Effect of free and symbiotic nitrogen fixing bacterial co-inoculation on seed and seedling of soybean seeds produced under deficit water condition

بوم شناسی کشاورزی

Article 4, Volume 1, Issue 1, March 2009   

Document Type: Scientific - Research

DOI: 10.22067/jag.v1i1.2654

Authors

Hamed Hadi^{* 1}; Jahanfar Daneshian²; Ahmad Asgharzadeh³; Aydin Hamidi⁴; Parisa Jonoubi⁵; Farshad Ghooshchi¹; Mohammad Nasri¹

¹Islamic Azad University, Varamin Branch, Varamin , Iran

²Seed and Plant Improvement Institue, Agricultural Research, Education, and Extension Organization, Karaj, Iran

³Soil and Water Institute, Iran

⁴Seed and Plant Certification and Registration Institute, Karaj, Iran

⁵Department of Biology, Tarbiat Moalem University, Iran

Abstract

Effect of free and symbiotic nitrogen fixing bacteria on seed and seedling produced seeds under deficit irrigation was conducted in laboratory and field experiments in 2006. In laboratory of karaj’s Seed and Plant Research and Certificate Institute an experiment was conducted based on factorial in form of completely randomized design with four replications and in field’s of Islamic Azad University, Varamin Branch were split factorial in form of randomized completely block design with three replications. Treatments included water stress [Irrigation after 50 (Normal irrigation), 100 (Middle stress), 150 (Severe stress) mm evaporation from pan class A], Cultivar [Manokin & Williams and SRF×T3 Line] and inoculation [Inoculation with Bradyrhizobium japonicum, Bradyrhizobium japonicum co-inoculated with Azotobacter chroococcum, No seed inoculation]. Results showed that drought stress decreased the uniformity and germination speed and seedling emergence. Bacteria increased leaf dry weight, stem dry weight, leaf area and seedling vigor index but had no effect on emergence. In irrigation levels inoculated treatments had higher seedling length, leaf, stem, seedling dry weight and seedling vigor. Severs stress seeds inoculated with Bradyrhizobium japonicum had higher root dry weight than control. Therefore in seeds which were produced under deficit irrigation conditions, bacteria increased seedlings vigor.

Keywords

Seedling vigor; Seedling emergence; Bradyrhizobium japonicum; Azotobacter chroococcum

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