Experimental and Theoretical Investigation of Young's Modulus of Breast Cancer Tissue (MCF-10) Using Different Cantilevers of Atomic Force Microscope

علوم کاربردی و محاسباتی در مکانیک

Article 4, Volume 36, Issue 1 - Serial Number 35, April 2024, Pages 61-76   

Document Type: Original Article

DOI: 10.22067/jacsm.2023.83806.1196

Authors

Moein Taheri^{* 1}; Ali Jabbari²; Zahrasadat Eghdami²; hamed faraji³; Nima Rahmani³

¹Mechanical Engineering, Arak University, Arak, Iran.

²Department of Manufacturing, Faculty of Engineering, Arak University, Arak, Iran.

³Department of Mechanical Engineering, Faculty of Engineering, Arak University, Arak

Abstract

Today, the atomic force microscope has various applications in the manufacture of small-scale parts and the study of their mechanical properties. The study of mechanical properties of tissues can be considered as biomarkers for early detection of cancer and help in new treatments. There are diferent ways to detect cancerous tissues, and one of these ways is to check Young’s modulus of the tissue. One of the most recent methods for extracting Young's modulus in biological tissues is the use of atomic force microscopy. In this study, atomic force microscope was first used to extract Young's modulus of MCF-10 breast cancer tissue using 3 different cantilevers with rectangular, V-shaped and dagger geometries. The geometry of the cell was also assumed to be spherical according to the images obtained by atomic force microscopy. The force- indentation depth diagram was plotted by averaging the experimental results for each of the cantilevers separately. Finally, Young's modulus of breast cancer tissue for 3 cantilevers with rectangular, V-shaped and dagger geometries is extracted with Hertz contact model. By comparing the experimental and theoretical results and by changing the assumed range of Young's modulus for all 3 geometries of the cantilevers, it was observed that the use of V-shaped pole predicts a more accurate range of Young's modulus due to applying less force to the tissue. Young's modulus of breast cancer was considered between 1200 and 1250 (Pa) using V-shaped cantilevers.

Keywords

Atomic force microscope; MCF-10 cancer tissue; Rectangular cantilevers; Dagger cantilevers; V-shaped cantilevers

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