Cloning and expression analysis of Arabidopsis TRR14 gene under salt and drought stress

Journal of Cell and Molecular Research

مقاله 1، دوره 4، شماره 1 - شماره پیاپی 7، شهریور 2012، صفحه 1-10   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v4i1.12269

نویسندگان

Mahnaz Aghdasi^* ؛ Fariba Fazli؛ Mohammad Bagher Bagherieh

چکیده

TRR14 is a novel protein important in trehalose (α-D-glucosyl-[1,1]-α-D-glucopyranoside) signaling in Arabidopsis. In this research, we provided evidences to demonstrate that TRR14 plays role in Arabidopsis responses to salt and drought stress.
The transgenic Arabidopsis plants over-expressing TRR14 under the control of CaMV 35S promoter were generated. Transformed lines showed higher transcript levels of TRR14 than that of Wild Type (WT) Arabidopsis plants. The RT-PCR results showed that TRR14 transcript level increased markedly by salt and drought stress both in WT and transformed lines. Further experiments indicated that the TRR14 transformed lines have unchanged seed germination, root length and chlorophyll content under stress conditions. In addition activity of oxidative enzymes like peroxidase and catalase were significantly induced in tranformed lines under salt and drought treatments. Thus, the present data indicate that a novel protein, TRR14, is involved in plant salt and drought tolerance.

کلیدواژه‌ها

TRR14؛ over-expression؛ Arabidopsis؛ Salt؛ drought