Changes in anti-oxidant activity of Thymus transcaspicus (Klokov) during

Journal of Cell and Molecular Research

مقاله 2، دوره 3، شماره 1 - شماره پیاپی 5، شهریور 2011، صفحه 12-18   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v3i1.13111

نویسندگان

Narjes Zamani؛ Manijeh Mianabadi^* ؛ Ahmad Abdolzadeh

چکیده

Antioxidant activities protect the cell against oxidative agents that are constant metabolic by-products. The
aim of this study was to investigate the relationship between harvesting time of Thymus transcaspicus and its
antioxidant activities. The plant samples were harvested 5 times in different growth phases from 17 April to 22
July 2008, and its antioxidant activity was studied using the ferric reducing antioxidant power (FRAP), 1,1-
diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, and β-carotene bleaching (BCB) assays.
The results of FRAP assay indicated that the reduction activity of the plant was in the highest level in stage 5
of sampling. The result of DPPH assay showed that the crude extract of the plant was more capable of DPPH
radical scavenging in stage 2. The highest level of gallic acid and quercetin in the crude extract of T.
transcaspicus was determined as 85.29 ± 6.22 mg and 18.88 ± 0.9 mg in stage 2, respectively. Therefore, stage
2 was the optimum time to harvest the T. transcaspicus.

کلیدواژه‌ها

Thymus transcaspicus؛ antioxidant capacity؛ Growth stages؛ total phenolic content؛ total flavonoid