Encapsulation of red beetroot extract using spray drying and freeze drying by malto-dexterin and whey protein isolate carriers

مجله پژوهشهای علوم و صنایع غذایی ایران

Article 10, Volume 16, Issue 2 - Serial Number 62, May and June 2020, Pages 313-322   

Document Type: Research Article

DOI: 10.22067/ifstrj.v16i2.81900

Authors

Mahsa Faridnia¹; Ali Mohamadi Sani¹; Masoud Najaf Najafi^{* 2}

¹Department of Food Science & Technology, Quchan Branch, Islamic Azad University, Quchan, Iran.

²Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran.

Abstract

Introduction: Natural substances with plant sources are rich in bioactive and useful compounds that promote health and improve the physicochemical properties of food products. Compounds such as antioxidants, polyphenols, pigments, flavors and etc. are among the most important bioactive plant-based compounds. Red beetroot is one of the unique plants that are rich in antioxidants, anthocyanins, and red pigmentation. The red pigment of the red beetroot is known as betacyanin, and betanin is the major betacyanin compound in red beetroot. Therefore, due to the red beetroot rich in natural pigments, especially betalains, this plant product has a high potential for use as a natural colorant in food, pharmaceutical, and cosmetic products. However, the restrictions on the use of natural and edible pigments are unstable during processing and preservation. The encapsulation process is one of the effective strategies in the food industry to resolve this problem and to protect bioactive compounds during production and storage. Different methods have been used for encapsulation of bioactive compounds, among which the most prominent ones are freeze-drying, emulsion, spray drying, liposomal, niosome, coacervation, and so on. Freeze drying and spray drying are two of the most widely used and industrial methods for the encapsulation of bioactive compounds, pigments, extracts, pharmaceuticals, and microorganisms. So, the aim of this study was to produce red beetroot pulp powder as a source of bioactive and colorant compounds using spray and freeze-drying processes.
 
Materials and methods: Whey protein isolate (WPI) and maltodextrin with a DE 18-20 in different ratios (100: 0, 75:25, 50:50, 25:70 and 0: 100) as wall materials were used to the beetroot extract encapsulation. The spray drying process of the extracts was carried out at an inlet air temperature of 140 °C and outlet air temperature of 65-70 °C with a pump power of 75%, a compressed air flow of 3.5 m3/h, and a feed flow rate of 5 mL/min. To dry the freeze-dried extracts, each sample was first kept at a temperature of -18°C for 3 h, and then the drying operation was performed in a freeze dryer for 24 h. In all treatments, the Brix feed solution was kept constant at 10%. The produced powders were evaluated in terms of encapsulation efficiency, moisture content, water solubility, and total phenol content.
 
Results & discussion: The results showed that by increasing the amount of WPI to 50% (50:50 ratio), encapsulation efficiency significantly increased (p<0.05). In the spray and freeze-dried samples, the lowest moisture content was observed in the sample treated with 100% maltodextrin. Increasing the maltodextrin ratio significantly (p<0.05) increased the solubility of the powders in water. The evaluation of total phenol content showed that the red beetroot extract powders prepared with freeze dryer had the total phenol content. Also, the powder samples obtained from the ratio of 50:50 maltodextrin to WPI had the highest total phenolic content. Based on the results obtained in this study, it was found that the use of the 50:50 ratio for maltodextrin and WPI resulted in desirable physicochemical properties in the powders obtained by both drying methods. Therefore, due to the high costs of freeze-drying and the results of the present study, it can be concluded that the spray drying method with the conditions applied during this study can be used as an effective and promising method for the production of natural pigments based on red beetroot extract for use in food, pharmaceutical, and cosmetic industries. Hence, using a 50:50 ratio for maltodextrin and WPI as wall materials and drying aid is recommended for the production of red beetroot pulp powder.

Keywords

Freeze drying; spray drying; Red beetroot; WPI; Maltodextrin

References

یوسفی، ش. (1397). بهینه سازی فرآیند خشک کردن پاششی جهت تولید پودرهایریزدرونپوشانی شده عصاره فراسودمنداستخراجی از چغندرلبویی. مجله علوم غذایی و تغذیه. سال پانزدهم. شماره 2. 44-31.

AOAC. (2002). Official Methods of Analysis AOAC International, 17thEnd. AOAC International Maryland.

Azeredo, H. (2009). Betalains: properties, sources, applications, and stability–a review. International journal of food science & technology, 44(12), 2365-2376.

Bansal, V., Sharma, H.K., & Nanda, V. (2014). Optimisation of spray drying process parameters for low‐fat honey‐based milk powder with antioxidant activity. International Journal of Food Science & Technology, 49(4), 1196-1202. doi:10.1111/ijfs.12416

Caparino, O. A., Tang, J., Nindo, C. I., Sablani, S. S., Powers, J. R., &Fellman, J. K. (2012). Effect of drying methods on the physical properties and microstructures of mango (Philippine ‘Carabao’var.) powder. Journal of Food Engineering, 111(1), 135-148.

Chen, Q., Zhong, F., Wen, J., McGillivray, D., & Quek, S. Y. (2013). Properties and stability of spray-dried and freeze-dried microcapsules co-encapsulated with fish oil, phytosterol esters, and limonene. Drying Technology, 31(6), 707-716.

Choi, M. J., Briançon, S., Bazile, D., Royere, A., Min, S. G., & Fessi, H. (2007). Effect of cryoprotectant and freeze-drying process on the stability of W/O/W emulsions. Drying technology, 25(5), 809-819.

Du, J., Ge, Z.-Z., Xu, Z., Zou, B., Zhang, Y., & Li, C.-M. (2014). Comparison of the efficiency of five different drying carriers on the spray drying of persimmon pulp powders. Drying Technology, 32.10, 1157-1166.

Fazaeli, M., Emam-Djomeh, Z., KalbasiAshtari. A., & Omid, M. (2012). Effect of spray drying conditions and feed composition on the physical properties of black mulberry juice powder. Food and bioproducts processing, 90(4), 667-675. doi:10.1016/j.fbp.2012.04.006.

Gliszczyńska-Świgło, A., Szymusiak, H., & Malinowska, P. (2006). Betanin, the main pigment of red beet: molecular origin of its exceptionally high free radical-scavenging activity. Food additives and contaminants, 23(11), 1079-1087.

Jayasundera, M., Adhikari, B., Howes, T., & Aldred, P. (2011). Surface protein coverage and its implications on spray-drying of model sugar-rich foods: solubility, powder production and characterization. Food Chemistry, 128.4, 1003-1016.

Jimenez-Aguilar, D. M., Ortega-Regules, A. E., Lozada-Ramirez, J. D., Perez-Perez, M. C. I., Vernon-Carter, E. J., & Welti-Chanes, J. (2011). Color and chemical stability of spray-dried blueberry extract using mesquite gum as wall material. Journal of Food Composition and Analysis, 24(6), 889-894.

Karaca, A. C., Nickerson, M., & Low, N. H. (2013). Microcapsule production employing chickpea or lentil protein isolates and maltodextrin: Physicochemical properties and oxidative protection of encapsulated flaxseed oil. Food Chemistry, 139(1-4), 448-457.

Kaushik, V., & Roos, Y. H. (2007). Limonene encapsulation in freeze-drying of gum Arabic–sucrose–gelatin systems. LWT-Food Science and Technology, 40(8), 1381-1391.

Lakkis, J. M. (2016). Encapsulation and controlled release technologies in food systems. John Wiley & Sons.

Liu, D., Cito, S., Zhang, Y., Wang, C. F., Sikanen, T. M., & Santos, H. A. (2015). A versatile and robust microfluidic platform toward high throughput synthesis of homogeneous nanoparticles with tunable properties. Advanced Materials, 27(14), 2298-2304.

Liu, W., Chen, X. D., Cheng, Z., &Selomulya, C. (2016). On enhancing the solubility of curcumin by microencapsulation in whey protein isolate via spray drying. Journal of Food Engineering, 169, 189-195.

Mani, S., Jaya, S., & Das, H. (2002). Sticky issues on spray drying of fruit juices. Paper presented at the ASAE/CSAEC North Central Intersectional Meeting, Parktown Hotel, Saskatoon, Saskatchewan, Canada, September pp. 27-28

Manickavasagan, A., Thangavel, K., Dev, S. R. S., Delfiya, D. A., Nambi, E., Orsat, V., & Raghavan, G. S. V. (2015). Physicochemical characteristics of date powder produced in a pilot-scale spray dryer. Drying Technology, 33(9), 1114-1123.

Nadeem, H. Ş., Torun, M., & Özdemir, F. (2011). Spray drying of the mountain tea (Sideritisstricta) water extract by using different hydrocolloid carriers. LWT-Food Science and Technology, 44(7), 1626-1635.

Najaf Najafi, M., Kadkhodaee, R., & Mortazavi, S. A. (2011). Effect of drying process and wall material on the properties of encapsulated cardamom oil. Food biophysics, 6(1), 68-76.

Neelwarne, B., & Rudrappa, T. (2013). Peroxidases and Other Enzymes from Red Beet Hairy Roots. In Red Beet Biotechnology (pp. 283-333). Springer US.

Otalora, M. C., Carriazo, J. G., turriaga, L. I., Nazareno, M. A. & osorio, C. (2015). Microencapsulation of betalains obtained from cactus fruit (Opuntiaficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents. Food Chemistry, 187, 174-181.

Potter, N. N., & Hotchkiss, J. H. (2012). Food science. Springer Science & Business Media.

Rezaeinia, H., Ghorani, B., Emadzadeh, B., & Tucker, N. (2019). Electrohydrodynamic atomization of Balangu (Lallemantiaroyleana) seed gum for the fast-release of Menthalongifolia L. essential oil: Characterization of nano-capsules and modeling the kinetics of release. Food Hydrocolloids, 93, 374-385.

Sablani, S. S., Shrestha, A. K., & Bhandari, B. R. (2008). A new method of producing date powder granules: Physicochemical characteristics of powder. Journal of Food Engineering, 87(3), 416-421.

Santhalakshmy, S., Bosco, S. J. D., Francis, S., & Sabeena, M. (2015). Effect of inlet temperature on physicochemical properties of spray-dried jamun fruit juice powder. Powder Technology, 274, 37-43.

Shefer, A., & Shefer, S. (2003). Novel encapsulation system provides controlled release of ingredients. Food Technology.

Sogi, D. S., Siddiq, M., Greiby, I., & Dolan, K. D. (2013). Total phenolics, antioxidant activity, and functional properties of ‘Tommy Atkins’ mango peel and kernel as affected by drying methods. Food chemistry, 141(3), 2649-2655.

Tavares, G. M., Croguennec, T., Carvalho, A. F., & Bouhallab, S. (2014). Milk proteins as encapsulation devices and delivery vehicles: applications and trends. Trends in Food Science & Technology, 37.1, 5-20.