Histological Changes of Carrot (Daucus carota L.) Petiole Somatic Embryogenesis in some Media

علوم باغبانی

Article 12, Volume 25, Issue 1, September 2011   

Document Type: Research Article

DOI: 10.22067/jhorts4.v1390i1.9754

Authors

Seyyed Javad Mousavizadeh^* ; Kambiz Mashayekhi

Department of Horticultural Sciences, Gorgan University of agricultural sciences and natural resources, Gorgan, Iran.

Abstract

Abstract
Somatic embryogenesis is a new approach to in vitro plant propagation. This technique is based on stem cell obtain and its differentiation to a complete plant. This is affected by components of media culture. So, the effects of MS, B5 and NL media on carrot petiole somatic embryogenesis investigated via histological experiment. To do this, carrot petioles (with 1 cm dimension) cultured in liquid phase of these media. Different concentrations of IAA and 2,4-D were used for induction of somatic embryogenesis. For histological study, after induction phase, sampling from cultured petioles was done and fixed in FAA solution. After dehydration, paraffin embedding, blocking, sectioning and staining, samples were used to examine the specimen for the documentation. Results indicated that there is significant differences among MS, B5 and NL media with respect to carrot somatic embryogenesis (P