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Saidian, S., Farahmand, S., Fathi, M., Saifi, S. (2024). Improving Growth Performance, Biochemical Parameters and Reducing Mortality due to Ascites in Broilers by Propranolol Inclusion. , 16(4), 517-528. doi: 10.22067/ijasr.2024.88379.1201
Shahriar Saidian; Somaya Farahmand; Mokhtar Fathi; Salar Saifi. "Improving Growth Performance, Biochemical Parameters and Reducing Mortality due to Ascites in Broilers by Propranolol Inclusion". , 16, 4, 2024, 517-528. doi: 10.22067/ijasr.2024.88379.1201
Saidian, S., Farahmand, S., Fathi, M., Saifi, S. (2024). 'Improving Growth Performance, Biochemical Parameters and Reducing Mortality due to Ascites in Broilers by Propranolol Inclusion', , 16(4), pp. 517-528. doi: 10.22067/ijasr.2024.88379.1201
Saidian, S., Farahmand, S., Fathi, M., Saifi, S. Improving Growth Performance, Biochemical Parameters and Reducing Mortality due to Ascites in Broilers by Propranolol Inclusion. , 2024; 16(4): 517-528. doi: 10.22067/ijasr.2024.88379.1201

Improving Growth Performance, Biochemical Parameters and Reducing Mortality due to Ascites in Broilers by Propranolol Inclusion

پژوهشهای علوم دامی ایران
Article 5, Volume 16, Issue 4 - Serial Number 60, December 2025, Pages 517-528    PDF (725.29 K)
Document Type: Research Articles
DOI: 10.22067/ijasr.2024.88379.1201
Authors
Shahriar Saidian1; Somaya Farahmand1; Mokhtar Fathi* 2; Salar Saifi1
1Department of Biology, Payame Noor University, Tehran, Iran
2Department of Animal Sciences, Payame Noor University, Tehran, Iran
Abstract
Introduction: Pulmonary hypertension syndrome (ascites) is one of the most common metabolic syndromes in today's fast-growing broilers. It is associated with rapid growth and is characterized by the accumulation of fluid in the abdominal spaces. Effective factors in the occurrence of ascites syndrome included free radicals in the body such as superoxide, hydroxide, and hydrogen peroxide. By reducing the capacity of the body's antioxidant systems, free radicals make the bird susceptible to various diseases. Free radicals are produced in the body by damaging the cell membrane leading to cell death and ultimately tissue damage. Therefore, by increasing cell protection from such injuries, one of the common causes of ascites and heart failure abnormalities can be prevented. In addition, reactive oxygen species (ROS) reduce the half-life of nitric oxide (vasodilating agent), causing a decrease in the ability of vasodilation and providing the basis for the occurrence of ascites. B-blockers (or b-adrenergic antagonists) are a group of drugs widely used in the treatment of cardiovascular diseases, namely arterial hypertension, cardiac arrhythmias, and angina pectoris as well as other types of pathologies such as anxiety or glaucoma. Part of the beneficial cardiovascular effects shown by this group of compounds has already been associated with the antioxidant properties that some of them seem to possess. It demonstrated a concentration-dependent membrane anti-peroxidative activity for propranolol, pindolol, metoprolol, atenolol, and sotalol. Of the five b-blockers examined, propranolol was the most potent agent, and the activities seemed to correlate with the drugs’ hydrophobicity. Further studies indicated that the antioxidant activity of propranolol was independent of its pharmacological activity and was related to its intrinsic chemical properties rather than to its quinidine-like membrane stabilization effect. The antioxidant properties of propranolol were later corroborated using membrane and cellular models. More recently, propranolol’s antioxidant-related cardioprotective effects were studied in rats, showing that the chronic treatment with this b-blocker was found able to protect against ischemia-reperfusion injury. Thus, it is now accepted that propranolol is an important therapeutic tool against oxidative stress by stabilizing membranes, including lysosomes, inducing the activity of antioxidant and other beneficial enzymes, increasing endothelial nitric oxide production, and directly protecting isolated membranes, cardiovascular cells, and tissues against oxidative injury. So far, there have been few studies on the effects of propranolol in broilers. Therefore, the current research was designed to evaluate the propranolol on the growth performance, antioxidant status, and the status of some hematological and biochemical parameters of broiler chickens under induced ascites.
 
Materials and Methods: The experiment was carried out in a completely randomized design with four treatments in four replicates and 10 chickens in each replicate. Experimental treatments included: 1- positive control group (no induce ascites, basal diet), 2- negative control group (inducing ascites and fed with basic diet), 3 and 4- propranolol groups (inducing ascites, supplemented with 50 and 100 ppm propranolol). To induce ascites, water containing 7 g per liter of salt was provided to the chickens from day 15 of age. Growth performance parameters including feed intake, weight gain, and feed conversion ratio were calculated for the total period. On the last day of the experiment (42 d), two birds were randomly selected from each cage and after sampling from the wing vein, they were killed and the ascites index was calculated as the ratio of the weight of the right ventricle to the weight of total ventricles (RV/TV). The mortalities were collected as soon as they were observed and after weighing to correct the feed conversion ratio, were necropsied to investigate the cause of death. Serum antioxidant parameters include the level of malondialdehyde and the activity of antioxidant enzymes including glutathione peroxidase, superoxide dismutase, and catalase were determined. In addition, the serum enzymes including alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured.
Results and Discussion: The results showed that induction of ascites increased RV/TV, mortality due to ascites, and feed conversion ratio; while it decreased feed intake, and live weight (P<0.05). Additionally, ascites induction increased the number of white and red blood cells, hematocrit, hemoglobin, heterophile, and decreased lymphocytes (P<0.05). The levels of glutathione peroxidase, superoxide dismutase, and catalase enzymes were decreased in ascites-induced broiler chickens. At the same time, it decreased the level of serum malondialdehyde, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase enzymes (P<0.05). Inclusion of propranolol had no significant effect on growth performance (P>0.05), but it significantly decreased the ratio of right ventricle to total ventricles and ascites related-mortalities (P<0.05). Moreover, inclusion of propranolol reduced the number of white and red blood cells, hematocrit, hemoglobin, heterophile and increased lymphocytes compared to the negative control birds (P<0.05). In addition, propranolol enhanced the levels of glutathione peroxidase, superoxide dismutase and catalase enzymes and reduced the levels of malondialdehyde, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in serum (P<0.05). In conclusion, propranolol inclusion at 100 ppm may be used to alleviation the physiological responses of broilers exposed to ascites.
Conclusion: In conclusion, propranolol inclusion at 100 ppm may be used to alleviation the physiological responses of broilers exposed to ascites.

 
Keywords
Antioxidant; Ascites; Broiler chickens; Propranolol; Pulmonary hypertension syndrome; Salt
References
  1. Arab, H., Jamshidi, A., Rassuli, R., Shams, G., & Hassanzadeh, M. H. (2006). Generation of hydroxyl radicals during ascites experimentally induced in broilers. British Poultry Science, 47, 216-222. http://dx.doi.org/1080/00071660600611102.
  2. Bautista-Ortega, J., & Ruiz-Feria, C. A. (2010). L-arginine and antioxidant vitamins E and C improve the cardiovascular performance of broiler chickens grown under chronic hypobaric hypoxia. Poultry Science, 89, 2141-2146. http://dx.doi.org/3382/ps.2010-00764.
  3. Bryson, P. (1997). Comprehensive review in toxicology for emergency clinicians (3 Ed.). Washington, DC: Taylor & Francis. p. 167. ISBN9781560326120. Archived from the original on 24 March 2017
  4. Davidson, J. R. (2006). Pharmacotherapy of social anxiety disorder: What does the evidence tell us? The Journal of Clinical Psychiatry, 67 (12), 6-20. http://dx.doi.org/1016/j.genhosppsych. 2005.07.002. PMID 17092192.
  5. Daneshyar, M., Kermanshahi, H., & Golian, A. (2009). Changes of biochemical parameters and enzyme activities in broiler chickens with cold-induced ascites. Poultry Science, 88, 108-110. http://dx.doi.org/3382/ps.2008-00170.
  6. Fathi, M., Tanha, T., Taherkhani, R., & Razaee Komasi, V. (2023). Comparative study of the effects of ethanolic extract of sage (Salvia officinalis) and vitamin C on antioxidant status, blood and biochemical parameters, mortality, and performance in broilers under induced ascites. Animal Production Research, 12 (3), 15-27. http://dx.doi.org/22124/AR.2023.23780.1751 (In Persian).
  7. Fathi, M., Tanha, T., & Saeedyan, S. (2022). Influence of dietary lycopene on growth performance, antioxidant status, blood parameters and mortality in broiler chicken with cold-induced ascites. Archive Animal Nutrition, 8, 1-11. http://dx.doi.org/1080/1745039X.2022.2046451.
  8. Fathi, M., Haydari, M., & Tanah, T. (2016a). Influence of dietary aspirin on growth performance, antioxidant status, and mortality due to ascites in broiler chickens. Poultry Science Journal, 4(2), 139-146. http://dx.doi.org/ 22069/PSJ.2016.10701.1178.
  9. Fathi, M., & Haydari, T. (2016b). Effects of atenolol on growth performance, mortality due to ascites, antioxidant status and some blood parameters in broilers under induced ascites. Iranian Journal of Animal Science Research, 8 (2), 329-339. http://dx.doi.org/22067/IJASR.V8I2.47188.(In Persian).
  10. Fischer, J., & Ganellin, C. R2006). Analogue-based Drug Discovery. John Wiley & Sons. p. 463. ISBN 9783527607495.
  11. Frautschy, S. A., Hu, , Kim, P., Miller, S. A., Chu, T., Harris-White, M. E., & Cole, G. M. )2001(. Phenolic anti-inflammatory antioxidant reversal of Ab-induced cognitive deficits and neuropathology. Neurobiology of Aging, 22(6), 993–1005.
  12. Gomes, A., David Costa, Jose´ L. F. C. Lima & Fernandes, E. (2006). Antioxidant activity of b-blockers: An effect mediated by scavenging reactive oxygen and nitrogen species? Bioorganic and Medicinal Chemistry, 14(13), 4568–4577. https://doi.org/10.1016/j.bmc.2006.02.023.
  13. Hassanzadeh, M. (2017). Metabolic disorder of poultry. 3th Tehran University Press, Tehran, Iran.
  14. Hassanzadeh, M., J. Buyse & E. Decuypere. (2002). Further evidence for the involvement of cardiac beta-adrenergic receptors in right ventricle hypertrophy and ascites in broiler chickens. Avian Pathology, 31(2), 177-81.http://dx.doi.org/1080/03079450120118676.
  15. Julian, R. J. (1993). Ascites in poultry. Avian Pathology, 22 (3), 419-454, http://dx.doi.org/1080/03079459308418934.
  16. Khaper, N., Claudio, R., Charita, S., Timao, L., & Pawan K. S. (1997). Chronic treatment with propranolol induces antioxidant changes and protects against ischemia–reperfusion injury. Journal of Molecular and Cellular Cardiology, 29(12), 3335-3344. http://dx.doi.org/1006/jmcc.1997.0558
  17. Kramer, J.H., Spurney, C. F., & Iantorno, M. (2012). D-Propranolol protects against oxidative stress and progressive cardiac dysfunction in iron overloaded rats. Canadian Journal of Physiology and Pharmacology, 90 (9), 1257–68. https://doi. org/10.1139/y2012-091.
  18. Lorenzoni, A. G., & Ruiz-Feria, C. A. (2006). Effects of vitamin E and l-arginine on cardiopulmonary function and ascites parameters in broilers chickens reared under sub-normal temperatures. Poultry Science, 85, 2241–2250. http://dx.doi.org/1093/ps/85.12.2241.
  19. Luger, D., Shinder, D., Rzepakovsky., V., Rusal., M., & Yahav, S. (2001). Association between weight gain, bloodparameters, and thyroid hormones and the development of ascites syndrome in broiler chickens. Poultry Science, 80, 965-971.
  20. Mak, T., & William, B. W. (2004). Potent antioxidant properties of 4-hydroxyl-propranolol. the Journal of Pharmacology and Experimental Therapeutics, 308 (1), 58032/1116386. http://dx.doi.org/1124/jpet.103.058032.
  21. Mirsalimi, S. M., O'Brien, P. J., & Julian, R. J. (1992) Blood volume increase in salt-induced pulmonary hypertension, heart failure and ascites in broiler and white leghorn chickens. Canadian Journal of Veterinary Research, 57(2), 110–113.
  22. Nemati, M. H., Amanlou, F., & Shahir, M. A. (2022). Effect of adding Mentha piperita powder on performance, immune system, and blood parameters of broilers under ascites induction conditions. Animal Production Research, 11(1), 27-38. http://dx.doi.org/ 22124/AR.2022.19209.1603. (In Persian).
  23. Nooreh, Z., Taherpour, K., Akbari Gharaei, M., Shiraz, H., & Ghasemi, H. A. (2020). Comparing effects of Ferulago angulate extract with antibiotic, probiotic, and vitamin-mineral complex on growth performance and immune response of broiler chickens exposed to heat stress. Journal of Animal Production, 22(4), 645-657. http://dx.doi.org/ 22059/jap.2020.304657.623540. (In Persian).
  24. Olfati, A., Mojtahedin, A., Sadeghi, T., Akbari, M., & Martínez-Pastor, F. (2018). Comparison of growthperformance and immune responses of broiler chicks reared under heat stress, cold stress and thermoneutralSpanish Journal of Agricultural Research, 16 (2), e0505-e0505. https://doi.org/10.5424/sjar/2018162-12753.
  25. Ranasinghe, H. N., N. Fernando, S., Handunnetti , P. N. Weeratunga, P. Katulanda, S. Rajapakse, P. Galappatthy & Constantine, G. R. (2020). The impact of propranolol on nitric oxide and total antioxidant capacity in patients with resistant hypertension—Evidence from the APPROPRIATE trial. BMC Research Notes, 13, 228. https://doi.org/10.1186/s13104-020-05067-5.
  26. Ramos, B. P., Colgan, L., Eric N. S., Ovadia, , Wilson, R., Amy, F T., & Arnsten, F.T. (2005(. The beta-1 adrenergic antagonist, betaxolol, improves working memory performance in rats and monkeys. Biological Psychiatry, 58(11), 894–900.
  27. Rajani, J., Karimi Torshizi, M. A., & Rahimi, S. H. (2011). Control of ascites mortality and improved performance and meat shelf-life in broilers using feed adjuncts with presumed antioxidant activity. Animal Feed Science and Technology, 170, 239– 245. http://dx.doi.org/1016/j.anifeedsci.2011.09.001.
  28. Serreau, P., Amirouche, A., Benyamina, A., & Berteina-Raboin, S. (2024). Propranolol hydrochloride psychiatric effectiveness andoxidative stress: An update. Oxygen, 4, 139–149. https://doi.org/10.3390/oxygen4020009.
  29. Venkata, S., & Ram, M.D. (2010). Beta-blockers in hypertension. The American Journal of Cardiology, 106, 1819–1825.
  30. Walldorf, J., Hillebrand, C., Aurich, H.,   Stock, P., Hempel, , Ebensing, S., Wolfgang, E Fleig, T. S., Matthias, M. D,. & Bruno C. ) 2010(. Propranolol impairs liver regeneration after partial hepatectomy in C57Bl/6-mice by transient attenuation of hepatic lipid accumulation and increased apoptosis. Scandinavian Journal of Gastroenterology, 45(4), 468–476. http://dx.doi.org/10.3109/00365520903583848

 

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