Effects of the Theranekron®”an alcoholic extract of the Tarantula cubensis” on hematology and serum biochemical properties in horses

Iranian Journal of Veterinary Science and Technology

مقاله 2، دوره 3، شماره 2، آبان 2011، صفحه 9-16   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v3i2.17858

نویسندگان

Kamran Sardari^* ؛ Mehrdad Mohri؛ Sadaf Sabzevari؛ Behrooz Fathi

Ferdowsi University of Mashhad

چکیده

Theranekron®is commercially available, alcoholic extract of the tarantula cubensis(brown spider).Ten healthy thoroughbred mare racehorses were used at the present study. Blood samples were taken 30 minutes before and 8, 24, 48, 72 and 168h aftersubcutaneousadministration of 10ml Theranekron (1mg/48kg or 0.02mg/kgbw) via a jugular catheter. The results of this study showed that sampling time had a significant effects on the amount of PCV, hemoglobin concentration, RBC number, total protein, albumin, glucose, cholesterol, BUN, creatinine, bilirubin, activity of ALT, and ALP (p0.05). In conclusion, most of the observed changes in hematological and serum biochemical parameters were statistically and not clinically significant. Thus it seems that administration of Theranekron has no adverse reaction in experimental horses.

کلیدواژه‌ها

brown spider؛ venom؛ horse؛ theranekron؛ tarantula cubensis

مراجع

Aguiyi, J.C., Guerranti, R., Pagani, R. and Marrinello, E. (2001) Blood chemistry of rats pretreated with Mucunapruriens

seed aqueous extract MP101UJ after Echiscarinatus venom challenge.Phytotherapy Research 8, 712-714.

Cam, Y., Kibar, M., Atasever, A., Atalay, O.and Beyaz, L. (2007)Efficay of levamisole and Tarantula cubensis venom for the treatment of bovine papillomatosis.The Veterinary Record

160, 486-488.

Cousin, X. and Bon, C. (1999) Acetylcholinesterase from snake

venom as a model for its nerve and muscle counterpart. Journal of Natural Toxins 8, 285-294.

da Silva, P.H., Hashimato, Y., dos Santos,F.B., Mangili, O.C., Gremski, W. and Veiga, S.S. (2003) Hematological cell findings in bone marrow and peripheral blood of rabbits after experimental

acute exposure to Loxoscelesintermedia (brown spider)

venom. Toxicon 42, 155-161.

Diochot, S., Loret, E., Bruhn, T., Beress, L.and Lazdunski, M. (2003) APET×1, a new toxin from the sea anemone

Anthopleura elegantissima, blocks voltage-gated human ether-a-go-gorelated gene potassiumchannels.Molecular Pharmacology 64, 59-69.

Dounay, A.B. and Forsyth, C.J. (2002) Okadaic acid: the archetypal serine/threonine protein phosphatase inhibitor. Current Medical Chemistry 9, 1939-1980.

El-Asmar, M.F., Shaban, E., Hagag, M.,Swelam, N. and Tu, A. (1986).Coagulant component in Cerastescerastes (Egyptian sand viper) venom. Toxicon 24, 1037-1044.

Embergs, H. and Sensen, B. (2007)Effectiveness of a homeopathicn treatment of chronic endometritis in dairy cows. Praktische Tierarzt 88,534-537.

Flachsenberger, W., Leigh, C.M. and Mirtschin, P.J. (1995) Spheroecinocytosis of human red blood cells caused by snake, red-back spider, bee and blue-ringed octopus venoms and its

inhibition by snake sera. Toxicon 33,791-797.

Fry, B.G., Wickramaratana, J.C. and Lemme,S. (2005) Novel natriuretic peptides from the venom of the inland taipan

(Oxyuranusmicrolepidotus): isolation, chemical and biological

characterization. Biochemical Biophysical Research Communication

327 (4), 1011-1015.

Futrell, J. (1992) Loxoscelism. Americal Journal ofthe Medical Sciences 304, 261-267.

Honkanen, R.E. and Golden, T. (2002) Regulators of serine/threonine protein phosphatases at the dawn of a clinical

era. Current Medical Chemistry 9,2055-2075.

Ibrahim, A. Al-Jammaz. (2003) Physiological effects of LD50 of Echiscoloratus crude venom on rat different time intervals. Journal of King Saudi University 15, Science (2), 135-143.

Koch, H. and Stein, M. (1980) Conservative and surgical treatment of panaritium in cattle by using theranekron. Der

praktische tierarzt Jhrg 61, Heft 2;116-117.

Lewis, R.J. and Garcia, M.L. (2003) Therapeutic potential of venom peptides. Nature Reviews Drug Discovery 10, 790-802.

Li-Smerin, Y. and Swartz, K.J. (2001)Helical structure of the COOH terminus of S3 and its contribution to the gating modifies toxin receptor in voltagegated ion channels. The Journal of

General Physiology 117, 205-217.

Mezger, J. (1977) Prospected homeopathic pharmaceutical product device. Issue II 1418-1421.

Milne, T.J., Abbenante, G., Tyndall, J.D.,Halliday, J. and Lewis, R.J. (2003) Isolation and characterization of a cone snail protease with homology to CRISP proteins of the pathogenesis-related protein superfamilly. The Journal of Biological Chememistry 278, 31105-31110.

Muhammad M.A. Salman. (2009) Physiological effects of envenomation by two different doses of the viper

Echiscoloratusis crude venom on biochemical parameters in serum of Ginea pigs at different times. Egyptian Academic Journal of Biological Sciences 1, (1), 21-31.

Olivera, B.M., Mclntosh, J.M., Cruz, L.J.,Luque, F.A. and Gray, W.R. (1984) Purification and sequence of a presynaptic peptide toxin from Conusgeographus venom. Biochemistry 23, 5087-5090.

Sardari, K., Galedar, E. and Mohri, M.(2007)Evaluation of wound contraction and epithelialization after subcutaneous

administration of Theranekron® in cows. Comparative Clinical Pathology 16, 97-102.

Stampa, S. (1986) A field trial comparing the efficacy of sulfamonomethoxine,penicillin, and tarantula poison in the

treatment of pododermatitiscircumspecta of cattle.Journal of the South African Veterinary Association, June; 91-93.