Effect of Different Light Sources on Morphology and Growth of Potato Plantlets under in-vitro Conditions and their Effect on Minituber Production in Greenhouse

علوم باغبانی

Article 4, Volume 31, Issue 4, November 2017, Pages 683-693   

Document Type: Research Article

DOI: 10.22067/jhorts4.v31i4.57458

Authors

Jafar Nabati^{* 1}; Elahe Boroumand Rezazadeh¹; Mohammad Zare Mehrjerdi²; Mohammad Kafi¹

¹Ferdowsi University of Mashhad

²Agriculture Faculty of Shirvan, University of Bojnord, Bojnord, Iran

Abstract

Introduction: Conventional seed programmes take more than 10 years and diseases during each round of field multiplication become increasingly common and especially those transmitted through seed tubers. In contrast, the production of large volume of propagation material in protected environments requires only a few additional years of traditional seed multiplication in the field to produce the desired seed with an improved health status. This is useful especially in countries where there are no vector-free production areas for producing high quality potato seed tubers. In the last few decades, alternative seed production programmers have been developed in which the first multiplication steps are speeded up using in vitro plantlets, microtubers or minitubers. In the area of controlled environment agriculture (CEA), one of the most commonly discussed topics is lighting. The lighting system is generally the most expensive component of a controlled environment agriculture facility in terms of upfront costs as well as ongoing expenses (electricity use as well as replacement lamps). Naturally, advances in lighting technology are of great interest to the controlled environment agriculture community for these very reasons. Light emitting diodes (LEDs) are the most recent lighting technology to enter the controlled environment agriculture arena and have great potential to improve performance and reduce the overall cost of controlled environment lighting. Growth and morphogenetic effects of light (quality, intensity, and duration) and phytohormones are well documented, but their modes of action and mutual interactions are far from clear. One of the important questions is whether at least some of the morphogenetic effects of light are mediated by changes in phytohormone levels. This experiment was conducted in order to study the effect of different light sources on morphology and growth of potato plantlets under in-vitro conditions and their effect on minituber production in greenhouse to decrease energy consumption and production costs.
Materials and Methods: A factorial experiment was conducted based on completely randomized design with six replications under in-vitro conditions and four replications in greenhouse. Factors were consisted of cultivars (cv. Agria and Savalan) and light sources (LEDs emitting red, blue, white, combination of red and blue and also tubular fluorescent lamps as control). The experiment was carried out at the tissue culture laboratory of Yeka Seed Technology of Iran. Disease-free potato in vitro plantlets of Agria and Savalan cultivars were derived from the potato germplasm bank of Agricultural Biotechnology Research Institute of Iran. The plantlets were propagated using single-node cutting. Eight explants were cultured in sterilized culture vessels containing 30 ml of MS and pH was set to 5.8 before adding agar and autoclaving. Culture vessel were closed with polycarbonate caps and sealed with household plastic foil and were placed in a growth chamber at 24 °C and 16 h photoperiod for 4 weeks.
Results and Discussion: Results indicated that red spectrum caused an increase in plantlet height and Agria had a higher plantlet height under in-vitro conditions. Root length was higher in red-blue combination and no difference was observed between cultivars under in-vitro condition. The highest and lowest leaf area was obtained in white and red spectrum, respectively. Also, Savalan showed a higher leaf area in comparison with Agria. The lowest node number in plantlets was observed in red spectrum and the highest in white. The highest and lowest internode length was related to red and blue spectrum, respectively. Fluorescent light was resulted in the lowest plantlet fresh weight and no significant difference was observed among other treatments according to this parameter. Stem diameter and number of branches was not affected by different lights. Results of greenhouse study indicated that light conditions before transplanting of plantlets did not affected minituber number, while minituber weight was significantly affected by this factor and the lowest mean of this parameter was obtained in red spectrum compared to the other treatments.
Conclusion: Generally, results showed that less leaf area plantlet in vitro was also less leaf area in greenhouse (red light emitting diode). Leaf area is an important criteria for photosynthetic and biomass production. Therefore, in this experiment fresh and dry matter production was affected by this characteristics and red light showed the lowest dry matter production. LEDs could be considered as suitable light sources producing plantlets comparable with those grown under fluorescent light under in-vitro conditions along with saving energy and ultimately lower production cost.

Keywords

Agria; Fluorescent; LED; Savalan; spectrum

References

1- Aksenova N.P., Konstantinova T.N., Sergeeva L.I., Machachkova I., and Golyanovskaya S.A. 1994. Morphogenesis of potato plants in-vitro. 1. Effect of light quality and hormones. Journal of Plant Growth Regulation 13:143-146.

2- Avercheva O.V., Berkovich Y.A., Erokhin A.N., Zhigalova T.V., Pogosyan S.I., and Smolyanina S.O. 2009. Growth and photosynthesis of Chinese cabage plants grown under Light- Emiting Diode-based light source. Russian Journal of Plant Physiology 56:14-21.

3- Chindi A., Giorgis G.W., Solomon A., Tessama L., and Negash K. 2014. Rapid multiplication techniques (RMTs): A tool for the production of quality seed potato (Solanum tuberosum L.) in Ethiopia. Asian Journal of Crop Science 6:176-185.

4- Donnelly D.J., Coleman W.K., and Coleman S.E. 2003. Potato micro-tuber production and performance: A review. American Journal of Potato Research 80:103-115.

5- Economou A.S., and Read P.E. 1987. Light treatments to improve efficiency of in vitro propagation system. HortScience 22:751–754.

6- Fillipo R.V.H., Cano G.H.B., and Chaves O.J.A. 2010. LED lighting applications. Science and Technology 45:13-18.

7- Folta K.M. 2004. Green light stimulates early stem elongation, antagonizing light mediated growth inhibition. Plant Physiology 135:1407–1416.

8- Gopal J., Chamail A., and Sarkar D. 2005. Use of microtubers for slow growth in vitro conservation of potato germplasm. Plant Genetic Resources - News 1141:56-60.

9- Haghighi M., and Pessarakli M. 2013. Influence of silicon and nano-silicon on salinity tolerance of cherry tomatoes (Solanum lycopersicum L.) at early growth stage. Scientia Horticulturae 161:111-117.

10- Hayashi M., Fujita N., Kitaya Y., and Kozai T. 1992. Effect of sideward lighting on the growth of potato plantlets in vitro. Acta Horticulturae 319:163-166.

11- Hoagland D.R., and Arnon D.I. 1950. The water-culture method for growing plants without soil. Search Results California Agricultural Experiment Station Circular. 347:1-32.

12- Hogewoning S.W., Trouwborst G., Maljaars H., Poorter H., Ieperen W.V., and Harbinson J. 2010. Blue light dose–responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light. Journal of Experimental Botany 61:3107–3117.

13- Jackson J.A., and Jenkins G.I. 1995. Extension-growth responses and expression of flavonoid biosynthesis genes in the Arabidopsisi hy4 mutant. Plantarum 197:233-239.

14- Kim H.H., Goins G.D., Wheeler R.M., and Sager J.C. 2004. Green light suplementation for enhanced lettuce growth under red- and blue-light-emitting diodes. HortScience 39:1617-1622.

15- Kubasek W.L., Shirley B.W., McKillop A., Goodman H.M., Briggs W., and Ausubel F.M. 1992. Regulation of flavonoid biosynthetic genes in germinating Arabidopsisi seedlings. Plant Cell 4:1229-1236.

16- Miyashita Y., Kimura T., Kitaya Y., and Kozai T. 1994. Effects of red on the growth and morphology of potato plantlets in vitro: An experimental use of light emitting diodes (LEDs) as a light source for tissue culture. Artificial Lighting in Hort. Third International Symposium. Noordwijkerhout, the Netherlands 23–27 Jan.

17- Murashige T., and Skoog F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum 15:473–497.

18- Paniagua Pardo G., Hernandez Aguilar C., Rico Martinez F., Dominguez Pacheco A., Martinez Gonzalez C., and Martinez Canseco M. 2014. Effects of light emitting diode high intensity on growth of lettuce (Lactuca sativa L.) and broccoli (Brassica oleracea L.) seedlings. Annual Review & Research in Biology 4:2983-2994.

19- Perez B.S., Moreno A.D., and Garcia V.C. 2008. Influence of light on health-promoting phytochemicals of broccoli sprouts. Journal of the Science of Food and Agriculture 8: 904–910.

20- Samuoliene G., Brazaityte A., Urbonaviciute A., Sabajeviene G., and Duchovskis P. 2010. The effect of red and blue light component on the growth and development of frigo strawberries. Zemdirbyste 97: 99-104.

21- Schuerger C.A., Brown S.C., and Stryjewski C.E. 1997. Anatomical features of pepper plants (Capsicum annuum L.) grown under red light-emitting diodes supplemented with blue or far-red light. Annals of Botany 79:273-282.

22- Taiz L., and Zeiger E. 2010. Plant Physiology, 5nd Edition Sinauer Associates, Sunderland, MA.

23- Tamulaitis G., Duchovskis P., Bliznikas Z., Breive K., Ulinskaite R., Brazaityte A., Novickovas A., and Zukauskas A. 2005. High-power light-emitting diode based facility for plant cultivation. Journal of Physics D: Applied Physics 38:3182–3187.

24- Tennessen D.J., Singsaas E.L., and Sharkey T.D. 1994. Light-emitting diodes as a light source for photosynthesis research. Photosynthesis Research 39: 85-92.

25- Xu H.I., Xu Q., Li F., Feng Y., Qin F., and Fang W. 2012. Applications of xerophytophysiology in plant production-LED blue light as a stimulus improved the tomato crop. Horticultural Science148:190–196.

26-Yorio N.C., Goins G.D., Kagie H.R., Wheeler M.R., and Sager J.C. 2001. Improving spinach, radish, and lettuce growth under red light emitting diodes (LEDs) with blue light supplementation. Hortscience 36: 380–383.

27- Zhang L., and Wong M.H. 2007. Environmental mercury contamination in China: Sources and impacts. Environment International 33:108-121.

28- Zobayed M., Armstrong J., and Armstrong W. 2001. Micro propagation of potato: Evaluation of closed, diffusive and forced ventilation on growth and tuberization. Annals of Botany 87: 53-59.