Effects of Gonadotropin Releasing Hormone (GnRH) on bovine spermatogonial stem cell proliferationatogonial stem cells proliferation

Iranian Journal of Veterinary Science and Technology

مقاله 2، دوره 6، شماره 2، 2014، صفحه 11-20   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v6i2.21007

نویسندگان

Parviz Tajik¹؛ Hesam Kohsari^{* 1}؛ Babak Qasemi-Panahi²؛ Iraj Sohrabi-Haghdoost³؛ Abbas Barin⁴؛ Hamid Ghasemzadeh-Nava⁴

¹Islamic Azad University

²University of Tabriz

³Science and Research‌ Branch, Islamic Azad University

⁴University of Tehran

چکیده

Identification of exogenous factors affecting spermatogonial stem cells (SSCs) proliferation in vitro, provides worthy ways to study the basic biology of the cells. The aim of this study was to investigate the effects of a GnRH analogue (alareline acetate) on SSCs colonization in short-term co-culture with sertoli cells. Five, three-month old Holstein male calves were used to isolate spermatogonial and sertoli cells. Testicular germ cell collection was made by enzymatic digestion methods. The cells were co-cultured in a 15 day period and in vitro effects of various doses (0.5, 1, 2 and 4 µg/ml) of GnRHa on SSCs colonization were assessed. Effects of GnRHa on SSCs proliferation were dose dependent. In conclusion, it was demonstrated that 1 µg/ml GnRHa was the optimum dose for SSCs colonization in comparison with control group. The highest treatment dose (4 µg/ml GnRHa), negatively affected SSCs colonization in comparison with control group.

کلیدواژه‌ها

GnRH؛ Calf؛ Spermatogonial Stem Cell؛ Sertoli cell؛ Co-culture

مراجع