A preliminary molecular study of the Iranian species of Calomyscus (Rodentia- Calomyscidae) using RFLP

Iranian Journal of Animal Biosystematics

مقاله 4، دوره 5، شماره 2، 2009، صفحه 33-41   

نوع مقاله: Research articles

شناسه دیجیتال (DOI): 10.22067/ijab.v5i2.3337

نویسندگان

B. Sahebjam¹؛ Jamshid Darvish^{* 2}؛ Eskandar Rastegar Pouyani³؛ Roohollah Siahsarvie²؛ Safie Akbarirad¹

¹Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

²Rodentology Research Department, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

³Department of Biology, Faculty of Science, University of Sabzevar, Sabzevar, Iran

چکیده

The Calomyscidae is a small family of Rodentia that has recently been classified into 8 species in a single genus, Calomyscus. Currently 5 species are reported in Iran: C. elburzensis,، C. bailwardi، C. grandis ، C. urartensis and C. hotsoni. Species discrimination based on morphological characteristics is difficult, so this study re-evaluated the phylogenetic relationships and taxonomic status of the morphotypes within Calomyscus using PCRRFLP as a molecular marker. Forty seven samples of Calomyscus spp from 9 geographically separated regions of Iran were analyzed. The cytochrome b gene was amplified by PCR, digested using 3 restriction enzymes, HinfI, HincII and HaeIII, and the patterns separated electrophoretically. Species of long tailed hamster were discriminated from one another. A phylogenetic analysis of the dataset produced well distinguished clades.

کلیدواژه‌ها

Calomyscus؛ Cytochrome b؛ RFLP