Proline, sodium and potassium concentration changes in gamma rays and NaCl

Journal of Cell and Molecular Research

Article 3, Volume 2, Issue 2 - Serial Number 4, January 0, Pages 74-80   

DOI: 10.22067/jcmr.v2i2.10762

Authors

احسان پور احسان پور^* ; Behrokh Shojaie; Mohammad Reza Abdi

Abstract

In this study, the effects of gamma rays on changes of proline, sodium and potassium in potato calli
(Solanum tuberosoum L.) cultivar "White Desiree" were investigated. After irradiation of calli by 60 and 80 Gy
of gamma radiation, irradiated and non irradiated calli were transferred to a callus production medium
containing 0, 30, 60, 90, 120 and 150 mM NaCl. After 26 days post treatment amounts of proline, Na+ and K+
were measured. The results showed that generally in each of gamma ray treatment, amounts of proline at 120
and 150 mM NaCl were significantly more than 0, 30, 60 and 90 mM salt. A significant difference observed
only at 150 mM NaCl between irradiated and non irradiated calli. This result could be related to the proline
role in osmotic adjustment at high concentration of salt. The amounts of sodium increased by increasing salt
the concentration but the amount of potassium decreased. These results showed that the sodium influx
inhibited the potassium uptake, and may be because of strong inhibitory effect of sodium on the potassium
uptake system or efflux potassium from the cells. The Na+/K+ ratio decreased at 150 mM by 60 and 80 Gy of
gamma radiation.

Keywords

Callus; Gamma ray; Proline; Potato