Application of RAPD (Random Amplified Polymorphic DNA) marker for sex determination of Pistacia vera L.

Journal of Cell and Molecular Research

مقاله 1، دوره 1، شماره 2، مهر 2009، صفحه 68-71   

نوع مقاله: مقاله

شناسه دیجیتال (DOI): 10.22067/jcmr.v1i2.3221

نویسندگان

Aliakbar Ehsanpour^* ؛ Lila Arab

University of Isfahan

چکیده

Sex determination in Pistacia as a dioecious plant is economically desirable. Identification of the male and
female plants has a great value for Pistacia yield production. In the present study, leaf samples from four
female plants from cultivars of Pistacia including Akbary, Akbar Aghaei, Fandoghi, and Kalleh Ghochi as well
as male plants were used for RAPD PCR amplification. Among 32 primers with 10 mer two primers (FPKI106
and FPK105) were able to discriminate between female and male plants. Few bands were detected in the DNA
pattern of male while they were absent in the female individuals. The PCR conditions were reproducible and
can be recommended for sex determination of Pistacia vera cultivars.

کلیدواژه‌ها

Pistacia vera؛ RAPD-PCR؛ sex determination