Analyses of genetic variation in Khorasan indigenous chicken breed by using of microsatellite mark

پژوهشهای علوم دامی ایران

Article 3, Volume 1, Issue 2, June 2009   

Document Type: Scientific - Research Articles

DOI: 10.22067/ijasr.v1i2.1971

Authors

Mahdi Nikbakhti^{* 1}; Hamid Reza Mirzaee¹; Majid Afsharian Shandiz²; Mohammadreza Mohammad Abadi³; Davoud Ali Saghi⁴

¹Department of Animal Science, Zabol Faculty of Agriculture, Zabol, Iran

²Razi Vaccine Serum Research Institute

³Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

⁴Khorasan Agricultural and Natural Resources Research and Training Center

Abstract

This study investigated genetic variation within indigenous chicken of Khorasan using four microsatellite markers (MCW5,
MCW16, MCW18 and MCW34). 90 blood samples were collected from this population and phenol-chloroform extraction
method was used to isolate the DNA. The highest and the lowest allele number and effective alleles are shown in MCW5 (6
and 5.293) and MCW34 (4 and 3.138), respectively. The expected heterozygosis varied from 0.8157 (MCW5) to 0.6854
(MCW34). The amount of genetic variation within this population was found 0.74 this could be due to low inbreeding in this
population. The Hardy-Weinberg equilibrium (HWE) test showed that all loci deviated from HWE (p

Keywords

Genetic variation; Microsatellite marker; Indigenous chicken

References

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