Transient Expression of Coat Protein of Foot and Mouth Disease Virus (FMDV) in Alfalfa (Medicago sativa) by Agroinfiltration

Journal of Cell and Molecular Research

مقاله 6، دوره 8، شماره 2 - شماره پیاپی 16، اسفند 2016، صفحه 83-89   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v8i2.44884

نویسندگان

Maziar Habibi-Pirkoohi^{* 1}؛ Saeid Malekzadeh-Shafaroudi²؛ Hasan Marashi²؛ Saeid Zibaee³؛ Afsaneh Mohkami⁴؛ Saba NejatizaXeh²

¹Ferdowsi University of Mashhad

²Department of Biotechnology and plant breeding, Ferdowsi University of Mashhad, Mashhad, Iran

³Razi Vaccine and Serum Research Institute, Ministry of Agriculture, Mashhad, Iran

⁴Horticulture research Institute, Shahid Bahonar University, Kerman, Iran

چکیده

An Agrobacterium-mediated transient gene expression assay was carried out in alfalfa (Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via agroinfiltration procedure. The presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain reaction (PCR), real time PCR, protein Dot blot and ELISA. Moreover, gene expression in the transformed leaves was quantified by ELISA method. The results obtained in this investigation indicated high level of gene expression in alfalfa leaves, showing that transient gene expression can be applied as an effective and time-saving procedure for the production of recombinant proteins. The procedures for transformation, detection of recombinant protein and its application for molecular experiments are described in the study.

کلیدواژه‌ها

Agroinfiltration؛ FMDV؛ Recombinant vaccine؛ Alfalfa؛ VP1

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