The effect of chitosan-whey protein based edible coating containing bionanocomposite material and Zataria multiflora essential oil on UF-Feta type cheese shelf life

مجله پژوهشهای علوم و صنایع غذایی ایران

Article 5, Volume 17, Issue 5 - Serial Number 71, November and December 2021, Pages 729-745   

Document Type: Research Article

DOI: 10.22067/ifstrj.v17i5.88681

Authors

Maryam Gohargani¹; Hannan Lashkari^{* 2}; Alireza Shirazinejad¹

¹Department of Food Science and Technology, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran.

²Department of Food Science and Technology, Zarindasht Branch, Islamic Azad University, Zarindasht, Iran.

Abstract

Introduction: In recent years, the tendency to use antimicrobial edible film and coating has increased, which has increased the quality, safety and shelf life of food. Cheese is one of the most important dairy products that has a special nutritional value in human nutrition. UF-Feta cheese, which is a type of cheese, is contaminated by microorganisms such as coliforms, spore-forming bacteria and lactose-fermenting yeasts. The causative agent of listeriosis, Listeria monocytogenes, is transmitted through the consumption of cheese. In this study, the effect of composite edible coating based on chitosan and whey protein containing titanium dioxide (TiO₂) nanoparticles and Zataria multiflora essential oil on shelf life, microbial, physicochemical and sensory properties of UF-Feta type cheese was investigated.  Furthermore, the inhibitory effect of films from coating solutions on the growth of Listeria monocytogenes was also investigated.
 
Materials and Methods: Chitosan, whey protein isolate (WPI) (higher than 91% protein), Zataria multiflora essential oil (ZEO), TiO₂ nanoparticles, and glycerol were procured from Bio Basic (Canada), Hilmar Canada, Barij-Essence Co. (Iran), Acros Co. (USA), and Merck Co. (Darmstadt, Germany), respectively. In order to prepare the coatings, a solution of WPI and chitosan was prepared separately. Whey protein suspension (3%, w/v) was made by dispersing WPI in DDW subsequently heated at 90°C for 30 min at pH value of 8.0 and then cooled rapidly. Chitosan solution (10 g/L) was made by dispersing chitosan in 2% (v/v) acetic acid solution with constant mixing for 3 h at 60°C. Based on preliminary experiments, whey protein–chitosan suspension was made using blending two polymer suspensions at constant ratio of WPI/chitosan (70:30) and mixed magnetically for 15 min at 25ºC. In the next step, TiO₂ NPs (1 and 2% w/w) were incorporated and after mixing for 15 min, glycerol (30% w/w) was incorporated to the composite suspension and again stirred for 30 min. Next, ZEO (0 and 1% v/v) was incorporated into the composites suspension and sonicated for 30 min with power of 100 W.
To produce cheese samples, milk was subjected to bactofogation, pasteurization (76°C for 15 seconds), ultrafiltration and then the retentate was homogenized at a pressure of 70 bars. By adding a starter culture (10 units /1000 L), the pH of the retentate was decreased to 6.2. Then, rennet (0.004 g/ 100 g) and salt (2 g/ 100 kg) were mixed with water and added to the cheese container. Retentate was transferred to a coagulation tunnel at 37 ° C for 30 minutes to form a coagulum.  After incubation, the coating solutions were sprayed on the clot using a spray device equipped with a spray gun and packed with aluminum foil. Finally, the samples were stored at 4°C. Samples were subjected to microbiological tests (contaminating microbes and starters), physicochemical (fat, titratable acidity, pH, and moisture content, texture analysis) and sensory evaluation at different ripening periods (3 to 60 days). To evaluate the growth inhibitory of Listeria monocytogenes on the surface of cheese by composite films, pieces of cheese (23× 21× 1.7 mm) were first cut under aseptic conditions and their upper surface was inoculated with 40 μl of Listeria monocytogenes (ATCC19115) until the initial bacterial count was about 3.5 log cfu /g. The composite films placed on the surface of the inoculated cheese and stored at 4°C. Film-inoculated cheese samples were used as a control. Microbial counts were done at intervals of days 0, 3, 8, 11, 14. Data analyzed with SPSS software and means were compared with Duncan multiple range test.
 
Results and Discussion: The results showed that TiO₂ NPs and ZEO significantly reduced total bacterial count, lactic acid bacteria and coliforms compared to control with increasing storage period. Mold and yeast colonies also increased considerably over time in the control compared to the nanoparticle-treated samples, while the While in treatments containing ZEO did not grow at all. Physicochemical analysis exhibited that the content of moisture, fat, and pH of all cheeses decreased, acidity and texture hardness increased. Sensory evaluation of UF-Feta cheeses showed that the aroma, taste and overall acceptability of the control and nanoparticle-containing coatings improved compared to the brine-treated cheese. Nevertheless, in the coating samples containing ZEO due to the negative effect of the essential oil on the organoleptic properties of cheese, consumer acceptance was significantly reduced. On the other hand, the use of composite films to inhibit the growth of Listeria monocytogenes on the surface of UF-Feta cheese at 4 °C for 14 days showed that composite films, especially films containing ZEO, had a significant effect on the reduction of the Listeria monocytogenes population. As a conclusion, composite films containing TiO₂ NPs and ZEO could be applied in food packaging systems, particularly at the UF-Feta cheese packaging.

Keywords

Zataria multiflora essential oil; Whey protein isolate; UF-Feta Cheese; Coating; Chitosan; TiO2 nanoparticles

References

استاندارد ملی ایران. 1385. شیر و فراورده های آن-تعیین اسیدیته و ph-روش آزمون. شماره 2852.

استاندارد ملی ایران. 1393. محصولات پنبر و پنیر فراوری شده -اندازه گیری میزان چربی -روش وزن سنجی (روش آزمون مرجع). شماره 17602.

استاندارد ملی ایران. 1378. روش ارزیابی حسی بستنی. شماره 4937.

جمشیدی ف. رحیمی س. فدائی نوغانی و. (1397). مطالعه اثر فیلم خوراکی ژل آلوئه ورا- صمغ فارسی بر ویژگی­های پنیر سفید ایرانی. مجله علوم تغذیه و صنایع غذایی ایران، 13 (1)1: 63-74.

رمضانی ا. جلیل زاده ع. حصاری ج. (1395). تاثیر پوشش خوراکی بر پایه پروتئین آب پنیر و ناتامایسین بر کیفیت و ماندگاری پنیر سفید ایرانی. نشریه حوزه سلامت و بهداشت مواد غذایی، 23: 12- 1.

روشنی س. گوهری اردبیلی ا. آریان فر ا. (1394). بررسی اثر آنتی اکسیدانی و ضدمیکروبی اسانس آویشن بر پنیر موزارلای نگهداری شده در دمای یخچال. نشریع پژوهش و نوآوری در علوم و صنایع غذایی، 3: 246-233.

قره محمدلو ا. جلیل زاده ع. حصاری ج. (1395). تاثیر پوشش خوراکی بر پایه کنسانتره پروتئین آب­پنیر حاوی نیسین روی ماندگاری پنیر سفید آب نمکی. مجله علوم و صنایع غذایی،  14(66): 269-261.

Artiga-Artigas, M., Acevedo-Fani, A. & Martín-Belloso, O., 2017, Improving the shelf life of low-fat cut cheese using nanoemulsion-based edible coatings containing oregano essential oil and mandarin fiber. Food Control, 76, 1-12.‏

Berti, S., Resa, C. P. O., Basanta, F., Gerschenson, L. N. & Jagus, R. J., 2019, Edible coatings on Gouda cheese as a barrier against external contamination during ripening. Food Bioscience, 31, 100447.‏

Cano Embuena, A. I., Cháfer Nácher, M., Chiralt Boix, A., Molina Pons, M. P., Borrás Llopis, M., Beltran Martínez, M. C. & González Martínez, C., 2017, Quality of goat′ s milk cheese as affected by coating with edible chitosan‐essential oil films. International Journal of Dairy Technology, 70(1), 68-76.‏

Conte, A., Gammariello, D., Di Giulio, S., Attanasio, M. & Del Nobile, M. A., 2009, Active coating and modified-atmosphere packaging to extend the shelf life of Fior di Latte cheese. Journal of Dairy Science, 92(3), 887-894.‏

Conte, A., Scrocco, C., Sinigaglia, M. & Del Nobile, M. A., 2007, Innovative active packaging systems to prolong the shelf life of mozzarella cheese. Journal of Dairy Science, 90(5), 2126-2131.‏

Del Nobile, M. A., Gammariello, D., Conte, A. & Attanasio, M., 2009, A combination of chitosan, coating and modified atmosphere packaging for prolonging Fior di latte cheese shelf life. Carbohydrate polymers, 78(1), 151-156.‏

Di Pierro, P., Sorrentino, A., Mariniello, L., Giosafatto, C. V. L. & Porta, R., 2011, Chitosan/whey protein film as active coating to extend Ricotta cheese shelf-life. LWT-Food Science and Technology, 44(10), 2324-2327.‏

Divsalar, E., Tajik, H., Moradi, M., Forough, M., Lotfi, M. & Kuswandi, B., 2018, Characterization of cellulosic paper coated with chitosan-zinc oxide nanocomposite containing nisin and its application in packaging of UF cheese. International Journal of Biological Macromolecules, 109, 1311-1318.‏

Gammariello, D., Di Giulio, S., Conte, A. & Del Nobile, M. A., 2008, Effects of natural compounds on microbial safety and sensory quality of Fior di Latte cheese, a typical Italian cheese. Journal of Dairy Science, 91(11), 4138-4146.‏

Hannon, J. A., Lopez, C., Madec, M. N. & Lortal, S., 2006, Altering renneting pH changes microstructure, cell distribution, and lysis of Lactococcus lactis AM2 in cheese made from ultrafiltered milk. Journal of Dairy Science, 89(3), 812-823.‏

Li, W., Zheng, K., Chen, H., Feng, S., Wang, W. & Qin, C., 2019, Influence of nano titanium dioxide and clove oil on chitosan–starch film characteristics. Polymers, 11(9), 1418.

Lotfi, M., Tajik, H., Moradi, M., Forough, M., Divsalar, E. & Kuswandi, B., 2018, Nanostructured chitosan/monolaurin film: Preparation, characterization and antimicrobial activity against Listeria monocytogenes on ultrafiltered white cheese. LWT- Food Science and Technology, 92, 576-583.‏

Martins, J. T., Cerqueira, M. A., Souza, B. W., Carmo Avides, M. D. & Vicente, A. A., 2010, Shelf life extension of ricotta cheese using coatings of galactomannans from nonconventional sources incorporating nisin against Listeria monocytogenes. Journal of Agricultural and Food Chemistry, 58(3), 1884-1891.‏

Meira, S. M. M., Zehetmeyer, G., Scheibel, J. M., Werner, J. O. & Brandelli, A., 2016, Starch-halloysite nanocomposites containing nisin: Characterization and inhibition of Listeria monocytogenes in soft cheese. LWT-Food Science and Technology, 68, 226-234.‏

Mushtaq, M., Gani, A., Gani, A., Punoo, H. A. & Masoodi, F. A., 2018, Use of pomegranate peel extract incorporated zein film with improved properties for prolonged shelf life of fresh Himalayan cheese (Kalari/kradi). Innovative Food Science & Emerging Technologies, 48, 25-32.‏

Nottagh, S., Hesari, J., Peighambardoust, S. H., Rezaei-Mokarram, R. & Jafarizadeh-Malmiri, H., 2019, Effectiveness of edible coating based on chitosan and Natamycin on biological, physico-chemical and organoleptic attributes of Iranian ultra-filtrated cheese. Biologia, 75(4), 605-611.

Nottagh, S., Hesari, J., Peighambardoust, S. H., Rezaei-Mokarram, R. & Jafarizadeh-Malmiri, H., 2018, Development of a biodegradable coating formulation based on the biological characteristics of the Iranian Ultra-filtrated cheese. Biologia, 73(4), 403-413.‏

Pluta-Kubica, A., Jamróz, E., Kawecka, A., Juszczak, L. & Krzyściak, P., 2019, Active edible furcellaran/whey protein films with yerba mate and white tea extracts: Preparation, characterization and its application to fresh soft rennet-curd cheese. International Journal of Biological Macromolecules, 155, 1307-1316.

Ramos, Ó. L., Pereira, J. O., Silva, S. I., Fernandes, J. C., Franco, M. I., Lopes-da-Silva, J. A. & Malcata, F. X., 2012, Evaluation of antimicrobial edible coatings from a whey protein isolate base to improve the shelf life of cheese. Journal of Dairy Science, 95(11), 6282-6292.‏

Soleimani-Rambod, A., Zomorodi, S., Naghizadeh Raeisi, S., Khosrowshahi Asl, A. & Shahidi, S. A., 2018, The effect of Xanthan gum and flaxseed mucilage as edible coatings in cheddar cheese during ripening. Coatings, 8(2), 80.‏

Wang, Q., Yu, H., Tian, B., Jiang, B., Xu, J., Li, D. & Liu, C., 2019, Novel Edible Coating with Antioxidant and Antimicrobial Activities Based on Whey Protein Isolate Nanofibrils and Carvacrol and Its Application on Fresh-Cut Cheese. Coatings, 9(9), 583.‏

Yangilar, F., 2017, Effects of natamycin edible films fortified with essential oils on the safety and quality parameters of Kashar cheese. Journal of Food Safety, 37(2), e12306.‏

Yilmaz, F. & Dagdemir, E., 2012, The effects of beeswax coating on quality of Kashar cheese during ripening. International Journal of Food Science & Technology, 47(12), 2582-2589.‏

Yilmaz, G., Ayar, A. & Akin, N., 2005, The effect of microbial lipase on the lipolysis during the ripening of Tulum cheese. Journal of Food Engineering, 69(3), 269-274.‏

Youssef, A. M., El-Sayed, S. M., Salama, H. H., El-Sayed, H. S. & Dufresne, A., 2015, Evaluation of bionanocomposites as packaging material on properties of soft white cheese during storage period. Carbohydrate Polymers, 132, 274-285.‏

Youssef, A. M., El-Sayed, S. M., El-Sayed, H. S., Salama, H. H. & Dufresne, A., 2016, Enhancement of Egyptian soft white cheese shelf life using a novel chitosan/carboxymethyl cellulose/zinc oxide bionanocomposite film. Carbohydrate Polymers, 151, 9-19.‏

Youssef, A. M., Assem, F. M., Abdel-Aziz, M. E., Elaaser, M., Ibrahim, O. A., Mahmoud, M. & Abd El-Salam, M. H., 2019, Development of bionanocomposite materials and its use in coating of Ras cheese. Food chemistry, 270, 467-475.

Zantar, S., Yedri, F., Mrabet, R., Laglaoui, A., Bakkali, M. & Zerrouk, M. H., 2014, Effect of Thymus vulgaris and Origanum compactum essential oils on the shelf life of fresh goat cheese. Journal of Essential Oil Research, 26(2), 76-84.‏

Zhong, Y., Cavender, G. & Zhao, Y., 2014, Investigation of different coating application methods on the performance of edible coatings on Mozzarella cheese. LWT-Food Science and Technology, 56(1), 1-8.‏

Ziaee, E., Razmjooei, M., Shad, E. & Eskandari, M. H., 2018, Antibacterial mechanisms of Zataria multiflora Boiss. essential oil against Lactobacillus curvatus. LWT, 87, 406-412.