The investigation of Leptin gene Promoter in the Najdi Cows

پژوهشهای علوم دامی ایران

Article 6, Volume 6, Issue 1 - Serial Number 17, March 2014   

Document Type: Genetics & breeding

DOI: 10.22067/ijasr.v6i1.22328

Authors

fatemeh amraei^* ; Hedayatollah roshanfekr¹; Jamal Fayazi; Mohammad Bojarpour²

¹Department of Animal Science, University of Khuzestan Agricultural Sciences and Natural Resources, Khuzestan, Iran.

²Department of Animal Sciences, Faculty of Animal Sciences and Food Industry, University Agriculture and Natural Resources Ramin Khuzestan, Khuzestan, Iran.

Abstract

Identification of associated genes with energy balance, yield and feed intake are recent interests of the animal breeding researchers. Najdi breed is the famous cattle breed in the Khuzestan province. In this research for The investigation of Leptin gene Promoter ,from 15 Najdi cows in the Shushtar cattle center station was taken. DNA from whole blood was Extracted and 544bp and 566 bp two pieces of was used for amplification. After ensuring the correctness PCR products on agarose gel 1% sequences gene was performed and component promoter leptin was found in Najdi cow. In the first fragment(566bp) of the study was recognized, 6 Mutation, two point mutations one of the transition and the other was transversion and four frameshift mutations that mutations of another typ of, deletion and insertion. In the second segment(544bp( was observed in the leptin gene promoter region two point mutations one of the transition and the other was transversion and deletion of a frameshift Mutation The results of this study showed that reports Mutations on boxes and component promoter did not affect leptin gene.In this study was compared the sequence corresponding to a single on sample in terms of both pieces were registered on sequence similarity with other region that blast results showed a high percentage of similarity.

Keywords

leptin gene; PCR; Promoter; Mutation