the highest amount of growth traits was obtained with the use of the highest level of Soluptas, i.e. five per thousand. In the interaction effect of two investigated fertilizers, it was observed that the interaction of organic and chemical fertilizers caused a double effect on the investigated growth traits. The level of 2000 kg of chicken manure caused a 16.38% increase in the weight of a thousand fenugreek seeds compared to no fertilization. The weight of 1,000 seeds was 10.408 gr at the 5 per thousand Soluptas level and 8.906 gr at the non-fertilization level. Also, the interaction of chicken manure 2000 kg/ha with each level of Soluptas caused the highest number of pods in the fenugreek plant. The highest levels of both treatments caused an increase of 83.76% and 93.28% of seed and biological yield compared to no fertilization (control level).
Conclusion
In general, it can be concluded that among the simple effects, the highest amount of traits was related to chicken manure, and among the investigated levels in two treatments, the highest yield of fenugreek dry matter was obtained with the consumption of 2000 kg/ha of chicken manure and 5 per thousand Soluptas.

Keywords

harvest index; organic fertilizer; pods; Soluptas

References

1. Aasim, M., Baloch, F., Nadeem, M., Bakhsh, A., Sameeullah, M., & Day, S. (2018). Fenugreek (Trigonella foenum-graecum ): An underutilized edible plant of modern world. Global Perspectives on Underutilized Crops, 381-408. https://doi.org/10.1007/978-3-319-77776-4_12 
2. Abbasi, M.K., Khaliq, A., Shafiq, M., Kazmi, M., & Ali, I. (2010). Comparative effectiveness of urea N, poultry manure and their combination in changing soil properties and maize productivity under rainfed conditions in Northeast Pakistan. Experimental Agriculture, 46(2), 211-230. https://doi.org/10.1017/s0014479709991050 
3. Adim, G., Malekzadeh, E., Dordipour, E., Kiani, F., Mokhtarpour, H., & Moazzemi, S. (2022). Evaluation of the integrated effect of chemical and bio-organic fertilizers on yield and yield components of rapeseed. Journal of Agricultural Engineering Soil Science and Agricultural Mechanization, (Scientific Journal of Agriculture), 45(2), 119-135. (In Persian). https://doi.org/10.22055/AGEN.2022.40631.1632
4. Al-Taai, S.H.H. (2021). The effect of fertilizer uses on environmental pollution: A review. Review of International Geographical Education Online, 11(5), 3620-3529.
5. Alizadeh, P., Fallah, S., & Raiesi, F. (2012). Potential N mineralization and availability to irrigated maize in a calcareous soil amended with organic manures and urea under field conditions. International Journal of Plant Production, 6(4), 493-512. https://doi.org/10.22069/IJPP.2012.762
6. Allahvirdizadeh, N., & Deljou, M.N. (2014). Effect of humic acid on morph-physiological traits, nutrients uptake and postharvest vase life of pot marigold cut flower (Calendula officinalis Crysantha) in hydroponic system. Journal of Science Technology of Greenhouse Culture, 5(18), 133-143. (In Persian). https://dorl.net/dor/20.1001.1.20089082.1393.5.2.12.6
7. Azizi, M., Soltani, A., & Khavari Khorasani, S. (2008). Canola (physiology, agronomy, breeding and biotechnology). Jihad University of Mashhad Press, Mashhad, Iran. 230 p. (In Persian)
8. BS, E. (2005). Effect of poultry dung and cattle manure on chemical properties of clay and sandy clay loam soil. Journal of Animal and Veterinary Advances, 4(10), 839-841.
9. Chen, J.H. (2006). The combined use of chemical and organic fertilizers and/or biofertilizer for crop growth and soil fertility. Paper presented at the International workshop on sustained management of the soil-rhizosphere system for efficient crop production and fertilizer use.
10. ElNour, M.E., Ali, A.M., & Saeed, B. (2015). Antimicrobial activities and phytochemical screening of callus and seeds extracts of fenugreek (Trigonella foenum-graecum). International Journal of Current Microbiology Applied Sciences, 4(2), 147-157.
11. Esfahani, M., Sadrzadeh, S.M., Kavoosi, M., & Dabagh-Mohammadi-Nasab, A. (2005). Study the effect of different levels of nitrogen and potassium fertitizers on yield, yield components and growth of rice c. v. Khazar. Iranian Society of Crops and Plant Breeding Sciences, 7(3), 226-241. (In Persian). http://dorl.net/dor/20.1001.1.15625540.1384.7.3.4.0
12. Esmaeilian, Y., & Jalali, A. (2022). Evaluation of quantitative, qualitative, and economic aspects of garlic (Allium sativum) and fenugreek (Trigonella foenum-graceum L.) intercropping under organic and chemical nutrition systems. Iranian Journal of Medicinal and Aromatic Plants Research, 38(1), 133-149. (In Persian with English abstract). (In Persian). https://doi.org/10.22092/ijmapr.2022.354814.3003
13. Fallah, S., Ghalavand, A., & Raiesi, F. (2013). Soil chemical properties and growth and nutrient uptake of maize grown with different combinations of broiler litter and chemical fertilizer in a calcareous soil. Communications in Soil Science Plant Analysis, 44(21), 3120-3136. https://doi.org/10.1080/00103624.2013.832284 
14. Ghalenoei, M., Moafporian, G., & Drostkar, M. (2014). Effect of potassium and zinc application on yield and yield components in normal irrigation and deficit irrigation. Journal of Plant Ecophysiology, 6(18), 55-68. (In Persian). https://doi.org/1001.1.20085958.1393.6.18.5.4
15. Ghanbari, S., Moradi Telavat, M., & Siadat, S.A. (2017). Evaluation of competitive indices in barley intercropped with fenugreek under manure applications. Journal of Crops Improvement, 18(4), 821-834. (In Persian). https://doi.org/10.22059/jci.2017.56654
16. Gopalan, C., Rama Sastri, B., & Balasubramanian, S. (2004). Nutritive value of Indian foods. National Institute of Nutrition, ICMR, Hyderabad.
17. Griffe, P., Metha, S., & Shankar, D. (2003). Organic production of medicinal, aromatic and dye yielding plants (MADPs): forward, preface and introduction. Food Agriculture Organization, 2, 52-63.
18. Hanway, J.J., & Johnson, J.W. (1985). Potassium nutrition of soybeans. In: Potassium in agriculture R.D. Munson (Ed.). ASA-CSSA-SSSA. 753-764.
19. Hossain, M.D., Musa, M.H., Talib, J., & Jol, H. (2010). Effects of nitrogen, phosphorus and potassium levels on kenaf (Hibiscus cannabinus ) growth and photosynthesis under nutrient solution. Journal of Agricultural Science, 2(2), 49-57. https://doi.org/10.5539/jas.v2n2p49 
20. Houshyarifard, M., & Qaranchik, A. (2009). Effect of source and rate of manures on incidence and severity of important diseases, yield and yield components in cotton (Gossypium hirsutum). Iranian Society of Crops and Plant Breeding Sciences, 11(3), 237-248.
21. Ibrahim, F.R., & Helaly, A. (2017). Growth and productivity response to nitrogen, potassium and humic acid of fenugreek (Trigonella foenum-graecum ) plant. Middle East Journal of Agriculture Research, 6(4), 1526-1535.
22. Jasim, A.H., Ali, A.H., & Lilo, S.A. (2016). Effect of organic and chemical soil fertilizers and their interactions with foliar fertilizer on some vegetative growth of fenugreek. Annales of West University of Timisoara. Series of Biology, 19(2), 199-206.
23. Javanmard, A., & Shekari, F. (2016). Improvement of seed yield, its components and oil content of sunflower (Helianthus annuus) by applications of chemical and organic fertilizers. Journal of Crop Ecophysiology, 10(37 (1)), 35-56. (In Persian).
24. Jianrong, F., Changgeng, Z., Lina, J., & Zheng, W.J. (1995). Potassium improves yield and quality of mulberry leaves. Beter Crops, 79(4).
25. Karimi Zinkanlou, S., Hemti, K., & Khairkhah, M. (2015). The effect of different levels of organic fertilizers on the morphophysiological traits of Ziziphora clinopodioides. Plant Production Research Journal, 23(4), 1-16. http://dx.doi.org/10.12988/asb.2015.519
26. Maghsudi, E., Ghalavand, A., & Aghaalikhan, M. (2014). The effect of different levels of fertilizer (organic, biological and chemical) on morphological traits and yield of maize single cross hybrid 704. Applied Field Crops Research, 27(104), 129-135. (In Persian). http://doi.org/22092/AJ.2014.101820
27. Mandal, S., & DebMandal, M. (2016). Fenugreek (Trigonella foenum-graecum) oils. In Essential oils in food preservation, flavor and safety (pp. 421-429): Elsevier. https://doi.org/10.1016/b978-0-12-416641-7.00047-x 
28. Marschner, H. (1995). Mineral nutrition of higher plants 2^nd Institute of Plant Nutrition University of Hohenheim: Germany.
29. Mehrafarin, A., Naghdi Badi, H., Mirzai Motlagh, M., Salehi, M., & Ghiasi Yekta, M. (2017). Phytochemical and morphophysiological responses of dill (Anethum graveolens ) to foliar application of potassium sulfate and methanol biostimulant. Journal of Medicinal Plants, 16(64), 93-108.
30. Mirhashemi, S.M., Kouchaki, A.R., Parsa, M., & Nassiri Mahallati, M. (2009). Evaluating the benefit of ajowan and fenugreek intercropping in different levels of manure and planting pattern. Iranian Journal of Field Crops Research, 7(1), 271-281.
31. Mirlouhi, A.F., Mohammadi, R., Razavi, S.J., & Nourbakhsh, F. (2008). Effect of different fertilizer applications during rice and corn cultivation on barley yield in a three years replication. Journal of Sustainable Agriculture and Production Science, 18(3), 161-171. (In Persian).
32. Naser, A.A. (2017). Study of response Trigonella foenum-graecum to spraying with high potassium (Miller) and high phosphor (Agroleaf). Tikrit Journal of Pure Science, 22(9), 6-10. https://doi.org/10.25130/tjps.v22i9.866 
33. Noorgholipour, F., Samavat, S., & Tehrani, M. (2019). The necessity of combined application of chemical and organic fertilizers in sustainable agricultural systems. Paper presented at the The First Congress of Fertilizer Challenges in Iran: Half a Century of Fertilizer Consumption, Tehran, Iran.
34. Pandey, V., Patel, A., & Patra, D. (2016). Integrated nutrient regimes ameliorate crop productivity, nutritive value, antioxidant activity and volatiles in basil (Ocimum basilicum). Industrial Crops Products, 87, 124-131. https://doi.org/10.1016/j.indcrop.2016.04.035 
35. Pandey, V., & Patra, D. (2015). Crop productivity, aroma profile and antioxidant activity in Pelargonium graveolens L’Hér. under integrated supply of various organic and chemical fertilizers. Industrial Crops Products, 67, 257-263. https://doi.org/10.1016/j.indcrop.2015.01.042 
36. Perramon, B., Bosch-Serra, A., Domingo, F., & Boixadera, J. (2016). Organic and mineral fertilization management improvements to a double-annual cropping system under humid Mediterranean conditions. European Journal of Agronomy, 76, 28-40. https://doi.org/10.1016/j.eja.2016.01.014 
37. Rezae, M., & Baradaran, R. (2013). Effects of bio fertilizers on the yield and yield components of pot marigold (Calendula officinalis ). Iranian Journal of Medicinal Aromatic Plants Research, 29(3), 635-650. (In Persian). https://doi.org/10.22092/ijmapr.2013.4047
38. Rostaei, M., & Fallah, S. (2016). Assessment of canopy characteristics and essential oil yeild of fenugreek and black cumin in intercropping under application of organic and chemical ferilizer. Journal of Agricultural Science and Sustainable Production, 25(4), 1-23. (In Persian).
39. Rostaie, M., Falah, S., & Souraki, A. (2015). Effect of fertilizer sources on growth, yield and yield components of fenugreek intercropped with black cumin. Journal of Crop Production, 7(4), 222-197. (In Persian). http://doi.org/1001.1.2008739.1393.7.4.11.0
40. Sadrzadeh, M., & Moalemi, N. (2005). The effect of water and potassium stress on the vegetative characteristics of young olive seedlings, Zard cultivars and Baghmolek. Agricultural Research, 6(4), 1-10.
41. Sakinejad, T. (2003). Study of effect of water deficit on the trend of uptake of N, P, K and Na at different growth stages considering the morphological and physiological traits of maize in Ahvaz climate.D. Thesis on Crop Physiology, Science and Research Branch, Ahvaz, Iran,
42. Salehi, A., Fallah, S., Abasi Sourki, A., & Tadayon, M.R. (2017). Evaluation of yield and yield components of fenugreek (Trigonella foenum-graecum) and buckwheat (Fagopyrum esculentum Moench) under organic and chemical fertilizers. Iranian Journal of Medicinal and Aromatic Plants Research, 33(2), 338-352. (In Persian). https://doi.org/10.22092/ijmapr.2017.106802.1804
43. Scharf, P.C., Kitchen, N.R., Sudduth, K.A., Davis, J.G., Hubbard, V.C., & Lory, J.A. (2005). Field‐scale variability in optimal nitrogen fertilizer rate for corn. Agronomy Journal, 97(2), 452-461. https://doi.org/10.2134/agronj2005.0452 
44. Seilsepour, M. (2014). Study of potassium and zinc effects on quantity and quality parameters and grain cadmium content of winter wheat irrigated by waste water. Applied Field Crops Research, 27(104), 96-105. (In Persian). http://doi.org/22092/AJ.2014.101683
45. Shafii Zargar, A. (2015). Investigating the quantitative and qualitative characteristics of green cucumber according to organic and mineral substances in autumn cultivation. M.Sc. thesis, Tarbiat Modares University, Tehran, Iran.
46. Shirvanipour, L., Panahi Kordloghari, K., & Kalidri, A.p. (2013). Investigating the effect of different levels of potassium sulfate on yield and yield components of three varieties of mung bean. Paper presented at the The Second National Conference on Medicinal Plants and Sustainable Agriculture. Hamedan. Hegmatane Environmental Assessor Association, Iran.
47. Siadat, S.A., & Moradi-Telavat, M.R. (2018). Practical Aspects of Organic Farming. Tehran: Agricultural Education and Extension Press, 500 p. (In Persian).
48. Tahami, S., Rezvani Moghaddam, P., & Jahan, M. (2010). Comparison the effect of organic and chemical fertilizers on yield and essential oil percentage of basil (Ocimum basilicum ). Journal of Agroecology, 2(1), 63-74. (In Persian). http://doi.org/10.22067/JAG.V2I1.7603
49. Uwah, D.F., Ogar, C.C., & Akpan, R.J. (2014). Effects of poultry manure and plant population on soil properties and agronomic performance of sweet maize (Zea mays Saccharata Strut). International Journal of Applied, 4(4), 1-20.
50. Vedpathak, M., & Chavan, B. (2016). Fertilizers effects on growth and yield components of fenugreek vegetable (Trigonella foenum-graecum ) in a field trial. International Journal for Innovative Research in Science Technology, 3(7), 180-183.
51. Wani, S.A., & Kumar, P. (2018). Fenugreek: A review on its nutraceutical properties and utilization in various food products. Journal of the Saudi Society of Agricultural Sciences, 17(2), 97-106. https://doi.org/10.1016/j.jssas.2016.01.007 
52. Zhao, J., Ni, T., Li, J., Lu, Q., Fang, Z., Huang, Q., Zhang, R., Li, R., Shen, B., & Shen, Q. (2016). Effects of organic–inorganic compound fertilizer with reduced chemical fertilizer application on crop yields, soil biological activity and bacterial community structure in a rice–wheat cropping system. Applied Soil Ecology, 99, 1-12. https://doi.org/10.1016/j.apsoil.2015.11.006